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Natural isothiocyanates of the genus Capparis as potential agonists of apoptosis and antitumor agents:Mechanisms and implications
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作者 Nabil Eid Payal Bhatnagar 《World Journal of Pharmacology》 2024年第1期1-7,共7页
In this editorial,we comment on a recent publication,which highlights the important findings from the study,including the antitumor and anti-inflammatory effects of isothiocyanates,their underlying mechanisms,and impl... In this editorial,we comment on a recent publication,which highlights the important findings from the study,including the antitumor and anti-inflammatory effects of isothiocyanates,their underlying mechanisms,and implications.Additionally,a related perspective is discussed. 展开更多
关键词 Cancer isothiocyanateS Genus Capparis APOPTOSIS AUTOPHAGY INFLAMMATION GC/MS analysis
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Natural isothiocyanates of the genus Capparis as potential agonists of apoptosis and antitumor drugs 被引量:1
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作者 Lumír Hanuš Tuvia Naor +1 位作者 Tatyana Gloriozova Valery M Dembitsky 《World Journal of Pharmacology》 2023年第4期35-52,共18页
BACKGROUND Using gas chromatography-mass spectrometry(GC/MS)analysis,we examined the composition of volatile components present in the yellow and green fruits,seeds,and jam of the scrambling shrub Capparis cartilagine... BACKGROUND Using gas chromatography-mass spectrometry(GC/MS)analysis,we examined the composition of volatile components present in the yellow and green fruits,seeds,and jam of the scrambling shrub Capparis cartilaginea(C.cartilaginea).These plant samples were collected from Kibbutz Yotvata in Israel.In all the tested samples,isothiocyanates were identified.Utilizing the PASS program,we ascertained the biological activity of these isothiocyanates present in the Capparis genus.The study results highlighted that all isothiocyanates could potentially act as apoptosis agonists,making them strong candidates for antitumor drugs.This information holds significant value for the fields of medicinal chemistry,pharmacology,and practical medicine.AIM To investigate the volatile components present in the yellow and green fruits,seeds,and jam of the C.cartilaginea shrub using GC/MS analysis,to detect isothiocyanates in all the analyzed plant samples,and to assess the biological activity of these isothiocyanates utilizing the PASS program.METHODS We utilized two primary methods to analyze the volatile compounds present in the yellow and green fruits,seeds,and jams of the C.cartilaginea,native to Israel.We identified biologically active isothiocyanates in these samples.Their anticipated biological activities were determined using the PASS program,with the most dominant activities being apoptosis agonist,anticarcinogenic,and antineoplastic specifically for genitourinary cancer.RESULTS Fruits,seeds,and jams containing isothiocyanates,which exhibit antineoplastic and anticarcinogenic activities,could be suggested for cancer prevention and management.Specific isothiocyanates,with therapeutic potential in this realm,could be recommended as potent anticancer agents in practical medicine following clinical trials.CONCLUSION The discovery that isothiocyanates exhibit potent antineoplastic and anticarcinogenic activities was unexpected.Additionally,certain isothiocyanates demonstrated antifungal,antiviral(specifically against arbovirus),and antiparasitic properties. 展开更多
关键词 Capparis cartilaginea FRUITS Seeds isothiocyanateS APOPTOSIS ANTICANCER
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Allyl isothiocyanate ameliorates lipid accumulation and inflammation in nonalcoholic fatty liver disease via the Sirt1/AMPK and NF-κB signaling pathways 被引量:22
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作者 Chun-Xiao Li Jian-Guo Gao +9 位作者 Xing-Yong Wan Yi Chen Cheng-Fu Xu Ze-Min Feng Hang Zeng Yi-Ming Lin Han Ma Ping Xu Chao-Hui Yu You-Ming Li 《World Journal of Gastroenterology》 SCIE CAS 2019年第34期5120-5133,共14页
BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct ... BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct impact on the liver.AIM To investigate the effect and underlying mechanism of AITC in nonalcoholic fatty liver disease(commonly referred to as NAFLD).METHODS To establish a mouse and cellular model of NAFLD,C57BL/6 mice were fed a high fat diet(HFD)for 8 wk,and AML-12 cells were treated with 200μM palmitate acid for 24 h.For AITC treatment,mice were administered AITC(100 mg/kg/d)orally and AML-12 cells were treated with AITC(20μmol/L).RESULTS AITC significantly ameliorated HFD-induced weight gain,hepatic lipid accumulation and inflammation in vivo.Furthermore,serum alanine aminotransferase and aspartate aminotransferase levels were markedly reduced in AITC-treated mice.Mechanistically,AITC significantly downregulated the protein levels of sterol regulatory elementbinding protein 1(SREBP1)and its lipogenesis target genes and upregulated the levels of proteins involved in fatty acidβ-oxidation,as well as the upstream mediators Sirtuin 1(Sirt1)and AMPactivated protein kinaseα(AMPKα),in the livers of HFD-fed mice.AITC also attenuated the nuclear factor kappa B(NF-κB)signaling pathway.Consistently,AITC relieved palmitate acid-induced lipid accumulation and inflammation in AML-12 cells in vitro through the Sirt1/AMPK and NF-κB signaling pathways.Importantly,further studies showed that the curative effect of AITC on lipid accumulation was abolished by siRNA-mediated knockdown of either Sirt1 or AMPKαin AML-12 cells.CONCLUSION AITC significantly ameliorates hepatic steatosis and inflammation by activating the Sirt1/AMPK pathway and inhibiting the NF-κB pathway.Therefore,AITC is a potential therapeutic agent for NAFLD. 展开更多
关键词 ALLYL isothiocyanate NONALCOHOLIC fatty LIVER disease Hepatic STEATOSIS LIVER INFLAMMATION
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Antioxidant Activity of Isothiocyanate Extracts from Broccoli 被引量:5
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作者 袁海娜 姚善泾 +2 位作者 尤玉如 肖功年 尤琦 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第2期312-321,共10页
Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical( 2 O·-) ,hydroxyl radical(HO·-)... Isothiocyanates(ITCs) extracts were prepared from fresh broccoli.Their antioxidant properties were evaluated by using the in vitro bioassays,including superoxide anion radical( 2 O·-) ,hydroxyl radical(HO·-) and 2,2-diphenyl-1-picrylhydracyl(DPPH·) radical-scavenging methods,lipid peroxidation assay,and reducing power assay.The ITCs extracts exhibited significant dose-dependent antioxidant activities(P<0.01) .Its antioxidant-stability was affected by temperature and storage condition,although it was more stable than vitamin C(Vc) .GC-MS iden-tified sulforaphane in the ITCs extracts.The antioxidant activity of sulforaphane was approximately one tenth to one fifth of that of Vc.Sulforaphane was experimented to be one of dominating contributors to antioxidant capacity of ITCs extracts.However,there was no direct antioxidant activity found for benzyl isothiocyanate(BITC) in vitro. Therefore,it could be concluded that the general group(-N=C=S) was not the essential part for the antioxidant activity of sulforaphane.It is suggested that the ITCs extracts from broccoli can be developed into a promising food additive for healthy food. 展开更多
关键词 antioxidant activity isothiocyanateS BROCCOLI SULFORAPHANE
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Electro-optical properties of high birefringence liquid crystal compounds with isothiocyanate and naphthyl group 被引量:1
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作者 彭增辉 王启东 +7 位作者 王少鑫 姚丽双 刘永刚 胡立发 曹召良 穆全全 杨程亮 宣丽 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第9期242-247,共6页
Liquid crystal(LC) compound with isothiocyanate and naphthyl group is an attractive high birefringence LC material,and can be used in optical devices. In this paper, the electro-optical properties of a series of thi... Liquid crystal(LC) compound with isothiocyanate and naphthyl group is an attractive high birefringence LC material,and can be used in optical devices. In this paper, the electro-optical properties of a series of this type of LC compounds were investigated. The melting points and enthalpy values of these LC compounds were higher than those of corresponding compounds with the phenyl group. These compounds exhibited high birefringence with a maximum value of 0.66. Fluorine substitution in the molecular almost does not affect the birefringence value. When these LC compounds with the naphthyl group were dissolved in a commercial LC mixture, the electro-optical properties depending on temperature were investigated. In the low-temperature region, LC mixtures with the naphthyl-group LC compounds exhibited higher viscosity than pure commercial LCs. In the high-temperature region, viscosity values very closely approached each other. When response performance was investigated, figure-of-merit(FoM) values were measured. The Fo M values of LC mixtures containing LC compounds with naphthyl group were lower than those of reference benzene LCs in the low-temperature region. However, in the high-temperature region, the results were reversed. These isothiocyanate LC compounds with naphthyl group can be applied in special fast-response LC device, particularly the ones used under high-temperature conditions. 展开更多
关键词 naphthalene isothiocyanate liquid crystals high birefringence electro-optical property
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Preparation and Performance of Fluorescein Isothiocyanate⁃Labeled Fluorescent Starch and Polyvinyl Alcohol for Warp Sizing 被引量:1
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作者 LI Manli JI Zhihao +2 位作者 ZOU Zhuanyong HU Qiaoling JIN Enqi 《Journal of Donghua University(English Edition)》 CAS 2021年第5期424-433,共10页
In order to solve the problems of low accuracy and poor variety adaptability of the current measurement method of the permeability and coating property of sizing paste in textile industry, taking starch and polyvinyl ... In order to solve the problems of low accuracy and poor variety adaptability of the current measurement method of the permeability and coating property of sizing paste in textile industry, taking starch and polyvinyl alcohol(PVA) as the representatives of the most commonly used textile sizes, various concentrations of fluorescent molecules fluorescein isothiocyanate(FITC) were used to label starch and PVA to prepare fluorescein(F)-starch and F-PVA fluorescent sizes with different degrees of labeling(DLs) for the first time, respectively. Then the starch and PVA derivatives were employed to size pure cotton warp yarns. After preparing the sections of the sized yarns, the permeability and coating percentage of starch and PVA paste to the yarns were calculated by using a fluorescence microscope and the Photoshop software, respectively. The results demonstrate that F-starch and F-PVA with appropriate DL of 0.791% and 0.161%, respectively, exhibit good fluorescence property and similar sizing performance to the sizing performance of unlabeled starch and PVA. It is considered that fluorescence labeling of sizing agents with fluorescein units can provide an innovative way for the accurate determination of the permeability and coating property of sizing paste to warp yarns. 展开更多
关键词 fluorescence labeling fluorescein isothiocyanate(FITC) PERMEABILITY coating property textile size
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Studies on Rare Earth Complexes with Crown Ether (ⅩⅩⅣ)——Synthesis and Characterization of Complexes of Lanthanide Isothiocyanates with Dibenzo-30-crown-10
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作者 LU Tong-bu, GAN Xin-min, TANG Ning and TAN Min-yu (Department of Chemistry, Lanzhou University, Lanzhou, 730000) 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1992年第3期319-323,共5页
Introduction The study of metal complexes with the crown ether is a quite interesting research field, but the research has mainly focused on the metal complexes with some crown ethers with a relatively small cavity su... Introduction The study of metal complexes with the crown ether is a quite interesting research field, but the research has mainly focused on the metal complexes with some crown ethers with a relatively small cavity such as 12-crown-4, 15-crown-5 and 18- crown-6 as well as their derivatives. Crown ethers with a larger cavity contain more oxygen atoms, so they have the ability to form the complexes of a higher co- 展开更多
关键词 Lanthanide isothiocyanates Crown ethers COMPLEXES SYNTHESIS
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Synthesis and Determination of Standard Molar Enthalpies of Formation for Complexes of Rare Earth Isothiocyanates with Glycine
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作者 Yin Jingzhi, Jiang Bengao and Yang Shurong (School of Chemistry, Shandong University, Jinan) 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 1990年第1期56-61,共6页
Four solid complexes of rare earth isothiocyanates with glycine were synthesized. They were characterized by chemical analysis, elemental analysis, Infrared spectra, X-ray powder diffraction and TO-DSC analysis. Their... Four solid complexes of rare earth isothiocyanates with glycine were synthesized. They were characterized by chemical analysis, elemental analysis, Infrared spectra, X-ray powder diffraction and TO-DSC analysis. Their chemical formulae were proved to be RE(NCS)2.Gly .H2O, where RE is La, Ce, Pr or Nd. The integral heats of solution of RE(NCS)3.3Gly. H2O in water, of RE(NCS)2. 7H2O in aqueous glycine solution and of glycine in water have been measured calorimetricaliy at 298. 15K. By means of a thermochemical cycle suggested in this paper, the standard molar enthalpies of formation for RE(NCS)2. 3Gly.H2O(c) were obtained and their lattice energies were calculated. 展开更多
关键词 Rare earth isothiocyanate GLYCINE Standard molar enthalpy of formation.
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Nonlinear Intestinal Absorption of Fluorescein Isothiocyanate Dextran 4, 000 Caused by Absorptive and Secretory Transporting System
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作者 Mikio Tomita Rie Ohkubo +2 位作者 Shohei Ouchi Chise Kawahata Masahiro Hayashi 《Pharmacology & Pharmacy》 2011年第3期173-179,共7页
The mechanism of the nonlinear concentration dependence of the intestinal absorption of fluorescein isothiocyanate dextran 4,000 (FD-4) was studied using in situ rat intestinal loops and the in vitro Ussing-type chamb... The mechanism of the nonlinear concentration dependence of the intestinal absorption of fluorescein isothiocyanate dextran 4,000 (FD-4) was studied using in situ rat intestinal loops and the in vitro Ussing-type chamber method. The intestinal absorption rate constant of FD-4, as evaluated by the intestinal loop method, increased significantly in a nonlinear fashion as the FD-4 concentration increased up to 0.2 mM and tended to decrease at concentrations higher than 0.2 mM. The mucosal-to-serosal permeation of FD-4 across rat ileal sheets, as evaluated by the in vitro Ussing-type chamber method, also increased in a nonlinear fashion in the low concentration range (0.01 - 0.02 mM), before decreasing as the concentration increased further, whereas serosal-to-mucosal permeation decreased in a concentration-dependent manner. In addition, mucosal-to-serosal flux and serosal-to-mucosal flux were increased and reduced in the presence of the metabolic inhibitor 2, 4-dinitrophenol, respectively. These results suggest that FD-4 is predominantly secreted into the intestinal lumen by an efflux transport system. 展开更多
关键词 FLUORESCEIN isothiocyanate DEXTRAN 4 000 NONLINEAR Absorptive TRANSPORT NONLINEAR SECRETORY TRANSPORT Rat Ileal Intestine
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Synthesis of ethoxycarbonyl isothiocyanate by orthogonal design
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作者 梁文杰 钟宏 何谋海 《Journal of Central South University》 SCIE EI CAS 2012年第9期2447-2450,共4页
Using Schiff base as a phase transfer catalyst, ethoxycarbonyl isothiocyanate was synthesized by reacting ethyl chloroformate with sodium thiocyanate. In order to get the best synthetic technology, an orthogonal test ... Using Schiff base as a phase transfer catalyst, ethoxycarbonyl isothiocyanate was synthesized by reacting ethyl chloroformate with sodium thiocyanate. In order to get the best synthetic technology, an orthogonal test (L9(34)) was applied. The results show that reaction temperature, reaction time, content of catalyst and molar ratio of sodium thiocyanate to ethyl chloroformate are the main factors influencing the yield. The four factors chosen for the present investigation are based on the results of a single-factor test. The optimum synthetic technology is determined as follows: reaction temperature 35 ℃, reaction time 3 h, the content of catalyst (molar fraction based on ethyl chloroformate) 1.5% and molar ratio of sodium thiocyanate to ethyl chloroformate 1.1. Under the optimized synthetic technology, the experimental yield reaches 96.8%. 展开更多
关键词 ethoxycarbonyl isothiocyanate Schiffbase phase transfer catalyst orthogonal test
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REACTION OF α-(1H-1,2,4-TRIAZOL-1-YL)ACETOPHENONE WITH PHENYL ISOTHIOCYANATE
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作者 Zheng Ming LI Zhen Nian HUANG Institute of Elemento-Organic Chemistry,Nankai University,Tianjin,300071 《Chinese Chemical Letters》 SCIE CAS CSCD 1993年第9期763-766,共4页
Reaction of α-(1H-1,2,4-triazol-1-yl)acetophenone with phenyl isothiocyanate in the presence of a base and subse- quent alkylation leads to the ketene S,N-acetal(2),(4),(7), (8);thiazolidone(3),thiazolidine(5)and 1,3... Reaction of α-(1H-1,2,4-triazol-1-yl)acetophenone with phenyl isothiocyanate in the presence of a base and subse- quent alkylation leads to the ketene S,N-acetal(2),(4),(7), (8);thiazolidone(3),thiazolidine(5)and 1,3-thiazine(6), 展开更多
关键词 PH TRIAZOL-1-YL)ACETOPHENONE WITH PHENYL isothiocyanate REACTION OF REV
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Tissue distribution of Lycium barbarum polysaccharides in rat tissue by fluorescein isothiocyanate labeling
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作者 Chao Yang Hui Xia +2 位作者 Huali Tang Ligang Yang Guiju Sun 《Food Science and Human Wellness》 SCIE 2022年第4期837-844,共8页
To date, in vivo investigations of polysaccharide’s pharmacokinetics are significantly restricted by the difficulty in their detection. This study was conducted to establish the quantitative determination of Lycium b... To date, in vivo investigations of polysaccharide’s pharmacokinetics are significantly restricted by the difficulty in their detection. This study was conducted to establish the quantitative determination of Lycium barbarum polysaccharides(LBPs) based on fluorescein isothiocyanate(FITC) pre-labeling and to investigate their tissue distribution in rat. We obtained the calibration curves linear over the range of 0.0–25 μg/m L in rat tissue samples with correlation coefficients greater than 0.99. The inter-day and intra-day precisions(RSD, %) were within 15%, and the relative recovery ranged 95.2%–102.4%, with RSD range 1.48%–9.58%, indicating that this experiment was suitable for the determination of LBPs. The fluorescence intensity was measured after 24 h storage at room temperature, 3 times of freeze-cycle and cryopreservation at –20 ℃ for 15 day, these results indicated that the stability of the samples was good. LBP-FITC was mainly absorbed by the small intestine and stomach, and mainly excreted in the urine through the kidney;this distinct difference in the tissue distribution of LBPs could be attributed to the size of these LBPs in relation to the pore sizes of the vascular beds in the kidney and liver. Results showed in this study enable us to comprehensively understand the biological effects of LBPs following its oral ingestion. 展开更多
关键词 Lycium barbarum polysaccharides Fluorescein isothiocyanate Tissue distribution
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Impact of Soil Texture and Organic Matter Content on Methyl Isothiocyanate Volatilization from Soil Columns
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作者 C. R. Simpson S. D. Nelson H. A. Ajwa 《Journal of Agricultural Science and Technology(A)》 2011年第2X期194-198,共5页
Metam sodium (MS; sodium N-methyl dithiocarbamate) has emerged as a promising soil fumigant in the US to replace methyl bromide (MeBr). Metam potassium (MK; potassium N-methyl dithiocarbamate) and MS break down ... Metam sodium (MS; sodium N-methyl dithiocarbamate) has emerged as a promising soil fumigant in the US to replace methyl bromide (MeBr). Metam potassium (MK; potassium N-methyl dithiocarbamate) and MS break down into the volatile gas methyl isothiocyanate (MITC) to control soil borne pests. Many studies have focused on MS, but MK has not been studied as thoroughly. The objective of this research was to determine the effect of increasing organic matter (OM) treatments and soil texture to minimize the off-gassing of MS and MK. Bench-scale soil column studies were performed to simulate organic matter treatments that may decrease the volatilization loss of MITC. Incorporation depth of OM simulated surface tillage (0-15 cm) practices. Soil was packed in steel columns and MS or MK was applied at a depth of 15 cm and MITC volatilization was measured using gas chromatography/mass spectroscopy. Volatilization of MITC behaved similarly for MS and MK with MITC movement impacted by soil texture. MITC volatilization was lower from a sandy clay loam than a sandy soil. Surface incorporation of OM did not significantly decrease MITC volatilization. These results suggest that soil texture is the dominant factor reducing MITC off-gassing and prolonging the time needed to control soil borne pests. 展开更多
关键词 Metam sodium metam potassium methyl isothiocyanate methyl bromide alternatives soil columns.
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Isothiocyanates Induce Apoptosis in Human Non-Small Cell Lung Cancer Cells through a Redox-Mediated Mechanism
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作者 Ke XU Yu ZHU Xiang WU Huiqin YAN Qinghua ZHOU 《中国肺癌杂志》 CAS 2009年第6期471-472,共2页
Purpose Lung cancer is one of the most aggressive cancer types, and is the leading cause of cancer-related deaths worldwide. Isothiocyanates are natural compounds found in
关键词 肺癌 系统评价 治疗 紫极类药物
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苯基异硫氰酸酯类液晶材料的稳定性
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作者 彭增辉 刘永刚 穆全全 《液晶与显示》 CAS CSCD 北大核心 2024年第11期1431-1436,共6页
苯基异硫氰酸酯化合物可制成向列相液晶材料。这类材料不仅具有高双折射率,还具有低黏度的特性,在液晶光学器件中应用时可实现快速响应。然而,这类液晶材料存在稳定性问题,导致其制成的液晶器件无法长时间稳定运行。本文研究发现,在加... 苯基异硫氰酸酯化合物可制成向列相液晶材料。这类材料不仅具有高双折射率,还具有低黏度的特性,在液晶光学器件中应用时可实现快速响应。然而,这类液晶材料存在稳定性问题,导致其制成的液晶器件无法长时间稳定运行。本文研究发现,在加速老化实验中,100℃处理1000 h后,该材料的双折射率下降了8.5%。通过高效液相色谱法和质谱法分析,确认羟基化合物会引起异硫氰酸酯基团的离解,导致材料稳定性欠佳。最后,在设计的无水环境下进行老化实验,结果显示材料的降解程度大幅降低,在相同条件下双折射率仅下降1.1%。本研究为这类材料的应用提供了重要的指导方案。 展开更多
关键词 液晶材料 异硫氰酸酯 高双折射率 稳定性
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药液稀释倍数对威百亩分解产生异硫氰酸甲酯及其消解的影响 被引量:1
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作者 陈新华 唐秀军 +6 位作者 张毅 方文生 颜冬冬 李园 曹坳程 白庆荣 王秋霞 《植物保护》 CAS CSCD 北大核心 2024年第1期122-128,共7页
种植前施用威百亩进行土壤熏蒸消毒,其分解产生的异硫氰酸甲酯(methyl isothiocyanate, MITC)可有效控制土传病原菌。威百亩以滴灌方式施用更为方便、安全,但在滴灌前的稀释过程中可能会水解产生MITC,MITC继续消解或挥发导致进入土壤的... 种植前施用威百亩进行土壤熏蒸消毒,其分解产生的异硫氰酸甲酯(methyl isothiocyanate, MITC)可有效控制土传病原菌。威百亩以滴灌方式施用更为方便、安全,但在滴灌前的稀释过程中可能会水解产生MITC,MITC继续消解或挥发导致进入土壤的有效成分量减少。因此有必要筛选适宜的威百亩稀释倍数,保证MITC在土壤中分布均匀的同时减少威百亩分解及MITC消解和挥发导致的损失。本文研究了不同pH条件下稀释倍数对威百亩水解产生MITC及MITC消解的影响。结果表明,在中性条件下,由威百亩水解产生的MITC量及其消解速率均随稀释倍数增加而增加;在酸性条件下,稀释100倍时MITC的产生量及消解速率均为最大,稀释50倍时MITC的产生量最低,稀释400倍时MITC消解最慢;在碱性条件下,MITC的产生量随稀释倍数增加而增加,消解速率为稀释1 000倍时最快,稀释100倍时最慢。研究结果对滴灌施用威百亩时田间用水量及威百亩用量均具有指导意义。 展开更多
关键词 威百亩 异硫氰酸甲酯 滴灌施药 稀释倍数 消解
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异硫氰酸苄酯通过激活p53和AMPK-FOXO1a信号通路诱导宫颈癌细胞周期阻滞和凋亡 被引量:2
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作者 塔玛莎·库尔曼江 王小静 +7 位作者 李欣奕 王昊 解国轩 陈雲杰 温婷 程夕露 努尔阿米乃·麦麦提 李金玉 《中国药理学通报》 CAS CSCD 北大核心 2024年第1期114-125,共12页
目的 研究异硫氰酸苄酯(benzyl isothiocyanate, BITC)对小鼠U14宫颈癌细胞增殖的影响,基于转录组数据分析探讨细胞毒性机制。方法 MTT法检测BITC对U14细胞活性的影响,Hochest 33258和荧光倒置显微镜检测细胞核形态变化,流式细胞术检测... 目的 研究异硫氰酸苄酯(benzyl isothiocyanate, BITC)对小鼠U14宫颈癌细胞增殖的影响,基于转录组数据分析探讨细胞毒性机制。方法 MTT法检测BITC对U14细胞活性的影响,Hochest 33258和荧光倒置显微镜检测细胞核形态变化,流式细胞术检测细胞周期和凋亡,基于Illumina Novaseq 6000测序平台建立BITC(20μmol·L^(-1))给药前后U14细胞转录组数据库,使用生物信息学分析手段对差异基因功能和信号通路进行富集分析。qRT-PCR和Western blot验证信号通路关键分子表达变化。结果 BITC呈时间和浓度依赖性抑制U14细胞的活性,阻滞细胞在G_(0)/G_(1)期,诱导了细胞凋亡,上调了Bax、CytC、cleaved caspase-9/3、下调了Bcl-2、cleaved caspase-7的表达量;通过转录组分析,差异基因主要富集在细胞周期、FOXO、p53、细胞凋亡等信号通路,qRT-PCR或Western blot验证p53、p21、Ire1a、Atf4、CHOP、AMPK、FOXO1a等分子表达上调,Cyclin D3,Cyclin E,Cdk2分子表达下调。结论 BITC阻滞U14细胞周期、通过caspase-3线粒体依赖途径和内质网应激途径诱导细胞凋亡,其机制与p53和AMPK-FOXO1a信号通路有密切关系。 展开更多
关键词 异硫氰酸苄酯 宫颈癌 转录组 P53 FOXO 细胞周期 细胞凋亡
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异硫氰酸苄酯对荧光假单胞菌的抑菌活性及在牛肉保鲜中的应用
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作者 葛子同 刘佳楠 +3 位作者 张淑琪 毕景然 侯红漫 张公亮 《肉类研究》 北大核心 2024年第5期27-35,共9页
荧光假单胞菌(Pseudomonas fluorescens)广泛存在于牛肉、鱼类、家禽和乳制品等生鲜产品中,成为引发食源性疾病的重要原因。以荧光假单胞菌ATCC 13525作为供试菌株,研究异硫氰酸苄酯(benzyl isothiocyanate,BITC)对菌株的抑制作用及其... 荧光假单胞菌(Pseudomonas fluorescens)广泛存在于牛肉、鱼类、家禽和乳制品等生鲜产品中,成为引发食源性疾病的重要原因。以荧光假单胞菌ATCC 13525作为供试菌株,研究异硫氰酸苄酯(benzyl isothiocyanate,BITC)对菌株的抑制作用及其应用于牛肉的保鲜效果。结果表明:通过牛津杯实验法,得到BITC对荧光假单胞菌的最小抑菌浓度(minimum inhibitory concentration,MIC)为0.25 mmol/L;表型实验表明BITC能够破坏细菌细胞膜的通透性并降低代谢活力;此外,相比于对照组,MIC处理组牛肉贮藏7 d内菌落总数、色差值、pH值、总挥发性盐基氮含量及氮氧化合物含量分别降低35.73%、21.52%、4.53%、54.39%和4.78%,而感官评分提高10.45%,说明BITC可以显著抑制荧光假单胞菌的生长,改善牛肉的感官品质及理化指标。因此,BITC在冷鲜牛肉贮藏保鲜方面具有较好的应用前景。 展开更多
关键词 荧光假单胞菌 异硫氰酸苄酯 牛肉 抑菌作用 保鲜效果
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AITC调控肠嗜铬细胞中血清素的代谢及其生物学功能
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作者 王思瑜 杨雅丽 +2 位作者 司琪琦 郭泰林 黄新河 《生物学杂志》 CAS CSCD 北大核心 2024年第2期51-56,共6页
为探讨异硫氰酸烯丙酯(Allylisothiocyanate,AITC)调控肠嗜铬细胞(Enterochromaffin cells,EC)中血清素(5-hydroxytryptamine,5-HT)的合成及其对EC生物学功能的影响,使用食用级AITC对RIN-14B细胞(大鼠胰腺内分泌细胞系)进行干预处理,采... 为探讨异硫氰酸烯丙酯(Allylisothiocyanate,AITC)调控肠嗜铬细胞(Enterochromaffin cells,EC)中血清素(5-hydroxytryptamine,5-HT)的合成及其对EC生物学功能的影响,使用食用级AITC对RIN-14B细胞(大鼠胰腺内分泌细胞系)进行干预处理,采用FLUO-8 AM检测RIN-14B细胞内钙离子浓度,qRT-PCR分析5-HT合成相关基因表达,UPLC测定5-HT含量,以及RNA-seq检测AITC处理后的RIN-14B细胞转录组,并对其结果进行生物信息学分析。结果表明,AITC通过激活离子通道TRPA1使胞内钙离子浓度升高,上调Tph1(色氨酸羟化酶)和Ddc(5-羟色氨酸脱羧酶)的表达,从而促进5-HT合成及分泌。此外,GO和KEGG功能富集分析AITC处理后的RIN-14B细胞差异表达基因发现,AITC主要调控谷胱甘肽代谢和抗氧化、炎症调节等相关通路,提示AITC可能通过刺激EC促进谷胱甘肽代谢来调节肠道稳态,同时参与肠道的炎症调节。以上结果为研究AITC影响EC及肠道更深入的生物学功能提供实验数据与研究思路。 展开更多
关键词 异硫氰酸烯丙酯 肠嗜铬细胞 血清素 RNA-SEQ 生物学功能
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不同药剂对书虱的毒力及其复配与联用增效作用
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作者 陈雨昂 涂艳清 +2 位作者 冯华月 王进军 魏丹丹 《中国粮油学报》 CAS CSCD 北大核心 2024年第6期13-19,共7页
为书虱的抗性治理提供科学指导,本研究采用培养皿药膜法测定了4种储粮保护剂对5种书虱的触杀毒力,并采用广口瓶密闭熏蒸法测定了8种植物精油对嗜卷书虱的熏蒸活性。同时,评估了药剂复配及其与辣根素精油联用对嗜卷书虱的协同毒力。结果... 为书虱的抗性治理提供科学指导,本研究采用培养皿药膜法测定了4种储粮保护剂对5种书虱的触杀毒力,并采用广口瓶密闭熏蒸法测定了8种植物精油对嗜卷书虱的熏蒸活性。同时,评估了药剂复配及其与辣根素精油联用对嗜卷书虱的协同毒力。结果表明,书虱对马拉硫磷、甲基嘧啶磷、高效氯氰菊酯和溴氰菊酯均表现出较高敏感性,LD_(50)在0.041~0.977μg/cm^(2)之间,可作为敏感基线使用;辣根素精油对书虱的熏蒸活性最高。马拉硫磷和溴氰菊酯复配体积比为4∶5时,增效作用最佳;而甲基嘧啶磷和高效氯氰菊酯配比在2∶1时,增效作用最佳。此外,辣根素与甲基嘧啶磷或高效氯氰菊酯联用均具有增效作用。 展开更多
关键词 仓储害虫 储粮保护剂 熏蒸剂 辣根素
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