As a new type of environmental pollutants,microplastics have gradually attracted people's attention.A large number of plastics discharged into the environment by human beings are constantly aging and breaking,and ...As a new type of environmental pollutants,microplastics have gradually attracted people's attention.A large number of plastics discharged into the environment by human beings are constantly aging and breaking,and finally become microplastics.Microplastics can adsorb pollutants in the environment,and their components have certain toxicity,which can cause different degrees of harm to organisms.Due to the structural characteristics of microplastic particles,such as small particle size,large specific surface area,and their distribution in different environmental media,it is very difficult to accurately detect microplastics.Reliable collection and detection methods are the key to the study of environmental behavior of microplastics.In this study,the collection and detection methods of microplastics in the environment were reviewed,and the development direction of microplastics detection technology in the future was prospected.This study has a certain reference value for the related research and the prevention and treatment of micro-plastic pollution.展开更多
Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction o...Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction of bridge pile foundations,accidents such as hole collapse,slurry leakage,and drill sticking will easily occur.In this paper,the principle and method of sonar detection for detecting karst caves at the bottom of bridge piles was introduced,and the sonar detection data and the cave situation at the bottom of the pile during the construction process in combination with the case of Yunnan Zhenguo Highway Project was analyzed,which verifies the practicability and reliability of sonar detection method reliability.展开更多
Surface irradiance measurements with high temporal resolution can be used to detect clear skies,which is a critical step for further study,such as aerosol and cloud radiative effects.Twenty-one clear-sky detection(CSD...Surface irradiance measurements with high temporal resolution can be used to detect clear skies,which is a critical step for further study,such as aerosol and cloud radiative effects.Twenty-one clear-sky detection(CSD)methods are assessed based on five years of 1-min surface irradiance data at Xianghe—a heavily polluted station on the North China Plain.Total-sky imager(TSI)discrimination results corrected by manual checks are used as the benchmark for the evaluation.The performance heavily relies on the criteria adopted by the CSD methods.Those with higher cloudy-sky detection accuracy rates produce lower clear-sky accuracy rates,and vice versa.A general tendency in common among all CSD methods is the detection accuracy deteriorates when aerosol loading increases.Nearly all criteria adopted in CSD methods are too strict to detect clear skies under polluted conditions,which is more severe if clear-sky irradiance is not properly estimated.The mean true positive rate(CSD method correctly detects clear sky)decreases from 45%for aerosol optical depth(AOD)≤0.2%to 6%for AOD>0.5.The results clearly indicate that CSD methods in a highly polluted region still need further improvements.展开更多
SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative proba...SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative probabilities between the number of anticyclonic/cyclonic ME fronts(AEF/CEF)and the number of anticyclones/cyclones reached 20%.The northeastern and southwestern parts of these anticyclones had more fronts than the northwestern and southeastern parts,although CEFs were nearly equally distributed in all directions.The number of ME fronts had remarkable seasonal variations,while the eddy kinetic energy(EKE)showed no seasonal variations.The total EKE at the ME fronts was three times of that within the MEs,and it was much stronger in AEFs than in CEFs.The interannual variability in the number of ME fronts and EKE had no significant correlation with the El Ni?o-Southern Oscillation(ENSO)index.Possible mechanisms of ME fronts were discussed,but the contributions of mesoscale eddies to SST fronts need to be quantified in future studies.展开更多
In this study, fructose and glucose contents in honey were determined by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method. According to the results,...In this study, fructose and glucose contents in honey were determined by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method. According to the results, there were great differences between determination results of reducing sugar contents by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method. Specifically, average determination results of reducing sugar contents by liquid chromatography-refractive index detection method were reduced by about 9.5% compared with alkaline copper tartrate solution-direct titra- tion method. Subsequently, determination results of reducing sugar contents by two methods were compared and analyzed. Liquid chromatography-refractive index detection method was used to determine fructose and glucose monomers in honey, but alkaline copper tartrate solution-direct titration method was used to determine reducing sugar composition in honey, which might lead to significantly different results. Due to small sample size and limited conditions, the determination results were not necessarily representative. Bee product acquisition and processing enterprises and relevant departments should pay much attention on these issues and fully consider the current situation of grass-roots units to develop scientific, rigorous, simple, universal, convenient, low-cost and practicable standards, thus ensuring the safety and reliability of food quality.展开更多
A reliable method is described for the detection of amitoride,triamterene. canrenone and spironolactone in human urine using GC-MS analysis after trimethytsitytation.The mass spectrometric and metabotic features of th...A reliable method is described for the detection of amitoride,triamterene. canrenone and spironolactone in human urine using GC-MS analysis after trimethytsitytation.The mass spectrometric and metabotic features of the compounds are discussed展开更多
To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entr...To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entropy of content name of interest packet.On the one hand,taking advantage of the characteristics of self-similarity is very sensitive to traffic changes,calculating the Hurst index of the traffic,to identify initial IFA attacks.On the other hand,according to the randomness of user requests,calculating the information entropy of content name of the interest packets,to detect the severity of the IFA attack,is.Finally,based on the above two aspects,we use the bilateral detection method based on non-parametric CUSUM algorithm to judge the possible attack behavior in CCN.The experimental results show that flooding attack detection method proposed for CCN can not only detect the attack behavior at the early stage of attack in CCN,but also is more accurate and effective than other methods.展开更多
[Objective] More accurate, rapid and sensitive method of melamine and cyanuricacid residue in dairy products and feedstuff were re- viewed. [ Method] Physicochemical properties, metabolism, uses, harm and detection me...[Objective] More accurate, rapid and sensitive method of melamine and cyanuricacid residue in dairy products and feedstuff were re- viewed. [ Method] Physicochemical properties, metabolism, uses, harm and detection methods of melamine and cyanuric acid were analyzed and described. [ Result] Melamine and cyanuric acid, when used alone, were slightly toxic, but long -term intake could lead to animal reproductive and urinary system damage. [ Condusion] Establishing a more sensitive, fast and easy to popularize detection method for elarnine and cyanuricacid res- idue in dairy products and feedstuff was necessary.展开更多
Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems a...Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems are essentially required due to lack of effective vaccine against dengue virus and the progressive spread of the dengue virus infection.The recent progress in developing micro-and nano-fabrication techniques has led to low cost and scale down the biomedical point-of-care devices.Starting from the conventional and modern available methods for the diagnosis of dengue infection,this review examines several emerging rapid and point-of-care diagnostic devices that hold significant potential for the progress in smart diagnosis tools.The given review revealed that an effective vaccine is required urgently against all the dengue virus serotypes.However,the rapid detection methods of dengue virus help in early treatment and significantly reduce the dengue virus outbreak.展开更多
Bactrocera dorsalis Hendel （Diptera： Tephritidae） is an invasive pest around the world. The paper summarizes biological and ecological characteristics of B, dorsalis, and reviews its detection methods from the aspe...Bactrocera dorsalis Hendel （Diptera： Tephritidae） is an invasive pest around the world. The paper summarizes biological and ecological characteristics of B, dorsalis, and reviews its detection methods from the aspects of morphological identification, acoustic detection and molecular detection, in order to provide a reference for further research and development of new detection methods. The hot issues in the study of B. dorsalis, such as ecological adaptation pattern, diffusion pathways and mechanisms, sustainable control measures, are also put forward in the paper.展开更多
Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinica...Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.展开更多
The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomati...The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomatic infections is increasing as COVID-19 research continues and SARS-CoV-2 testing improves.Many asymptomatic COVID-19 patients with no obvious clinical symptoms are able to transmit the virus to others due to the presence of SARS-CoV-2 in their bodies,thus greatly accelerating the worldwide pandemic and posing great difficulty and threat to global epidemic prevention and control.Therefore,early detection and identification of asymptomatic infected persons through various testing methods are effective measures to control the COVID-19 pandemic.This article will provide a brief review of the available methods and techniques for detecting asymptomatic COVID-19 patients.展开更多
Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently, virus isolation and identification is still the traditional method for the detection...Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently, virus isolation and identification is still the traditional method for the detection of Koi herpes virus, while molecular biology detection method has become the current developmental di- rection due to its characteristics of more sensitive, specific and rapid. Furthermore, people are still committed to exploring new detection methods for the detection of Koi herpes vires. In this paper, traditional and newly-developed detection methods of Koi herpes vires in recent years were summarized, in order to provide refer- ence for further exploring rapid and accurate diagnostic detection method.展开更多
Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great ...Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great harm to the breeding industry.In this paper,the harm of mycotoxin to animals and its detection methods in feed and animals are summarized and analyzed,so as to provide the reference basis for further improving the detection methods of mycotoxin and reducing the harm of mycotoxin to breeding industry.展开更多
In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators hav...In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.展开更多
This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. ...This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. Authors previously investigated the physical characteristics of hot-spot phenomenon referring to internal crystal defect. Based on it, a hot-spot detection method named as current-based SRC （self reverse current） detection method is developed. However, it becomes extraordinarily complicated to determine the defective cells under low illumination. In order to avoid this disadvantage, authors improve the SRC detection method by applying voltage. From the feasibility experiment results, it is confirmed that by calculating cell HSI （hotspots index） with voltage, the PV modules with defective cells can be prospectively excluded even under low illumination.展开更多
Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foo... Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.……展开更多
Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell the...Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell therapy,a promising branch of adoptive T-cell therapy,has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer.At present,CAR-T cells are predominantly used to treat hematological malignancies,although their application in solid tumors is being readily investigated.Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy,few have evaluated predictors of CAR-T cell therapeutic efficacy.Thus,the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy,with a particular focus on biomarkers and their detection methods.展开更多
A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encod...A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encode the coat protein of WSMV, 2 pairs of primers were designed. Final y, the 1st pair of primers was select-ed through the specificity test. The sensitivity test showed the sensitivity of RT-LAMP method was 10 times higher than that of RT-PCR. In addition, the amplifica-tion of target gene could be judged visual y from the presence of fluorescence （cal-cein） in the final reaction system. The RT-LAMP method, established in this study, was rapid, easy, specific and sensitive. Moreover, it did not require sophisticated equip-ment. The RT-LAMP was suitable for the rapid detection of WSMV.展开更多
基金Supported by Project of National Center of Technology Innovation for Dairy"Study on the Key Technologies of Microplastics Detection for New Pollutants in Dairy Ingredient Water"(2023-KFKT-24).
文摘As a new type of environmental pollutants,microplastics have gradually attracted people's attention.A large number of plastics discharged into the environment by human beings are constantly aging and breaking,and finally become microplastics.Microplastics can adsorb pollutants in the environment,and their components have certain toxicity,which can cause different degrees of harm to organisms.Due to the structural characteristics of microplastic particles,such as small particle size,large specific surface area,and their distribution in different environmental media,it is very difficult to accurately detect microplastics.Reliable collection and detection methods are the key to the study of environmental behavior of microplastics.In this study,the collection and detection methods of microplastics in the environment were reviewed,and the development direction of microplastics detection technology in the future was prospected.This study has a certain reference value for the related research and the prevention and treatment of micro-plastic pollution.
文摘Karst landforms are widely distributed in China,and are most common in Yunnan,Guizhou and Guangxi.If the development of karst caves at the bottom of the piles cannot be accurately ascertained before the construction of bridge pile foundations,accidents such as hole collapse,slurry leakage,and drill sticking will easily occur.In this paper,the principle and method of sonar detection for detecting karst caves at the bottom of bridge piles was introduced,and the sonar detection data and the cave situation at the bottom of the pile during the construction process in combination with the case of Yunnan Zhenguo Highway Project was analyzed,which verifies the practicability and reliability of sonar detection method reliability.
基金supported by the National Key R&D Program of China grant number 2017YFA0603504the Strategic Priority Research Program of the Chinese Academy of Sciences grant number XDA17010101the National Natural Science Foundation of Chinagrant number 41875183。
文摘Surface irradiance measurements with high temporal resolution can be used to detect clear skies,which is a critical step for further study,such as aerosol and cloud radiative effects.Twenty-one clear-sky detection(CSD)methods are assessed based on five years of 1-min surface irradiance data at Xianghe—a heavily polluted station on the North China Plain.Total-sky imager(TSI)discrimination results corrected by manual checks are used as the benchmark for the evaluation.The performance heavily relies on the criteria adopted by the CSD methods.Those with higher cloudy-sky detection accuracy rates produce lower clear-sky accuracy rates,and vice versa.A general tendency in common among all CSD methods is the detection accuracy deteriorates when aerosol loading increases.Nearly all criteria adopted in CSD methods are too strict to detect clear skies under polluted conditions,which is more severe if clear-sky irradiance is not properly estimated.The mean true positive rate(CSD method correctly detects clear sky)decreases from 45%for aerosol optical depth(AOD)≤0.2%to 6%for AOD>0.5.The results clearly indicate that CSD methods in a highly polluted region still need further improvements.
基金The National Natural Science Foundation of China under contract No.41976002。
文摘SST fronts at the mesoscale eddy edge(ME fronts)were investigated from 2007–2017 in the northern South China Sea(NSCS)based on an automatic method using satellite sea level anomaly(SLA)and SST data.The relative probabilities between the number of anticyclonic/cyclonic ME fronts(AEF/CEF)and the number of anticyclones/cyclones reached 20%.The northeastern and southwestern parts of these anticyclones had more fronts than the northwestern and southeastern parts,although CEFs were nearly equally distributed in all directions.The number of ME fronts had remarkable seasonal variations,while the eddy kinetic energy(EKE)showed no seasonal variations.The total EKE at the ME fronts was three times of that within the MEs,and it was much stronger in AEFs than in CEFs.The interannual variability in the number of ME fronts and EKE had no significant correlation with the El Ni?o-Southern Oscillation(ENSO)index.Possible mechanisms of ME fronts were discussed,but the contributions of mesoscale eddies to SST fronts need to be quantified in future studies.
文摘In this study, fructose and glucose contents in honey were determined by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method. According to the results, there were great differences between determination results of reducing sugar contents by liquid chromatography-refractive index detection method and alkaline copper tartrate solution-direct titration method. Specifically, average determination results of reducing sugar contents by liquid chromatography-refractive index detection method were reduced by about 9.5% compared with alkaline copper tartrate solution-direct titra- tion method. Subsequently, determination results of reducing sugar contents by two methods were compared and analyzed. Liquid chromatography-refractive index detection method was used to determine fructose and glucose monomers in honey, but alkaline copper tartrate solution-direct titration method was used to determine reducing sugar composition in honey, which might lead to significantly different results. Due to small sample size and limited conditions, the determination results were not necessarily representative. Bee product acquisition and processing enterprises and relevant departments should pay much attention on these issues and fully consider the current situation of grass-roots units to develop scientific, rigorous, simple, universal, convenient, low-cost and practicable standards, thus ensuring the safety and reliability of food quality.
文摘A reliable method is described for the detection of amitoride,triamterene. canrenone and spironolactone in human urine using GC-MS analysis after trimethytsitytation.The mass spectrometric and metabotic features of the compounds are discussed
基金This work was supported by the National Natural Science Foundation of China No.61672101the Beijing Key Laboratory of Internet Culture and Digital Dissemination Research(ICDDXN004)Key Lab of Information Network Security,Ministry of Public Security,No.C18601.
文摘To improve the attack detection capability of content centric network(CCN),we propose a detection method of interest flooding attack(IFA)making use of the feature of self-similarity of traffic and the information entropy of content name of interest packet.On the one hand,taking advantage of the characteristics of self-similarity is very sensitive to traffic changes,calculating the Hurst index of the traffic,to identify initial IFA attacks.On the other hand,according to the randomness of user requests,calculating the information entropy of content name of the interest packets,to detect the severity of the IFA attack,is.Finally,based on the above two aspects,we use the bilateral detection method based on non-parametric CUSUM algorithm to judge the possible attack behavior in CCN.The experimental results show that flooding attack detection method proposed for CCN can not only detect the attack behavior at the early stage of attack in CCN,but also is more accurate and effective than other methods.
文摘[Objective] More accurate, rapid and sensitive method of melamine and cyanuricacid residue in dairy products and feedstuff were re- viewed. [ Method] Physicochemical properties, metabolism, uses, harm and detection methods of melamine and cyanuric acid were analyzed and described. [ Result] Melamine and cyanuric acid, when used alone, were slightly toxic, but long -term intake could lead to animal reproductive and urinary system damage. [ Condusion] Establishing a more sensitive, fast and easy to popularize detection method for elarnine and cyanuricacid res- idue in dairy products and feedstuff was necessary.
基金supported by the Scientific Research Fund of the Shenzhen International cooperation projects under Grant Nos.(GJHZ20190819151403615)the Natural Science Youth Foundation of China(61801307).
文摘Dengue virus infections are increasing worldwide generally and in Asia,Central and South America and Africa,particularly.It poses a serious threat to the children population.The rapid and accurate diagnostic systems are essentially required due to lack of effective vaccine against dengue virus and the progressive spread of the dengue virus infection.The recent progress in developing micro-and nano-fabrication techniques has led to low cost and scale down the biomedical point-of-care devices.Starting from the conventional and modern available methods for the diagnosis of dengue infection,this review examines several emerging rapid and point-of-care diagnostic devices that hold significant potential for the progress in smart diagnosis tools.The given review revealed that an effective vaccine is required urgently against all the dengue virus serotypes.However,the rapid detection methods of dengue virus help in early treatment and significantly reduce the dengue virus outbreak.
基金Supported by International Cooperation Project of the Ministry of Science and Technology(2011DFB30040)Key Project of Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(2012JZ08)Scientific and Technological Projects of Nanning Municipal Science and Technology Bureau(20132308)
文摘Bactrocera dorsalis Hendel （Diptera： Tephritidae） is an invasive pest around the world. The paper summarizes biological and ecological characteristics of B, dorsalis, and reviews its detection methods from the aspects of morphological identification, acoustic detection and molecular detection, in order to provide a reference for further research and development of new detection methods. The hot issues in the study of B. dorsalis, such as ecological adaptation pattern, diffusion pathways and mechanisms, sustainable control measures, are also put forward in the paper.
基金Supported by grants from the Natural Science Foundation of Heilongjiang Province(No.H201335)Development Center for Medical Science and Technology,Ministry of Health,China(No.W2012FZ105)
文摘Circulating tumor cells(CTCs) are tumor cells that enter the blood circulation after detaching from the primary tumor and can migrate to reach distant organs, where they can give rise to aggressive metastasis. Clinical studies have revealed that the presence of CTCs in peripheral blood is correlated with disease progression in lung cancer. However, as CTCs are rare cancer cells released from tumors into the bloodstream, both enrichment and sensitive detection methods are technically challenging. In order to best understand how CTCs are currently being deployed, this review mainly focuses on the different detection methods for CTCs. Furthermore, we will describe the clinical impact of circulating tumor cells in lung cancer and discuss their potential use as biomarker to guide the prognosis.
基金The 940 Hospital COVID-19 Emergency Medical Research Proj ect(No.20yjky020)Scientific Research Foundation of Huoshenshan Hospital(No.HSS-217)。
文摘The global pandemic of COVID-19 has brought great harm and challenges to many countries and regions around the world.The majority of COVID-19 patients have typical clinical manifestations,but the number of asymptomatic infections is increasing as COVID-19 research continues and SARS-CoV-2 testing improves.Many asymptomatic COVID-19 patients with no obvious clinical symptoms are able to transmit the virus to others due to the presence of SARS-CoV-2 in their bodies,thus greatly accelerating the worldwide pandemic and posing great difficulty and threat to global epidemic prevention and control.Therefore,early detection and identification of asymptomatic infected persons through various testing methods are effective measures to control the COVID-19 pandemic.This article will provide a brief review of the available methods and techniques for detecting asymptomatic COVID-19 patients.
基金Supported by Project of Jilin Provincial Science and Technology Commission(20080218)
文摘Koi herpes virus is a new virus found in the aquaculture production of Cryprinus carpiod and common carp in recent years. Currently, virus isolation and identification is still the traditional method for the detection of Koi herpes virus, while molecular biology detection method has become the current developmental di- rection due to its characteristics of more sensitive, specific and rapid. Furthermore, people are still committed to exploring new detection methods for the detection of Koi herpes vires. In this paper, traditional and newly-developed detection methods of Koi herpes vires in recent years were summarized, in order to provide refer- ence for further exploring rapid and accurate diagnostic detection method.
基金Major Science and Technology Innovation Project of Shandong Province(2019JZZy 020609-03).
文摘Mycotoxin is widely distributed in various feedstuffs and feeds of animals.Mycotoxin not only causes feed deterioration,but also leads to various diseases of animals,or even results in acute toxic death,causing great harm to the breeding industry.In this paper,the harm of mycotoxin to animals and its detection methods in feed and animals are summarized and analyzed,so as to provide the reference basis for further improving the detection methods of mycotoxin and reducing the harm of mycotoxin to breeding industry.
基金Tangshan Science and Technology Planning Project(20150210C)Hebei Provincial Phase II Modern Agricultural Industry Technology System Innovation Team Building Project(HBCT2018120207,HBCT2018160403).
文摘In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.
文摘This paper proposes a voltage-based hot-spot detection method for defective cells in PV module using projector. The presence of internal crystal defects is one of the main causes of hot-spot phenomenon in PV modules. Authors previously investigated the physical characteristics of hot-spot phenomenon referring to internal crystal defect. Based on it, a hot-spot detection method named as current-based SRC （self reverse current） detection method is developed. However, it becomes extraordinarily complicated to determine the defective cells under low illumination. In order to avoid this disadvantage, authors improve the SRC detection method by applying voltage. From the feasibility experiment results, it is confirmed that by calculating cell HSI （hotspots index） with voltage, the PV modules with defective cells can be prospectively excluded even under low illumination.
文摘 Afour-month period of national special rectification for product quality and food safety officially started on August 25, and was focused on eight fields, including those of agricultural products and processed foods.……
基金Shenzhen High‐level Hospital Construction Fund,Grant/Award Number:G2022091National Key Research and Development Program of China,Grant/Award Numbers:2022YFC2304401,2022YFC2304402+2 种基金The Special Funds for Strategic Emerging Industry of Shenzhen,Grant/Award Number:F‐2022‐Z99‐502266Shenzhen Science and Technology Program,Grant/Award Number:RCBS20221008093104016Basic and Applied Basic Research Foundation of Guangdong Province,Grant/Award Number:2019A1515110119。
文摘Cancer immunotherapy has emerged as the fourth most prevalent approach to tumor treatment,alongside surgery,radiotherapy,and chemotherapy.After several decades of development,chimeric antigen receptor T(CAR-T)cell therapy,a promising branch of adoptive T-cell therapy,has demonstrated superior efficacy and safety in comparison to other cell therapies in the treatment of cancer.At present,CAR-T cells are predominantly used to treat hematological malignancies,although their application in solid tumors is being readily investigated.Although numerous studies have examined the biomarkers associated with the safety of CAR-T cell therapy,few have evaluated predictors of CAR-T cell therapeutic efficacy.Thus,the primary objective of this review article was to provide a comprehensive overview of the factors predicting the efficacy of CAR-T cell therapy,with a particular focus on biomarkers and their detection methods.
文摘A reverse-transcription loop-mediated isothermal amplification （RT-LAMP） method was established for the detection of wheat streak mosaic virus （WSMV）. Ac-cording to the conservative regions of the genes that encode the coat protein of WSMV, 2 pairs of primers were designed. Final y, the 1st pair of primers was select-ed through the specificity test. The sensitivity test showed the sensitivity of RT-LAMP method was 10 times higher than that of RT-PCR. In addition, the amplifica-tion of target gene could be judged visual y from the presence of fluorescence （cal-cein） in the final reaction system. The RT-LAMP method, established in this study, was rapid, easy, specific and sensitive. Moreover, it did not require sophisticated equip-ment. The RT-LAMP was suitable for the rapid detection of WSMV.
