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Effect of topical 0.05% cyclosporine A on corneal endothelium in patients with dry eye disease 被引量:6
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作者 Consuelo Pérez-Rico Francisco Germain +2 位作者 María Castro-Rebollo Agustín Moreno-Salgueiro Miguel ngel Teus 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2013年第4期471-474,共4页
· AIM: To determine the effect of topical 0.05% cyclosporine A (CsA) on corneal endothelium in patients with dry eye disease. · METHODS: Observational, prospective, case series study. Fifty-five eyes of 29 c... · AIM: To determine the effect of topical 0.05% cyclosporine A (CsA) on corneal endothelium in patients with dry eye disease. · METHODS: Observational, prospective, case series study. Fifty-five eyes of 29 consecutive patients (9 males and 20 females; median age: 66.8 years, interquartile range: 61 -73.2 years) with moderate -severe dry eye disease were evaluated. All patients were treated with topical 0.05% CsA ophthalmic emulsion twice a day in addition to lubricant eyedrops 5 times a day. The follow- up period was 12 months. Before treatment and at 3 and 12 months post -treatment central corneal specular microscopy was performed. The endothelial cell density (ECD), coefficient of variation of cell size (CoV), and percentage of hexagonal cells (Hex %) were analyzed. ·RESULTS: The median ECDs pre-treatment and at 3 and 12 months post-treatment were 2 352.5/mm 2 (inter- quartile range, 2 178 -2548.5), 2 364/mm 2 (interquartile range, 2 174.25 -2 657.5), and 2 366 cells/mm 2 (inter - quartile range, 2 174.75-2 539.75), respectively (P=0.927, one way ANOVA). The median CoVs pre-treatment and at 3 and 12 months post -treatment were 34.5 (interquartile range, 30 -37), 35 (interquartile range, 30 -38), and 34 (interquartile range, 30.75-38.25), respectively (P=0.7193, one way ANOVA). The median Hex % values pre - treatment and at 3 and 12 months post -treatment were 53 (interquartile range, 47 -58), 54 (interquartile range, 45.75 -59), and 50.5 (interquartile range, 45.75 -58), respectively (P=0.824, one way ANOVA). · CONCLUSION: Treatment of patients with dry eye disease for 12 months with topical 0.05% CsA does not seem to cause substantial changes on corneal endothelium. 展开更多
关键词 corneal endothelium corneal toxicity topical 0.05% cyclosporine A dry eye disease specular microscopy
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Differences in energy and corneal endothelium between femtosecond laser-assisted and conventional cataract surgeries:prospective,intraindividual,randomized controlled trial 被引量:6
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作者 Lucia Bascaran Txomin Alberdi +2 位作者 Itziar Martinez-Soroa Cristina Sarasqueta Javler Mendlcute 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第8期1308-1316,共9页
AIM: To compare intraoperative phacoemulsification parameters and its effect on the corneal endothelium of eyes undergoing femtosecond laser-assisted cataract surgery(FLACS) versus conventional phacoemulsification... AIM: To compare intraoperative phacoemulsification parameters and its effect on the corneal endothelium of eyes undergoing femtosecond laser-assisted cataract surgery(FLACS) versus conventional phacoemulsification(CP) cataract surgery.METHODS: Two hundred eyes from one hundred patients were included in a prospective, non-blinded, randomized, controlled, intraindividual clinical study. One hundred eyes underwent FLACS while their one hundred fellow eyes underwent CP. All surgeries were performed using the Victus? femtosecond laser platform and Infinity? Vision System phacoemulsification machine. Primary outcome measure was endothelial cell density 6 mo after surgery. Secondary outcome measures included central corneal thickness(CCT), average cell area, standard deviation, coefficient of variation and hexagonality before surgery and 6 mo after surgery and endothelial cell density loss during this period were also evaluated. Intraoperative efficiency parameters [cumulative dissipated energy(CDE), total intraocular surgery time, total ultrasound time, total phacoemulsification time, total torsional energy time, total aspiration time, ultrasound energy, torsional amplitude and fluid required during surgery] were also collated. RESULTS: Data from these patients was not considered for analysis. Data from 92 patients were analysed. Postoperative endothelial cell density(cells/mm2) between groups(2211.88±392.49 CP; 2246.31±403.48 FLACS) was not statistically significant(P=0.869). Total ultrasound time, torsional energy time, CDE and fluid requirements were significantly lower the FLACS group(P〈0.05). Other parameters did not show statistically significant difference between FLACS and CP.CONCLUSION: FLACS displays significant improvements in phacoemulsification parameters in comparison to CP. There are no significant differences in corneal endothelium measures between FLACS and CP. 展开更多
关键词 CATARACT femtosecond laser assisted cataractsurgery phacoemulsification parameters corneal endothelium endothelial cell morphology
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EXPERIMENTAL STUDY ON THE CORNEAL ENDOTHELIUM OF TRAUMATIC CATARACT 被引量:1
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作者 Quanjin Xie Yunliang Wang Miaorong Shao Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060, China 《眼科学报》 1991年第3期156-160,共5页
The cell morphology of corneal endothelium in 84 mice with experimental traumatic cataract was investigated with stained corneal buttons. In the experimental group, the boundaries between adjacent corneal endothelial ... The cell morphology of corneal endothelium in 84 mice with experimental traumatic cataract was investigated with stained corneal buttons. In the experimental group, the boundaries between adjacent corneal endothelial cells were significantly distorted, some cell boundaries manifested degenerative changes that led to coalescence of the cells. The mean density and mean area of endothelial cells of the controls showed significant difference from those of the experimental group during the 12 weeks of observ... 展开更多
关键词 EXPERIMENTAL STUDY ON THE corneal endothelium OF TRAUMATIC CATARACT THAN
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Hammered silver appearance of the corneal endothelium in Fuchs uveitis syndrome:A case report
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作者 Yan-Yan Cheng Cong-Ying Wang +1 位作者 Yan-Fang Zheng Ming-Yu Ren 《World Journal of Clinical Cases》 SCIE 2022年第35期13081-13087,共7页
BACKGROUND Hammered silver appearance of the corneal endothelium is considered a characteristic change in iridocorneal-endothelial syndrome.Herein we report an interesting case of hammered silver appearance of the cor... BACKGROUND Hammered silver appearance of the corneal endothelium is considered a characteristic change in iridocorneal-endothelial syndrome.Herein we report an interesting case of hammered silver appearance of the corneal endothelium in Fuchs uveitis syndrome(FUS).CASE SUMMARY A 49-year-old man with progressive vision loss in the right eye for one year was admitted to our hospital.The clinical manifestations of the patient’s right eye were mild conjunctival hyperemia,scattered stellate keratic precipitates on the corneal endothelium,normal depth anterior chamber,2+cellular reaction in the aqueous humor,diffuse iris depigmentation,absence of synechia,Koeppe nodules,opalescent lens,and vitreous opacity.FUS and a complicated cataract were diagnosed based on the typical clinical manifestations.The corneal endothelial changes were recorded in detail by slit-lamp examination,specular microscopy,and in vivo confocal microscopy before cataract extraction,revealing a hammered silver appearance of the corneal endothelium in the affected eye,a wide-band dark area,as well as irregular corneal endothelial protuberances and dark bodies of various sizes.Subsequently,the patient underwent phacoemulsification combined with intraocular lens implantation,and his postoperative visual acuity recovered to 1.0.CONCLUSION Hammered silver appearance of the corneal endothelium in FUS,which is considered a more serious manifestation of endothelial damage,is rare and may be caused by many irregular protrusions in the corneal endothelium. 展开更多
关键词 Fuchs uveitis syndrome corneal endothelium Hammered silver Case report
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Evaluation of corneal endothelium after UVA/riboflavin cross-linking in thin keratoconic corneas
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作者 Wei Chen Zhi-Wei Li +2 位作者 Xiao-Min Zhao Wen -Wen Xu Guo-Ying Mu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第2期321-322,共2页
Dear Sir,UVA/riboflavin cross-linking (CXL) has been used clinically applied for the treatment of keratoconus and corneal edema via enhancement of corneal stiffness The safety of the corneal endothelium is of prime... Dear Sir,UVA/riboflavin cross-linking (CXL) has been used clinically applied for the treatment of keratoconus and corneal edema via enhancement of corneal stiffness The safety of the corneal endothelium is of prime importance during CXL treatment. In clinical practice, a corneal thickness (CT) of 400um has traditionally been regarded as the minimum treatable thickness, thereby avoiding damage to the corneal endothelium Although CXL has been applied to thinner corneas, using a hypoosmotic solution onto cornea and inducing edema . CXL safety still needs further evaluation because of lower relative concentration of collagen in the hydrated stroma . This study aims to evaluate the changes of corneal endothelial density (ECD) in cases where the CT is 〈400 um before iatrogenic corneal swelling and CXL treatment. 展开更多
关键词 UVA Evaluation of corneal endothelium after UVA/riboflavin cross-linking in thin keratoconic corneas
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Short term effects of small incision lenticule extraction surgery on corneal endothelium
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作者 Dan-Yang Wang Man-Li Liu +6 位作者 Yi-Le Chen Xiao-Ying Zhang Yang-Tao Xu Jian-Chao Wang Chi-Ho To Jian-Guo Wang Quan Liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第4期536-539,共4页
AIM: To assess the effects of small incision lenticule extraction(SMILE) surgery on the corneal endothelium at1 d to 1mo postoperatively.·METHODS: A retrospective, observational study was conducted on 47 pati... AIM: To assess the effects of small incision lenticule extraction(SMILE) surgery on the corneal endothelium at1 d to 1mo postoperatively.·METHODS: A retrospective, observational study was conducted on 47 patients(47 eyes) who received SMILE surgery. Patients were grouped according to contact lens wear condition. The corneal endothelium was examined preoperatively and at 1d, 1wk and 1mo postoperatively.The corneal endothelium was analyzed for endothelial cell density(ECD), percentage of hexagonal cells, and coefficient of variation(CV) of cell size.·RESULTS: There were no significant decrease in the ECD, percentage of hexagonal cells or increase in CV at1 d, 1wk and 1mo postoperatively(P 〉0.05). However,there was a small increase of ECD by 2.88% in contact lens wearers(78.26±113.62 cell/mm2, P 〈0.05).· CONCLUSION: SMILE has no significant adverse effects on the corneal ECD and morphology during 1mo follow-up time. 展开更多
关键词 myopia corneal endothelium refractive surgery
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AB086.Influence of the intraocular pressure on the expression of tight junctions in the corneal endothelium
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作者 Mathieu Thériault Olivier Roy +1 位作者 Isabelle Brunette Stéphanie Proulx 《Annals of Eye Science》 2018年第1期492-492,共1页
Background:Cells are influenced by their environment.In vivo,the corneal endothelium is subjected to intraocular pressure(IOP).The purpose of this project was to evaluate in vitro,the effect of the IOP on the formatio... Background:Cells are influenced by their environment.In vivo,the corneal endothelium is subjected to intraocular pressure(IOP).The purpose of this project was to evaluate in vitro,the effect of the IOP on the formation of tight junctions in the corneal endothelium.Methods:Cultivated corneal endothelial cells(P2-P3;n=6 populations)were seeded on devitalized on corneas(n=10 pairs).Native corneas and devitalized corneas were respectively used as positive(n=2 pairs)and negative controls(n=3 pairs).Corneas were placed in artificial anterior chambers and subjected to a hydrostatic pressure between 0.3 and 0.4 psi during 4-5 days.Unpressured control corneas were maintained in cell culture dishes.Pictures of the corneas were taken following the experiment to assess stromal transparency.Morphology,corneal thickness and distribution of ZO-1,n-cadherin,b-catenin,NaK ATPase pump and HCO3-cotransporter were evaluated by electron microscopy,histological staining and immunofluorescences.Results:Pressure treated corneas were more transparent than the controls.Thickness was accordingly reduced by 38.4%±4.9%for cultivated endothelium and 32.2%±2.7%for native endothelium.Negative controls change in transparency and thickness were marginal.Pressure treated cells showed none or at most marginal difference in morphology and expression of ZO-1,n-cadherin,b-catenin,NaK ATPase pump and HCO3-cotransporters and failed to recreate a phenotype similar to native corneas.Pressure however increased cortical localisation of the protein ZO-1 in both cultivated and native endothelium.Conclusions:These results suggest that anterior chamber hydrostatic pressure may enhance endothelial functionality by modulating the distribution of tight junction’s proteins. 展开更多
关键词 corneal endothelium intraocular pressure(IOP) tissue engineering tight junctions
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The role of corneal endothelium in macular corneal dystrophy development and recurrence
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作者 Bi-Ning Zhang Benxiang Qi +13 位作者 Chunxiao Dong Bin Zhang Jun Cheng Xin Wang Suxia Li Xiaoyun Zhuang Shijiu Chen Haoyun Duan Dewei Li Sujie Zhu Guoyun Li Yihai Cao Qingjun Zhou Lixin Xie 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第2期332-344,共13页
Macular corneal dystrophy(MCD)is a progressive,bilateral stromal dystrophic disease that arises from mutations in carbohydrate sulfotransferase 6(CHST6).Corneal transplantation is the ultimate therapeutic solution for... Macular corneal dystrophy(MCD)is a progressive,bilateral stromal dystrophic disease that arises from mutations in carbohydrate sulfotransferase 6(CHST6).Corneal transplantation is the ultimate therapeutic solution for MCD patients.Unfortunately,postoperative recurrence remains a significant challenge.We conducted a retrospective review of a clinical cohort comprising 102 MCD patients with 124 eyes that underwent either penetrating keratoplasty(PKP)or deep anterior lamellar keratoplasty(DALK).Our results revealed that the recurrence rate was nearly three times higher in the DALK group(39.13%,9/23 eyes)compared with the PKP group(10.89%,11/101 eyes),suggesting that surgical replacement of the corneal endothelium for treating MCD is advisable to prevent postoperative recurrence.Our experimental data confirmed the robust m RNA and protein expression of CHST6 in human corneal endothelium and the rodent homolog CHST5 in mouse endothelium.Selective knockdown of wild-type Chst5 in mouse corneal endothelium(AC^(siChst5)),but not in the corneal stroma,induced experimental MCD with similar extracellular matrix synthesis impairments and corneal thinning as observed in MCD patients.Mice carrying Chst5 point mutation also recapitulated clinical phenotypes of MCD,along with corneal endothelial abnormalities.Intracameral injection of wild-type Chst5 rescued the corneal impairments in AC^(siChst5)mice and retarded the disease progression in Chst5 mutant mice.Overall,our study provides new mechanistic insights and therapeutic approaches for MCD treatment by highlighting the role of corneal endothelium in MCD development. 展开更多
关键词 macular corneal dystrophy RECURRENCE corneal endothelium keratan sulfate penetrating keratoplasty
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Effect of mitomycin C on the corneal endothelial cells of Saudi patients with myopia after transepithelial photorefractive keratectomy:a two-armed cohort study
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作者 Sultan H Alrashidi 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第5期909-915,共7页
AIM:To study the effect of mitomycin C(MMC)applied during transepithelial photorefractive keratectomy(TPRK)on the corneal endothelium one week(W1)and three months(M3)after surgery and its determinants.METHODS:In this ... AIM:To study the effect of mitomycin C(MMC)applied during transepithelial photorefractive keratectomy(TPRK)on the corneal endothelium one week(W1)and three months(M3)after surgery and its determinants.METHODS:In this two-armed cohort study conducted in 2022,eyes treated with MMC during TPRK(group 1)were compared with eyes not treated with MMC(group 2).The corneal endothelial cell(EC)count,EC density(ECD;cells/mm2),average(μm2),standard deviation(μm2),coefficient of variation(CV%),ECmax,ECmin,and EC percentage of hexagonality were estimated at W1 and M3.The postoperative changes in the EC count in the two groups were compared and correlated with the other independent variables.RESULTS:Group 1 had 26 eyes,and group 2 had 78 eyes.All TPRK indices were significantly higher for the eyes in group 1 than for those in group 2.The MMC usage was not a significant predictor of the change in ECD(P=0.644),change in CV(P=0.374),and change in the percentage of hexagonality of EC(P=0.164)at W1.However,the use of MMC was a significant predictor of changes in CV(P=0.014)and the change in the percentage of hexagonality of EC(P=0.039)at M3.The duration of laser exposure and the size of the optical zone influenced the correlation of MMC use with the changes in EC indices,postoperatively.CONCLUSION:The use of MMC doesn’t affect ECD,CV,and percentage of hexagonality at W1 if other surgical indices are considered.At M3 after operating myopic eyes by TPRK,MMC significantly influence the CV and percentage of hexagonality.The duration of the laser application and the size of the optical zone should be considered when determining the effect of MMC on the EC indices. 展开更多
关键词 transepithelial photorefractive keratectomy MITOMYCIN-C corneal endothelium
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Therapeutic efficiency of tissue-engineered human corneal endothelium transplants on rabbit primary corneal endotheliopathy 被引量:3
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作者 Ting-jun FAN Jun ZHAO +3 位作者 Xiu-zhong HU Xi-ya MA Wen-bo ZHANG Chao-zhong YANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2011年第6期492-498,共7页
To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP),TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mc... To evaluate the therapeutic efficiency of tissue-engineered human corneal endothelia (TE-HCEs) on rabbit primary corneal endotheliopathy (PCEP),TE-HCEs reconstructed with monoclonal human corneal endothelial cells (mcHCECs) and modified denuded amniotic membranes (mdAMs) were transplanted into PCEP models of New Zealand white rabbits using penetrating keratoplasty.The TE-HCEs were examined using diverse techniques including slit-lamp biomicroscopy observation and pachymeter and tonometer measurements in vivo,and fluorescent microscopy,alizarin red staining,paraffin sectioning,scanning and transmission electron microscopy observations in vitro.The corneas of transplanted eyes maintained transparency for as long as 200 d without obvious edema or immune rejection.The corneal thickness of transplanted eyes decreased gradually after transplanting,reaching almost the thickness of normal eyes after 156 d,while the TE-HCE non-transplanted eyes were turbid and showed obvious corneal edema.The polygonal corneal endothelial cells in the transplanted area originated from the TE-HCE transplant.An intact monolayer corneal endothelium had been reconstructed with the morphology,cell density and structure similar to those of normal rabbit corneal endothelium.In conclusion,the transplanted TE-HCE can reconstruct the integrality of corneal endothelium and restore corneal transparency and thickness in PCEP rabbits.The TE-HCE functions normally as an endothelial barrier and pump and promises to be an equivalent of HCE for clinical therapy of human PCEP. 展开更多
关键词 Tissue-engineered human corneal endothelium New Zealand white rabbits Penetrating keratoplasty Primary corneal endotheliopathy TRANSPARENCY
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Design of functional biomaterials as substrates for corneal endothelium tissue engineering
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作者 Begona M.Bosch Elia Bosch-Rue +1 位作者 Marina Perpiñan-Blasco Roman A.Perez 《Regenerative Biomaterials》 SCIE EI 2022年第1期1018-1036,共19页
Corneal endothelium defects are one of the leading causes of blindness worldwide.The actual treatment is transplantation,which requires the use of human cadaveric donors,but it faces several problems,such as global sh... Corneal endothelium defects are one of the leading causes of blindness worldwide.The actual treatment is transplantation,which requires the use of human cadaveric donors,but it faces several problems,such as global shortage of donors.Therefore,new alternatives are being developed and,among them,cell therapy has gained interest in the last years due to its promising results in tissue regeneration.Nevertheless,the direct administration of cells may sometimes have limited success due to the immune response,hence requiring the combination with extracellular mimicking materials.In this review,we present different methods to obtain corneal endothelial cells from diverse cell sources such as pluripotent or multipotent stem cells.Moreover,we discuss different substrates in order to allow a correct implantation as a cell sheet and to promote an enhanced cell behavior.For this reason,natural or synthetic matrixes that mimic the native environment have been developed.These matrixes have been optimized in terms of their physicochemical properties,such as stiffness,topography,composition and transparency.To further enhance the matrixes properties,these can be tuned by incorporating certain molecules that can be delivered in a sustained manner in order to enhance biological behavior.Finally,we elucidate future directions for corneal endothelial regeneration,such as 3D printing,in order to obtain patient-specific substrates. 展开更多
关键词 corneal endothelium tissue engineering cell therapy BIOMATERIALS
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1.8mm角膜微切口与3.0mm角膜切口同轴白内障超声乳化术对角膜内皮及术源性散光的对照观察
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作者 程武波 朱旭果 +5 位作者 吴桂花 刘雪莲 张燕 宋秋平 蒋红 罗银星 《实用防盲技术》 2024年第2期53-57,F0002,共6页
目的观察比较白内障超声乳化术1.8mm角膜切口与3.0mm角膜切口对角膜内皮和术源性散光的影响。方法根据不同术式将120例白内障患者随机分为1.8mm切口观察组与3.0mm切口对照组行预劈核超声乳化治疗,观察两组病例平均超声时间,平均超声能量... 目的观察比较白内障超声乳化术1.8mm角膜切口与3.0mm角膜切口对角膜内皮和术源性散光的影响。方法根据不同术式将120例白内障患者随机分为1.8mm切口观察组与3.0mm切口对照组行预劈核超声乳化治疗,观察两组病例平均超声时间,平均超声能量,术前,第1d,1wk,1mo的视力、角膜散光变化、角膜内皮计数等情况。结果观察组和对照组有效超乳时间9.51±1.17,9.48±1.21及平均超乳能量(10.01±1.02)%(9.9±1.19)%比较差异均无统计学意义(P>0.05)。两组术前视力0.11±0.057,0.10±0.012比较无统计学差异(P>0.05),术后第1d两组视力0.376±0.071,0.319±0.14比较有统计学意义(P<0.05);术后1wk;1mo两组视力0.410±0.14,0.356±0.12,0.429±0.15,0.364±0.14比较有统计学意义(P<0.05)。平均角膜散光两组术前0.59±0.25,0.61±0.30比较无统计学意义(P>0.05),术后两组第1d(0.91±0.42,1.15±0.41),1wk(0.76±0.26,0.95±0.24)和1mo(0.66±0.23,0.74±0.25)比较有统计学差异(P<0.05)。角膜内皮计数两组术前2753.32±324.12,2645.36±321.45比较无统计学意义(P>0.05),术后第1d(2412.36±541.12,2397.62±204.21),1wk(2365.35±233.72,2351.36±248.63)和1mo(2298.37±251.89,2209.45±373.07),比较无统计学差异(P>0.05)。结论,1.8mm切口超声乳化术同传统3.0mm切口组比较,不导致超乳效率下降及更多的角膜内皮细胞的损伤,且术后医源性散光相较更小,术后视力更佳。 展开更多
关键词 白内障超声乳化术 微切口 角膜内皮损伤 术源性散光
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人工晶状体保护下超声乳化白内障吸除术疗效及安全性评价:一项随机对照临床研究
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作者 金玉娇 李楠 +6 位作者 孙秋爽 金韦奕 江美玲 刘颖锋 卢焱 孙丽霞 崔仁哲 《中华实验眼科杂志》 CAS CSCD 北大核心 2024年第3期248-255,共8页
目的观察人工晶状体(IOL)保护下超声乳化手术(PHACO)对硬核白内障患者的治疗效果。方法采用随机对照临床研究方法,连续纳入2019年1月至2022年5月于延边大学附属医院收治的Emery分级Ⅳ~Ⅴ级的硬核白内障患者120例120眼,按照随机数字表法... 目的观察人工晶状体(IOL)保护下超声乳化手术(PHACO)对硬核白内障患者的治疗效果。方法采用随机对照临床研究方法,连续纳入2019年1月至2022年5月于延边大学附属医院收治的Emery分级Ⅳ~Ⅴ级的硬核白内障患者120例120眼,按照随机数字表法分为PHACO组、IOL保护PHACO组和囊外白内障摘除术(ECCE)组,分别行常规PHACO、IOL保护下的PHACO和ECCE,每组40例40眼。最终99例受试者完成随访,其中PHACO组30例30眼,IOL保护PHACO组35例35眼,ECCE组34例34眼。记录各组总手术时间、术中超声乳化时间、累计释放能量。术后随访3个月,检测并比较3个组患眼角膜内皮细胞密度(ECD)、角膜内皮细胞面积变异系数(CV)、角膜六角形内皮细胞比率(6A)、角膜散光值,不同等级裸眼远视力的眼数分布;记录术中和术后并发症发生情况。结果IOL保护PHACO组与PHACO组超声能量及超声时间比较差异均无统计学意义(P=0.691、0.982)。IOL保护PHACO组和PHACO组总手术时间分别为(38.81±2.73)和(36.45±3.45)min,明显短于ECCE组的(69.60±4.35)min,差异均有统计学意义(均P<0.001)。术前3个组患者年龄、性别、晶状体核硬度等基本资料比较,差异均无统计学意义(均P>0.05)。术后3个月,PHACO组和IOL保护PHACO组裸眼视力较高的人数较ECCE组高(P=0.006、0.007);IOL保护PHACO组的ECD和6A分别为(2155.57±177.88)/mm^(2)和(41.31±5.18)%,均显著高于PHACO组的(1912.64±224.11)/mm^(2)和(36.18±3.27)%,IOL保护PHACO组的CV为(50.34±5.90)%,低于PHACO组的(55.67±3.30)%,差异均有统计学意义(P=0.007、0.003、0.005)。术后1周和3个月,IOL保护PHACO组的角膜散光度数均显著低于ECCE组,且高于PHACO组,差异均有统计学意义(均P<0.05)。结论IOL保护下的PHACO相对常规PHACO可有效减少超声能量对角膜内皮的损伤,相对ECCE能明显缩短手术时间,减轻术后炎症反应,且并未明显增加术后角膜散光。IOL保护下的PHACO是硬核白内障患者一种有效的改良手术方式。 展开更多
关键词 白内障 人工晶状体 超声乳化白内障吸除术 角膜内皮 术源性散光
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角膜内皮移植研究热点分析及未来研究方向预测 被引量:1
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作者 郭钰 张弘 《中华实验眼科杂志》 CAS CSCD 北大核心 2024年第2期136-142,共7页
目的了解角膜内皮移植(EK)近年的文献分布规律和研究热点。方法以Web of Science核心合集中的科学引文扩展索引和社会科学引文索引为数据源,提取2011—2021年以EK为主题的文献,对以EK为主题、文献类型为论著的年文献量、文献发表量前10... 目的了解角膜内皮移植(EK)近年的文献分布规律和研究热点。方法以Web of Science核心合集中的科学引文扩展索引和社会科学引文索引为数据源,提取2011—2021年以EK为主题的文献,对以EK为主题、文献类型为论著的年文献量、文献发表量前10的作者和期刊、引用频次前10位的论文及其主要观点进行统计,并将文献数据导入VOSviewer软件,对EK相关领域文献的核心作者及关键词进行可视化分析。结果共检索出文献1454篇。2011—2021年,文献发表量的逐年累积量呈线性增长,美国是发表EK相关文献最多的国家,Melles GRJ的论文发表量和论文被引数均占据第1位,文献发表总量排名第1的期刊为Cornea。目前,EK的研究方向主要集中于多种术式术后效果的比较和相关并发症的处理。关键词分析显示,其被分为5个聚类,其中聚类1是关于EK术后视觉质量的评价;聚类2是术后并发症和移植失败高风险因素的分析;聚类3是以角膜后弹力层内皮移植(DMEK)为中心的适应证和手术技巧的研究;聚类4是植片相关问题,如植片制备、内皮细胞损失、术后植片脱位的处理等;聚类5主要是角膜移植术式的变化趋势。出现频次最多的关键词是DMEK,共559次,说明目前DMEK是EK相关研究领域的重点。2017年以来,"DMEK"、"previous trabeculectomy"、"graft preparation"、"stability"、"safety"、"air"等关键词出现频次增加,可见有青光眼手术史的病例处理、手术的安全性和稳定性、大气泡法制备植片是近几年来的研究热点。结论植片制备技巧、复杂病例处理、角膜内皮疾病患者青光眼的治疗方法、大气泡法制备植片的长期术后效果评估可能成为今后EK研究发展的方向。 展开更多
关键词 角膜移植术 角膜内皮 数据可视化 文献计量学
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Profile of biological characterizations and clinical application of corneal stem/progenitor cells 被引量:2
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作者 Pei-Xi Ying Min Fu +11 位作者 Chang Huang Zhi-Hong Li Qing-Yi Mao Sheng Fu Xu-Hui Jia Yu-Chen Cao Li-Bing Hong Li-Yang Cai Xi Guo Ru-Bing Liu Fan-ke Meng Guo-Guo Yi 《World Journal of Stem Cells》 SCIE 2022年第11期777-797,共21页
Corneal stem/progenitor cells are typical adult stem/progenitor cells.The human cornea covers the front of the eyeball,which protects the eye from the outside environment while allowing vision.The location and functio... Corneal stem/progenitor cells are typical adult stem/progenitor cells.The human cornea covers the front of the eyeball,which protects the eye from the outside environment while allowing vision.The location and function demand the cornea to maintain its transparency and to continuously renew its epithelial surface by replacing injured or aged cells through a rapid turnover process in which corneal stem/progenitor cells play an important role.Corneal stem/progenitor cells include mainly corneal epithelial stem cells,corneal endothelial cell progenitors and corneal stromal stem cells.Since the discovery of corneal epithelial stem cells(also known as limbal stem cells)in 1971,an increasing number of markers for corneal stem/progenitor cells have been proposed,but there is no consensus regarding the definitive markers for them.Therefore,the identification,isolation and cultivation of these cells remain challenging without a unified approach.In this review,we systematically introduce the profile of biological characterizations,such as anatomy,characteristics,isolation,cultivation and molecular markers,and clinical applications of the three categories of corneal stem/progenitor cells. 展开更多
关键词 corneal epithelial stem cells corneal endothelium stem cells corneal stromal stem cells BIOENGINEERING Gene markers
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The Study on Improved Cryopreservation Technique of The Ultrastructure of Corneal Endothelial Cells 被引量:1
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作者 Zhiyang Jia, Shujin Liang, Changling Wang , Peixin ZhugeDepartment of Ophthalmology, The Second Affiliated Hospital of Hebei Medical College Shijiazhuang 050000, China.Laboratory of Eye Disease, The Second Affiliated Hospital of Hebei Medical college Shijiazhuang 050000, China 《眼科学报》 1994年第1期13-20,共8页
The traditional corneal cryopreservation technique was improved. We carried out an experimental study that rabbit corneas were cryop-reserved by using polyvinylpyrolidone (PVP) as cryoprotective agent and dimethlsulfo... The traditional corneal cryopreservation technique was improved. We carried out an experimental study that rabbit corneas were cryop-reserved by using polyvinylpyrolidone (PVP) as cryoprotective agent and dimethlsulfoxide (DMSO) as the control. The endothelia of cryopreserved corneas were evaluated by scanning and transmission electron microscopy and vital staining. The study shows that PVP is an excellent extracellular cryoprotective agent and has the characteristic of low toxicity or no toxicity to co... 展开更多
关键词 corneal endothelium ULTRASTRUCTURE CRYOPRESERVATION
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组织工程角膜内皮移植研究进展
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作者 贾艳妮(综述) 周庆军(审校) 史伟云(审校) 《中华实验眼科杂志》 CAS CSCD 北大核心 2024年第2期192-197,共6页
由于角膜供体材料严重短缺,穿透角膜移植术及角膜内皮移植术的临床广泛开展受到严重制约,其根本原因在于健康角膜内皮的增生能力有限。随着组织工程技术和细胞工程技术不断发展,组织工程角膜研究已取得一定进展,应用组织工程技术体外培... 由于角膜供体材料严重短缺,穿透角膜移植术及角膜内皮移植术的临床广泛开展受到严重制约,其根本原因在于健康角膜内皮的增生能力有限。随着组织工程技术和细胞工程技术不断发展,组织工程角膜研究已取得一定进展,应用组织工程技术体外培养高密度、具备健康内皮功能的角膜内皮细胞进行移植是当前研究的热点。组织工程角膜内皮技术研发的关键在于种子细胞、载体材料和移植方式的选择。目前,国内外大量研究的种子细胞来源包括人角膜内皮细胞、干细胞、血管内皮细胞及人羊膜上皮细胞等。常见的载体材料包括羊膜、脱细胞角膜基质、后弹力层、晶状体前囊膜等。体外培养的细胞采用穿透角膜移植术、角膜内皮移植术或前房注射细胞的方式进行移植。本文从角膜内皮种子细胞来源、移植载体选择以及角膜内皮移植方法等方面就组织工程角膜内皮移植研究进展进行综述,总结目前研究面临的问题并展望其前景。 展开更多
关键词 角膜内皮 移植 组织工程 种子细胞 载体
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Research progress on the negative factors of corneal endothelial cells proliferation
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作者 Yu-Bo Cui Jing Wu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2012年第5期614-619,共6页
The human corneal endothelium forms a boundary layer between anterior chamber and corneal stoma. The corneal endothelial cells are responsible for maintaining cornea transparency, which is very vital for our visual ac... The human corneal endothelium forms a boundary layer between anterior chamber and corneal stoma. The corneal endothelial cells are responsible for maintaining cornea transparency, which is very vital for our visual acuity, via its pump and barrier functions. The adult corneal endothelial cells in vivo lack proliferation in response to the cell loss caused by outer damages and diseases. As a result, in order to compensate for cell loss, corneal endothelial cells migrate and enlarge while not via dividing to increase the endothelial cell density. Therefore, it is not capable for corneal endothelium to restore the corneal clarity. Some researches have proved that in vitro the corneal endothelial maintained proliferation ability. This review describes the current research progress regarding the negative factors that inhibit proliferation of the corneal endothelial cells. This review will mainly present several genes and proteins that inhibit the proliferation of the corneal endothelial cells, of course including some other factors like enzymes and position. 展开更多
关键词 corneal endothelium PROLIFERATION age PLZF TELOMERASE PTP1B CAMP POSITION
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糖尿病性视网膜病变程度和糖化血红蛋白水平对糖尿病患者角膜内皮的影响
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作者 张越鑫 姜善好 《医学临床研究》 CAS 2024年第5期681-684,共4页
【目的】探讨糖尿病性视网膜病变(diabetic retinopathy,DR)程度和糖化血红蛋白(hemoglobin A1c,HbA1c)水平对糖尿病患者角膜内皮的影响。【方法】选取2023年2月至6月在烟台毓璜顶医院眼科诊治的167例(334眼)白内障患者,其中糖尿病患者... 【目的】探讨糖尿病性视网膜病变(diabetic retinopathy,DR)程度和糖化血红蛋白(hemoglobin A1c,HbA1c)水平对糖尿病患者角膜内皮的影响。【方法】选取2023年2月至6月在烟台毓璜顶医院眼科诊治的167例(334眼)白内障患者,其中糖尿病患者117例(234眼)纳入观察组,非糖尿病患者50例(100眼)纳入对照组。观察组按照DR的临床分期分为无DR(no diabetic retinopathy,NDR)组、DR非增殖期(nonproliferative diabetic retinopathy,NPDR)组和DR增殖期(proliferative diabetic retinopathy,PDR)组;根据入院后HbA1c结果分为A组(HbA1c<7.5%)、B组(7.5%≤HbA1c<8.5%)、C组(8.5%≤HbA1c<9.5%)和D组(HbA1c≥9.5%)。所有患者均采用内皮细胞显微镜检测角膜内皮细胞密度(endothelial corneal density,ECD)、细胞面积标准差(standard deviation,SD)、变异系数(variation coefficient,CV)、六角形细胞百分比(hexagonal cell percentage,HCR)、中央角膜厚度(central corneal thickness,CCT)并比较。【结果】与对照组相比,观察组ECD降低,SD、CV增加,差异有统计学意义(P<0.05),但两组HCR、CCT比较,差异无统计学意义(P>0.05)。与NDR组、NPDR组比较,PDR组ECD明显降低,差异均有统计学意义(P<0.05),但NDR组与NPDR组ECD比较,差异无统计学意义(P>0.05);NPDR组、PDR组CV与NDR组比较,差异均有统计学意义(P<0.05),SD与NDR组比较,差异无统计学意义(P>0.05),且NPDR组与PDR组SD、CV比较,差异均无统计学意义(P>0.05);NPDR组、PDR组、NDR组HCR、CCT比较,差异无统计学意义(P>0.05)。A组、B组、C组、D组ECD随着HbA1c水平升高而降低,且B组、C组、D组ECD、SD与A组比较,差异均有统计学意义(P<0.05);但B组、C组、D组ECD、SD比较,差异无统计学意义(P>0.05);A组和C组CV比较,差异有统计学意义(P<0.05)。A组CV与B组、D组比较,差异均无统计学意义(P>0.05);A组、B组、C组、D组HCR、CCT比较,差异均无统计学意义(P>0.05)。年龄与CCT呈负相关(P<0.05);糖尿病病程与ECD呈负相关(P<0.05),与SD、CV呈正相关性(P<0.05);HbA1c水平、DR严重程度与ECD呈负相关(P<0.05)。【结论】DR病变程度加重以及HbA1c水平升高均会导致ECD下降、CV升高,即随着糖尿病病程延长和血糖控制不稳定,角膜内皮细胞会发生数量和形态改变,并可导致角膜内皮细胞功能下降。 展开更多
关键词 糖尿病/并发症 糖尿病视网膜病变 糖基化血红蛋白A 内皮 角膜
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