An antithrombus enzyme (ATE) was precipitated by (NH4)2SO4 or ethanol from supernatant of Bacillus subtilis culture broth then purified using ion exchange chromatography on CM-sepharose fast flow. The effects of ionic...An antithrombus enzyme (ATE) was precipitated by (NH4)2SO4 or ethanol from supernatant of Bacillus subtilis culture broth then purified using ion exchange chromatography on CM-sepharose fast flow. The effects of ionic strength and pH value on protein adsorption, the gradient elution at different flow rates and step elution were examined respectively. The recovery yield of the optimised process was 74.5% with a purification factor 8.1. The ATE molecular weight was estimated as 30ku by SDS-PAGE. The experimental results showed that the enzyme was stable in the range of pH 7 to pH11, and temperature 25℃ to 37℃.展开更多
利用磁控溅射技术合成了Ti Ta O薄膜涂层。采用体外血小板粘附试验、动态凝血时间测定以及动物体内试片埋植试验等评价方法 ,对涂层的抗凝血特性进行了研究 ;并采用Tauc法研究了涂层的禁带宽度。研究结果表明 ,Ti Ta O薄膜涂层具有良好...利用磁控溅射技术合成了Ti Ta O薄膜涂层。采用体外血小板粘附试验、动态凝血时间测定以及动物体内试片埋植试验等评价方法 ,对涂层的抗凝血特性进行了研究 ;并采用Tauc法研究了涂层的禁带宽度。研究结果表明 ,Ti Ta O薄膜涂层具有良好的抗凝血特性以及禁带宽度为 3.2eV的半导体特性。此外 ,探讨了Ti Ta O涂层的抗凝血机理 ,并提出材料的半导体特性是影响Ti Ta O涂层抗凝血特性的主要原因之一。展开更多
文摘An antithrombus enzyme (ATE) was precipitated by (NH4)2SO4 or ethanol from supernatant of Bacillus subtilis culture broth then purified using ion exchange chromatography on CM-sepharose fast flow. The effects of ionic strength and pH value on protein adsorption, the gradient elution at different flow rates and step elution were examined respectively. The recovery yield of the optimised process was 74.5% with a purification factor 8.1. The ATE molecular weight was estimated as 30ku by SDS-PAGE. The experimental results showed that the enzyme was stable in the range of pH 7 to pH11, and temperature 25℃ to 37℃.
文摘利用磁控溅射技术合成了Ti Ta O薄膜涂层。采用体外血小板粘附试验、动态凝血时间测定以及动物体内试片埋植试验等评价方法 ,对涂层的抗凝血特性进行了研究 ;并采用Tauc法研究了涂层的禁带宽度。研究结果表明 ,Ti Ta O薄膜涂层具有良好的抗凝血特性以及禁带宽度为 3.2eV的半导体特性。此外 ,探讨了Ti Ta O涂层的抗凝血机理 ,并提出材料的半导体特性是影响Ti Ta O涂层抗凝血特性的主要原因之一。
