To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effect...To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.展开更多
[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was us...[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.展开更多
基金supported by Research Foundation for Science and Technology Innovation Talents of Harbin(2010RFQXS072)Scientific Research Foundation for the Returned Overseas Chinese Scholars of Heilongjiang Province of China(200923)
文摘To get the IR spectrums of Agrimonia Pilosa Ledeb （APL） from China and Japan areas, and to fend out the characters of IR spectrums through the content of different chemical constituents, to provide a fast and effective analysis method monitor the inherent qualities of traditional Chinese medicine-APL. Fourier Transform Infrared Spectroscopy （FT-IR） was applied to detect sample of APL from Ofina and Japan areas. This study showed that the IR spectra of APL from China and Japan areas have their unique IR fingerprint features. The contents of tannin and calcitum phosphate in APL from China is different APL from Japan. So FF-IR is a very quick, effective and well repetitive method for monitoring and distinguishing the traditional Chinese medicine.
文摘[ Objectives] This study was conducted to compare agrimophol contentsin Agrimonia pilosa Ledeb. at different harvest time, so as to select its best harvest time. [Methods] High performance liquid chromatography was used for the determination of agfmophol in A. pilosa Ledeb. at different harvest time in Luzhou. [ Results ] The content of agrimophol could be detected by HPLC. Agrimophol had good linearity in 0.01 - 0.1 mg/ml （ r = 0.999 9 ）, the average recov- ery was 101.5%, and RSD was 1.10%. The content of agrimophol in A. pilosa Ledeb. in Luzhou was 0.010 8% in June, and 0.013 1% in July, much higher than any other months. [ Conclusions] This experiment established a method for detecting the content of agrimophol by HPLC, which is simple, with high precision, high accuracy, good repeatability and recovery. The reasonable harvest time of A. pilosa Ledeb. in Luzhou is June and July.
