A novel scaffold containing collagen-Ⅰ/polylactic acid(PLA)/nanohydroxyapatite(nHA) was prepared via co-electrospinning method.Different target substrates were used to improve the collection efficiency of this scaffo...A novel scaffold containing collagen-Ⅰ/polylactic acid(PLA)/nanohydroxyapatite(nHA) was prepared via co-electrospinning method.Different target substrates were used to improve the collection efficiency of this scaffold. The properties of the novel scaffold were compared with those of conventionally prepared ones.Compared to conventional method,the modified method was more efficient in producing the scaffold.Moreover,the porosity,thickness, and morphology of the novel scaffold were better than those of scaffolds prepared by conventional methods. The properties of collagen-Ⅰ,collagen-Ⅰ/PLA and collagen-Ⅰ/PLA/nHA scaffolds were also compared.Diameters of the electrospun fibers ranged from 180 to 405 nm,and roughness was present on the surface of the fibers due to the deposition of crystals of nHA along the long axis of the fibers.The fibers of the collagen-Ⅰ/PLA/nHA scaffold and the fibers of natural bone tissue had similar structure.展开更多
目的明确海洋生物来源的鱼I型胶原(marine type I collagen,MCI)对巨噬细胞生物学功能的影响,探讨其转化前景。方法诱导人源巨噬细胞,体外给予不同浓度MCI刺激,CCK8法观察MCI对巨噬细胞活性的影响;Transwell实验观察MCI对单核/巨噬细胞...目的明确海洋生物来源的鱼I型胶原(marine type I collagen,MCI)对巨噬细胞生物学功能的影响,探讨其转化前景。方法诱导人源巨噬细胞,体外给予不同浓度MCI刺激,CCK8法观察MCI对巨噬细胞活性的影响;Transwell实验观察MCI对单核/巨噬细胞细胞趋化能力的影响。免疫荧光实验和RT-qPCR观察MCI对巨噬细胞表型的影响。转录组测序观察MCI对巨噬细胞生物学功能的影响。结果MCI在1mg/ml和3mg/ml的浓度下,在体外不会对巨噬细胞的细胞活性、单核/巨噬细胞的趋化功能及巨噬细胞炎性表型产生显著影响。进一步转录组测序及验证发现,MCI可以显著提高巨噬细胞CXCL13的表达。结论鱼I型胶原可通过免疫调控促进巨噬细胞表达CXCL13,该现象可能利于骨缺损修复,提示该鱼I型胶原有一定的应用前景。展开更多
目的探讨血清I型胶原吡啶交联氨基末端肽(N-terminal telopeptide of type I collagen,NTX)联合I型胶原氨基端延长肽(Procollagen type I N-terminal propeptide,P1NP)对乳腺癌患者骨转移的诊断价值。方法选择2018年3月至2023年1月南通...目的探讨血清I型胶原吡啶交联氨基末端肽(N-terminal telopeptide of type I collagen,NTX)联合I型胶原氨基端延长肽(Procollagen type I N-terminal propeptide,P1NP)对乳腺癌患者骨转移的诊断价值。方法选择2018年3月至2023年1月南通市如皋中医院收治的女性乳腺癌骨转移患者72例(骨转移组)和单一乳腺癌患者72例(乳腺癌组),选择本院同期健康体检女性72例(健康组)。采用酶联免疫法检测并比较3组受试者血清NTX、P1NP表达水平的异同。分析血清NTX联合P1NP对乳腺癌患者骨转移的诊断价值,并采用Kappa检验分析血清指标诊断结果与临床诊断结果的一致性。结果骨转移组患者的血清NTX及P1NP水平高于乳腺癌组、健康组,乳腺癌组患者的血清NTX及P1NP水平高于健康组(P<0.05)。血清NTX、P1NP单一及联合诊断乳腺癌患者骨转移的曲线下面积(Area under curve,AUC)分别为0.794(95%CI:0.717~0.872)、0.789(95%CI:0.712~0.869)、0.875(95%CI:0.810~0.940),血清NTX、P1NP单一诊断乳腺癌患者骨转移的AUC均低于联合诊断(Z=3.971、4.025,P均<0.05)。血清NTX、P1NP单一及联合诊断乳腺癌患者骨转移与临床诊断结果一致性的Kappa值分别为0.597、0.556、0.750(P<0.05)。结论血清NTX、P1NP在乳腺癌骨转移中具有一定的辅助诊断价值,有助于早期甄别乳腺癌骨转移。展开更多
慢性肾脏病(chronic kidney disease,CKD)-矿物质和骨异常(mineral and bone disorder,MBD)引发的血管钙化(vascular calcification,VC)导致了CKD患者的高心血管死亡率,是CKD重要的临床并发症。骨标志物可观测或参与到VC的过程,对VC的...慢性肾脏病(chronic kidney disease,CKD)-矿物质和骨异常(mineral and bone disorder,MBD)引发的血管钙化(vascular calcification,VC)导致了CKD患者的高心血管死亡率,是CKD重要的临床并发症。骨标志物可观测或参与到VC的过程,对VC的防治起到重要作用。本文将对VC的发生机制进行深入学习,并对常见的骨标志物如甲状旁腺激素(PTH)-25羟维生素D(25-VitD)-成纤维细胞生长因子23(FGF23)-Klotho间的相互反馈影响、骨钙素(osteocalcin,OCN)、I型胶原羧基端肽β特殊序列(carboxy-terminal peptideβspecial sequence,β-CTX)、总Ⅰ型胶原氨基端延长肽(total type I collagen amino-terminal extension peptide,tP1NP)及研究进展做一综述。展开更多
基金Supported by the Scientific and Technological Developing Scheme of Jilin Province,China(No.20080302)the Fund of Jilin Provincial Science and Technology Department,China(No.200705115)
文摘A novel scaffold containing collagen-Ⅰ/polylactic acid(PLA)/nanohydroxyapatite(nHA) was prepared via co-electrospinning method.Different target substrates were used to improve the collection efficiency of this scaffold. The properties of the novel scaffold were compared with those of conventionally prepared ones.Compared to conventional method,the modified method was more efficient in producing the scaffold.Moreover,the porosity,thickness, and morphology of the novel scaffold were better than those of scaffolds prepared by conventional methods. The properties of collagen-Ⅰ,collagen-Ⅰ/PLA and collagen-Ⅰ/PLA/nHA scaffolds were also compared.Diameters of the electrospun fibers ranged from 180 to 405 nm,and roughness was present on the surface of the fibers due to the deposition of crystals of nHA along the long axis of the fibers.The fibers of the collagen-Ⅰ/PLA/nHA scaffold and the fibers of natural bone tissue had similar structure.
文摘目的明确海洋生物来源的鱼I型胶原(marine type I collagen,MCI)对巨噬细胞生物学功能的影响,探讨其转化前景。方法诱导人源巨噬细胞,体外给予不同浓度MCI刺激,CCK8法观察MCI对巨噬细胞活性的影响;Transwell实验观察MCI对单核/巨噬细胞细胞趋化能力的影响。免疫荧光实验和RT-qPCR观察MCI对巨噬细胞表型的影响。转录组测序观察MCI对巨噬细胞生物学功能的影响。结果MCI在1mg/ml和3mg/ml的浓度下,在体外不会对巨噬细胞的细胞活性、单核/巨噬细胞的趋化功能及巨噬细胞炎性表型产生显著影响。进一步转录组测序及验证发现,MCI可以显著提高巨噬细胞CXCL13的表达。结论鱼I型胶原可通过免疫调控促进巨噬细胞表达CXCL13,该现象可能利于骨缺损修复,提示该鱼I型胶原有一定的应用前景。
文摘目的探讨血清I型胶原吡啶交联氨基末端肽(N-terminal telopeptide of type I collagen,NTX)联合I型胶原氨基端延长肽(Procollagen type I N-terminal propeptide,P1NP)对乳腺癌患者骨转移的诊断价值。方法选择2018年3月至2023年1月南通市如皋中医院收治的女性乳腺癌骨转移患者72例(骨转移组)和单一乳腺癌患者72例(乳腺癌组),选择本院同期健康体检女性72例(健康组)。采用酶联免疫法检测并比较3组受试者血清NTX、P1NP表达水平的异同。分析血清NTX联合P1NP对乳腺癌患者骨转移的诊断价值,并采用Kappa检验分析血清指标诊断结果与临床诊断结果的一致性。结果骨转移组患者的血清NTX及P1NP水平高于乳腺癌组、健康组,乳腺癌组患者的血清NTX及P1NP水平高于健康组(P<0.05)。血清NTX、P1NP单一及联合诊断乳腺癌患者骨转移的曲线下面积(Area under curve,AUC)分别为0.794(95%CI:0.717~0.872)、0.789(95%CI:0.712~0.869)、0.875(95%CI:0.810~0.940),血清NTX、P1NP单一诊断乳腺癌患者骨转移的AUC均低于联合诊断(Z=3.971、4.025,P均<0.05)。血清NTX、P1NP单一及联合诊断乳腺癌患者骨转移与临床诊断结果一致性的Kappa值分别为0.597、0.556、0.750(P<0.05)。结论血清NTX、P1NP在乳腺癌骨转移中具有一定的辅助诊断价值,有助于早期甄别乳腺癌骨转移。
文摘慢性肾脏病(chronic kidney disease,CKD)-矿物质和骨异常(mineral and bone disorder,MBD)引发的血管钙化(vascular calcification,VC)导致了CKD患者的高心血管死亡率,是CKD重要的临床并发症。骨标志物可观测或参与到VC的过程,对VC的防治起到重要作用。本文将对VC的发生机制进行深入学习,并对常见的骨标志物如甲状旁腺激素(PTH)-25羟维生素D(25-VitD)-成纤维细胞生长因子23(FGF23)-Klotho间的相互反馈影响、骨钙素(osteocalcin,OCN)、I型胶原羧基端肽β特殊序列(carboxy-terminal peptideβspecial sequence,β-CTX)、总Ⅰ型胶原氨基端延长肽(total type I collagen amino-terminal extension peptide,tP1NP)及研究进展做一综述。
