目的探讨牙周内窥镜(periodontal endoscope,PE)辅助龈下刮治和根面平整术(scaling and root plan⁃ning,SRP)的临床疗效及对牙周炎患者心理和生活质量的影响,为临床应用牙周内窥镜提供参考。方法本研究已通过单位伦理委员会审查批准,并...目的探讨牙周内窥镜(periodontal endoscope,PE)辅助龈下刮治和根面平整术(scaling and root plan⁃ning,SRP)的临床疗效及对牙周炎患者心理和生活质量的影响,为临床应用牙周内窥镜提供参考。方法本研究已通过单位伦理委员会审查批准,并获得患者知情同意。收集2018年4月—2022年12月于南京大学医学院附属口腔医院牙周病科就诊的,传统SRP治疗6周后复查仍有残留牙周袋探诊深度(probing depth,PD)≥5 mm的牙周炎患者,进一步行PE辅助SRP(PE+SRP)。在传统SRP治疗后6周,PE+SRP治疗3个月后分别测量牙周临床指标包括菌斑指数(plaque index,PLI)、PD、临床附着丧失(clinical attachment loss,CAL)及探诊出血(bleeding on probing,BOP);同时分别收集传统SRP和PE辅助SRP治疗即刻视觉模拟评分量表(visual analogue scale,VAS);在传统SRP治疗和PE辅助SRP治疗3个月后分别收集牙周组织自我认知表、口腔健康影响程度量表⁃14(oral health impact profile⁃14,OHIP⁃14)以及牙科畏惧调查量表(dental fear scale,DFS)。结果共纳入牙周炎患者23例,患牙486颗,832个位点纳入临床研究。PE+SRP治疗后3个月,各项牙周临床指标:PLI(t=9.254,P<0.001)、PD(t=50.724,P<0.001)、CAL(t=22.407,P<0.001)以及BOP(t=9.217,P<0.001)均显著改善;与传统SRP(VAS:2.48±1.70)相比,PE+SRP(VAS:2.57±1.80)给患者带来的疼痛感无显著差异(t=0.192,P=0.850);两组牙周组织自我认知表得分无显著性差异(t=1.485,P=0.152);同时传统SRP完成后OHIP⁃14的得分为(12.13±7.63)分,PE+SRP完成后OHIP⁃14得分为(10.26±5.25)分,两者无显著差异(t=-1.589,P=0.126);传统SRP完成后DFS量表得分为(40.70±12.63)分,PE+SRP完成后DFS量表得分为(41.57±12.61)分,两者无显著差异(t=0.404,P=0.690)。结论PE辅助SRP治疗牙周炎患者残留牙周袋后各项牙周临床指标均显著改善,且与传统SRP相比,PE辅助SRP对牙周炎患者的生活质量和心理状况没有负面影响,可以临床广泛推广。展开更多
Adult stem cells (ASC) have been found in many tis-sues and are of great therapeutic potential due to their capability of differentiation. However, ASC comprise only a small fraction of the tissues. In order to use AS...Adult stem cells (ASC) have been found in many tis-sues and are of great therapeutic potential due to their capability of differentiation. However, ASC comprise only a small fraction of the tissues. In order to use ASC for therapeutic purposes, it is important to obtain relatively pure stem cells in large quantities. Current methods for stem cell purification are mainly based on marker-dependent cell sorting techniques, which have various technical difficulties. In this study, we have attempted to develop novel conditions to favor the growth of the dental follicle stem cells (DFSC) such that the resultant cell populations are enriched in stem cells. Specifically, a heterogeneous dental follicle cell (H-DFC) population containing stem cells and homogenous non-stem cell dental follicle cell population were cultured at 1% or 5% hypoxic conditions. Only the heterogeneous population could increase proliferation in the hypoxic condition whereas the homogenous DFC did not change their proliferation rate. In addition, when the resultant cells from the heterogonous population were subjected to differentiation, they appeared to have a higher capacity of adipogenesis and osteogenesis as compared to the controls grown in the normal at-mosphere (normoxic condition). These hypoxia- treated cells also express higher levels of some stem cell markers. Together, these data suggest that stem cells are enriched by culturing the heterogeneous cell populations in a reduced O2 condition.展开更多
Background: The autonomy of dental anxiety may be compared with other psychopathologies, however, it is classified as indicative of a specific phobia. It is interesting to know how dental anxiety operates within a wid...Background: The autonomy of dental anxiety may be compared with other psychopathologies, however, it is classified as indicative of a specific phobia. It is interesting to know how dental anxiety operates within a wider context. Material and Method: The group was made by 514 subjects, recruited from several dental surgeries. The entire process, consisted in self-compilation of the Dental Anxiety Scale (DAS), and the Patient Health Questionnaire (PHQ). The software used for statistical calculations was the Statistical Package for Social Sciences (SPSS) version 16.0. The chi-square test was used for the distribution of the nominal variables. Results: The analysis of data revealed a significantly different distribution between high and low levels of dental anxiety and comorbidity in relation to only one diagnostic category, namely mood disorders. Dental fears and comorbidity were mostly in line with our expectations, except about fear of needles with eating disorders. Dental anxiety is to be considered as a dimension on a par with other psychopathologies. Conclusions: The study of comorbidity between mood disorders and dental anxiety, suggests that dental anxiety rather than belonging to the family of anxiety disorders would be better classified along with mood disorders. The findings regarding emotional inducers (fears) in relation to the distribution of psychopathology are worth noting. The above observations reaffirm the need to form links between dentistry and psychiatry.展开更多
目的调查分析上海市部分成人牙科畏惧症(dental fear,DF)的流行情况及其构成因子。方法用DF调查量表(dental fear inventory,DFI)对来诊的成人患者进行主观问卷调查。结果牙科畏惧症检出率为86.15%,不同年龄、性别、文化程度的牙科患者D...目的调查分析上海市部分成人牙科畏惧症(dental fear,DF)的流行情况及其构成因子。方法用DF调查量表(dental fear inventory,DFI)对来诊的成人患者进行主观问卷调查。结果牙科畏惧症检出率为86.15%,不同年龄、性别、文化程度的牙科患者DF发生率存在差异,DF的五个构成因子中器械畏惧和卫生条件畏惧男女之间差别有统计学意义(P<0.05)。结论牙科畏惧症的发生由综合性因素造成,需要通过患者、医护人员的共同努力,将牙科畏惧症降低到最低限度。展开更多
文摘目的探讨牙周内窥镜(periodontal endoscope,PE)辅助龈下刮治和根面平整术(scaling and root plan⁃ning,SRP)的临床疗效及对牙周炎患者心理和生活质量的影响,为临床应用牙周内窥镜提供参考。方法本研究已通过单位伦理委员会审查批准,并获得患者知情同意。收集2018年4月—2022年12月于南京大学医学院附属口腔医院牙周病科就诊的,传统SRP治疗6周后复查仍有残留牙周袋探诊深度(probing depth,PD)≥5 mm的牙周炎患者,进一步行PE辅助SRP(PE+SRP)。在传统SRP治疗后6周,PE+SRP治疗3个月后分别测量牙周临床指标包括菌斑指数(plaque index,PLI)、PD、临床附着丧失(clinical attachment loss,CAL)及探诊出血(bleeding on probing,BOP);同时分别收集传统SRP和PE辅助SRP治疗即刻视觉模拟评分量表(visual analogue scale,VAS);在传统SRP治疗和PE辅助SRP治疗3个月后分别收集牙周组织自我认知表、口腔健康影响程度量表⁃14(oral health impact profile⁃14,OHIP⁃14)以及牙科畏惧调查量表(dental fear scale,DFS)。结果共纳入牙周炎患者23例,患牙486颗,832个位点纳入临床研究。PE+SRP治疗后3个月,各项牙周临床指标:PLI(t=9.254,P<0.001)、PD(t=50.724,P<0.001)、CAL(t=22.407,P<0.001)以及BOP(t=9.217,P<0.001)均显著改善;与传统SRP(VAS:2.48±1.70)相比,PE+SRP(VAS:2.57±1.80)给患者带来的疼痛感无显著差异(t=0.192,P=0.850);两组牙周组织自我认知表得分无显著性差异(t=1.485,P=0.152);同时传统SRP完成后OHIP⁃14的得分为(12.13±7.63)分,PE+SRP完成后OHIP⁃14得分为(10.26±5.25)分,两者无显著差异(t=-1.589,P=0.126);传统SRP完成后DFS量表得分为(40.70±12.63)分,PE+SRP完成后DFS量表得分为(41.57±12.61)分,两者无显著差异(t=0.404,P=0.690)。结论PE辅助SRP治疗牙周炎患者残留牙周袋后各项牙周临床指标均显著改善,且与传统SRP相比,PE辅助SRP对牙周炎患者的生活质量和心理状况没有负面影响,可以临床广泛推广。
文摘Adult stem cells (ASC) have been found in many tis-sues and are of great therapeutic potential due to their capability of differentiation. However, ASC comprise only a small fraction of the tissues. In order to use ASC for therapeutic purposes, it is important to obtain relatively pure stem cells in large quantities. Current methods for stem cell purification are mainly based on marker-dependent cell sorting techniques, which have various technical difficulties. In this study, we have attempted to develop novel conditions to favor the growth of the dental follicle stem cells (DFSC) such that the resultant cell populations are enriched in stem cells. Specifically, a heterogeneous dental follicle cell (H-DFC) population containing stem cells and homogenous non-stem cell dental follicle cell population were cultured at 1% or 5% hypoxic conditions. Only the heterogeneous population could increase proliferation in the hypoxic condition whereas the homogenous DFC did not change their proliferation rate. In addition, when the resultant cells from the heterogonous population were subjected to differentiation, they appeared to have a higher capacity of adipogenesis and osteogenesis as compared to the controls grown in the normal at-mosphere (normoxic condition). These hypoxia- treated cells also express higher levels of some stem cell markers. Together, these data suggest that stem cells are enriched by culturing the heterogeneous cell populations in a reduced O2 condition.
文摘Background: The autonomy of dental anxiety may be compared with other psychopathologies, however, it is classified as indicative of a specific phobia. It is interesting to know how dental anxiety operates within a wider context. Material and Method: The group was made by 514 subjects, recruited from several dental surgeries. The entire process, consisted in self-compilation of the Dental Anxiety Scale (DAS), and the Patient Health Questionnaire (PHQ). The software used for statistical calculations was the Statistical Package for Social Sciences (SPSS) version 16.0. The chi-square test was used for the distribution of the nominal variables. Results: The analysis of data revealed a significantly different distribution between high and low levels of dental anxiety and comorbidity in relation to only one diagnostic category, namely mood disorders. Dental fears and comorbidity were mostly in line with our expectations, except about fear of needles with eating disorders. Dental anxiety is to be considered as a dimension on a par with other psychopathologies. Conclusions: The study of comorbidity between mood disorders and dental anxiety, suggests that dental anxiety rather than belonging to the family of anxiety disorders would be better classified along with mood disorders. The findings regarding emotional inducers (fears) in relation to the distribution of psychopathology are worth noting. The above observations reaffirm the need to form links between dentistry and psychiatry.
文摘目的调查分析上海市部分成人牙科畏惧症(dental fear,DF)的流行情况及其构成因子。方法用DF调查量表(dental fear inventory,DFI)对来诊的成人患者进行主观问卷调查。结果牙科畏惧症检出率为86.15%,不同年龄、性别、文化程度的牙科患者DF发生率存在差异,DF的五个构成因子中器械畏惧和卫生条件畏惧男女之间差别有统计学意义(P<0.05)。结论牙科畏惧症的发生由综合性因素造成,需要通过患者、医护人员的共同努力,将牙科畏惧症降低到最低限度。
