Background:Ischemic stroke(IS)is a global health issue and the current treatment options for IS are inadequate.Buyang Huanwu decoction(BHD)has demonstrated effectiveness in treating IS.However,the mechanisms by which ...Background:Ischemic stroke(IS)is a global health issue and the current treatment options for IS are inadequate.Buyang Huanwu decoction(BHD)has demonstrated effectiveness in treating IS.However,the mechanisms by which BHD treats IS remain unclear,and no studies have been conducted to analyze these mechanisms from the perspective of Cuproptosis.In order to investigate the potential of BHD to intervene in IS through Cuproptosis,this study employed a systematic pharmacological approach and molecular docking verification.Methods:To investigate the mechanism of BHD in treating IS through Cuproptosis,relevant information on the structure,targets,and major biological functions and pathways of compounds related to BHD was collected from databases such as PubChem,PharmMapper,UniProt,and GeneCards.The results were then visualized using Cytoscape3.6.1,Ledock,and pymol software.Results:BHD is composed of 7 Chinese medicines,which contain 82 compounds,including 10 core compounds.These compounds are associated with 241 genes,of which 97 are common to BHD,IS,and Cuproptosis.The 97 common genes,including 10 core genes,are involved in biological processes such as proteolysis,regulation of apoptosis and cholesterol storage,as well as cellular components and molecular functions.The common genes among BHD,IS,and Cuproptosis,including 10 core genes,participate mainly in Kyoto Encyclopedia of Genes and Genomes pathways such as pathways in cancer,PI3K-Akt signaling pathway,and Estrogen signaling pathway.According to molecular docking results,linolenic acid showed good docking scores with 9 out of 10 core genes,except for SRC.13-hydroxy-9,11-octadecadienoic acid also demonstrated good docking scores with EGFR,MAPK14,and F2.Similarly,senkyunone also had good docking scores with EGFR,MAPK14,and F2,all of which had docking energy greater than−5 kcal.Conclusion:In this study,the potential of BHD for treating IS through Cuproptosis and its underlying mechanisms were explored and partially validated through molecular docking.However,due to the limitations of the systems pharmacology research method,further validation through cell experiments,animal experiments,and clinical trials may be necessary to confirm these findings.展开更多
Objective:Systematically evaluate the effect of Buyang Huanwu decoction(BHD)combined with Western medicine(WM)in treating diabetic foot(DF).Methods:Randomized controlled trials that examined the effects of BHD on DF w...Objective:Systematically evaluate the effect of Buyang Huanwu decoction(BHD)combined with Western medicine(WM)in treating diabetic foot(DF).Methods:Randomized controlled trials that examined the effects of BHD on DF were collected from various databases,including CBM,PubMed,EMBASE,Web of Science,CNKI,Wanfang,and Weipu.Two researchers evaluated the quality of the literature and extracted relevant data,which was then analyzed using RevMan 5.3 for meta-analysis.The analysis was carried out in accordance with the 2020 PRISMA checklist.Results:The study analyzed 19 articles involving 1284 patients.According to the meta-analysis,BHD combined with WM improved clinical efficacy and reduced traditional Chinese medicine syndrome scores,glycosylated hemoglobin,and fibrinogen levels in DF patients.Although the BHD combined with WM showed a trend towards reducing wound area,the difference was not significant.Additionally,the incidence of adverse reactions did not show a significant decrease with BHD combined with WM.Conclusion:The findings of the study indicated that the use of BHD combined with WM yielded better therapeutic outcomes than WM alone,supporting the recommendation of using BHD with WM for DF treatment.This approach is suggested for further clinical application and promotion.展开更多
Objective:To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method.Methods:Twenty-four three-month-old kunming animals we...Objective:To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method.Methods:Twenty-four three-month-old kunming animals were selected as experimental samples and randomly divided into control group,model group and Buyang Huanwu Decoction according to their body weight.The memory level of experimental animals was detected by novel body recognition test,and the neuron structure of experimental animals was detected by HE staining.The plasma of each group of experimental animals was quantitatively analyzed by gas chromatography-mass spectrometry,and the important metabolites and main metabolic pathways in the process of pathological changes were traced by using plasma metabolism.Results:In HE staining,compared with blank group,hippocampal neurons in model group were disordered and morphologically abnormal.Compared with the model group,the hippocampal neurons in Buyang Huanwu Decoction group arranged regularly and had normal morphology.Compared with blank group,the index of new object recognition in model group was significantly decreased(P<0.05);Compared with model group,the new object recognition index of Buyang Huanwu Decoction group was significantly increased(P<0.05).Five different metabolites of AD were identified by GC-MS,which were L-pyroglutamate,lysine,pyrophosphoric acid,creatinine andα-lactose.Conclusion:Buyang Huanwu Decoction has a significant effect on D-gal model mice,and can effectively improve their learning and memory level.The mechanism may be related to glutathione metabolism and aminophyl biosynthesis.展开更多
Objective:To investigate the intervention effect of Buyang Huanwu Decoction on endothelial progenitor cell function(EPCs),and to explore the therapeutic mechanism of Buyang Huanwu Decoction.Methods:This research take ...Objective:To investigate the intervention effect of Buyang Huanwu Decoction on endothelial progenitor cell function(EPCs),and to explore the therapeutic mechanism of Buyang Huanwu Decoction.Methods:This research take 54 rats were divided into blank group,the control group,model group,Chinese medicine,western medicine group and combine traditional Chinese and western medicine group,tonifying Yang also five decoction and atorvastatin calcium for acute myocardial infarction(AMI)rats group intervention,by using the method of density gradient centrifugation separation training each rat endothelial progenitor cells,using tetramethyl azo salt trace enzyme reaction colorimetry,used in the determination of adhesion ability and improved Boyden chamber,the method of analysis and comparison between groups of endothelial progenitor cells proliferation,adhesion and migration.Results:In 7 days,14 days and 4 weeks,acute myocardial infarction model of rat peripheral blood EPCs count,proliferation,migration and adhesion ability were compared with control group were significantly decline,tonifying Yang also five decoction group,western medicine control group and combine traditional Chinese and western medicine group of peripheral blood EPCs count,proliferation,migration and adhesion ability compared with model group were significantly increased(P<0.05),and combine traditional Chinese and western medicine group is the traditional Chinese medicine and western medicine group on the improvement of the function of EPCs effect more significantly(P<0.05).Conclusion:Statins combined with Buyang Huanwu Decoction can better improve the endothelial function of AMI rats than traditional Chinese medicine or western medicine.Further clinical studies on statins may better improve the endothelial function of AMI patients,and provide a new research entry point for improving the long-term prognosis of AMI patients.展开更多
BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final e...BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final effect.In order to reveal the compatibility mechanism of BHD's prescription in treating and preventing ischemic cerebrovascular disease,we needed explore the effect and relation of ingredients in the prescription.OBJECTIVE:To observe the effect of Buyang Huanwu decoction(BHD)and Astragalus mongholicus on the activity of platelet activating factor receptor(PAFR)in the platelet of rabbits in vitro,and investigate the mechanism of Astragalus mongholicus.DESIGN:A decomposed recipes study.SETTING:Guangzhou University of Traditional Chinese Medicine.MATERIALS:Five New Zealand rabbits,weighing 2-3 kg,both sexes,were used.BHD was composed of Sheng Huang Qi 120 g,Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g,Hong Hua 3 g.The prescription for activating blood circulation consisted of Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g and Hong Hua 3 g.The prescription for invigorating qi consisted of 120 g Sheng Huang Qi.The prepared herbal pieces were purchased from the traditional Chinese medicine Dispensary of Foshan Second People's Hospital,and appraised by Professor Xu from Science of Chinese Materia Medica College,Guangzhou University of Traditional Chinese Medicine.3H-PAF was supplied by Amersham Co.,Ltd.(specific activity:6.475 TBq/mmol;batch number:200402);PAF standard by Biomol Co.,Ltd.(batch number:P1318V).METHODS:The experiments were carried out in the Laboratory of Nuclear Medicine,Guangzhou University of Traditional Chinese Medicine from September to December 2004.①Injections of BHD,prescriptions for activating blood circulation and invigorating qi were prepared by the decoction and alcohol sedimentation technique.Rabbit common carotid artery blood(40 mL)was drawn via intubation to prepare platelet suspension of (0.8-1.0) ×1010L-1.②Determination of 3H-PAF and washed PAFR binding:The general combination tube(T) contained washed platelet-rich plasma(WPRP)380μL +3H-PAF(0.35 nmol/L)10μL+distilled water 5μL:The nonspecific binding tube(P)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+cold PAF(1μmol/L)5μL:The sample tube(Y)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+experimental medicine(injection of BHD,prescriptions for activating blood circulation or invigorating qi)5μL.The test was conducted for three times for each sample in the same way as mentioned above.The samples were shaken on the oscillator for 30 s,then bathed at 25℃ for 40 minutes,and the reaction was terminated with cold Tris buffer containing 0.1% BSA.multichannel cell detachment separator was used for vacuum suction to filter the separated free 3H-PAF,and the filter paper was washed with cold Tris buffer for four times,then dried in the baking oven(80℃)for 1 hour,and placed in xylol liquid scintillator,and the radioactivity was determined automatically by the liquid scintillation detector.The mean of the three parallel tubes was calculated.The specific binging inhibition rate was calculated:SBIR=[(T-Y)/(T-P)]×100%].③Univariate analysis of variance was conducted.And for comparison of each paired groups,the q test was adopted.MAIN OUTCOME MEASURES:Effect of BHD whole prescription,prescriptions for activating blood circulation and invigorating qi on the specific binding inhibition rate of 3H-PAF and PAFR.RESULTS: BHD,prescriptions for activating blood circulation and invigorating qi were all able to inhibit the specific binding of 3H-PAF to PAFR,the specific blinding inhibition rates were(45.90±7.50)%.(97.90±1.84)% and(26.75±2.48)%,respectively,and there were significant differences between every two groups(P＜0.01).CONCLUSION:Single Astragalus mongholicus(120 g)can inhibit the specific blinding of PAFR in the platelet of the rabbit with 3H-PAF,but the combination of Astragalus mongholicus with the drugs for activating blood circulation in BHD can significantly decrease the inhibiting action of the latter on PAFR activity of the platelet,reflecting the combined mechanism of "removing blood stasis without injuring the vital qi"in BHD.展开更多
Background:Cerebrovascular accident has high morbidity,high disability,and high mortality,possibly causing several serious sequelae and bringing a heavy burden to the patient,the family,and the society.Buyang Huanwu d...Background:Cerebrovascular accident has high morbidity,high disability,and high mortality,possibly causing several serious sequelae and bringing a heavy burden to the patient,the family,and the society.Buyang Huanwu decoction,a classic ancient prescription of traditional Chinese medicine,and acupuncture are often used in combination to treat the sequelae of cerebrovascular accident.In the present study,we systematically evaluated the clinical efficacy and safety of acupuncture combined with Buyang Huanwu decoction in treating the sequelae of cerebrovascular accident.Methods:Relevant randomized controlled trials of acupuncture combined with Buyang Huanwu decoction in treating the sequelae of cerebrovascular accident were comprehensively retrieved from electronic databases from inception to May 8,2020,including PubMed,Embase,The Cochrane Library,China National Knowledge Infrastructure,WanFang Data,and China Science and Technology Journal Database.The main outcomes of clinical efficacy,degree of nerve function impairment,daily self-care capacity,Fugl-Meyer locomotor function score,life quality score,and occurrence of adverse reactions were subjected to meta-analysis.Results:The 28 randomized controlled trials included 2,341 patients with the sequelae of cerebrovascular accident.Systematic review and meta-analysis indicated that compared with acupuncture alone,its combined treatment with Buyang Huanwu decoction significantly increased clinical efficacy(RR=1.19,95%CI(1.14,1.23),P<0.001),improved nerve function impairment(WMD=−4.89,95%CI(−8.25,−1.53),P=0.004),promoted daily self-care capacity(WMD=15.87,95%CI(11.22,20.51),P<0.001),strengthened Fugl-Meyer locomotor function(WMD=20.89,95%CI(13.79,27.98),P<0.001),enhanced physical functioning(WMD=16.99,95%CI(6.01,27.96),P<0.001)and mental health(WMD=16.91,95%CI(9.14,24.69),P<0.001)in terms of life quality score(SF-36 scale)of patients with sequelae of cerebrovascular accident,with few adverse reactions.Conclusion:Compared with acupuncture alone,its combination with Buyang Huanwu decoction had better comprehensive effects on sequelae of cerebrovascular accident without obvious adverse reactions.展开更多
Objective:To evaluate the clinical efficacy of Buyang Huanwu decoction in treating animal model of cerebral ischemia.Methods:We searched PubMed,EMbase,Cochrane,CNKI,VIP,WanFang(1983 to 2021)for randomized control tria...Objective:To evaluate the clinical efficacy of Buyang Huanwu decoction in treating animal model of cerebral ischemia.Methods:We searched PubMed,EMbase,Cochrane,CNKI,VIP,WanFang(1983 to 2021)for randomized control trials about Buyang Huanwu decoction treating animal model of cerebral ischemia.Results:According to the inclusion and exclusion criteria and assessment the quality of included trials(RCTs),the extraction of effective data,7 randomized clinical trials and their efficiency were evaluated,statistical analysis was conducted by using RevMan 5.3 software.The outcome measures assessed were neurological score and/or infarction volume.Meta-analysis showed that the neurological behavior scores of Buyang Huanwu decoction on animal model of cerebral ischemia was lower than the control group(SMD=2.06,95%CI(1.75,2.37),P<0.00001);The cerebral infarct volume of Buyang Huanwu decoction on animal model of cerebral ischemia were smaller than the control group(SMD=4.08,95%CI(3.57,4.58),P<0.00001).Conclusion:It was effective by using Buyang Huanwu decoction to reduce neurological behavior scores and the volume of animal model of cerebral ischemia.展开更多
Objective: To explore the mechanisms of Buyang Huanwu Decoction(BYHWD) modulating the gut microbiome and trimethylamine oxide(TAMO) to exert cardioprotective effects. Methods: Ligation of the left anterior descending ...Objective: To explore the mechanisms of Buyang Huanwu Decoction(BYHWD) modulating the gut microbiome and trimethylamine oxide(TAMO) to exert cardioprotective effects. Methods: Ligation of the left anterior descending coronary artery was performed in rats to induce heart failure(HF). Except for the shamoperation group(n=10), 36 operation-induced models were randomized into 3 groups using a random number table(n=12 in each group): the model group, the BYHWD group(15.02 g/kg BYHWD), and the positive group(4.99 g/kg metoprolol succinate). After 4-week treatment(once daily by gavage), echocardiography was applied to evaluate the cardiac function and the Tei index(the ratio of ventricular isovolumic contraction time(IVCT)and isovolumic diastolic time(IVRT) to ejection time(ET)) was calculated;hematoxylin-eosin(HE) staining was observed to characterize the pathology of the myocardium and small intestinal villi. D-lactic acid was detected by an enzyme-linked immunosorbent assay(ELISA). Expressions of occludin, claudin-1, and zonula occludens(ZO-1) were detected by Western blot. 16S ribosomal ribonucleic acid(16S rRNA) sequencing was used to explore the changes in the intestinal flora. TMAO was detected via liquid chromatography-tandem mass spectrometry(LC-MS/MS). Results: In the echocardiography, the Tei index was considerably lower in the positive and BYHWD groups compared with the model group(P<0.05). Besides, BYHWD improved the pathology of myocardium and small intestine of HF rats and lowered the D-lactic acid content in the serum, when compared with the model group(P<0.05). BYHWD also improved the expression of occludin and claudin-1(P<0.05);in the gut microbiota analysis, BYHWD slowed down modifications in the structure distribution of gut microbiota and regulated the diversity of intestinal flora in HF rats. The content of TMAO in the serum was significantly lowered by BYWHT compared with the model group(P<0.05). Conclusion: BYHWD may delay progression of HF by enhancing the intestinal barrier structure, and regulating intestinal flora and TAMO.展开更多
OBJECTIVE:To evaluate the efficacy and safety of Buyang Huanwu decoction(BYHWD)in treating diabetic peripheral neuropathy(DPN).METHODS:Eight electronic databases,including China National Knowledge Infrastructure Datab...OBJECTIVE:To evaluate the efficacy and safety of Buyang Huanwu decoction(BYHWD)in treating diabetic peripheral neuropathy(DPN).METHODS:Eight electronic databases,including China National Knowledge Infrastructure Database,Wanfang Database,China Science and Technology Journal Database,Chinese Biomedical Literature Database,Cochrane Library,Embase,Web of Science,and PubM ed,were searched for randomized controlled trials(RCTs)of BYHWD to treat DPN.We identified all RCTs related to BYHWD and those on the treatment of DPN with the combination of mecobalamin.RevMan software was used for the statistical analysis.RESULTS:Twenty-one RCTs with a total of 1945 patients were included.The methodological quality of the literature included was low.Meta-analysis showed that the efficacy of the treatment group was significantly better than that of the control group in the treatment of DPN with BYHWD[risk ratio(RR)=0.33,95%CI(0.27,0.40),Z=11.25,P<0.00001].The median nerve of median motor nerve conduction velocity(MNCV)[mean difference(MD)=4.16,95%CI(1.35,6.98)]and median sensory NCV(SNCV)[(MD=3.28,95%CI(2.35,4.22)]were improved in the treatment group.The MNCV in the common peroneal nerve[(MD=1.63,95%CI(0.39,2.87)]and SNCV[(MD=4.56,95%CI(3.16,5.97)]were significantly higher than those in the control group(P<0.01).Plasma viscosity[(MD=-0.15,95%CI(-0.20,-0.09),Z=5.17,P<0.01)],whole blood high shear[(MD=-0.83,95%CI(-1.56,-0.11),Z=2.26,P=0.02)]and whole blood low shear[(MD=-1.61,95%CI(-2.28,-0.94),Z=4.68,P<0.01)]decreased significantly after treatment.There was no significant difference in fasting blood glucose[(MD=-0.42,95%CI(-0.89,0.05),Z=1.76,P=0.08)]between the treatment and control groups;postprandial blood glucose[(MD=-0.62,95%CI(-1.19,0.05),Z=2.12,P=0.03)]decreased significantly.No significant difference was found in the blood lipid levels between the treatment and control groups,including triglycerides[(MD=-0.21,95%CI(-0.52,0.10),Z=1.34,P=0.18)]and cholesterol[(MD=-0.13,95%CI(-0.27,0.00),Z=1.92,P=0.06)].Of the 21 RCTs,only five reported adverse reactions,and four studies reported the length of follow-up.No serious adverse events were reported.None of the studies reported the quality of life and economic conditions.CONCLUSIONS:Our study suggests that BYHWD has a significant therapeutic effect on DPN.High-quality,largescale RCTs are needed to provide more reliable evidence.展开更多
Objective To verify the effect of Buyang Huanwu Decoction(BHD)in ameliorating erectile dysfunction(ED)after radical prostatectomy(RP).Methods The composition of BHD was verified by ultra-performance liquid chromatogra...Objective To verify the effect of Buyang Huanwu Decoction(BHD)in ameliorating erectile dysfunction(ED)after radical prostatectomy(RP).Methods The composition of BHD was verified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS)analysis.Bilateral cavernous nerve crush injury(BCNI)in rats was used to mimic the neurovascular injury occurring after RP.By the envelope method,forty rats were randomly divided into 4 groups as follows:sham(cavernous nerves exposed only),model(BCNI),low-dosage BHD[LBHD,12.8 g/(kg·d)],and high-dosage BHD[HBHD,51.2 g/(kg·d)]groups,10 rats in each group,feeding for 3 weeks respectively.Erectile function was evaluated by measuring intracavernosal pressure(ICP).Changes in the histopathology of corpus cavernosum(CC)were examined by hematoxylin-eosin staining.Meanwhile,the fibrosis of CC was measured by Masson’s trichrome staining and Western blot was used to detect the expressions of collagen I,transforming growth factor beta 1(TGF-β1)andα-smooth muscle actin(α-SMA).Apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)and Western blot for determining the expressions of B-cell lymphoma 2(Bcl-2)and Bcl-2-associated X(Bax).The oxidative stress in the CC were assessed by the superoxide dismutase(SOD),malondialdehyde(MDA)and reactive oxygen species(ROS)levels.The proteins expression of c-Jun N-terminal kinase(JNK)and c-Jun were detected by Western blot.In addition,the expression ofα-SMA and p-c-Jun in the CC was observed by double immunofluorescence staining.Results The UPLC-QTOF-MS/MS analysis showed that BHD contained calycosin-7-O-β-D-glucoside,ononin,calycosin and formononetin.Compared with the model group,LBHD and HBHD treatment improved the ICP and the circumference,area,and weight of CC(P<0.05 or P<0.01).Furthermore,LBHD and HBHD treatments increased CC smooth muscle content and decreased apoptosis index(P<0.05 or P<0.01).LBHD and HBHD also elevated SOD and expression level ofα-SMA and Bcl-2,and reduced MDA and ROS levels,as well as expression of TGF-β1,collagen I,Bax,p-c-JNK,p-JNK in the CC compared with the model group(P<0.05 or P<0.01).The double immunofluorescence staining showed that the fluorescence degree of p-c-Jun in both LBHD and HBHD treatment groups was significantly reduced,whereas theα-SMA expression increased(P<0.05 or P<0.01).Conclusions BHD can improve ED of rats with BCNI,which is related to inhibiting fibrosis,apoptosis,and oxidative stress of CC.The ROS/JNK/c-Jun signaling pathway may play an important role in the process.展开更多
Objective:The objective of this study was to investigate the molecular mechanism of Buyang Huanwu decoction(BYHWD)in the treatment of diabetic foot(DF).Methods:The TCMSP,BATMAN,PubChem,PharmMapper,UniProt,GeneCards,We...Objective:The objective of this study was to investigate the molecular mechanism of Buyang Huanwu decoction(BYHWD)in the treatment of diabetic foot(DF).Methods:The TCMSP,BATMAN,PubChem,PharmMapper,UniProt,GeneCards,Webgestalt,and Kobas databases were used to obtain the structures,targets,main biological functions,and pathways of the active ingredients of BYHWD,and the results were visualized using Cytoscape3.6.1,Ledock,and PyMol software.Results:A total of 82 active components of BYHWD and 193 targets related to BYHWD were identified,and 5295 genes related to DF were identified using the GeneCards database,including 65 key targets of BYHWD in the treatment of DF.GO and KEGG enrichment analyses of the 65 targets for BYHWD treatment of DF showed that 47 GO items were involved in the treatment.It was mainly involved in biological processes,such as biological regulation,metabolism,and stress response.It is primarily involved in protein binding,ion binding,nucleotide binding,and other molecular functions.It is mainly involved in membrane encapsulation,membrane lumen closure,and other biological components and involved in the VEGF,TNF,RAS,RAP1,PI3K-AKT,MAPK,and IL-17 signaling pathways.Most targets were enriched in the PI3K-AKT and MAPK signaling pathways.Molecular docking results showed that the 59 key active components of BYHWD had strong binding activity with 64 key DF targets.Conclusion:The therapeutic effect of BYHWD on DF is based on the pharmacological effects of multiple targets and pathways.展开更多
AIM: To study the effect of Buyang Huanwu Decoction(BYHWD) on the antioxidant enzymes and drug-metabolizing enzymes in rat liver. METHOD: Following treatment of rats with BYHWD at 6.42, 12.83, or 25.66 g·kg–1 pe...AIM: To study the effect of Buyang Huanwu Decoction(BYHWD) on the antioxidant enzymes and drug-metabolizing enzymes in rat liver. METHOD: Following treatment of rats with BYHWD at 6.42, 12.83, or 25.66 g·kg–1 per day for 15 days, microsomes and cytosols isolated from the liver tissues were prepared by differential centrifugation according to standard procedures. The activities of the antioxidant enzymes and cytochrome b5, NADPH-cytochrome P450 reductase, CYP3 A, CYP2E1, UGT, and GST of the rat livers were determined by UV-Vis spectrophotometer. RESULTS: The activities of ALT, AST, antioxidant enzymes, and the Hepatosomatic Index in serum were not significantly affected. In cytosols, the activity of CAT was significantly increased at the dosage of 12.83 g·kg–1, and all the other antioxidant activities and MDA levels were not affected by this treatment. BYHWD had no effect on cytochrome b5, NADPH-cytochrome P450 reductase, CYP3 A, and UGT. At the highest dose(25.66 g·kg–1), the activity of CYP2E1 was significantly inhibited, and the activities of GST and the level of GSH were increased. CONCLUSION: BYHWD is safe for the liver, and has the functions of detoxification and antioxidant. Patients should be cautioned about the herb-drug interaction of BYHWD and CYP2E1 substrates.展开更多
Objective To conduct a comparative study on the brain pharmacokinetics of seven ingredients(i.e.senkyunolide A,ferulic acid,formononetin,calycosin,ononin,calycosin-O-β-D-glucopyranoside,and paeoniflorin),which were t...Objective To conduct a comparative study on the brain pharmacokinetics of seven ingredients(i.e.senkyunolide A,ferulic acid,formononetin,calycosin,ononin,calycosin-O-β-D-glucopyranoside,and paeoniflorin),which were the compounds of Buyang Huanwu Decoction(BHD),in normal and cerebral ischemia rats administrated intragastrically with BHD.Methods The samples of normal and permanent middle cerebral artery occlusion(pMCAO)rats were collected by using brain microdialysis technique.The concentrations of seven ingredients were determined by the HPLC-MS/MS method.After the BHD were administrated intragastrically to the rats for seven consecutive days,brain microdialysis probes were inserted into the hippocampus of rats,and then the brain microdialysates were collected at 20 min time intervals for 5 h.The separation of the seven ingredients and internal standard(IS)was carried out on an ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)chromatographic column,using a mobile phase consisting of acetonitrile(containing 0.1%formic acid)and water(containing 0.1%formic acid)for gradient elution within 13 min.The ionization was conducted using an ESI source in positive ion mode.Multiple reaction monitoring mode was used for quantification of ingredients in BHD.Results Linearity,accuracy,precision,matrix effect and stability of LC-MS/MS method were all satisfactory,successfully applied to compare the pharmacokinetics of the analytes between normal and model rats after intragastric administration of BHD.Compared with the normal group,the model group after the administration of the BHD showed that T1/2 of formononetin and ononin were longer,and except for calycosin-O-β-D-glucopyranoside(P<0.01),there was no significant difference between the normal group and the model group.The C_(max) of senkyunolide A and calycosin of model group were increased,while the T_(max) of senkyunolide A was decreased,and except for the T_(max) of PF,the differences between the two groups were statistically significant(P<0.01).Conclusion The LC-MS/MS method combined with microdialysis was successfully applied to the comparative study of brain pharmacokinetics of seven ingredients in BHD.After intragastric administration of BHD,there were differences in the pharmacokinetics of seven ingredients in the brain hippocampus between normal rats and model rats,probably related to the characteristics of the ingredients and the effects of cerebral ischemia on the absorption and distribution of the ingredients.展开更多
[Objectives]The paper was to investigate the clinical effect of Fuyang prescription in the treatment of yang deficiency syndrome in the convalescent stage of ischemic stroke(IS)and its mechanism of action.[Methods]A t...[Objectives]The paper was to investigate the clinical effect of Fuyang prescription in the treatment of yang deficiency syndrome in the convalescent stage of ischemic stroke(IS)and its mechanism of action.[Methods]A total of 50 patients with yang deficiency syndrome in the convalescent stage of IS were randomly divided into treatment group and control group,with 25 patients in each group.The patients in the treatment group were given basic treatment combined with Fuyang prescription,and those in the control group were given basic treatment combined with Buyang Huanwu decoction;the course of treatment was 28 d for both groups.The activities of daily living(ADL)score,National Institutes of Health Stroke Scale(NIHSS)score,quality of life score,TCM syndrome score,and safety in both groups were observed before treatment and at 14 and 28 d post treatment,and their clinical outcome was evaluated.[Results]There was a significant difference in overall response rate between the treatment group and the control group[92.00%(23/25)vs 76.00%(19/25),P<0.05].After treatment,both groups had significant changes in ADL score,NIHSS score,quality of life score,and TCM syndrome score,and there were significant differences in these indices between the two groups at the same time point after treatment(P<0.05 or P<0.01).There were no significant changes in routine blood,routine stool,routine urine,and liver/renal function in either group after treatment.[Conclusions]Fuyang prescription has a marked clinical effect in the treatment of yang deficiency syndrome in the convalescent stage of IS,with significantly better clinical effect than Buyang Huanwu decoction,and it provides clinical evidence for the traditional Chinese medicine theory of yang supporting in the treatment of IS in the convalescent stage.展开更多
基金This study was funded by the National Natural Science Foundation of China(81874416)Hunan Science and Technology Innovation Team Project(2020RC4050)+1 种基金Hunan Provincial Innovation Foundation for Postgraduate(CX20220788)Foshan Medical Research Fund(20210311).
文摘Background:Ischemic stroke(IS)is a global health issue and the current treatment options for IS are inadequate.Buyang Huanwu decoction(BHD)has demonstrated effectiveness in treating IS.However,the mechanisms by which BHD treats IS remain unclear,and no studies have been conducted to analyze these mechanisms from the perspective of Cuproptosis.In order to investigate the potential of BHD to intervene in IS through Cuproptosis,this study employed a systematic pharmacological approach and molecular docking verification.Methods:To investigate the mechanism of BHD in treating IS through Cuproptosis,relevant information on the structure,targets,and major biological functions and pathways of compounds related to BHD was collected from databases such as PubChem,PharmMapper,UniProt,and GeneCards.The results were then visualized using Cytoscape3.6.1,Ledock,and pymol software.Results:BHD is composed of 7 Chinese medicines,which contain 82 compounds,including 10 core compounds.These compounds are associated with 241 genes,of which 97 are common to BHD,IS,and Cuproptosis.The 97 common genes,including 10 core genes,are involved in biological processes such as proteolysis,regulation of apoptosis and cholesterol storage,as well as cellular components and molecular functions.The common genes among BHD,IS,and Cuproptosis,including 10 core genes,participate mainly in Kyoto Encyclopedia of Genes and Genomes pathways such as pathways in cancer,PI3K-Akt signaling pathway,and Estrogen signaling pathway.According to molecular docking results,linolenic acid showed good docking scores with 9 out of 10 core genes,except for SRC.13-hydroxy-9,11-octadecadienoic acid also demonstrated good docking scores with EGFR,MAPK14,and F2.Similarly,senkyunone also had good docking scores with EGFR,MAPK14,and F2,all of which had docking energy greater than−5 kcal.Conclusion:In this study,the potential of BHD for treating IS through Cuproptosis and its underlying mechanisms were explored and partially validated through molecular docking.However,due to the limitations of the systems pharmacology research method,further validation through cell experiments,animal experiments,and clinical trials may be necessary to confirm these findings.
基金This study was funded by the National Natural Science Foundation of China(81874416)Hunan Science and Technology Innovation Team Project(2020RC4050)Hunan Provincial Innovation Foundation for Postgraduate(CX20220788).
文摘Objective:Systematically evaluate the effect of Buyang Huanwu decoction(BHD)combined with Western medicine(WM)in treating diabetic foot(DF).Methods:Randomized controlled trials that examined the effects of BHD on DF were collected from various databases,including CBM,PubMed,EMBASE,Web of Science,CNKI,Wanfang,and Weipu.Two researchers evaluated the quality of the literature and extracted relevant data,which was then analyzed using RevMan 5.3 for meta-analysis.The analysis was carried out in accordance with the 2020 PRISMA checklist.Results:The study analyzed 19 articles involving 1284 patients.According to the meta-analysis,BHD combined with WM improved clinical efficacy and reduced traditional Chinese medicine syndrome scores,glycosylated hemoglobin,and fibrinogen levels in DF patients.Although the BHD combined with WM showed a trend towards reducing wound area,the difference was not significant.Additionally,the incidence of adverse reactions did not show a significant decrease with BHD combined with WM.Conclusion:The findings of the study indicated that the use of BHD combined with WM yielded better therapeutic outcomes than WM alone,supporting the recommendation of using BHD with WM for DF treatment.This approach is suggested for further clinical application and promotion.
基金National Natural Science Foundation of China(No.82174007)Talent Training Project Supported by the Central Government for the Reform and Development of Local Universities+2 种基金Research Project of Heilongjiang Provincial Health Commission(No.2020-303)National Natural Science Foundation of China(No.82003975)Fund Project of Heilongjiang University of Traditional Chinese Medicine(No.2018RCD19,2018pt04)。
文摘Objective:To study the protective effect of Buyang Huanwu Decoction on the learning and memory ability of D-gal induced aging mice using GC-MS metabolomics method.Methods:Twenty-four three-month-old kunming animals were selected as experimental samples and randomly divided into control group,model group and Buyang Huanwu Decoction according to their body weight.The memory level of experimental animals was detected by novel body recognition test,and the neuron structure of experimental animals was detected by HE staining.The plasma of each group of experimental animals was quantitatively analyzed by gas chromatography-mass spectrometry,and the important metabolites and main metabolic pathways in the process of pathological changes were traced by using plasma metabolism.Results:In HE staining,compared with blank group,hippocampal neurons in model group were disordered and morphologically abnormal.Compared with the model group,the hippocampal neurons in Buyang Huanwu Decoction group arranged regularly and had normal morphology.Compared with blank group,the index of new object recognition in model group was significantly decreased(P<0.05);Compared with model group,the new object recognition index of Buyang Huanwu Decoction group was significantly increased(P<0.05).Five different metabolites of AD were identified by GC-MS,which were L-pyroglutamate,lysine,pyrophosphoric acid,creatinine andα-lactose.Conclusion:Buyang Huanwu Decoction has a significant effect on D-gal model mice,and can effectively improve their learning and memory level.The mechanism may be related to glutathione metabolism and aminophyl biosynthesis.
基金Regional fund project of the national natural science foundation of China(No.81660778)。
文摘Objective:To investigate the intervention effect of Buyang Huanwu Decoction on endothelial progenitor cell function(EPCs),and to explore the therapeutic mechanism of Buyang Huanwu Decoction.Methods:This research take 54 rats were divided into blank group,the control group,model group,Chinese medicine,western medicine group and combine traditional Chinese and western medicine group,tonifying Yang also five decoction and atorvastatin calcium for acute myocardial infarction(AMI)rats group intervention,by using the method of density gradient centrifugation separation training each rat endothelial progenitor cells,using tetramethyl azo salt trace enzyme reaction colorimetry,used in the determination of adhesion ability and improved Boyden chamber,the method of analysis and comparison between groups of endothelial progenitor cells proliferation,adhesion and migration.Results:In 7 days,14 days and 4 weeks,acute myocardial infarction model of rat peripheral blood EPCs count,proliferation,migration and adhesion ability were compared with control group were significantly decline,tonifying Yang also five decoction group,western medicine control group and combine traditional Chinese and western medicine group of peripheral blood EPCs count,proliferation,migration and adhesion ability compared with model group were significantly increased(P<0.05),and combine traditional Chinese and western medicine group is the traditional Chinese medicine and western medicine group on the improvement of the function of EPCs effect more significantly(P<0.05).Conclusion:Statins combined with Buyang Huanwu Decoction can better improve the endothelial function of AMI rats than traditional Chinese medicine or western medicine.Further clinical studies on statins may better improve the endothelial function of AMI patients,and provide a new research entry point for improving the long-term prognosis of AMI patients.
基金grants from Scientific Planning Program of Guangdong Province, No. 2004B36001009Scientific Research Funds of Guangdong Bureau of Traditional Chinese Medicine, No. 30002+1 种基金 Scientific Development Special Planning Funds of Foshan City, No. 200124Medical Scientific Research Program of Foshan City, No. 2000096
文摘BACKGROUND:The pharmacological action of traditional Chinese medicine compound is the comprehensive effect of the various ingredients,and the interactions of various ingredients are closely correlated with the final effect.In order to reveal the compatibility mechanism of BHD's prescription in treating and preventing ischemic cerebrovascular disease,we needed explore the effect and relation of ingredients in the prescription.OBJECTIVE:To observe the effect of Buyang Huanwu decoction(BHD)and Astragalus mongholicus on the activity of platelet activating factor receptor(PAFR)in the platelet of rabbits in vitro,and investigate the mechanism of Astragalus mongholicus.DESIGN:A decomposed recipes study.SETTING:Guangzhou University of Traditional Chinese Medicine.MATERIALS:Five New Zealand rabbits,weighing 2-3 kg,both sexes,were used.BHD was composed of Sheng Huang Qi 120 g,Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g,Hong Hua 3 g.The prescription for activating blood circulation consisted of Dang Gui Wei 6 g,Chi Shao 4.5 g,Chuan Xiong 3 g,Di Long 3 g,Tao Ren 3 g and Hong Hua 3 g.The prescription for invigorating qi consisted of 120 g Sheng Huang Qi.The prepared herbal pieces were purchased from the traditional Chinese medicine Dispensary of Foshan Second People's Hospital,and appraised by Professor Xu from Science of Chinese Materia Medica College,Guangzhou University of Traditional Chinese Medicine.3H-PAF was supplied by Amersham Co.,Ltd.(specific activity:6.475 TBq/mmol;batch number:200402);PAF standard by Biomol Co.,Ltd.(batch number:P1318V).METHODS:The experiments were carried out in the Laboratory of Nuclear Medicine,Guangzhou University of Traditional Chinese Medicine from September to December 2004.①Injections of BHD,prescriptions for activating blood circulation and invigorating qi were prepared by the decoction and alcohol sedimentation technique.Rabbit common carotid artery blood(40 mL)was drawn via intubation to prepare platelet suspension of (0.8-1.0) ×1010L-1.②Determination of 3H-PAF and washed PAFR binding:The general combination tube(T) contained washed platelet-rich plasma(WPRP)380μL +3H-PAF(0.35 nmol/L)10μL+distilled water 5μL:The nonspecific binding tube(P)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+cold PAF(1μmol/L)5μL:The sample tube(Y)contained WPRP 380μL+3H-PAF(0.35 nmol/L)10μL+experimental medicine(injection of BHD,prescriptions for activating blood circulation or invigorating qi)5μL.The test was conducted for three times for each sample in the same way as mentioned above.The samples were shaken on the oscillator for 30 s,then bathed at 25℃ for 40 minutes,and the reaction was terminated with cold Tris buffer containing 0.1% BSA.multichannel cell detachment separator was used for vacuum suction to filter the separated free 3H-PAF,and the filter paper was washed with cold Tris buffer for four times,then dried in the baking oven(80℃)for 1 hour,and placed in xylol liquid scintillator,and the radioactivity was determined automatically by the liquid scintillation detector.The mean of the three parallel tubes was calculated.The specific binging inhibition rate was calculated:SBIR=[(T-Y)/(T-P)]×100%].③Univariate analysis of variance was conducted.And for comparison of each paired groups,the q test was adopted.MAIN OUTCOME MEASURES:Effect of BHD whole prescription,prescriptions for activating blood circulation and invigorating qi on the specific binding inhibition rate of 3H-PAF and PAFR.RESULTS: BHD,prescriptions for activating blood circulation and invigorating qi were all able to inhibit the specific binding of 3H-PAF to PAFR,the specific blinding inhibition rates were(45.90±7.50)%.(97.90±1.84)% and(26.75±2.48)%,respectively,and there were significant differences between every two groups(P＜0.01).CONCLUSION:Single Astragalus mongholicus(120 g)can inhibit the specific blinding of PAFR in the platelet of the rabbit with 3H-PAF,but the combination of Astragalus mongholicus with the drugs for activating blood circulation in BHD can significantly decrease the inhibiting action of the latter on PAFR activity of the platelet,reflecting the combined mechanism of "removing blood stasis without injuring the vital qi"in BHD.
基金This study was supported by Fundamental Research Funds for the Central University(No.GK202003050,GK202003048)the Innovation Training Program for University Student(No.cx2019013,s201910718084).
文摘Background:Cerebrovascular accident has high morbidity,high disability,and high mortality,possibly causing several serious sequelae and bringing a heavy burden to the patient,the family,and the society.Buyang Huanwu decoction,a classic ancient prescription of traditional Chinese medicine,and acupuncture are often used in combination to treat the sequelae of cerebrovascular accident.In the present study,we systematically evaluated the clinical efficacy and safety of acupuncture combined with Buyang Huanwu decoction in treating the sequelae of cerebrovascular accident.Methods:Relevant randomized controlled trials of acupuncture combined with Buyang Huanwu decoction in treating the sequelae of cerebrovascular accident were comprehensively retrieved from electronic databases from inception to May 8,2020,including PubMed,Embase,The Cochrane Library,China National Knowledge Infrastructure,WanFang Data,and China Science and Technology Journal Database.The main outcomes of clinical efficacy,degree of nerve function impairment,daily self-care capacity,Fugl-Meyer locomotor function score,life quality score,and occurrence of adverse reactions were subjected to meta-analysis.Results:The 28 randomized controlled trials included 2,341 patients with the sequelae of cerebrovascular accident.Systematic review and meta-analysis indicated that compared with acupuncture alone,its combined treatment with Buyang Huanwu decoction significantly increased clinical efficacy(RR=1.19,95%CI(1.14,1.23),P<0.001),improved nerve function impairment(WMD=−4.89,95%CI(−8.25,−1.53),P=0.004),promoted daily self-care capacity(WMD=15.87,95%CI(11.22,20.51),P<0.001),strengthened Fugl-Meyer locomotor function(WMD=20.89,95%CI(13.79,27.98),P<0.001),enhanced physical functioning(WMD=16.99,95%CI(6.01,27.96),P<0.001)and mental health(WMD=16.91,95%CI(9.14,24.69),P<0.001)in terms of life quality score(SF-36 scale)of patients with sequelae of cerebrovascular accident,with few adverse reactions.Conclusion:Compared with acupuncture alone,its combination with Buyang Huanwu decoction had better comprehensive effects on sequelae of cerebrovascular accident without obvious adverse reactions.
文摘Objective:To evaluate the clinical efficacy of Buyang Huanwu decoction in treating animal model of cerebral ischemia.Methods:We searched PubMed,EMbase,Cochrane,CNKI,VIP,WanFang(1983 to 2021)for randomized control trials about Buyang Huanwu decoction treating animal model of cerebral ischemia.Results:According to the inclusion and exclusion criteria and assessment the quality of included trials(RCTs),the extraction of effective data,7 randomized clinical trials and their efficiency were evaluated,statistical analysis was conducted by using RevMan 5.3 software.The outcome measures assessed were neurological score and/or infarction volume.Meta-analysis showed that the neurological behavior scores of Buyang Huanwu decoction on animal model of cerebral ischemia was lower than the control group(SMD=2.06,95%CI(1.75,2.37),P<0.00001);The cerebral infarct volume of Buyang Huanwu decoction on animal model of cerebral ischemia were smaller than the control group(SMD=4.08,95%CI(3.57,4.58),P<0.00001).Conclusion:It was effective by using Buyang Huanwu decoction to reduce neurological behavior scores and the volume of animal model of cerebral ischemia.
基金Supported by China Key R&D Program of the Ministry of Science and Technology(No.2017YFC1700206)。
文摘Objective: To explore the mechanisms of Buyang Huanwu Decoction(BYHWD) modulating the gut microbiome and trimethylamine oxide(TAMO) to exert cardioprotective effects. Methods: Ligation of the left anterior descending coronary artery was performed in rats to induce heart failure(HF). Except for the shamoperation group(n=10), 36 operation-induced models were randomized into 3 groups using a random number table(n=12 in each group): the model group, the BYHWD group(15.02 g/kg BYHWD), and the positive group(4.99 g/kg metoprolol succinate). After 4-week treatment(once daily by gavage), echocardiography was applied to evaluate the cardiac function and the Tei index(the ratio of ventricular isovolumic contraction time(IVCT)and isovolumic diastolic time(IVRT) to ejection time(ET)) was calculated;hematoxylin-eosin(HE) staining was observed to characterize the pathology of the myocardium and small intestinal villi. D-lactic acid was detected by an enzyme-linked immunosorbent assay(ELISA). Expressions of occludin, claudin-1, and zonula occludens(ZO-1) were detected by Western blot. 16S ribosomal ribonucleic acid(16S rRNA) sequencing was used to explore the changes in the intestinal flora. TMAO was detected via liquid chromatography-tandem mass spectrometry(LC-MS/MS). Results: In the echocardiography, the Tei index was considerably lower in the positive and BYHWD groups compared with the model group(P<0.05). Besides, BYHWD improved the pathology of myocardium and small intestine of HF rats and lowered the D-lactic acid content in the serum, when compared with the model group(P<0.05). BYHWD also improved the expression of occludin and claudin-1(P<0.05);in the gut microbiota analysis, BYHWD slowed down modifications in the structure distribution of gut microbiota and regulated the diversity of intestinal flora in HF rats. The content of TMAO in the serum was significantly lowered by BYWHT compared with the model group(P<0.05). Conclusion: BYHWD may delay progression of HF by enhancing the intestinal barrier structure, and regulating intestinal flora and TAMO.
基金Natural Science Foundation-funded Project:Optimization of Distribution of Anti-Diabetes Traditional Chinese Medicine based on Glucose Metabolism Signal Pathway and Uniform Design(No.7182143)。
文摘OBJECTIVE:To evaluate the efficacy and safety of Buyang Huanwu decoction(BYHWD)in treating diabetic peripheral neuropathy(DPN).METHODS:Eight electronic databases,including China National Knowledge Infrastructure Database,Wanfang Database,China Science and Technology Journal Database,Chinese Biomedical Literature Database,Cochrane Library,Embase,Web of Science,and PubM ed,were searched for randomized controlled trials(RCTs)of BYHWD to treat DPN.We identified all RCTs related to BYHWD and those on the treatment of DPN with the combination of mecobalamin.RevMan software was used for the statistical analysis.RESULTS:Twenty-one RCTs with a total of 1945 patients were included.The methodological quality of the literature included was low.Meta-analysis showed that the efficacy of the treatment group was significantly better than that of the control group in the treatment of DPN with BYHWD[risk ratio(RR)=0.33,95%CI(0.27,0.40),Z=11.25,P<0.00001].The median nerve of median motor nerve conduction velocity(MNCV)[mean difference(MD)=4.16,95%CI(1.35,6.98)]and median sensory NCV(SNCV)[(MD=3.28,95%CI(2.35,4.22)]were improved in the treatment group.The MNCV in the common peroneal nerve[(MD=1.63,95%CI(0.39,2.87)]and SNCV[(MD=4.56,95%CI(3.16,5.97)]were significantly higher than those in the control group(P<0.01).Plasma viscosity[(MD=-0.15,95%CI(-0.20,-0.09),Z=5.17,P<0.01)],whole blood high shear[(MD=-0.83,95%CI(-1.56,-0.11),Z=2.26,P=0.02)]and whole blood low shear[(MD=-1.61,95%CI(-2.28,-0.94),Z=4.68,P<0.01)]decreased significantly after treatment.There was no significant difference in fasting blood glucose[(MD=-0.42,95%CI(-0.89,0.05),Z=1.76,P=0.08)]between the treatment and control groups;postprandial blood glucose[(MD=-0.62,95%CI(-1.19,0.05),Z=2.12,P=0.03)]decreased significantly.No significant difference was found in the blood lipid levels between the treatment and control groups,including triglycerides[(MD=-0.21,95%CI(-0.52,0.10),Z=1.34,P=0.18)]and cholesterol[(MD=-0.13,95%CI(-0.27,0.00),Z=1.92,P=0.06)].Of the 21 RCTs,only five reported adverse reactions,and four studies reported the length of follow-up.No serious adverse events were reported.None of the studies reported the quality of life and economic conditions.CONCLUSIONS:Our study suggests that BYHWD has a significant therapeutic effect on DPN.High-quality,largescale RCTs are needed to provide more reliable evidence.
基金Supported by National Natural Science Foundation of China(No.82004360,81804092)Zhejiang Provincial Natural Science Foundation(No.LY19H270011,LY18H270002,LGF20H270002)+1 种基金Doctoral Research Foundation of Affiliated Hospital of Nantong University(No.Tdb2006)Wenling City Science and Technology Plan Project(No.2021S00219)。
文摘Objective To verify the effect of Buyang Huanwu Decoction(BHD)in ameliorating erectile dysfunction(ED)after radical prostatectomy(RP).Methods The composition of BHD was verified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS/MS)analysis.Bilateral cavernous nerve crush injury(BCNI)in rats was used to mimic the neurovascular injury occurring after RP.By the envelope method,forty rats were randomly divided into 4 groups as follows:sham(cavernous nerves exposed only),model(BCNI),low-dosage BHD[LBHD,12.8 g/(kg·d)],and high-dosage BHD[HBHD,51.2 g/(kg·d)]groups,10 rats in each group,feeding for 3 weeks respectively.Erectile function was evaluated by measuring intracavernosal pressure(ICP).Changes in the histopathology of corpus cavernosum(CC)were examined by hematoxylin-eosin staining.Meanwhile,the fibrosis of CC was measured by Masson’s trichrome staining and Western blot was used to detect the expressions of collagen I,transforming growth factor beta 1(TGF-β1)andα-smooth muscle actin(α-SMA).Apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL)and Western blot for determining the expressions of B-cell lymphoma 2(Bcl-2)and Bcl-2-associated X(Bax).The oxidative stress in the CC were assessed by the superoxide dismutase(SOD),malondialdehyde(MDA)and reactive oxygen species(ROS)levels.The proteins expression of c-Jun N-terminal kinase(JNK)and c-Jun were detected by Western blot.In addition,the expression ofα-SMA and p-c-Jun in the CC was observed by double immunofluorescence staining.Results The UPLC-QTOF-MS/MS analysis showed that BHD contained calycosin-7-O-β-D-glucoside,ononin,calycosin and formononetin.Compared with the model group,LBHD and HBHD treatment improved the ICP and the circumference,area,and weight of CC(P<0.05 or P<0.01).Furthermore,LBHD and HBHD treatments increased CC smooth muscle content and decreased apoptosis index(P<0.05 or P<0.01).LBHD and HBHD also elevated SOD and expression level ofα-SMA and Bcl-2,and reduced MDA and ROS levels,as well as expression of TGF-β1,collagen I,Bax,p-c-JNK,p-JNK in the CC compared with the model group(P<0.05 or P<0.01).The double immunofluorescence staining showed that the fluorescence degree of p-c-Jun in both LBHD and HBHD treatment groups was significantly reduced,whereas theα-SMA expression increased(P<0.05 or P<0.01).Conclusions BHD can improve ED of rats with BCNI,which is related to inhibiting fibrosis,apoptosis,and oxidative stress of CC.The ROS/JNK/c-Jun signaling pathway may play an important role in the process.
基金funded by the National Natural Science Foundation of China(grant number 81874416).
文摘Objective:The objective of this study was to investigate the molecular mechanism of Buyang Huanwu decoction(BYHWD)in the treatment of diabetic foot(DF).Methods:The TCMSP,BATMAN,PubChem,PharmMapper,UniProt,GeneCards,Webgestalt,and Kobas databases were used to obtain the structures,targets,main biological functions,and pathways of the active ingredients of BYHWD,and the results were visualized using Cytoscape3.6.1,Ledock,and PyMol software.Results:A total of 82 active components of BYHWD and 193 targets related to BYHWD were identified,and 5295 genes related to DF were identified using the GeneCards database,including 65 key targets of BYHWD in the treatment of DF.GO and KEGG enrichment analyses of the 65 targets for BYHWD treatment of DF showed that 47 GO items were involved in the treatment.It was mainly involved in biological processes,such as biological regulation,metabolism,and stress response.It is primarily involved in protein binding,ion binding,nucleotide binding,and other molecular functions.It is mainly involved in membrane encapsulation,membrane lumen closure,and other biological components and involved in the VEGF,TNF,RAS,RAP1,PI3K-AKT,MAPK,and IL-17 signaling pathways.Most targets were enriched in the PI3K-AKT and MAPK signaling pathways.Molecular docking results showed that the 59 key active components of BYHWD had strong binding activity with 64 key DF targets.Conclusion:The therapeutic effect of BYHWD on DF is based on the pharmacological effects of multiple targets and pathways.
基金supported by the National Natural Science Foundation of China(No.81060353)Program for New Century Excellent Talent in University(NCET)of China from Ministry of Education of China(Doc.2010-14,NCET-10-0093)Natural Science Foundation of Guangxi province of China(No.2011GXNSFF018006)
文摘AIM: To study the effect of Buyang Huanwu Decoction(BYHWD) on the antioxidant enzymes and drug-metabolizing enzymes in rat liver. METHOD: Following treatment of rats with BYHWD at 6.42, 12.83, or 25.66 g·kg–1 per day for 15 days, microsomes and cytosols isolated from the liver tissues were prepared by differential centrifugation according to standard procedures. The activities of the antioxidant enzymes and cytochrome b5, NADPH-cytochrome P450 reductase, CYP3 A, CYP2E1, UGT, and GST of the rat livers were determined by UV-Vis spectrophotometer. RESULTS: The activities of ALT, AST, antioxidant enzymes, and the Hepatosomatic Index in serum were not significantly affected. In cytosols, the activity of CAT was significantly increased at the dosage of 12.83 g·kg–1, and all the other antioxidant activities and MDA levels were not affected by this treatment. BYHWD had no effect on cytochrome b5, NADPH-cytochrome P450 reductase, CYP3 A, and UGT. At the highest dose(25.66 g·kg–1), the activity of CYP2E1 was significantly inhibited, and the activities of GST and the level of GSH were increased. CONCLUSION: BYHWD is safe for the liver, and has the functions of detoxification and antioxidant. Patients should be cautioned about the herb-drug interaction of BYHWD and CYP2E1 substrates.
基金supported by the National Natural Science Foundation of China(No.81873227)。
文摘Objective To conduct a comparative study on the brain pharmacokinetics of seven ingredients(i.e.senkyunolide A,ferulic acid,formononetin,calycosin,ononin,calycosin-O-β-D-glucopyranoside,and paeoniflorin),which were the compounds of Buyang Huanwu Decoction(BHD),in normal and cerebral ischemia rats administrated intragastrically with BHD.Methods The samples of normal and permanent middle cerebral artery occlusion(pMCAO)rats were collected by using brain microdialysis technique.The concentrations of seven ingredients were determined by the HPLC-MS/MS method.After the BHD were administrated intragastrically to the rats for seven consecutive days,brain microdialysis probes were inserted into the hippocampus of rats,and then the brain microdialysates were collected at 20 min time intervals for 5 h.The separation of the seven ingredients and internal standard(IS)was carried out on an ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)chromatographic column,using a mobile phase consisting of acetonitrile(containing 0.1%formic acid)and water(containing 0.1%formic acid)for gradient elution within 13 min.The ionization was conducted using an ESI source in positive ion mode.Multiple reaction monitoring mode was used for quantification of ingredients in BHD.Results Linearity,accuracy,precision,matrix effect and stability of LC-MS/MS method were all satisfactory,successfully applied to compare the pharmacokinetics of the analytes between normal and model rats after intragastric administration of BHD.Compared with the normal group,the model group after the administration of the BHD showed that T1/2 of formononetin and ononin were longer,and except for calycosin-O-β-D-glucopyranoside(P<0.01),there was no significant difference between the normal group and the model group.The C_(max) of senkyunolide A and calycosin of model group were increased,while the T_(max) of senkyunolide A was decreased,and except for the T_(max) of PF,the differences between the two groups were statistically significant(P<0.01).Conclusion The LC-MS/MS method combined with microdialysis was successfully applied to the comparative study of brain pharmacokinetics of seven ingredients in BHD.After intragastric administration of BHD,there were differences in the pharmacokinetics of seven ingredients in the brain hippocampus between normal rats and model rats,probably related to the characteristics of the ingredients and the effects of cerebral ischemia on the absorption and distribution of the ingredients.
基金Supported by National Natural Science Foundation of China(81874453)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2020JJB140540)+5 种基金Innovation Team of Clinical Medicine Research and Application of Zhuang Medical Toxicities(04B22058A3)Guangxi Clinical Research Center for Encephalopathy of Traditional Chinese Medicine(AD20238028)The Second Batch of"Qihuang Project"High-level Talent Team Cultivation Project of Guangxi University of Traditional Chinese Medicine(2021008)Chinese Medicine Self-financing Project of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GXZYZ20210350)Graduate Education Innovation Program of Guangxi University of Traditional Chinese Medicine(YCBZ2021076)Hospital-level Project of Guangxi International Zhuang Medical Hospital(GZ2021007)。
文摘[Objectives]The paper was to investigate the clinical effect of Fuyang prescription in the treatment of yang deficiency syndrome in the convalescent stage of ischemic stroke(IS)and its mechanism of action.[Methods]A total of 50 patients with yang deficiency syndrome in the convalescent stage of IS were randomly divided into treatment group and control group,with 25 patients in each group.The patients in the treatment group were given basic treatment combined with Fuyang prescription,and those in the control group were given basic treatment combined with Buyang Huanwu decoction;the course of treatment was 28 d for both groups.The activities of daily living(ADL)score,National Institutes of Health Stroke Scale(NIHSS)score,quality of life score,TCM syndrome score,and safety in both groups were observed before treatment and at 14 and 28 d post treatment,and their clinical outcome was evaluated.[Results]There was a significant difference in overall response rate between the treatment group and the control group[92.00%(23/25)vs 76.00%(19/25),P<0.05].After treatment,both groups had significant changes in ADL score,NIHSS score,quality of life score,and TCM syndrome score,and there were significant differences in these indices between the two groups at the same time point after treatment(P<0.05 or P<0.01).There were no significant changes in routine blood,routine stool,routine urine,and liver/renal function in either group after treatment.[Conclusions]Fuyang prescription has a marked clinical effect in the treatment of yang deficiency syndrome in the convalescent stage of IS,with significantly better clinical effect than Buyang Huanwu decoction,and it provides clinical evidence for the traditional Chinese medicine theory of yang supporting in the treatment of IS in the convalescent stage.
