The morpohologic feature of radicular dentin in sensitive and naturally desensitive area was observed with scanning electron microscopy (SEM). Eight specimens were taken from six patients with periodontal diseases. It...The morpohologic feature of radicular dentin in sensitive and naturally desensitive area was observed with scanning electron microscopy (SEM). Eight specimens were taken from six patients with periodontal diseases. It was established that the orifices of dentinal tubules in hypersensitive areas were much more than those in naturally desensitive areas. The dimension of the orifices in hyperoensitive areas was much larger than that in naturally desensitive areas. The results suggest that theie is relationship between dentin moophology and dentin hypersensitivity. In this article, a new dentin bipopsy technique with hollow drill was reported also.展开更多
The morphologic feature of radicular dentin in sensitive and naturally desensitive areas was observed with scanning electron microscopy(SEM). Eight specimens were taken from six-patients with periodontal diseases. It ...The morphologic feature of radicular dentin in sensitive and naturally desensitive areas was observed with scanning electron microscopy(SEM). Eight specimens were taken from six-patients with periodontal diseases. It was established that the orifices of dentinal tubules in hypersensitive areas were much more than those in naturally desensitive areas. The dimension of the orifices in hypersensitive areas was much larger than that in naturally desensitive areas. The results suggest that there is relationship between dentin morphology and dentin hypersensitivity. In this article, a new technique of dentin biopsy with hollow drill was reported also.展开更多
Dentine hypersensitivity is an annoying worldwide disease,yet its mechanism remains unclear.The long-used hydrodynamic theory,a stimuli-induced fluid-flow process,describes the pain processes.However,no experimental e...Dentine hypersensitivity is an annoying worldwide disease,yet its mechanism remains unclear.The long-used hydrodynamic theory,a stimuli-induced fluid-flow process,describes the pain processes.However,no experimental evidence supports the statements.Here,we demonstrate that stimuli-induced directional cation transport,rather than fluid-flow,through dentinal tubules actually leads to dentine hypersensitivity.The in vitro/in vivo electro-chemical and electro-neurophysiological approaches reveal the cation current through the nanoconfined negatively charged dentinal tubules coming from external stimuli(pressure,pH,and temperature)on dentin surface and further triggering the nerve impulses causing the dentine hypersensitivity.Furthermore,the cationic-hydrogels blocked dentinal tubules could significantly reduce the stimuli-triggered nerve action potentials and the anionhydrogels counterpart enhances those,supporting the cation-flow transducing dentine hypersensitivity.Therefore,the inspired ion-blocking desensitizing therapies have achieved remarkable pain relief in clinical applications.The proposed mechanism would enrich the basic knowledge of dentistry and further foster breakthrough initiatives in hypersensitivity mitigation and cure.展开更多
Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and des...Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and desensitizing toothpaste are standard clinical treatment strategies,but unsatisfactory treatment modalities are marked by long-term administration,poor dentinal tubule closure,microleakage,and the development of secondary caries.To improve the treatment efficiency of DH,numerous organic or inorganic biomaterials have been developed to relieve toothache and reverse the instability of desensitization.Biomaterials are expected to participate in dental remineralization to achieve desensitization.This review discusses various biomaterials for DH therapy based on different desensitization mechanisms,including dentinal tubule closure and dental nerve blockade,and presents a perspective on the underlying future of dentin regeneration medicine for DH therapy.展开更多
The object of this study was to evaluate the effect of bioactive glass （BG） size on mineral formation on dentin surfaces. Totally demineralized dentin discs were treated using BG suspensions with different particle ...The object of this study was to evaluate the effect of bioactive glass （BG） size on mineral formation on dentin surfaces. Totally demineralized dentin discs were treated using BG suspensions with different particle sizes： i.e., microscale bioactive glass （m-BG）, submicroscale bioactive glass （sm-BG） and nanoscale bioactive glass （n-BG）. Field-emission scanning electron microscopy and 3D profile measurement laser microscopy were used to observe the surface morphology and roughness. It was found that all BG particles could promoted mineral formation on dentin surfaces, while plug-like depositions were observed on the dentin discs treated by n-BG and they were more acid-resistant. The present results may imply that n-BG has potential clinical application for dentin hypersensitivity treatment.展开更多
Dental hard tissues,primarily enamel and dentin,serving essential functions such as cutting,chewing,speaking,and maintaining facial aesthetics,mainly composed well-aligned hydroxyapatite(HAp)nanocrystals interlaced wi...Dental hard tissues,primarily enamel and dentin,serving essential functions such as cutting,chewing,speaking,and maintaining facial aesthetics,mainly composed well-aligned hydroxyapatite(HAp)nanocrystals interlaced with a protein matrix.These tissues exhibit remarkable mechanical and aesthetic behaviors.However,once damaged,its ability to self-repair is extremely limited,often accompanied by dentin hypersensitivity(DH).Currently,although dental restorations using synthetic materials and remineralization techniques have made clinical progress,these methods still have limitations that affect their widespread use in clinical applications.Therefore,understanding the formation mechanisms of dental hard tissues and developing high-performance restorative technologies that can mimic natural teeth and meet clinical needs are crucial.This review focuses on the current strategies and research advancements in enamel regeneration and dentin desensitization,and challenges of clinical translation.We emphasize that scientific research should start with clinical needs,and these studies,through translation,ultimately serve the clinic to form a mutually reinforcing virtuous cycle.This review aims to provide a new perspective on the prevention and treatment of dental hard tissues,promote innovation in restorative materials and techniques,and bring better clinical translation products and services to patients.展开更多
文摘The morpohologic feature of radicular dentin in sensitive and naturally desensitive area was observed with scanning electron microscopy (SEM). Eight specimens were taken from six patients with periodontal diseases. It was established that the orifices of dentinal tubules in hypersensitive areas were much more than those in naturally desensitive areas. The dimension of the orifices in hyperoensitive areas was much larger than that in naturally desensitive areas. The results suggest that theie is relationship between dentin moophology and dentin hypersensitivity. In this article, a new dentin bipopsy technique with hollow drill was reported also.
文摘The morphologic feature of radicular dentin in sensitive and naturally desensitive areas was observed with scanning electron microscopy(SEM). Eight specimens were taken from six-patients with periodontal diseases. It was established that the orifices of dentinal tubules in hypersensitive areas were much more than those in naturally desensitive areas. The dimension of the orifices in hypersensitive areas was much larger than that in naturally desensitive areas. The results suggest that there is relationship between dentin morphology and dentin hypersensitivity. In this article, a new technique of dentin biopsy with hollow drill was reported also.
基金We thank the National Key R&D Program of China(No.2020YFA0710401)the National Natural Science Foundation of China(Nos.82225012,81922019,82071161,81991505,22122207,21988102,and 52075138)+1 种基金the Young Elite Scientist Sponsorship Program by CAST(No.2020QNRC001)the Beijing Nova Program(No.211100002121013).
文摘Dentine hypersensitivity is an annoying worldwide disease,yet its mechanism remains unclear.The long-used hydrodynamic theory,a stimuli-induced fluid-flow process,describes the pain processes.However,no experimental evidence supports the statements.Here,we demonstrate that stimuli-induced directional cation transport,rather than fluid-flow,through dentinal tubules actually leads to dentine hypersensitivity.The in vitro/in vivo electro-chemical and electro-neurophysiological approaches reveal the cation current through the nanoconfined negatively charged dentinal tubules coming from external stimuli(pressure,pH,and temperature)on dentin surface and further triggering the nerve impulses causing the dentine hypersensitivity.Furthermore,the cationic-hydrogels blocked dentinal tubules could significantly reduce the stimuli-triggered nerve action potentials and the anionhydrogels counterpart enhances those,supporting the cation-flow transducing dentine hypersensitivity.Therefore,the inspired ion-blocking desensitizing therapies have achieved remarkable pain relief in clinical applications.The proposed mechanism would enrich the basic knowledge of dentistry and further foster breakthrough initiatives in hypersensitivity mitigation and cure.
基金This work was financially supported by the Special Program for Medical and Health Professionals of Jilin Province(No.JLSWSRCZX2021-085)the Achievement Transformation Fund of the First Hospital of Jilin University(Nos.JDYYZH-2102055 and JDYYZH-2102013).
文摘Dentin hypersensitivity(DH)associated with dentinal tubule exposure is one of the most common causes of toothache with a rapid onset and short duration.Medication,filling repair,laser irradiation,crown therapy,and desensitizing toothpaste are standard clinical treatment strategies,but unsatisfactory treatment modalities are marked by long-term administration,poor dentinal tubule closure,microleakage,and the development of secondary caries.To improve the treatment efficiency of DH,numerous organic or inorganic biomaterials have been developed to relieve toothache and reverse the instability of desensitization.Biomaterials are expected to participate in dental remineralization to achieve desensitization.This review discusses various biomaterials for DH therapy based on different desensitization mechanisms,including dentinal tubule closure and dental nerve blockade,and presents a perspective on the underlying future of dentin regeneration medicine for DH therapy.
基金the financial support from the National Natural Science Foundation of China (No. 51372005)
文摘The object of this study was to evaluate the effect of bioactive glass （BG） size on mineral formation on dentin surfaces. Totally demineralized dentin discs were treated using BG suspensions with different particle sizes： i.e., microscale bioactive glass （m-BG）, submicroscale bioactive glass （sm-BG） and nanoscale bioactive glass （n-BG）. Field-emission scanning electron microscopy and 3D profile measurement laser microscopy were used to observe the surface morphology and roughness. It was found that all BG particles could promoted mineral formation on dentin surfaces, while plug-like depositions were observed on the dentin discs treated by n-BG and they were more acid-resistant. The present results may imply that n-BG has potential clinical application for dentin hypersensitivity treatment.
基金the funding from the National Science Fund for Distinguished Young Scholars(52225301)the National Key Research and Development Program of China(2020YFA0710400 and 2020YFA0710402)+4 种基金the 111 Project(B14041),the International Science and Technology Cooperation Program of Shaanxi Province(2022KWZ-24)the Fundamental Research Funds for the Central Universities(GK202305001 and GK202309034)the Key Science&Technology Innovation Team of Shaanxi Province(2022TD-35)the funding from the National Key Research and Development Program of China(2022YFC2405900 and 2022YFC2405902)the funding from the National Natural Science Foundation of China(82370949)。
文摘Dental hard tissues,primarily enamel and dentin,serving essential functions such as cutting,chewing,speaking,and maintaining facial aesthetics,mainly composed well-aligned hydroxyapatite(HAp)nanocrystals interlaced with a protein matrix.These tissues exhibit remarkable mechanical and aesthetic behaviors.However,once damaged,its ability to self-repair is extremely limited,often accompanied by dentin hypersensitivity(DH).Currently,although dental restorations using synthetic materials and remineralization techniques have made clinical progress,these methods still have limitations that affect their widespread use in clinical applications.Therefore,understanding the formation mechanisms of dental hard tissues and developing high-performance restorative technologies that can mimic natural teeth and meet clinical needs are crucial.This review focuses on the current strategies and research advancements in enamel regeneration and dentin desensitization,and challenges of clinical translation.We emphasize that scientific research should start with clinical needs,and these studies,through translation,ultimately serve the clinic to form a mutually reinforcing virtuous cycle.This review aims to provide a new perspective on the prevention and treatment of dental hard tissues,promote innovation in restorative materials and techniques,and bring better clinical translation products and services to patients.
