Aim To study the chemical constituents from Coriolus versicolor L.. Methods Chromatographic techniques were employed for separation and purification. The structures were identified on the basis of spectroscopic analys...Aim To study the chemical constituents from Coriolus versicolor L.. Methods Chromatographic techniques were employed for separation and purification. The structures were identified on the basis of spectroscopic analysis. Results Eight compounds were isolated and identified as ergosta-7, 22-dien-313 -yl palmitate （1）, ergosta-7, 22-dien-313 -ol （2）, betulic acid （3）, 4-hydroxy benzoic acid （4）, 3- methoxy-4-hydroxy benzoic acid （5）, 3, 5-dimethoxyl-4-hydroxy benzoic acid （6）, 2-furoic acid （7）, and nicotinic acid （8）. Conclusion Eight compounds were reported for the first time from Coriolus versicolor L..展开更多
The capability of decolorization for commercial dyes by Coriolus versicolor fermentation broth containing laccase with or without immobilized mycelium was evaluated. With cell free fermentation broth containing l...The capability of decolorization for commercial dyes by Coriolus versicolor fermentation broth containing laccase with or without immobilized mycelium was evaluated. With cell free fermentation broth containing laccase, high decolorization ratio was achieved for acid orange 7, but not for the other dyes concerned. The immobilized mycelium was proved to be more efficient than the cell free system. All the four dyestuffs studied were found being decolourized with certain extent by immobilized mycelium. The repeated batch decolorization was carried out with satisfactory results. The experimental data showed that the continuous decolorization of wastewater from a printing and dyeing industry was possible by using the self immobilized C. Versicolor.展开更多
[ Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [ Method ] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, lac...[ Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [ Method ] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, laccase dosage on the decolorization of indigo dyeing wastewater by laccase/HBT, and then the synergism of laccase and acid cellulase was analyzed. [Result] Using ABTS as the substrate, the kinetic parame- ters, K,, and Vmax, were 0.318 mmol/L and 0.035 5 mmol/（ L . min） respectively. The decolorization rate of indigo reached 96.5% when the lacca- se acted on indigo for 40 min with HBT as an introducer at temperature 50 ℃, pH =4.5, indigo concentration 100 mg/L, HBT concentration 0.1% and laccase dosage 100 lU/L. Due to the synergism of laccase and acid cellulase during the bio-finishing of blue jeans, the backstaining degree of blue jeans reduced by 85% when the amount of laccase added was 15 000 IU/kg. Menawhile, the synergism of the laccase and acid cellulase de- creased indigo concentration in wastewater by 83.8%. [ Conclusion ] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.展开更多
The production of laccase by Coriolus versicolor was studied. The effect of cultivation conditions on laccase production by Coriolus versicolor was examined to obtain optimal medium and cultivation conditions....The production of laccase by Coriolus versicolor was studied. The effect of cultivation conditions on laccase production by Coriolus versicolor was examined to obtain optimal medium and cultivation conditions. Both batch and repeated batch processes were performed for laccase production. In repeated batch fermentation with self immobilized mycelia, total of 14 cycles were performed with laccase activity in the range between 3.4 and 14.8 U/ml.展开更多
Microbial transformation of diosgenin(1) was carried out with the white-rot fungus,Coriolus versicolor.A new polyhydroxyl metabolite,(25R)-spirost-5-ene-3β,7β,21-triol(2),was obtained as a result of hydroxylation.It...Microbial transformation of diosgenin(1) was carried out with the white-rot fungus,Coriolus versicolor.A new polyhydroxyl metabolite,(25R)-spirost-5-ene-3β,7β,21-triol(2),was obtained as a result of hydroxylation.Its structure was elucidated on the basis of 1D and 2D NMR as well as HR-ESI-MS spectroscopic analysis.展开更多
Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to reco...Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to recover hemolysin HC50, to increase the weight of the thymus, and increase the alexin of serum C3 and the IgG content of tumor bearing mice. FSP also significantly raised the pha-gocytic activity of macrophages in normal mice. PSP had a significant inhibitory effect on P38S and S180 cells. At the concentration of 1 mg/ml, PSP inhibited the proliferating activity of some human tumor call lines, such as SGC 7901, SPC, SLY and Mei. It had a direct toxic effect on SPC cells. PSP significantly inhibited the synthesis of nucleic acids of Ehrlich ascites carcinoma cells. In addition, PSP was antagonistic to the side effects of chemotherapy and radiotherapy.展开更多
Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biolog...Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.展开更多
Incidence of breast cancer is high in women worldwide and mortality usually results from tumor metastasis.Adjuvant chemotherapeutic,such as zoledronate(ZOL),has been used in metastatic breast cancer mainly for skeleta...Incidence of breast cancer is high in women worldwide and mortality usually results from tumor metastasis.Adjuvant chemotherapeutic,such as zoledronate(ZOL),has been used in metastatic breast cancer mainly for skeletal protection.Our previous studies demonstrated that Coriolus versicolor(CV)aqueous extract exhibited anti-tumor展开更多
Coriolus versicolor has demonstrated anti-cancer effects via polysaccharide-peptides(PSP)and polysaccharide Krestin(PSK).However,many other bioactive compounds within Coriolus versicolor(CV)may not have been identifie...Coriolus versicolor has demonstrated anti-cancer effects via polysaccharide-peptides(PSP)and polysaccharide Krestin(PSK).However,many other bioactive compounds within Coriolus versicolor(CV)may not have been identified.Our primary focus was to determine whether the ethanolic extract of Coriolus versicolor demonstrated any anti-cancer effects.The crude ethanolic extract was utilized,as was展开更多
The present study evaluated the potential of white-rot fungal strain Coriolus versicolor to decolorize five structurally different dyes in sequential batch reactors under optimized conditions. The experiments were run...The present study evaluated the potential of white-rot fungal strain Coriolus versicolor to decolorize five structurally different dyes in sequential batch reactors under optimized conditions. The experiments were run continuously for seven cycles of 8 d each. High decolorizing activity was observed even during the repeated reuse of the fungus, especially when the old medium was replaced with fresh medium after every cycle. Biodegradation was the dominating factor as the fungus was able to produce the enzyme laccase mainly, to mineralize synthetic dyes. The nutrients and composition of the medium played important roles in sustaining the decolorisation potential of the fungus. Corncob was found be an easy and cheap substitute for carbon source for the fungus. Glucose consumption by the fungus was in accordance to its decolorisation activity and chemical oxygen demand （COD） reduction.展开更多
基金Program for Changjiang Scholars and Innova-tive Team in University (985-2-063-112).
文摘Aim To study the chemical constituents from Coriolus versicolor L.. Methods Chromatographic techniques were employed for separation and purification. The structures were identified on the basis of spectroscopic analysis. Results Eight compounds were isolated and identified as ergosta-7, 22-dien-313 -yl palmitate （1）, ergosta-7, 22-dien-313 -ol （2）, betulic acid （3）, 4-hydroxy benzoic acid （4）, 3- methoxy-4-hydroxy benzoic acid （5）, 3, 5-dimethoxyl-4-hydroxy benzoic acid （6）, 2-furoic acid （7）, and nicotinic acid （8）. Conclusion Eight compounds were reported for the first time from Coriolus versicolor L..
基金TheNationalNaturalScienceFoundationofChina (No .2 9976 0 38)
文摘The capability of decolorization for commercial dyes by Coriolus versicolor fermentation broth containing laccase with or without immobilized mycelium was evaluated. With cell free fermentation broth containing laccase, high decolorization ratio was achieved for acid orange 7, but not for the other dyes concerned. The immobilized mycelium was proved to be more efficient than the cell free system. All the four dyestuffs studied were found being decolourized with certain extent by immobilized mycelium. The repeated batch decolorization was carried out with satisfactory results. The experimental data showed that the continuous decolorization of wastewater from a printing and dyeing industry was possible by using the self immobilized C. Versicolor.
基金Supported by the Foundation for Young Scholars of Educational Commission of Jiangxi Province,China (Foundation)National Natural Science Foundation of China (51064011)
文摘[ Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [ Method ] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, laccase dosage on the decolorization of indigo dyeing wastewater by laccase/HBT, and then the synergism of laccase and acid cellulase was analyzed. [Result] Using ABTS as the substrate, the kinetic parame- ters, K,, and Vmax, were 0.318 mmol/L and 0.035 5 mmol/（ L . min） respectively. The decolorization rate of indigo reached 96.5% when the lacca- se acted on indigo for 40 min with HBT as an introducer at temperature 50 ℃, pH =4.5, indigo concentration 100 mg/L, HBT concentration 0.1% and laccase dosage 100 lU/L. Due to the synergism of laccase and acid cellulase during the bio-finishing of blue jeans, the backstaining degree of blue jeans reduced by 85% when the amount of laccase added was 15 000 IU/kg. Menawhile, the synergism of the laccase and acid cellulase de- creased indigo concentration in wastewater by 83.8%. [ Conclusion ] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.
基金TheNationalNaturalScienceFoundationofChina (No .2 9976 0 38)
文摘The production of laccase by Coriolus versicolor was studied. The effect of cultivation conditions on laccase production by Coriolus versicolor was examined to obtain optimal medium and cultivation conditions. Both batch and repeated batch processes were performed for laccase production. In repeated batch fermentation with self immobilized mycelia, total of 14 cycles were performed with laccase activity in the range between 3.4 and 14.8 U/ml.
基金supported by the National Natural Science Foundation of China(No.30770237)the Program for New Century Excellent Talents in University(No.NCET-05-0852)
文摘Microbial transformation of diosgenin(1) was carried out with the white-rot fungus,Coriolus versicolor.A new polyhydroxyl metabolite,(25R)-spirost-5-ene-3β,7β,21-triol(2),was obtained as a result of hydroxylation.Its structure was elucidated on the basis of 1D and 2D NMR as well as HR-ESI-MS spectroscopic analysis.
文摘Polysocoharibe-peptide of Coriolus Versicolor (PSP) is a new anti-cancer immunomodulative drug. The present paper reports on the experimental research done with this drug. It was found that PSP had the ability to recover hemolysin HC50, to increase the weight of the thymus, and increase the alexin of serum C3 and the IgG content of tumor bearing mice. FSP also significantly raised the pha-gocytic activity of macrophages in normal mice. PSP had a significant inhibitory effect on P38S and S180 cells. At the concentration of 1 mg/ml, PSP inhibited the proliferating activity of some human tumor call lines, such as SGC 7901, SPC, SLY and Mei. It had a direct toxic effect on SPC cells. PSP significantly inhibited the synthesis of nucleic acids of Ehrlich ascites carcinoma cells. In addition, PSP was antagonistic to the side effects of chemotherapy and radiotherapy.
文摘Coriolus versicolor has been reported to have biological activities such as anticancer, antibacterial, antibiotics and immunopotentiation. The authors tried to ferment using lactobacilli associated with various biological functions and expected that fermented extract by lactobacilli will be superior to non-fermented extract in antioxidant activity. C. versicolor was extracted with 70% ethanol and then it was fermented by Lactobacillus acidophilus KCTC3164 and Lactobacillus plantarum KCTC3099, respectively. 1,l-dipheny｜-2-picrylhydrazyl （DPPH） scavenging activity, superoxide dismutase （SOD）-like activity and tetrazolium salt 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assays were performed. DPPH scavenging activity of fermented extract by L. acidophilus was higher than fermented extract by L. plantarum and antioxidant effect of fermented extract was 20%-30% higher than non-fermented extract. SOD-like activity was represented 18% in fermented extract by L. acidophilus, and was 27% in fermented extract by L. plantarum higher than non-fermented extract. As a result, this study suggests that it may need an efficient protocol for dominant bio-physiological activities such as extract volume or appropriate working volume of cultured cell or mixture condition. The fermented C. versicolor extract should provide with useful antioxidant agent in the human skin.
基金supported by Food and Health Bureau HKSAR,Health and Medical Research Fund no.10110891
文摘Incidence of breast cancer is high in women worldwide and mortality usually results from tumor metastasis.Adjuvant chemotherapeutic,such as zoledronate(ZOL),has been used in metastatic breast cancer mainly for skeletal protection.Our previous studies demonstrated that Coriolus versicolor(CV)aqueous extract exhibited anti-tumor
文摘Coriolus versicolor has demonstrated anti-cancer effects via polysaccharide-peptides(PSP)and polysaccharide Krestin(PSK).However,many other bioactive compounds within Coriolus versicolor(CV)may not have been identified.Our primary focus was to determine whether the ethanolic extract of Coriolus versicolor demonstrated any anti-cancer effects.The crude ethanolic extract was utilized,as was
基金the funding agencies, Department of Science and Technology, India and International Foundation for Science Sweden, for providing the financial support to conduct the studies reported in this article
文摘The present study evaluated the potential of white-rot fungal strain Coriolus versicolor to decolorize five structurally different dyes in sequential batch reactors under optimized conditions. The experiments were run continuously for seven cycles of 8 d each. High decolorizing activity was observed even during the repeated reuse of the fungus, especially when the old medium was replaced with fresh medium after every cycle. Biodegradation was the dominating factor as the fungus was able to produce the enzyme laccase mainly, to mineralize synthetic dyes. The nutrients and composition of the medium played important roles in sustaining the decolorisation potential of the fungus. Corncob was found be an easy and cheap substitute for carbon source for the fungus. Glucose consumption by the fungus was in accordance to its decolorisation activity and chemical oxygen demand （COD） reduction.
