Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F（ab’）<sub>2</sub>were labelled with[<sup>131</sup>I]according to chloramine T method.The labelling rate andradioactivity were 51...Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F（ab’）<sub>2</sub>were labelled with[<sup>131</sup>I]according to chloramine T method.The labelling rate andradioactivity were 51% and 9.58×10<sup>4</sup>Bq/mg,respectively.After administration of the[<sup>131</sup>I]-labelled conjugates through mouse tail vein,hepatoma xenografts were clearly im-aged,Localization index was 2.15,3.09,and 6.99 for HAb8,HAb18 IgG and HAb18F（ab’）<sub>2</sub>,respectively.A total of 16 hepatoma-bearing nude mice（SMMU-LTNM）were di-vided into 4 groups.When xenografts reached 0.3cm in diameter,the treatment was be-gun by injection of the[<sup>131</sup>I]-conjugates through mouse tail vein.After 2 weeks of treat-ment,obvious inhibitory or killing effects on the xenografts were found.The tu-mor-bearing mice were killed during the period from 14 to 21d after treatment.Xenografts as well as other organs were sampled and viewed under light microscope ac-cording to routine procedure.The vital organs,such as heart,liver,spleen,lung,kidneyand brain showed no pathological lesions.In hepatoma xenografts,some tumor cellmembranes were found,with obscure cytoplasmic structure and increased eosinophilia,representing necrotic alterations.展开更多
AIM To explore relationships between human carcinomas and mycoplasma infection.METHODS Monoclonal antibody PD4, which specifically recognizes a distinct protein from mycoplasma hyorhinis, was used to detect mycoplasma...AIM To explore relationships between human carcinomas and mycoplasma infection.METHODS Monoclonal antibody PD4, which specifically recognizes a distinct protein from mycoplasma hyorhinis, was used to detect mycoplasma infection in different paraffinembedded carcinoma tissues with immunohistochemistry. PCR was applied to amplify the mycoplasma DNA from the positive samples for confirming immunohistochemistry.RESULTS Fifty of 90 cases (56%) of gastric carcinoma were positive for mycoplasma hyorhinis. In other gastric diseases, the mycoplasma infection ratio was 28% (18/49) in chronic superficial gastritis, 30% (14/ 46) in gastric ulcer and 37% (18/ 49) in intestinal metaplasia. The difference is significant with gastric cancer (X2=12.06, P＜0.05). In colon carcinoma, the mycoplasma infection ratio was 55.1% (32/58), but it was 20.9% (10/49) in adenomarous polyp (X2=13.46, P＜0.005).Gastric and colon cancers with high differentiation had a higher mycoplasma infection ratio than those with low differentiation (P＜ 0.05). Mycoplasma infection in esophageal cancer, lung cancer, breast cancer and glioma was 50.9% (27/53), 52.6% (31/ 59), 39.7%(25/63) and 41% (38/91), respectively. The mycoplasma DNA was successfully amplified with the DNA extracted from the cancer tissues that were positive for mycoplasma infection (detected with antibody PD4).CONCLUSION There was high correlation between mycoplasma infection and different cancers, which suggests the possibility of an association between the two. The mechanism involved in oncogenesis by mycoplasma remains unknown.展开更多
In this report,a comparative study is made of the function test of spontaneousT suppressor cell（STs）and T Lymphocyte subsets in patients with epidemic hemorrha-gic fever（EHF）.It was found that in the early stages ...In this report,a comparative study is made of the function test of spontaneousT suppressor cell（STs）and T Lymphocyte subsets in patients with epidemic hemorrha-gic fever（EHF）.It was found that in the early stages of the disease the activity of STs wasmarkedly lower than that of the controls,while the percentage of CD<sub>?</sub><sup>+</sup> cells increasedsignificantly,which led to the decrease and reciprocation of CD<sub>4</sub>/CD<sub>8</sub> ratio,and that theactivity of STs was reversely related to the proportion of CD<sub>8</sub><sup>+</sup> cells on linear regressionanalysis,indicating that the CD<sub>8</sub><sup>+</sup> cells increased may mainly belong to cytotoxic T cells.It was also shown that the changes of STs function and CD<sub>4</sub>/CD<sub>8</sub> ratio were related tothe abnormalities of serum C<sub>3</sub> content and circulating immune complex.The results sug-gest that the disturbance of host cellular immunoregulation may play an important rolein the pathogenesis of EHF.展开更多
By using anti-ras p21 mouse monoclonal antibody(McAb),SCI-oncogema 1,the au-thors examined immunohistochemical staining in pancreatic adenocarcinoma.The percentage ofpositive staining was 55.8%(24/43)and was related t...By using anti-ras p21 mouse monoclonal antibody(McAb),SCI-oncogema 1,the au-thors examined immunohistochemical staining in pancreatic adenocarcinoma.The percentage ofpositive staining was 55.8%(24/43)and was related to either histopathological grade or clinicalstage.Upon statistical analysis of the correlation between the positive staining of anti-ras p21and patient prognosis with Klaplan-Meier curve and Log-rant test,the positive staining casesshowed comparatively better prognosis than the negative ones,suggesting that ras p21 expres-sion may be important in the early stage of pancreatic carcinoma.展开更多
Doxorubicin （DXR） was entrapped in liposomes coated with themonoclonal antibody,MGb<sub>2</sub>,against human gastric cancer.Negative-stain electronmicroscopy showed the diameter of liposomes was 105.6&#...Doxorubicin （DXR） was entrapped in liposomes coated with themonoclonal antibody,MGb<sub>2</sub>,against human gastric cancer.Negative-stain electronmicroscopy showed the diameter of liposomes was 105.6±21.1nm.The drugentrapment efficiency was 47.6±8.1%.There were about 5667 drug moleculesentrapped and 48 antibody molecules incorporated per liposome.It was confirmedby ELISA and binding assay that the immunoliposomes could bind selectively andcarry their contents to human gastric cancer cell line,BGC-823.Theimmunoliposomes showed specific cytotoxicity to BGC-823,2.3-fold more effectivethan free DXR and 56.7-fold more effective than non-specific liposomes,but only1/25 of toxicity to normal human embryonic lung cell line,SL<sub>7</sub>,as comparedwith free DXR.This study indicates that the immunoliposomes could deliverDXR selectively to the target cells and might be useful in targeting chemotherapyof human gastric cancer.展开更多
文摘Anti-hepatoma monoclonal antibodies HAb8,HAb18 IgG and HAb18 F（ab’）<sub>2</sub>were labelled with[<sup>131</sup>I]according to chloramine T method.The labelling rate andradioactivity were 51% and 9.58×10<sup>4</sup>Bq/mg,respectively.After administration of the[<sup>131</sup>I]-labelled conjugates through mouse tail vein,hepatoma xenografts were clearly im-aged,Localization index was 2.15,3.09,and 6.99 for HAb8,HAb18 IgG and HAb18F（ab’）<sub>2</sub>,respectively.A total of 16 hepatoma-bearing nude mice（SMMU-LTNM）were di-vided into 4 groups.When xenografts reached 0.3cm in diameter,the treatment was be-gun by injection of the[<sup>131</sup>I]-conjugates through mouse tail vein.After 2 weeks of treat-ment,obvious inhibitory or killing effects on the xenografts were found.The tu-mor-bearing mice were killed during the period from 14 to 21d after treatment.Xenografts as well as other organs were sampled and viewed under light microscope ac-cording to routine procedure.The vital organs,such as heart,liver,spleen,lung,kidneyand brain showed no pathological lesions.In hepatoma xenografts,some tumor cellmembranes were found,with obscure cytoplasmic structure and increased eosinophilia,representing necrotic alterations.
基金Supported by National 863 Project (102-10-01-08)National Natural Science Foundation of China(39570405)+1 种基金Natural Science Foundation of Beijing(7941001)State Key Basic Research Program(G1998051203)
文摘AIM To explore relationships between human carcinomas and mycoplasma infection.METHODS Monoclonal antibody PD4, which specifically recognizes a distinct protein from mycoplasma hyorhinis, was used to detect mycoplasma infection in different paraffinembedded carcinoma tissues with immunohistochemistry. PCR was applied to amplify the mycoplasma DNA from the positive samples for confirming immunohistochemistry.RESULTS Fifty of 90 cases (56%) of gastric carcinoma were positive for mycoplasma hyorhinis. In other gastric diseases, the mycoplasma infection ratio was 28% (18/49) in chronic superficial gastritis, 30% (14/ 46) in gastric ulcer and 37% (18/ 49) in intestinal metaplasia. The difference is significant with gastric cancer (X2=12.06, P＜0.05). In colon carcinoma, the mycoplasma infection ratio was 55.1% (32/58), but it was 20.9% (10/49) in adenomarous polyp (X2=13.46, P＜0.005).Gastric and colon cancers with high differentiation had a higher mycoplasma infection ratio than those with low differentiation (P＜ 0.05). Mycoplasma infection in esophageal cancer, lung cancer, breast cancer and glioma was 50.9% (27/53), 52.6% (31/ 59), 39.7%(25/63) and 41% (38/91), respectively. The mycoplasma DNA was successfully amplified with the DNA extracted from the cancer tissues that were positive for mycoplasma infection (detected with antibody PD4).CONCLUSION There was high correlation between mycoplasma infection and different cancers, which suggests the possibility of an association between the two. The mechanism involved in oncogenesis by mycoplasma remains unknown.
文摘In this report,a comparative study is made of the function test of spontaneousT suppressor cell（STs）and T Lymphocyte subsets in patients with epidemic hemorrha-gic fever（EHF）.It was found that in the early stages of the disease the activity of STs wasmarkedly lower than that of the controls,while the percentage of CD<sub>?</sub><sup>+</sup> cells increasedsignificantly,which led to the decrease and reciprocation of CD<sub>4</sub>/CD<sub>8</sub> ratio,and that theactivity of STs was reversely related to the proportion of CD<sub>8</sub><sup>+</sup> cells on linear regressionanalysis,indicating that the CD<sub>8</sub><sup>+</sup> cells increased may mainly belong to cytotoxic T cells.It was also shown that the changes of STs function and CD<sub>4</sub>/CD<sub>8</sub> ratio were related tothe abnormalities of serum C<sub>3</sub> content and circulating immune complex.The results sug-gest that the disturbance of host cellular immunoregulation may play an important rolein the pathogenesis of EHF.
文摘By using anti-ras p21 mouse monoclonal antibody(McAb),SCI-oncogema 1,the au-thors examined immunohistochemical staining in pancreatic adenocarcinoma.The percentage ofpositive staining was 55.8%(24/43)and was related to either histopathological grade or clinicalstage.Upon statistical analysis of the correlation between the positive staining of anti-ras p21and patient prognosis with Klaplan-Meier curve and Log-rant test,the positive staining casesshowed comparatively better prognosis than the negative ones,suggesting that ras p21 expres-sion may be important in the early stage of pancreatic carcinoma.
文摘Doxorubicin （DXR） was entrapped in liposomes coated with themonoclonal antibody,MGb<sub>2</sub>,against human gastric cancer.Negative-stain electronmicroscopy showed the diameter of liposomes was 105.6±21.1nm.The drugentrapment efficiency was 47.6±8.1%.There were about 5667 drug moleculesentrapped and 48 antibody molecules incorporated per liposome.It was confirmedby ELISA and binding assay that the immunoliposomes could bind selectively andcarry their contents to human gastric cancer cell line,BGC-823.Theimmunoliposomes showed specific cytotoxicity to BGC-823,2.3-fold more effectivethan free DXR and 56.7-fold more effective than non-specific liposomes,but only1/25 of toxicity to normal human embryonic lung cell line,SL<sub>7</sub>,as comparedwith free DXR.This study indicates that the immunoliposomes could deliverDXR selectively to the target cells and might be useful in targeting chemotherapyof human gastric cancer.
