GABAergic input to Gonadotropin-releasing hormone (GnRH) neurons is necessary to initiate the onset of puberty and its action mainly depends on GABAA receptor of which the subunit composition, properties and consequen...GABAergic input to Gonadotropin-releasing hormone (GnRH) neurons is necessary to initiate the onset of puberty and its action mainly depends on GABAA receptor of which the subunit composition, properties and consequently function varies during this period. Nourishing “Yin”-Removing “Fire” Chinese herb mixture, a Chinese herb-based formulation, has been proved that it may retard the initiation of pubertal development in female precocious puberty rats. Our objective is to investigate the effects of Nourishing “Yin”-Removing “Fire” Chinese herb mixture on the expression of GABAA receptor α subunits in hypothalamus. Female Sprague-Dawley rats were divided into normal (N), precocious puberty model (M) induced by danazol, model exposed to saline (MS) and model exposed to Chinese herb mixture (CHM) groups. All rats were administered by the Chinese herb mixture from P15 on. Coefficients of reproductive organs and serum gonadotropins and estradiol levels in M were significantly enhanced while they were significantly decreased in CHM. The hypothalamic GnRH mRNA was also significantly increased in M and in CHM, as well as ERα mRNA. At the mean time, the hypothalamic GABAA receptor α1 and α3 subunits mRNA were more significantly decreased in M than those of N, while they were more significantly enhanced in CHM than those in M (p 0.01), the protein expression of which in hypothalamus had the same trend as the mRNA expression. The evidence suggests that Nourishing “Yin”-Removing “Fire” Chinese herb mixture could significantly retard the sexual development of the precocious rats, and up-regulate the expressions of hypothalamic GABAA receptor α1 and α3 subunits. Our result indicated that GABAA receptor α1 and α3 subunits might involve in the effective treatment of herb mixture on idiopathic precocious puberty.展开更多
Combined acupuncture and Chinese herb decoction were applied for 5 cases with graphospasm.Acupuncture included scalp acupuncture and body acupuncture.Using transcranial repeated needling,Baihui(百会GV20),Chorea-trembl...Combined acupuncture and Chinese herb decoction were applied for 5 cases with graphospasm.Acupuncture included scalp acupuncture and body acupuncture.Using transcranial repeated needling,Baihui(百会GV20),Chorea-trembling controlled area,Emotional area,as well as Dazhui(大椎GV14),Fengchi(风池GB20),and Yingxiang(迎香EX-HN8)etc.were stimulated by proposed by Sun Shentian.Meanwhile,the patients took orally Chinese herb decoction,on the basis of the Siwu decoction(四物汤four substances decoction)addition and subtraction,relieve symptoms under syndrome differentiation.The treatment was carried out for 4 courses in total.One course consisted of 5 days,with an interval of 2 days between two courses.After treatment of 5 patients,2 patients were cured,2 patients achieved markedly effective response,and 1 patient achieved effective response.展开更多
文摘GABAergic input to Gonadotropin-releasing hormone (GnRH) neurons is necessary to initiate the onset of puberty and its action mainly depends on GABAA receptor of which the subunit composition, properties and consequently function varies during this period. Nourishing “Yin”-Removing “Fire” Chinese herb mixture, a Chinese herb-based formulation, has been proved that it may retard the initiation of pubertal development in female precocious puberty rats. Our objective is to investigate the effects of Nourishing “Yin”-Removing “Fire” Chinese herb mixture on the expression of GABAA receptor α subunits in hypothalamus. Female Sprague-Dawley rats were divided into normal (N), precocious puberty model (M) induced by danazol, model exposed to saline (MS) and model exposed to Chinese herb mixture (CHM) groups. All rats were administered by the Chinese herb mixture from P15 on. Coefficients of reproductive organs and serum gonadotropins and estradiol levels in M were significantly enhanced while they were significantly decreased in CHM. The hypothalamic GnRH mRNA was also significantly increased in M and in CHM, as well as ERα mRNA. At the mean time, the hypothalamic GABAA receptor α1 and α3 subunits mRNA were more significantly decreased in M than those of N, while they were more significantly enhanced in CHM than those in M (p 0.01), the protein expression of which in hypothalamus had the same trend as the mRNA expression. The evidence suggests that Nourishing “Yin”-Removing “Fire” Chinese herb mixture could significantly retard the sexual development of the precocious rats, and up-regulate the expressions of hypothalamic GABAA receptor α1 and α3 subunits. Our result indicated that GABAA receptor α1 and α3 subunits might involve in the effective treatment of herb mixture on idiopathic precocious puberty.
文摘Combined acupuncture and Chinese herb decoction were applied for 5 cases with graphospasm.Acupuncture included scalp acupuncture and body acupuncture.Using transcranial repeated needling,Baihui(百会GV20),Chorea-trembling controlled area,Emotional area,as well as Dazhui(大椎GV14),Fengchi(风池GB20),and Yingxiang(迎香EX-HN8)etc.were stimulated by proposed by Sun Shentian.Meanwhile,the patients took orally Chinese herb decoction,on the basis of the Siwu decoction(四物汤four substances decoction)addition and subtraction,relieve symptoms under syndrome differentiation.The treatment was carried out for 4 courses in total.One course consisted of 5 days,with an interval of 2 days between two courses.After treatment of 5 patients,2 patients were cured,2 patients achieved markedly effective response,and 1 patient achieved effective response.
