Coronavirus 2019(COVID-19)is the current global buzzword,putting the world at risk.The pandemic’s exponential expansion of infected COVID-19 patients has challenged the medical field’s resources,which are already fe...Coronavirus 2019(COVID-19)is the current global buzzword,putting the world at risk.The pandemic’s exponential expansion of infected COVID-19 patients has challenged the medical field’s resources,which are already few.Even established nations would not be in a perfect position to manage this epidemic correctly,leaving emerging countries and countries that have not yet begun to grow to address the problem.These problems can be solved by using machine learning models in a realistic way,such as by using computer-aided images during medical examinations.These models help predict the effects of the disease outbreak and help detect the effects in the coming days.In this paper,Multi-Features Decease Analysis(MFDA)is used with different ensemble classifiers to diagnose the disease’s impact with the help of Computed Tomography(CT)scan images.There are various features associated with chest CT images,which help know the possibility of an individual being affected and how COVID-19 will affect the persons suffering from pneumonia.The current study attempts to increase the precision of the diagnosis model by evaluating various feature sets and choosing the best combination for better results.The model’s performance is assessed using Receiver Operating Characteristic(ROC)curve,the Root Mean Square Error(RMSE),and the Confusion Matrix.It is observed from the resultant outcome that the performance of the proposed model has exhibited better efficient.展开更多
The objective of the curtent study is to discuss the problems related to how data is used to calculate Yeare Lost with Disability (YLD) with the method recommended by the World Bank. The study includes collecting usef...The objective of the curtent study is to discuss the problems related to how data is used to calculate Yeare Lost with Disability (YLD) with the method recommended by the World Bank. The study includes collecting useful data, estimating disease duration and average age of disease onset,adjusting incidence and prevalence data by means of a software programme, DISMOD (Harvard University Incidence & Prevalence Model), and assessing the importance of YLD calculation for different diseases.Remission and fatality rates of 3 diseases were estimated by experts at 2 round consultations.Incidence rates, disease duration and average age of disease onset were calculated and adjusted by DISMOD. YLD due to schizothrenia is the highest among 4 diseases in two regions. YLD is 18. 88 % in disability adjusted life year for 4 diseases in Xiacheng District, and 19 .97 % in Fuyang County.Available data can be used for the calculation of YLD after being adjusted. DISMOD is a useful instrument to test the internal consistency of incidence, prevalence, rendssion and fatality rate. The adjusted data are acceptable to experts and DISMOD. To get rational remission and fatality rates, we can use a cohort method through expert consultations. To renect overall burden of disease, YLD calculation should be used.展开更多
Objective To screen coronaryartery disease (CAD) specific expressions and clone their genes. Method Blood samples were collected from CAD and non - CAD patients at the end of coronary angiography. mRNA from samples wa...Objective To screen coronaryartery disease (CAD) specific expressions and clone their genes. Method Blood samples were collected from CAD and non - CAD patients at the end of coronary angiography. mRNA from samples was isolated and converted into cDNA. After ligated with specific linkers, the cDNA was amplified with complementary primers. PCR products from CAD samples were named as tester; the ones from non - CAD samples were named as driver. With different ratio of tester to driver (1 : 100,1: 1, 000, and 1: 10, 000), they were mixed, denatured, and renatured. Single strand cD-NA was eliminated by Mung bean nuclease. Double strand cDNA presented only in tester was amplified, ligated in vector pUC19 and pUC53, and transformed into E. coll DH5a. Strains with inserted cDNA fragments were picked up based on blue and white selection. Insertions were screened by endonuclease digestion and DNA sequencing. Results were compared with DNA sequences of GeneBank. Results: After the selection with representational differential analysis, CAD specific cDNA fragments with different sizes (about 1kb, 0. 75kb, and 0. 6kb) were cloned. Among them, two fragments from unknown genes were identified. One presented a 43. 3 % similarity with part of the rattus norvegicus lipocortin gene. Another presented a 45. 4 % similarity with part of the human polynucleotide kinase 3' - phosphatase gene. Conclusion There are at least two CAD specific - ex- pressions from unknown genes that were partially similar to lipocortin and polynucleotide kinase 3'- phos-phatase genes, respectively. Expression of these genes might affect the formation and progression of plaque within coronary artery.展开更多
BACKGROUND The continuous development of social and economic progress and ongoing enhancement of infrastructure construction has led to drastic changes in the occurrence of trauma.AIM To analyze the epidemiological ch...BACKGROUND The continuous development of social and economic progress and ongoing enhancement of infrastructure construction has led to drastic changes in the occurrence of trauma.AIM To analyze the epidemiological characteristics of trauma in Lanzhou City to provide theoretical references for improving quality of trauma care.METHODS A retrospective analysis of clinical data from 16585 trauma patients treated at the First Hospital of Lanzhou University Trauma Center from November 1,2021 to October 31,2023 was conducted.Data including age,sex,time of trauma,cause of trauma,and major injured body parts were statistically analyzed.RESULTS A total of 18235 patients were admitted,with complete data for 16585 cases.Of these,9793 were male and 6792 were female(male-to-female ratio of 1.44:1).The peak times for trauma occurrence were 10 AM-12 PM and 6-10 PM,and the peak months were from May to October.The leading causes of trauma were falls(45.32%),other trauma(15.88%),road traffic accidents(15.15%),violence(10.82%),cutting/stabbing(9.41%),mechanical injuries(2.65%),winter sports injuries(0.36%),animal bites(0.22%),burns(0.09%),and electrical injuries(0.02%).The distribution of majorly injured body parts showed statistical significance,with limbs/skin being the most affected followed by the head/neck,chest/abdomen,and back.CONCLUSION Medical institutions and government agencies can implement preventive measures and policies based on the characteristics of trauma determined in this study to enhance the quality and level of trauma care.展开更多
DNA is a biological macromolecule that carries genetic information in organisms.It provides a series of predominant bio-logical advantages,such as sequence programmability,high biocompatibility,and low biotoxicity.As ...DNA is a biological macromolecule that carries genetic information in organisms.It provides a series of predominant bio-logical advantages,such as sequence programmability,high biocompatibility,and low biotoxicity.As such,it is a unique polymer material that shows great potential for application in biological and medical fields.DNA aptamers are short DNA sequences with a specific ability of molecular recognition.With its discovery,the application prospect of DNA materials has broadened,especially for the separation and analysis of biological particles.In this review,the functions and characteristics of DNA aptamers are introduced,and the applications of DNA materials in cell/exosome separation and cancer detection are summarized.The application prospect and possible challenges of DNA materials are predicted.展开更多
In recent years, Edwardsiella tarda has become one of the most deadly pathogens of Japanese fl ounder( Paralichthys olivaceus), causing serious annual losses in commercial production. In contrast to the rapid advances...In recent years, Edwardsiella tarda has become one of the most deadly pathogens of Japanese fl ounder( Paralichthys olivaceus), causing serious annual losses in commercial production. In contrast to the rapid advances in the aquaculture of P. o livaceus, the study of E. tarda resistance-related markers has lagged behind, hindering the development of a disease-resistant strain. Thus, a marker-trait association analysis was initiated, combining bulked segregant analysis(BSA) and quantitative trait loci(QTL) mapping. Based on 180 microsatellite loci across all chromosomes, 106 individuals from the F1333(♀: F0768 ×♂: F0915)(Nomenclature rule: F+year+family number) were used to detect simple sequence repeats(SSRs) and QTLs associated with E. tarda resistance. After a genomic scan, three markers(Scaffold 404-21589, Scaffold 404-21594 and Scaffold 270-13812) from the same linkage group(LG)-1 exhibited a signifi cant difference between DNA, pooled/bulked from the resistant and susceptible groups( P <0.001). Therefore, 106 individuals were genotyped using all the SSR markers in LG1 by single marker analysis. Two different analytical models were then employed to detect SSR markers with different levels of signifi cance in LG1, where 17 and 18 SSR markers were identifi ed, respectively. Each model found three resistance-related QTLs by composite interval mapping(CIM). These six QTLs, designated q E1–6, explained 16.0%–89.5% of the phenotypic variance. Two of the QTLs, q E-2 and q E-4, were located at the 66.7 c M region, which was considered a major candidate region for E. tarda resistance. This study will provide valuable data for further investigations of E. tarda resistance genes and facilitate the selective breeding of disease-resistant Japanese fl ounder in the future.展开更多
To confirm resistance and genetic rules of Xikemai 6 against physiological races of wheat stripe rust,physiological races CYR31,CYR32 and CYR33,Su11-4 and V26 were inoculated in Xikemai 6 and Mingxian 169 and their hy...To confirm resistance and genetic rules of Xikemai 6 against physiological races of wheat stripe rust,physiological races CYR31,CYR32 and CYR33,Su11-4 and V26 were inoculated in Xikemai 6 and Mingxian 169 and their hybrid progenies F_1,F_2 and F_3 at adult plant stage on March 2015. The results showed that the resistance of Xikemai 6 against CYR31 was controlled by 2 pairs of dominant genes and a pair of recessive genes; the resistance against CYR32 was controlled by three pairs of dominant resistant genes（ two pairs of genes performed cumulative effect）; the resistance against CYR33 was controlled by a pair of dominant genes and a pair of recessive genes; the resistance against Su11-4 was controlled by a pair of dominant genes and a pair of recessive genes independently or collaboratively; the resistance against V26 was controlled by a pair of dominant genes independently. Due to good performance of Xikemai 6 in test and production,as well as years of resistance identification and genetic analysis,Xikemai 6 was proved to be an excellent cultivar with good resistance against stripe rust,and the inheritance of its resistance was stable,so Xikemai 6 could be used as a germplasm resource and resistance material with excellent comprehensive character. Molecular marker and localization could be further studied,to provide new resistance parents for disease-resistant breeding of wheat.展开更多
Objective To comprehend the reiation of tobacco glycoprotein (TGP) to Buerger,s disease.Metbods TGP was isolated from crude tobacco leaves by basic immunologic techniques. Serum anti- TGPantibodies were tested by West...Objective To comprehend the reiation of tobacco glycoprotein (TGP) to Buerger,s disease.Metbods TGP was isolated from crude tobacco leaves by basic immunologic techniques. Serum anti- TGPantibodies were tested by Western blot analysis in 11 patients with Buerger,s disease, 15 healthy male smokers and11 nonsmoking healthy male subjects. Results 1. TGP is a dark brown protein of molecular weight 14000. It maybe a subunit of some high molecular weight protein, and exists in crude tobacco leaves. 2. Western blot analysisshowed that 81.81% of patients with Buerger’s disease (9/11), 33.33% of healthy smokers (5/15) and 27.27% ofhealthy nonsmokers (3/11) had serum anti- TGP antibodies. There was significant dtherence between patientswith Buerger,s disease and two control groups (P<0.05), and no signilicant dtherence between both control groups(P>0.05). Conclusion TGP does play an important role in the pathogenesis of Buerger’s disease. As anti - TGPantibodies are also found in some control subjects, it is speculated that other etiologic factors might coordinatelycontribute to the specifc vascular response to TGP in susceptible subjects.展开更多
Objective To estimate the lung cancer burden that may be attributable to ambient fine particulate matter （PM2.5） pollution in Guangzhou city in China from 2005 to 2013. Methods The data regarding PM2.5 exposure were...Objective To estimate the lung cancer burden that may be attributable to ambient fine particulate matter （PM2.5） pollution in Guangzhou city in China from 2005 to 2013. Methods The data regarding PM2.5 exposure were obtained from the ＆#39;Ambient air pollution exposure estimation for the Global Burden of Disease 2013＇ dataset at 0.1° ×0.1° spatial resolution. Disability-adjusted life years （DALYs） were estimated based on the information of mortality and incidence of lung cancer. Comparative risk analysis and integrated exposure-response function were used to estimate attributed disease burden. Results The population-weighted average concentration of PM2.5 was increased by 34.6% between 1990 and 2013, from 38.37 μg/m3 to 51.31 μg/m^3. The lung cancer DALYs in both men and women were increased by 36.2% from 2005 to 2013. The PM2.5 attributed lung cancer DALYs increased from 12105.0 （8181.0 for males and 3924.0 for females） in 2005 to 16489.3 （11291.7 for males and 5197.6 for females） in 2013. An average of 23.1% lung cancer burden was attributable to PM2.5 pollution in 2013. Conclusion PM2.5 has caused serious but under-appreciated public health burden in Guangzhou and the trend deteriorates. Effective strategies are needed to tackle this major public health problem.展开更多
The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction...The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction of colitis by intrarectal instillation of 2 mL of 4% （v/v） acetic acid solution. The degree of colonic mucosal damage was analyzed by examining mucosal damage, ulcer area, ulcer index and stool consistency. Intrarectal administration of 4% acetic acid resulted in significant modulation of serum alkaline phosphatase, lactate dehydrogenase, superoxide dismutase （SOD）, glutathione （GSH）, malondialdehyde （MDA） and myeloperoxidase （MPO） content along with colonic nitric oxide （NO）, xanthine oxidase （XO） level and protein carbonyl content in the colonic tissue as well as in blood. Naringin （40 and 80 mg/kg） exerted a dose dependent （P 〈 0.05） ameliorative effect, as it significantly increased hematological parameter as well as colonic SOD and GSH. There was a significant （P 〈 0.05） and dose dependant inhibition of macroscopical score, ulcer area along with colonic MDA, MPO activity by the 7 days of pretreatment of naringin （40 and 80 mg/kg）. Biochemical studies revealed a significant （P 〈 0.05） dose dependant inhibition in serum alkaline phosphatase （ALP） and lactate dehydrogenase （LDH） levels by pretreatment of naringin. Increased levels of colonic NO, XO, protein carbonyl content and DNA damage were also sig- nificantly decreased by naringin pretreatment. The findings of the present investigation propose that naringin has an anti-inflammatory, anti-oxidant and anti-apoptotic potential effect at colorectal sites as it modulates the production and expression of oxidative mediators such as MDA, MPO, NO and XO, thus reducing DNA damage.展开更多
Objective To identify the diffusion alterations of deep gray matter(GM)and white matter(WM)among Alzheimer’s disease(AD),mild cognitive impairment(MCI)and healthy people by atlas-based analysis(ABA),and to investigat...Objective To identify the diffusion alterations of deep gray matter(GM)and white matter(WM)among Alzheimer’s disease(AD),mild cognitive impairment(MCI)and healthy people by atlas-based analysis(ABA),and to investigate the respective relationship with cognitive function.Methods Twenty-one AD patients(AD group),8 MCI patients(MCI group)and展开更多
Objective To summarize the clinical of different racial patients with celiac disease(CD)and analyze the disease prevalence,diagnosis and treatment in Chinese population.Methods All the patients were diagnosed as CD an...Objective To summarize the clinical of different racial patients with celiac disease(CD)and analyze the disease prevalence,diagnosis and treatment in Chinese population.Methods All the patients were diagnosed as CD and enrolled in Beijing United Family Hospital between January 2005 and July 2015.Clinical data including nationality,age,symptoms,endoscopic and patho-展开更多
Chronic kidney disease （CKD） is a major public health problem that affects about 10% of the general population. Current approaches to characterize the category and progression of CKD are normally based on renal hist...Chronic kidney disease （CKD） is a major public health problem that affects about 10% of the general population. Current approaches to characterize the category and progression of CKD are normally based on renal histopathological results and clinical parameters. However, this information is not sufficient to predict CKD progression risk reliably or to guide preventive interventions. Nowadays, the appearance of systems biology has brought forward the concepts of ＂-omics＂ technologies, including genomics, transcriptomics, proteomics, and metabolomics. Systems biology, together with molecular analysis approaches such as microarray analysis, genome-wide association studies （GWAS）, and serial analysis of gene expression （SAGE）, has provided the framework for a comprehensive analysis of renal disease and serves as a starting point for generating novel molecular diagnostic tools for use in nephrology. In particular, analysis of urinary mRNA and protein levels is rapidly evolving as a non-invasive approach for CKD monitoring. All these systems biological molecular approaches are required for application of the concept of ＂personalized medicine＂ to progressive CKD, which will result in tailoring therapy for each patient, in contrast to the ＂one-size-fits-all＂ therapies currently in use.展开更多
Genome-wide association studies（GWASs）have revealed a plethora of putative susceptibility genes for Alzheimer＇s disease（AD）. With the sole exception of the APOE gene, these AD susceptibility genes have not been u...Genome-wide association studies（GWASs）have revealed a plethora of putative susceptibility genes for Alzheimer＇s disease（AD）. With the sole exception of the APOE gene, these AD susceptibility genes have not been unequivocally validated in independent studies. No single novel functional risk genetic variant has been identified. In this review, we evaluate recent GWASs of AD, and discuss their significance, limitations, and challenges in the investigation of the genetic spectrum of AD.展开更多
Foot-and-mouth disease virus(FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinaseL^(rop) of FMDV is involved in pathogenicity, and mutation of theL^(rop) SAP domain reduces FM...Foot-and-mouth disease virus(FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinaseL^(rop) of FMDV is involved in pathogenicity, and mutation of theL^(rop) SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containingL^(rop) with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L^(rop).展开更多
OBJECTIVE: To investigate the effectiveness of Shoushen granule,Chinese herbal preparation,on carotid artery elasticity in patients with carotid atherosclerosis.METHODS: The total of 156 carotid atherosclerosis patien...OBJECTIVE: To investigate the effectiveness of Shoushen granule,Chinese herbal preparation,on carotid artery elasticity in patients with carotid atherosclerosis.METHODS: The total of 156 carotid atherosclerosis patients were randomly divided into the intervention group(83 cases,treated with Shoushen granule) and the control group(73 cases,treated with pravastatin). Brachial-ankle pulse wave velocity(ba PWV) and Ankle-Brachial Pressure Index(ABI)were measured by automated arteriosclerosis detector. The changes of common carotid artery intima-media thickness(IMT) and parameters of the carotid artery elasticity in patients,including stiffness parameter(β),pressure-strain elastic modulus(Ep),arterial compliance(Ac),augmentation index(AI),and pulse wave velocity β(PWVβ) were detected by Echo-Tracking(ET) technique before and after 24 week treatment. In the meantime,levels of blood lipid,and liver and renal function were measured respectively.RESULTS: After 24 weeks,ba PWV,IMT and parameters of the carotid artery elasticity(β,Ep,AI and PWVβ) were markedly decreased in intervention group compared with those of before treatment(P < 0.01),but the level of Ac was increased significantly(P < 0.01). And there were no significant differences compared with control group on the same period(P > 0.05).CONCLUSION: In this pilot study,it was demonstrated ET technology and automated arteriosclerosis detector could be used to evaluate carotid artery elasticity effectively,and the action of Shoushen granule on carotid atherosclerosis might be related to the regulation of carotid artery elasticity.展开更多
This study modeled the spread of an influenza epidemic in the population of Oran, Algeria. We investigated the mathematical epidemic model, SEIR（Susceptible-Exposed-Infected-Removed）, through extensive simulations o...This study modeled the spread of an influenza epidemic in the population of Oran, Algeria. We investigated the mathematical epidemic model, SEIR（Susceptible-Exposed-Infected-Removed）, through extensive simulations of the effects of social network on epidemic spread in a Small World（SW） network, to understand how an influenza epidemic spreads through a human population. A combined SEIR-SW model was built, to help understand the dynamics of infectious disease in a community, and to identify the main characteristics of epidemic transmission and its evolution over time. The model was also used to examine social network effects to better understand the topological structure of social contact and the impact of its properties. Experiments were conducted to evaluate the combined SEIR-SW model. Simulation results were analyzed to explore how network evolution influences the spread of desease, and statistical tests were applied to validate the model. The model accurately replicated the dynamic behavior of the real influenza epidemic data, confirming that the susceptible size and topological structure of social networks in a human population significantly influence the spread of infectious diseases. Our model can provide health policy decision makers with a better understanding of epidemic spread,allowing them to implement control measures. It also provides an early warning of the emergence of influenza epidemics.展开更多
基金This work was supported by the Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia(Project no.GRANT 324).
文摘Coronavirus 2019(COVID-19)is the current global buzzword,putting the world at risk.The pandemic’s exponential expansion of infected COVID-19 patients has challenged the medical field’s resources,which are already few.Even established nations would not be in a perfect position to manage this epidemic correctly,leaving emerging countries and countries that have not yet begun to grow to address the problem.These problems can be solved by using machine learning models in a realistic way,such as by using computer-aided images during medical examinations.These models help predict the effects of the disease outbreak and help detect the effects in the coming days.In this paper,Multi-Features Decease Analysis(MFDA)is used with different ensemble classifiers to diagnose the disease’s impact with the help of Computed Tomography(CT)scan images.There are various features associated with chest CT images,which help know the possibility of an individual being affected and how COVID-19 will affect the persons suffering from pneumonia.The current study attempts to increase the precision of the diagnosis model by evaluating various feature sets and choosing the best combination for better results.The model’s performance is assessed using Receiver Operating Characteristic(ROC)curve,the Root Mean Square Error(RMSE),and the Confusion Matrix.It is observed from the resultant outcome that the performance of the proposed model has exhibited better efficient.
文摘The objective of the curtent study is to discuss the problems related to how data is used to calculate Yeare Lost with Disability (YLD) with the method recommended by the World Bank. The study includes collecting useful data, estimating disease duration and average age of disease onset,adjusting incidence and prevalence data by means of a software programme, DISMOD (Harvard University Incidence & Prevalence Model), and assessing the importance of YLD calculation for different diseases.Remission and fatality rates of 3 diseases were estimated by experts at 2 round consultations.Incidence rates, disease duration and average age of disease onset were calculated and adjusted by DISMOD. YLD due to schizothrenia is the highest among 4 diseases in two regions. YLD is 18. 88 % in disability adjusted life year for 4 diseases in Xiacheng District, and 19 .97 % in Fuyang County.Available data can be used for the calculation of YLD after being adjusted. DISMOD is a useful instrument to test the internal consistency of incidence, prevalence, rendssion and fatality rate. The adjusted data are acceptable to experts and DISMOD. To get rational remission and fatality rates, we can use a cohort method through expert consultations. To renect overall burden of disease, YLD calculation should be used.
文摘Objective To screen coronaryartery disease (CAD) specific expressions and clone their genes. Method Blood samples were collected from CAD and non - CAD patients at the end of coronary angiography. mRNA from samples was isolated and converted into cDNA. After ligated with specific linkers, the cDNA was amplified with complementary primers. PCR products from CAD samples were named as tester; the ones from non - CAD samples were named as driver. With different ratio of tester to driver (1 : 100,1: 1, 000, and 1: 10, 000), they were mixed, denatured, and renatured. Single strand cD-NA was eliminated by Mung bean nuclease. Double strand cDNA presented only in tester was amplified, ligated in vector pUC19 and pUC53, and transformed into E. coll DH5a. Strains with inserted cDNA fragments were picked up based on blue and white selection. Insertions were screened by endonuclease digestion and DNA sequencing. Results were compared with DNA sequences of GeneBank. Results: After the selection with representational differential analysis, CAD specific cDNA fragments with different sizes (about 1kb, 0. 75kb, and 0. 6kb) were cloned. Among them, two fragments from unknown genes were identified. One presented a 43. 3 % similarity with part of the rattus norvegicus lipocortin gene. Another presented a 45. 4 % similarity with part of the human polynucleotide kinase 3' - phosphatase gene. Conclusion There are at least two CAD specific - ex- pressions from unknown genes that were partially similar to lipocortin and polynucleotide kinase 3'- phos-phatase genes, respectively. Expression of these genes might affect the formation and progression of plaque within coronary artery.
基金Supported by Ministry of Science and Technology Senior Foreign Expert Program,No.G2019028023 and No.G2020028007National College Student Innovation and Entrepreneurship Program,No.202210730172+1 种基金Medical Innovation and Development Project of Lanzhou University,No.lzuyxcx-2022-99Joint Research Fund Project of Gansu Province,No.23JRRA1496.
文摘BACKGROUND The continuous development of social and economic progress and ongoing enhancement of infrastructure construction has led to drastic changes in the occurrence of trauma.AIM To analyze the epidemiological characteristics of trauma in Lanzhou City to provide theoretical references for improving quality of trauma care.METHODS A retrospective analysis of clinical data from 16585 trauma patients treated at the First Hospital of Lanzhou University Trauma Center from November 1,2021 to October 31,2023 was conducted.Data including age,sex,time of trauma,cause of trauma,and major injured body parts were statistically analyzed.RESULTS A total of 18235 patients were admitted,with complete data for 16585 cases.Of these,9793 were male and 6792 were female(male-to-female ratio of 1.44:1).The peak times for trauma occurrence were 10 AM-12 PM and 6-10 PM,and the peak months were from May to October.The leading causes of trauma were falls(45.32%),other trauma(15.88%),road traffic accidents(15.15%),violence(10.82%),cutting/stabbing(9.41%),mechanical injuries(2.65%),winter sports injuries(0.36%),animal bites(0.22%),burns(0.09%),and electrical injuries(0.02%).The distribution of majorly injured body parts showed statistical significance,with limbs/skin being the most affected followed by the head/neck,chest/abdomen,and back.CONCLUSION Medical institutions and government agencies can implement preventive measures and policies based on the characteristics of trauma determined in this study to enhance the quality and level of trauma care.
文摘DNA is a biological macromolecule that carries genetic information in organisms.It provides a series of predominant bio-logical advantages,such as sequence programmability,high biocompatibility,and low biotoxicity.As such,it is a unique polymer material that shows great potential for application in biological and medical fields.DNA aptamers are short DNA sequences with a specific ability of molecular recognition.With its discovery,the application prospect of DNA materials has broadened,especially for the separation and analysis of biological particles.In this review,the functions and characteristics of DNA aptamers are introduced,and the applications of DNA materials in cell/exosome separation and cancer detection are summarized.The application prospect and possible challenges of DNA materials are predicted.
基金Supported by the National Natural Science Foundation of China(No.31461163005)the Taishan Scholar Project of Shandong Province
文摘In recent years, Edwardsiella tarda has become one of the most deadly pathogens of Japanese fl ounder( Paralichthys olivaceus), causing serious annual losses in commercial production. In contrast to the rapid advances in the aquaculture of P. o livaceus, the study of E. tarda resistance-related markers has lagged behind, hindering the development of a disease-resistant strain. Thus, a marker-trait association analysis was initiated, combining bulked segregant analysis(BSA) and quantitative trait loci(QTL) mapping. Based on 180 microsatellite loci across all chromosomes, 106 individuals from the F1333(♀: F0768 ×♂: F0915)(Nomenclature rule: F+year+family number) were used to detect simple sequence repeats(SSRs) and QTLs associated with E. tarda resistance. After a genomic scan, three markers(Scaffold 404-21589, Scaffold 404-21594 and Scaffold 270-13812) from the same linkage group(LG)-1 exhibited a signifi cant difference between DNA, pooled/bulked from the resistant and susceptible groups( P <0.001). Therefore, 106 individuals were genotyped using all the SSR markers in LG1 by single marker analysis. Two different analytical models were then employed to detect SSR markers with different levels of signifi cance in LG1, where 17 and 18 SSR markers were identifi ed, respectively. Each model found three resistance-related QTLs by composite interval mapping(CIM). These six QTLs, designated q E1–6, explained 16.0%–89.5% of the phenotypic variance. Two of the QTLs, q E-2 and q E-4, were located at the 66.7 c M region, which was considered a major candidate region for E. tarda resistance. This study will provide valuable data for further investigations of E. tarda resistance genes and facilitate the selective breeding of disease-resistant Japanese fl ounder in the future.
基金Supported by Key Research Project of Wheat Breeding in Sichuan Province(2011NZ0098-3-18)
文摘To confirm resistance and genetic rules of Xikemai 6 against physiological races of wheat stripe rust,physiological races CYR31,CYR32 and CYR33,Su11-4 and V26 were inoculated in Xikemai 6 and Mingxian 169 and their hybrid progenies F_1,F_2 and F_3 at adult plant stage on March 2015. The results showed that the resistance of Xikemai 6 against CYR31 was controlled by 2 pairs of dominant genes and a pair of recessive genes; the resistance against CYR32 was controlled by three pairs of dominant resistant genes（ two pairs of genes performed cumulative effect）; the resistance against CYR33 was controlled by a pair of dominant genes and a pair of recessive genes; the resistance against Su11-4 was controlled by a pair of dominant genes and a pair of recessive genes independently or collaboratively; the resistance against V26 was controlled by a pair of dominant genes independently. Due to good performance of Xikemai 6 in test and production,as well as years of resistance identification and genetic analysis,Xikemai 6 was proved to be an excellent cultivar with good resistance against stripe rust,and the inheritance of its resistance was stable,so Xikemai 6 could be used as a germplasm resource and resistance material with excellent comprehensive character. Molecular marker and localization could be further studied,to provide new resistance parents for disease-resistant breeding of wheat.
文摘Objective To comprehend the reiation of tobacco glycoprotein (TGP) to Buerger,s disease.Metbods TGP was isolated from crude tobacco leaves by basic immunologic techniques. Serum anti- TGPantibodies were tested by Western blot analysis in 11 patients with Buerger,s disease, 15 healthy male smokers and11 nonsmoking healthy male subjects. Results 1. TGP is a dark brown protein of molecular weight 14000. It maybe a subunit of some high molecular weight protein, and exists in crude tobacco leaves. 2. Western blot analysisshowed that 81.81% of patients with Buerger’s disease (9/11), 33.33% of healthy smokers (5/15) and 27.27% ofhealthy nonsmokers (3/11) had serum anti- TGP antibodies. There was significant dtherence between patientswith Buerger,s disease and two control groups (P<0.05), and no signilicant dtherence between both control groups(P>0.05). Conclusion TGP does play an important role in the pathogenesis of Buerger’s disease. As anti - TGPantibodies are also found in some control subjects, it is speculated that other etiologic factors might coordinatelycontribute to the specifc vascular response to TGP in susceptible subjects.
基金supported by the Centre for Health Statistics Information,National Health and Family Planning Commission of the People’s Republic of China
文摘Objective To estimate the lung cancer burden that may be attributable to ambient fine particulate matter （PM2.5） pollution in Guangzhou city in China from 2005 to 2013. Methods The data regarding PM2.5 exposure were obtained from the ＆#39;Ambient air pollution exposure estimation for the Global Burden of Disease 2013＇ dataset at 0.1° ×0.1° spatial resolution. Disability-adjusted life years （DALYs） were estimated based on the information of mortality and incidence of lung cancer. Comparative risk analysis and integrated exposure-response function were used to estimate attributed disease burden. Results The population-weighted average concentration of PM2.5 was increased by 34.6% between 1990 and 2013, from 38.37 μg/m3 to 51.31 μg/m^3. The lung cancer DALYs in both men and women were increased by 36.2% from 2005 to 2013. The PM2.5 attributed lung cancer DALYs increased from 12105.0 （8181.0 for males and 3924.0 for females） in 2005 to 16489.3 （11291.7 for males and 5197.6 for females） in 2013. An average of 23.1% lung cancer burden was attributable to PM2.5 pollution in 2013. Conclusion PM2.5 has caused serious but under-appreciated public health burden in Guangzhou and the trend deteriorates. Effective strategies are needed to tackle this major public health problem.
文摘The aim of this study was to evaluate the effect of naringin on experimentally induced inflammatory bowel dis- ease in rats. Naringin （20, 40 and 80 mg/kg） was given orally for 7 days to Wistar rats before induction of colitis by intrarectal instillation of 2 mL of 4% （v/v） acetic acid solution. The degree of colonic mucosal damage was analyzed by examining mucosal damage, ulcer area, ulcer index and stool consistency. Intrarectal administration of 4% acetic acid resulted in significant modulation of serum alkaline phosphatase, lactate dehydrogenase, superoxide dismutase （SOD）, glutathione （GSH）, malondialdehyde （MDA） and myeloperoxidase （MPO） content along with colonic nitric oxide （NO）, xanthine oxidase （XO） level and protein carbonyl content in the colonic tissue as well as in blood. Naringin （40 and 80 mg/kg） exerted a dose dependent （P 〈 0.05） ameliorative effect, as it significantly increased hematological parameter as well as colonic SOD and GSH. There was a significant （P 〈 0.05） and dose dependant inhibition of macroscopical score, ulcer area along with colonic MDA, MPO activity by the 7 days of pretreatment of naringin （40 and 80 mg/kg）. Biochemical studies revealed a significant （P 〈 0.05） dose dependant inhibition in serum alkaline phosphatase （ALP） and lactate dehydrogenase （LDH） levels by pretreatment of naringin. Increased levels of colonic NO, XO, protein carbonyl content and DNA damage were also sig- nificantly decreased by naringin pretreatment. The findings of the present investigation propose that naringin has an anti-inflammatory, anti-oxidant and anti-apoptotic potential effect at colorectal sites as it modulates the production and expression of oxidative mediators such as MDA, MPO, NO and XO, thus reducing DNA damage.
文摘Objective To identify the diffusion alterations of deep gray matter(GM)and white matter(WM)among Alzheimer’s disease(AD),mild cognitive impairment(MCI)and healthy people by atlas-based analysis(ABA),and to investigate the respective relationship with cognitive function.Methods Twenty-one AD patients(AD group),8 MCI patients(MCI group)and
文摘Objective To summarize the clinical of different racial patients with celiac disease(CD)and analyze the disease prevalence,diagnosis and treatment in Chinese population.Methods All the patients were diagnosed as CD and enrolled in Beijing United Family Hospital between January 2005 and July 2015.Clinical data including nationality,age,symptoms,endoscopic and patho-
文摘Chronic kidney disease （CKD） is a major public health problem that affects about 10% of the general population. Current approaches to characterize the category and progression of CKD are normally based on renal histopathological results and clinical parameters. However, this information is not sufficient to predict CKD progression risk reliably or to guide preventive interventions. Nowadays, the appearance of systems biology has brought forward the concepts of ＂-omics＂ technologies, including genomics, transcriptomics, proteomics, and metabolomics. Systems biology, together with molecular analysis approaches such as microarray analysis, genome-wide association studies （GWAS）, and serial analysis of gene expression （SAGE）, has provided the framework for a comprehensive analysis of renal disease and serves as a starting point for generating novel molecular diagnostic tools for use in nephrology. In particular, analysis of urinary mRNA and protein levels is rapidly evolving as a non-invasive approach for CKD monitoring. All these systems biological molecular approaches are required for application of the concept of ＂personalized medicine＂ to progressive CKD, which will result in tailoring therapy for each patient, in contrast to the ＂one-size-fits-all＂ therapies currently in use.
基金supported by CHINACANADA Joint Initiative on Alzheimer’s Disease and Related Disorders(81261120571)the National Basic Research Development Program(973 Program)of China(2011CB504104)+6 种基金Scientific Promoting Project of Beijing Institute for Brain Disorders(BIBDPXM2014_014226_000016)Seed Grant of International Alliance of Translational Neuroscience(PXM2014_014226_000006)Key Medical Professional Development Plan of Beijing Municipal Administration of Hospitals(ZYLX201301)the National Science and Technology Major Project for‘‘Major New Drug Innovation and Development’’of the Twelfth 5-year Plan Period of China(2011ZX09307-001-03)the Major Project of the Science and Technology Plan of the Beijing Municipal Science&Technology Commission of China(D111107003111009)the National Key Technology R&D Program in the Eleventh Five-year Plan Period of China(2006BAI02B01)the Key Project of the National Natural Science Foundation of China(30830045)
文摘Genome-wide association studies（GWASs）have revealed a plethora of putative susceptibility genes for Alzheimer＇s disease（AD）. With the sole exception of the APOE gene, these AD susceptibility genes have not been unequivocally validated in independent studies. No single novel functional risk genetic variant has been identified. In this review, we evaluate recent GWASs of AD, and discuss their significance, limitations, and challenges in the investigation of the genetic spectrum of AD.
基金supported by grants from the National Science and Technology Ministry (2015BAD12B04)National Natural Sciences Foundation of China (No.31302118,31502042 and 31402179)+2 种基金the Gansu Science Foundation for Distinguished Young Scholars (no.145RJDA328)the International Atomic Energy Agency (16025/R0)the Key technologies R&Dprogram of Gansu Province (1302NKDA027)
文摘Foot-and-mouth disease virus(FMDV) is the causative agent of a highly contagious disease in livestock. The viral proteinaseL^(rop) of FMDV is involved in pathogenicity, and mutation of theL^(rop) SAP domain reduces FMDV pathogenicity in pigs. To determine the gene expression profiles associated with decreased pathogenicity in porcine cells, we performed transcriptome analysis using next-generation sequencing technology and compared differentially expressed genes in SK6 cells infected with FMDV containingL^(rop) with either a wild-type or mutated version of the SAP domain. This analysis yielded 1,853 genes that exhibited a ≥ 2-fold change in expression and was validated by real-time quantitative PCR detection of several differentially expressed genes. Many of the differentially expressed genes correlated with antiviral responses corresponded to genes associated with transcription factors, immune regulation, cytokine production, inflammatory response, and apoptosis. Alterations in gene expression profiles may be responsible for the variations in pathogenicity observed between the two FMDV variants. Our results provided genes of interest for the further study of antiviral pathways and pathogenic mechanisms related to FMDV L^(rop).
基金Supported by the National Natural Science Foundation of China(the Role of TLR4/My D88/NF-κB Signal Transduction Pathway and Expression of mi RNA-146a in Atherosclerosis and the Intervention Mechanism of Shen Invigorating Compounds,No.81202731Study on the mechanism of two-ways regulation of angiogenesis using Guanxin 1 from the VEGF-Ang cascade signaling pathway,No.81202664)+2 种基金General Program of Shanghai Municipal Health Bureau Foundation(Evaluation of the Clinical Effect of the Shoushen Granule in Patients with Atherosclerosis based on Echo-Tracking Technique,No.20114047Study on Biomarkers of Atherosclerosis based on Telomere,No.2010225)Traditional Chinese Medicine Foundation of Shanghai Municipal Health Bureau(Anti atherosclerosis Study of the Traditional Chinese Medicine Shoushen Granule on the Basis of Telomere,No.2010L046A)
文摘OBJECTIVE: To investigate the effectiveness of Shoushen granule,Chinese herbal preparation,on carotid artery elasticity in patients with carotid atherosclerosis.METHODS: The total of 156 carotid atherosclerosis patients were randomly divided into the intervention group(83 cases,treated with Shoushen granule) and the control group(73 cases,treated with pravastatin). Brachial-ankle pulse wave velocity(ba PWV) and Ankle-Brachial Pressure Index(ABI)were measured by automated arteriosclerosis detector. The changes of common carotid artery intima-media thickness(IMT) and parameters of the carotid artery elasticity in patients,including stiffness parameter(β),pressure-strain elastic modulus(Ep),arterial compliance(Ac),augmentation index(AI),and pulse wave velocity β(PWVβ) were detected by Echo-Tracking(ET) technique before and after 24 week treatment. In the meantime,levels of blood lipid,and liver and renal function were measured respectively.RESULTS: After 24 weeks,ba PWV,IMT and parameters of the carotid artery elasticity(β,Ep,AI and PWVβ) were markedly decreased in intervention group compared with those of before treatment(P < 0.01),but the level of Ac was increased significantly(P < 0.01). And there were no significant differences compared with control group on the same period(P > 0.05).CONCLUSION: In this pilot study,it was demonstrated ET technology and automated arteriosclerosis detector could be used to evaluate carotid artery elasticity effectively,and the action of Shoushen granule on carotid atherosclerosis might be related to the regulation of carotid artery elasticity.
文摘This study modeled the spread of an influenza epidemic in the population of Oran, Algeria. We investigated the mathematical epidemic model, SEIR（Susceptible-Exposed-Infected-Removed）, through extensive simulations of the effects of social network on epidemic spread in a Small World（SW） network, to understand how an influenza epidemic spreads through a human population. A combined SEIR-SW model was built, to help understand the dynamics of infectious disease in a community, and to identify the main characteristics of epidemic transmission and its evolution over time. The model was also used to examine social network effects to better understand the topological structure of social contact and the impact of its properties. Experiments were conducted to evaluate the combined SEIR-SW model. Simulation results were analyzed to explore how network evolution influences the spread of desease, and statistical tests were applied to validate the model. The model accurately replicated the dynamic behavior of the real influenza epidemic data, confirming that the susceptible size and topological structure of social networks in a human population significantly influence the spread of infectious diseases. Our model can provide health policy decision makers with a better understanding of epidemic spread,allowing them to implement control measures. It also provides an early warning of the emergence of influenza epidemics.
