期刊文献+
共找到21篇文章
< 1 2 >
每页显示 20 50 100
Comparison between the protective effects of removing circulatory TNF by specific immunoadsorption and by nonspecific Amberlite XAD-7 adsorption on experimental endotoxin shock
1
作者 唐斌 张训 侯凡凡 《Journal of Medical Colleges of PLA(China)》 CAS 1997年第4期298-300,共3页
Objective: To compare the protective effects of removingcirculatory TNF by specific immunoadsorption and by nonspeciflc Amberlite XAD-7 adsorption on experimental endotoxin shock. Methods: New Zealand white rabbits re... Objective: To compare the protective effects of removingcirculatory TNF by specific immunoadsorption and by nonspeciflc Amberlite XAD-7 adsorption on experimental endotoxin shock. Methods: New Zealand white rabbits receiving a lethal dose of endotoxin underwent hemoperfusion through immunoadsorbent or Amberlite BAD-7. Plasma TNF levels. efficiency of the adsorbents and the survival rate were observed. Results: After 2 h of hemopcrfusion through immunoadsorhent or Amberlite XAD-7. plasma TNF levels were significantly lower than those in the control group(P<0.01). and the best result was shown by immunoadsorption (P<0. 01). The 12 h and 18 h survival rates were 70% and 30% in the immunoadsorption group, and 30% and 5% in the Amberlite XAD-7 group respectively (P<0. 05). Conclusion: Compared with the nonspecific Amberlite XAD-7 adsorption,the specific imrnunoadsorption might be a more effective method of removing circulatory TNF and improve the survival rate in endotoxin shock. 展开更多
关键词 ENDOTOXIN shock TUMOR NECROSIS factor IMMUNOADSORPTION amberlite xad-7
下载PDF
杯[4]芳烃修饰Amberlite XAD-4树脂去除水中双氯芬酸 被引量:13
2
作者 王月 熊振湖 周建国 《中国环境科学》 EI CAS CSCD 北大核心 2012年第1期81-88,共8页
通过偶氮化反应将合成的去叔丁基杯[4]芳烃连接到Amberlite XAD-4树脂上,并且采用FTIR、SEM和TG/DTA法表征了杯[4]芳烃修饰Amberlite XAD-4树脂的结构.结果表明,水溶液中杯[4]芳烃修饰Amberlite XAD-4树脂对双氯芬酸的去除率远大于单独A... 通过偶氮化反应将合成的去叔丁基杯[4]芳烃连接到Amberlite XAD-4树脂上,并且采用FTIR、SEM和TG/DTA法表征了杯[4]芳烃修饰Amberlite XAD-4树脂的结构.结果表明,水溶液中杯[4]芳烃修饰Amberlite XAD-4树脂对双氯芬酸的去除率远大于单独AmberliteXAD-4树脂和杯[4]芳烃.双氯芬酸的浓度为20mg/L时,随着杯[4]芳烃修饰AmberliteXAD-4树脂投加量的增加,双氯芬酸的去除率增加很快.当吸附剂量增加到80mg/L时,双氯芬酸的去除率为92.8%,并且达到吸附平衡,吸附的双氯芬酸量为34.02mg/g.Langmuir和Freundlich等温线与实验数据均有很好的拟合度.对热力学参数的计算表明,△H与△G负值显示出反应的放热和自发过程. 展开更多
关键词 杯[4]芳烃 amberlite xad-4树脂 修饰 双氯芬酸 吸附
下载PDF
Amberlite XAD-2000树脂固相萃取富集-火焰原子吸收法测定水中痕量铅 被引量:14
3
作者 程永华 李青彬 《分析试验室》 CAS CSCD 北大核心 2008年第10期106-109,共4页
用Amberlite XAD-2000树脂键合8-羟基喹啉固相萃取富集水中痕量铅,然后用火焰原子吸收法测定。实验了pH,样品处理速度,洗脱剂的类型、用量、浓度、处理速度等因素对铅回收率的影响。在最优条件下,树脂对铅的最大吸附量7.2 mg Pb/g树脂,... 用Amberlite XAD-2000树脂键合8-羟基喹啉固相萃取富集水中痕量铅,然后用火焰原子吸收法测定。实验了pH,样品处理速度,洗脱剂的类型、用量、浓度、处理速度等因素对铅回收率的影响。在最优条件下,树脂对铅的最大吸附量7.2 mg Pb/g树脂,回收率92%;且该方法抗干扰能力强,可用于环境水样检测和干扰离子存在下(如海水中)痕量铅的检测。 展开更多
关键词 痕量 amberlite xad-2000 固相萃取 火焰原子吸收
下载PDF
Amberlite XAD-2000树脂固相萃取富集-火焰原子吸收法测定水中痕量铜 被引量:5
4
作者 程永华 李青彬 《化学试剂》 CAS CSCD 北大核心 2010年第8期718-720,724,共4页
用8-羟基喹啉螯合Amberlite XAD-2000树脂固相萃取富集水中痕量铜,然后用火焰原子吸收法测定其含量。实验了pH、样品处理速度、洗脱剂的类型、用量、浓度、处理速度等因素对铜回收率的影响。在最优条件下,树脂最大吸附量9.35mg铜/g树脂... 用8-羟基喹啉螯合Amberlite XAD-2000树脂固相萃取富集水中痕量铜,然后用火焰原子吸收法测定其含量。实验了pH、样品处理速度、洗脱剂的类型、用量、浓度、处理速度等因素对铜回收率的影响。在最优条件下,树脂最大吸附量9.35mg铜/g树脂,回收率96%。实验表明,该方法可用于环境水样检测和干扰离子存在下(如海水中)痕量铜的检测。 展开更多
关键词 痕量 amberlite xad-2000 固相萃取 火焰原子吸收
下载PDF
Amberlite XAD-1600吸附树脂精制白芍活性成分的实验研究
5
作者 吴志华 刘中秋 +1 位作者 范艳芳 张嘉杰 《中药材》 CAS CSCD 北大核心 2009年第5期797-799,共3页
目的:探讨白芍乙醇提取物中主要活性成分的提取、分离与精制的方法。方法:采用多聚体吸附树脂Amberlite XAD-1600,分离精制75%乙醇提取物。以芍药苷的含量和抗炎作用为评价指标,比较评价乙醇提取物经XAD-1600处理前后的化学和生物等效... 目的:探讨白芍乙醇提取物中主要活性成分的提取、分离与精制的方法。方法:采用多聚体吸附树脂Amberlite XAD-1600,分离精制75%乙醇提取物。以芍药苷的含量和抗炎作用为评价指标,比较评价乙醇提取物经XAD-1600处理前后的化学和生物等效性。结果:XAD-1600除去乙醇提取物中杂质的程度达62.75%,同时能有效保留芍药苷,其保留率为98.80%。结论:XAD-1600树脂可有效地分离与精制白芍活性成分。 展开更多
关键词 amberlite xad-1600吸附树脂 芍药苷 精制 抗炎
原文传递
Octa-<i>O</i>-Methoxy Resorcin [4] Arene Amberlite XAD-4 Polymeric Chelating Resin for Solid Phase Extraction, Preconcentration, Separation and Trace Determination of Ni(II), Cu(II), Zn(II) and Cd(II) Ions
6
作者 Disha J. Vyas Bharat A. Makwana +1 位作者 Keyur D. Bhatt Vinod K. Jain 《American Journal of Analytical Chemistry》 2013年第5期238-251,共14页
Synthetic resin, Amberlite XAD-4 was linked covalently with the third generation supramolecule, octa-O-methoxy resorcin [4] arene through -N=N-group to form chelating resin, which has been characterized and effectivel... Synthetic resin, Amberlite XAD-4 was linked covalently with the third generation supramolecule, octa-O-methoxy resorcin [4] arene through -N=N-group to form chelating resin, which has been characterized and effectively used for the separation and preconcentration of metal ions such as Ni(II), Cu(II), Zn(II) and Cd(II). Critical parameters such as pH, flow rate, sorption capacity, breakthrough studies, distribution coefficient, preconcentration factor, concentration of eluting agents responsible for quantitative extraction of metal ions were optimized. The synthesized resin showed good binding affinity towards Ni(II), Cu(II), Zn(II) and Cd(II) under selective pH conditions. Good breakthrough capacity and fast exchange kinetics of the resin lead to effective separation of metal ions from their binary and ternary mixture by column method on the basis of pH and eluting agents. The resin could be reused for about 8 -10 cycles. The proposed method having the analytical data with the relative standard deviation (RSD) 2% and with recoveries of analytes higher than 98%, reflects upon the reproducibility and reliability of the method which has been successfully applied in the separation and determination of Ni(II), Cu(II), Zn(II) and Cd(II) ions in synthetic, natural and ground water samples. 展开更多
关键词 SEPARATION PRECONCENTRATION amberlite xad-4 Octa--O-Methoxy Resorcin [4] ARENE Solid Phase Extraction
下载PDF
ADSORPTION STUDIES OF TANNIC ACID BY COMMERCIAL ESTER RESIN XAD-7 被引量:4
7
作者 李爱民 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2010年第2期231-239,共9页
Tannic acid and its related compounds are known as refractory organic pollutants, and it can create serious problems for the environment. The adsorption and desorption studies of tannic acid on commercial resins XAD-7... Tannic acid and its related compounds are known as refractory organic pollutants, and it can create serious problems for the environment. The adsorption and desorption studies of tannic acid on commercial resins XAD-7 and D-201 are performed, and all data indicates resin XAD-7 can be used as an effective adsorbent for removing tannic acid during water/wastewater treatment. Furthermore, adsorption thermodynamics studies indicate different adsorption mechanisms for TA on XAD-7 and D-201. FT-IR and solid state 13C-NMR spectroscopy are used to explain the adsorption force between XAD-7 and TA. It suggests that hydrogen bonding is the main adsorption force for TA. Finally, XAD-7's adsorption capacity in the presence of different metal ions is investigated, which indicates that heavy metal ions in solutions can decrease the adsorption capacity for TA on ester resin XAD-7. 展开更多
关键词 ADSORPTION Tannic acid RESIN xad-7 Metal ion.
下载PDF
XAD-7HP树脂纯化款冬花多糖工艺研究 被引量:1
8
作者 韩毅丽 张澍 裴晓丽 《广州化工》 CAS 2022年第12期60-62,共3页
研究XAD-7HP型大孔吸附树脂纯化款冬粗多糖的影响因素。采用单因素分析法考察了影响款冬花多糖纯化效果的主要因素款冬花多糖的上样浓度、上样速度、乙醇洗脱液浓度、洗脱速度四个因素。结果表明款冬花多糖的最佳纯化工艺为:0.3208 mg/m... 研究XAD-7HP型大孔吸附树脂纯化款冬粗多糖的影响因素。采用单因素分析法考察了影响款冬花多糖纯化效果的主要因素款冬花多糖的上样浓度、上样速度、乙醇洗脱液浓度、洗脱速度四个因素。结果表明款冬花多糖的最佳纯化工艺为:0.3208 mg/mL的款冬花多糖液以1.0 mL/min的速度上样,采用65%的乙醇溶液以1.0 mL/min的流速进行洗脱。经XAD-7HP树脂纯化后,款冬花多糖的纯度达到了72.98%,纯化效果显著。 展开更多
关键词 款冬花 多糖 xad-7HP大孔吸附树脂 纯化
下载PDF
Solid-liquid extraction for yttrium recovery using porous polymeric resin(XAD-7) functionalized with D_(2)EHPA
9
作者 P.A.Martínez-Montoya R.G.Sanchez-Alvarado +3 位作者 D.Y.Medina-Velazquez M.L.Carrera-Jota P.Garnica-Chávez A.de J.Morales-Ramirez 《Journal of Rare Earths》 SCIE EI CAS CSCD 2024年第9期1764-1773,I0005,共11页
The recovery of yttrium is proposed by applying a solid-liquid extraction process using di-2-ethyl hexylphosphoric acid(D2EHPA) as extracting agent.The extracting agents were supported on a macro porous polymeric resi... The recovery of yttrium is proposed by applying a solid-liquid extraction process using di-2-ethyl hexylphosphoric acid(D2EHPA) as extracting agent.The extracting agents were supported on a macro porous polymeric resin XAD-7(solid phase).Yttrium ions extraction and discharge tests were performed,firstly from a synthetic aqueous solution of 100 mg/L Y(liquid phase) at 25℃ with stirring.The effects of pH of aqueous solutions bearing yttrium,volume fraction of extracting agents and the solid/liquid(S/L)ratio on the yttrium recovery were studied.The most favorable conditions for yttrium ions extraction are;20 vol% D_(2)EHPA functionalized resin,pH=1.5 and an S/L ratio of 10 mg/mL The discharge of yttrium ions was done under the same conditions of extraction stage,using a 2 mol/L [H_(2)SO_(4)] as stripping solution.Up to 80% yttrium is extracted,while 75% yttrium is recovered in the striping solution.In all experiments,the reaction equilibrium is reached after 20 min,and the kinetics for the extraction stage was determined as a second-order model.Also,experiments were carried out to discharge the yttriumloaded resins,and it has been determined that the best pH value to strip the Y ions is 1.5.Cyclic tests of extraction and discharge for yttrium ions show that the functionalized resin can work at least five cycles without decreasing its efficiency.Finally,the proposed process was tested in a real solution with Y ions fro m a waste fluorescent la mp powder leached in H_(2)SO_(4),demonstrating the ability to effectively recover yttrium,separating it from various metals from the studied residue. 展开更多
关键词 Yttrium recovery Rare earth solid-liquid extraction xad-7 resin Fluorescent lamps recycling Solid-liquid kinetics extraction
原文传递
Determination of Some Trace Metals in Environmental Samples by Flame AAS Following Solid Phase Extraction with Amberlite XAD-2000 Resin after Complexing with 8-Hydroxyquinoline 被引量:2
10
作者 DURAN, Celal SENTURK, H. Basri +5 位作者 GUNDOGDU, Ali BULUT, V. Numan EICi, Latif SOYLAK, Mustafa TUFEKCI, Mehmet UYGUR, Yaprak 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2007年第2期196-202,共7页
A procedure for preconcentration of Mn(Ⅱ), Fe(Ⅱ), Co(H), Cu(Ⅱ), Cd(Ⅱ), Zn(Ⅱ), Pb(Ⅱ) and Ni(Ⅱ) based on retention of their complexes with 8-hydroxyquinoline (HQ) on Amberlite XAD-2000 resin in ... A procedure for preconcentration of Mn(Ⅱ), Fe(Ⅱ), Co(H), Cu(Ⅱ), Cd(Ⅱ), Zn(Ⅱ), Pb(Ⅱ) and Ni(Ⅱ) based on retention of their complexes with 8-hydroxyquinoline (HQ) on Amberlite XAD-2000 resin in a column was proposed for the analysis of environmental samples by flame AAS. Various parameters such as pH, eluent type, volume, concentration, flow rate and volume of sample solution, and matrix interference effect on the retention of the metal ions were investigated. The optimum pHs for the retention of metal complexes in question were about 6 except for Mn^2+ for whose value is 8. The loading capacity of the adsorbent for these metals and their recoveries from the resin under the optimum conditions were in the range 6.82-9.26 mg·g^-1 and 95%-101%, respectively. The enrichment factor was calculated as 100 and the limit of detection was in the range 0.3-2.2 μg·L^-1 (n=20, blank+ 3s). The proposed enrichment method was applied to tap water, stream water and vegetable samples. The validation of the procedure was carried out by analysis of certified reference material and standard addition. The analytes were determined with a relative standard deviation lower than 6% in all samples. 展开更多
关键词 water and vegetable sample trace metal PRECONCENTRATION amberlite xad-2000 FAAS 8-HYDROXYQUINOLINE
原文传递
Kinetics and thermodynamics modeling of Nd(Ⅲ) removal from aqueous solution using modified Amberlite XAD7
11
作者 Petru Negrea Andreea Gabor +3 位作者 Corneliu Mircea Davidescu Mihaela Ciopec Adina Negrea Narcis Duteanu 《Journal of Rare Earths》 SCIE EI CAS CSCD 2020年第3期306-314,共9页
New adso rbent material was obtained by modification of commercial Amberlite XAD7 with thiourea that represents a non-toxic,cheap and environmentally friendly extractant.Prepared adsorbent was used for removal of neod... New adso rbent material was obtained by modification of commercial Amberlite XAD7 with thiourea that represents a non-toxic,cheap and environmentally friendly extractant.Prepared adsorbent was used for removal of neodymium ions from aqueous solutions.Thiourea modified Amberlite involved in this study shows good adsorption capacities(74.3 mg/g) and excellent efficiency during Nd removal process.In order to elucidate the mechanism of the Nd adsorption process kinetic,thermodynamic and equilibrium studies were performed,establishing this way which kinetic model better describes the Nd adsorption process.Moreover the thermodynamic studies prove that the Nd adsorption on thiourea modified Amberlite XAD7 is an endothermic and spontaneous process. 展开更多
关键词 NEODYMIUM amberlite XAD7 ADSORPTION mechanism ADSORPTION ISOTHERM THIOUREA Rare earths
原文传递
工作场所空气中邻仲丁基苯酚测定方法的研究 被引量:5
12
作者 李小娟 仓公敖 +1 位作者 马永建 吉文亮 《中国职业医学》 CAS 北大核心 2008年第6期510-511,514,共3页
目的建立工作场所空气中邻仲丁基苯酚的溶剂解吸毛细管柱气相色谱测定方法。方法XAD-7管吸附空气中邻仲丁基苯酚,用甲醇解吸,以二甲基甲酰胺作内标,毛细管柱气相色谱(FID)测定。结果空气中邻仲丁基苯酚的浓度在0~222mg/m3(以采集4.5 L... 目的建立工作场所空气中邻仲丁基苯酚的溶剂解吸毛细管柱气相色谱测定方法。方法XAD-7管吸附空气中邻仲丁基苯酚,用甲醇解吸,以二甲基甲酰胺作内标,毛细管柱气相色谱(FID)测定。结果空气中邻仲丁基苯酚的浓度在0~222mg/m3(以采集4.5 L空气计,相当于邻仲丁基苯酚标准溶液浓度在0~1 000 mg/L)时,线性方程为:y=0.004x+0.004,r=0.999 8。检出限为1.0 mg/L,最低检出浓度为0.2 mg/m3(以采集4.5 L空气计算);解吸效率≥95.6%;穿透容量(BTV)>6.6 mg;采样效率为100%;样品在室温下可保存7 d。结论本方法适用于工作场所空气中邻仲丁基苯酚的浓度测定。 展开更多
关键词 邻仲丁基苯酚 xad-7 毛细管气相色谱 检测方法
下载PDF
微型柱现场在线采样技术测定海水中痕量铜 被引量:3
13
作者 吴冬梅 王爱霞 郭黎平 《东北师大学报(自然科学版)》 CAS CSCD 北大核心 2006年第1期80-83,共4页
在海水(pH7.8-8.5)条件下,使用微型柱现场在线采样(MFS)技术直接对海水中痕量铜进行预富集,然后在实验室中采用流动注射-火焰原子吸收(FI—FAAS)联用系统成功地实现了对海水中铜的测定.当采样体积为50mL时,其富集因子为432... 在海水(pH7.8-8.5)条件下,使用微型柱现场在线采样(MFS)技术直接对海水中痕量铜进行预富集,然后在实验室中采用流动注射-火焰原子吸收(FI—FAAS)联用系统成功地实现了对海水中铜的测定.当采样体积为50mL时,其富集因子为432,检出限为0.019μg/L,相对标准偏差为1.68%.对海水中微量元素标准物质GBW(E)080040和大连老虎滩区域海水中的(沁进行分析,均得到满意结果. 展开更多
关键词 微型柱现场在线采样技术 amberlite xad-2键合双硫腙 流动注射-火焰原子吸收 海水
下载PDF
葡萄皮花色苷的树脂纯化及稳定性研究 被引量:9
14
作者 李杨 韩业慧 +1 位作者 李记明 徐岩 《酿酒科技》 北大核心 2012年第1期28-31,共4页
以烟73酿酒葡萄为花色苷提取材料,采用HPLC分析方法,对10种树脂的吸附/解析效果进行了比较。选用性能较优的XAD-7HP树脂进行纯化实验并对纯化后花色苷的稳定性进行了研究。结果表明,纯化处理使花色苷在干物质中的含量由处理前的48.16%... 以烟73酿酒葡萄为花色苷提取材料,采用HPLC分析方法,对10种树脂的吸附/解析效果进行了比较。选用性能较优的XAD-7HP树脂进行纯化实验并对纯化后花色苷的稳定性进行了研究。结果表明,纯化处理使花色苷在干物质中的含量由处理前的48.16%提高到处理后的94.93%,色价由处理前的47.7提高到处理后的291.6,回收率为85.7%。花色苷的稳定性实验结果表明,随着光照强度、温度、pH值的升高,花色苷的不稳定性明显加剧。在避光、pH值为3,4℃下存放7 d,花色苷的保留率在95%以上,适宜于花色苷的短期保存。 展开更多
关键词 花色苷 纯化方法 稳定性 xad-7HP 葡萄皮
下载PDF
基于组合色谱技术的蓝莓果实中花色苷元制备 被引量:5
15
作者 王二雷 陈晶晶 +1 位作者 刘彦君 刘静波 《食品科学》 EI CAS CSCD 北大核心 2018年第18期227-234,共8页
首先采用液液萃取及大孔树脂层析技术研究花色苷的制备方法,萃取剂为乙酸乙脂,大孔树脂为Amberlite XAD-7HP,在此基础上制得花色苷粗提液。花色苷经酸水解、浓缩后,采用固相萃取技术(C_(18)小柱)对花色苷元进行除酸、除糖处理,制得了纯... 首先采用液液萃取及大孔树脂层析技术研究花色苷的制备方法,萃取剂为乙酸乙脂,大孔树脂为Amberlite XAD-7HP,在此基础上制得花色苷粗提液。花色苷经酸水解、浓缩后,采用固相萃取技术(C_(18)小柱)对花色苷元进行除酸、除糖处理,制得了纯度为70.2%的花色苷元混合物。最后采用半制备型高效液相色谱技术实现4种主要花色苷元单体的分离,分别为飞燕草素(纯度98.2%)、矢车菊素(纯度96.3%)、牵牛花素(纯度92.6%)、锦葵色素(纯度90.5%)。本研究可为花色苷元的规模化制备及后期功能活性研究提供技术参考和依据。 展开更多
关键词 花色苷元 amberlite xad-7HP大孔树脂 酸水解 固相萃取 高效液相色谱
下载PDF
Recovery of Heavy Metal Using Solvent Impregnated Resin (SIR) Coupled with Donnan Dialysis
16
作者 Dalila Berdous Djamel Eddine Akretche 《Materials Sciences and Applications》 2012年第10期704-712,共9页
A membrane process for metal recovery from aqueous solutions was studied. Metal ions diffused from the feed compartment to the stripping compartment through an hybrid Donnan dialysis which consists to combine two ion ... A membrane process for metal recovery from aqueous solutions was studied. Metal ions diffused from the feed compartment to the stripping compartment through an hybrid Donnan dialysis which consists to combine two ion exchange membranes with solvent impregnated resin (SIR). The aim of this work is to study the recovery of Pb(II), Ag(I) and Cu(II) from nitrate solutions by using SIR combined with classical Donnan dialysis. The resin has been prepared by impregnating the Amberlite XAD-4 using three different extractants namely: di-2-ethyl hexyl phosphine acide (D2EHPA), tris-octyl phosphine oxide (TOPO) and Diphenylthiourea (DPT). Experiments were performed as a function of nature of the extractant impregnated on the XAD-4 resin as well as the concentration in the resin phase, the stripping pH, the concentration ratio of metal ions in the feed compartment and the nature of the counter ions in strip compartment. The results show that the D2EHPA is the better extractant for the metal ions used in this work, and it’s shown a good selectivity for the separation between ions. 展开更多
关键词 Donnan DIALYSIS Hybrid Process amberlite xad-4 SOLVENT IMPREGNATED Resin D2EHPA TOPO Dipehenylthiourea
下载PDF
用分光光度计检测的反相高效液相色谱同步测定从涂有甲基乙二醛双(4-苯基-3-缩氨基硫脲)的树脂上收集和洗脱的Pt(Ⅱ)和Pd(Ⅱ)
17
作者 唐良保 《国外铀金地质》 1999年第2期182-186,共5页
制备了用二甲基乙二醛双(4-苯基-3-缩氨基硫脲)(DMBS)涂渍的大网状树脂(AmberliteXAD-7),应用于水溶液中Pt(Ⅱ)和Pd(Ⅱ)的预富集。用该方法在存有碘离子的酸性溶液中从涂层树脂(DMBS-XAD-7)上定量地收集Rt(Ⅱ)和Pd(Ⅱ)。作为与DMBS形成... 制备了用二甲基乙二醛双(4-苯基-3-缩氨基硫脲)(DMBS)涂渍的大网状树脂(AmberliteXAD-7),应用于水溶液中Pt(Ⅱ)和Pd(Ⅱ)的预富集。用该方法在存有碘离子的酸性溶液中从涂层树脂(DMBS-XAD-7)上定量地收集Rt(Ⅱ)和Pd(Ⅱ)。作为与DMBS形成整合物的金属离子用少量的N,N-二甲基酰胺很容易从DMBS-XAD-7中洗脱。这种收集和洗脱的方法被用于Pt(Ⅱ)和Pd(Ⅱ)的同步测定,该测定采用分光光度计检测,用十八基硅烷为色层柱,以丙酮-水作流动相的反相高效液相色谱来完成的。推荐的方法已用于市场上可买到的样品中铂和钯的测定。 展开更多
关键词 DMBS xad-7树脂 反相 高效液相色谱
下载PDF
联合调控对中国红豆杉细胞关键酶基因表达的影响 被引量:4
18
作者 高明波 张卫 +2 位作者 李兴泰 阮成江 范圣第 《中国生物工程杂志》 CAS CSCD 北大核心 2010年第8期31-36,共6页
红豆杉悬浮培养细胞可以持续提供抗癌药物紫杉醇及一些紫杉烷类。在中国红豆杉悬浮培养细胞中,云南紫杉烷C(Tc)是主要的紫杉烷。为了更理性地调控紫杉醇或有用紫杉烷的生产,有必要深入了解其生物合成过程。采用实时定量PCR(Real-time Qu... 红豆杉悬浮培养细胞可以持续提供抗癌药物紫杉醇及一些紫杉烷类。在中国红豆杉悬浮培养细胞中,云南紫杉烷C(Tc)是主要的紫杉烷。为了更理性地调控紫杉醇或有用紫杉烷的生产,有必要深入了解其生物合成过程。采用实时定量PCR(Real-time Quantitative PCR,即RQPCR)技术考察经调控后紫杉醇及紫杉烷代谢中关键酶基因—TASY,T5αH,TDAT,T10βH,TαH,T14βH表达水平的变化。在细胞培养的第7天和12天,分别以100μmol/L2,3-二羟丙基茉莉酸(DHPJA)诱导,同时在细胞培养第7天进行20g/L蔗糖饲喂、100g/LXAD-7HP的原位吸附。该联合调控处理使得细胞培养第30天时,Tc产量高达1517±37mg/L,是对照处理的11.1倍,是DHPJA重复诱导联合蔗糖饲喂处理的1.7倍。RQ-PCR结果显示:DHPJA的加入可使6个基因表达水平显著提高,但在12小时后快速下降,需补充DHPJA以再次提高基因表达水平。吸附剂同时引入会延缓基因表达水平的提高速度,但却能维持基因表达处于一个较高的水平,表现为在细胞培养中后期,基因表达水平将显著高于无吸附剂的调控体系。与13α-羟化相对应的TαH基因有所不同,吸附剂的存在更显著地抑制其表达,但仍有维持表达的功能。 展开更多
关键词 中国红豆杉 云南紫杉烷C 2 3-二羟丙基茉莉酸 xad-7HP 实时定量PCR
原文传递
原位吸附和茉莉酸甲酯联合使用显著提高中国红豆杉细胞云南紫杉烷c的生产 被引量:3
19
作者 高明波 张卫 虞星炬 《生物工程学报》 CAS CSCD 北大核心 2010年第2期223-229,共7页
本实验所用的中国红豆杉细胞悬浮培养体系中,云南紫杉烷c(Tc)是主要的次生代谢产物,该化合物有类神经生长因子活性,提高其产量是进一步规模化生产的前提。本研究考察了原位吸附和茉莉酸甲酯(MJA)联合调控提高Tc产量的可能性。在培养的第... 本实验所用的中国红豆杉细胞悬浮培养体系中,云南紫杉烷c(Tc)是主要的次生代谢产物,该化合物有类神经生长因子活性,提高其产量是进一步规模化生产的前提。本研究考察了原位吸附和茉莉酸甲酯(MJA)联合调控提高Tc产量的可能性。在培养的第7天加入浓度为100μmol/L的MJA虽然会使细胞的生物量下降10%~30%,但是单位细胞内Tc含量和Tc产量均有显著提高,分别是对照的3.6和3.3倍。吸附剂XAD-7在不同时间加入对Tc的合成影响显著。在培养的第7天同时加入100μmol/L的MJA和100g/L的XAD-7会使细胞生物量增加,Tc产量显著提高。培养到第21天,Tc产量达477.4mg/L,为对照的6.3倍,为只加MJA的1.9倍,其中94%的Tc被树脂吸附。实验结果表明,在MJA诱导高表达的过程中,吸附剂XAD-7的加入使细胞内代谢产物外泌,浓度降低,减轻产物反馈抑制现象,从而大幅度提高代谢物产量,有较好的生产前景。 展开更多
关键词 中国红豆杉 细胞悬浮培养 茉莉酸甲酯 xad-7 云南紫杉烷c
原文传递
青稞麸皮β-葡聚糖脱色工艺研究 被引量:3
20
作者 贾莹 常雅宁 +1 位作者 俞建瑛 何彦康 《食品工业》 北大核心 2013年第8期107-110,共4页
研究了4种树脂对青稞β-葡聚糖粗品溶液脱色效果的影响,在静态吸附试验的基础上,筛选出D201和XAD-7两种树脂进行动态试验研究。研究发现,D201树脂的最佳脱色工艺为:样品溶液温度40℃,pH 5,流速0.5mL/min,该条件下青稞麸皮β-葡聚糖的脱... 研究了4种树脂对青稞β-葡聚糖粗品溶液脱色效果的影响,在静态吸附试验的基础上,筛选出D201和XAD-7两种树脂进行动态试验研究。研究发现,D201树脂的最佳脱色工艺为:样品溶液温度40℃,pH 5,流速0.5mL/min,该条件下青稞麸皮β-葡聚糖的脱色率为67.8%,β-葡聚糖损失率约25%;XAD-7树脂的最佳脱色工艺为:样品溶液温度40℃,pH 6,流速0.5 mL/min,该条件下青稞麸皮β-葡聚糖的脱色率可达72.9%,β-葡聚糖损失率为4.3%。XAD-7脱色性能较好,但成本高;D201树脂脱色性能略差,但成本低。两种树脂吸附后都可再生,且再生性能良好。 展开更多
关键词 Β-葡聚糖 青稞 脱色 D201 xad-7
原文传递
上一页 1 2 下一页 到第
使用帮助 返回顶部