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Microplastic exposure disturbs sleep structure,reduces lifespan,and decreases ovary size in Drosophila melanogaster
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作者 Wei Yan Zi-Jie Li +5 位作者 Zi-Yi Lin Shu-Qin Ji William Ka Fai Tse Zhi-Qiang Meng Chang Liu Lei Li 《Zoological Research》 SCIE CSCD 2024年第4期805-820,共16页
The organ-specific toxicity resulting from microplastic(MP)exposure has been extensively explored,particularly concerning the gut,liver,testis,and lung.However,under natural conditions,these effects are not restricted... The organ-specific toxicity resulting from microplastic(MP)exposure has been extensively explored,particularly concerning the gut,liver,testis,and lung.However,under natural conditions,these effects are not restricted to specific organs or tissues.Investigating whether MP exposure presents a systemic threat to an entire organism,impacting factors such as lifespan,sleep,and fecundity,is essential.In this study,we investigated the effects of dietary exposure to two different doses of MPs(1–5μm)using the terrestrial model organism Drosophila melanogaster.Results indicated that the particles caused gut damage and remained within the digestive system.Continuous MP exposure significantly shortened the lifespan of adult flies.Even short-term exposure disrupted sleep patterns,increasing the length of daytime sleep episodes.Additionally,one week of MP exposure reduced ovary size,with a trend towards decreased egg-laying in mated females.Although MPs did not penetrate the brain or ovaries,transcriptome analysis revealed altered gene expression in these tissues.In the ovary,Gene Ontology(GO)analysis indicated genotoxic effects impacting inflammation,circadian regulation,and metabolic processes,with significant impacts on extracellular structure-related pathways.In the brain,GO analysis identified changes in pathways associated with proteolysis and carbohydrate metabolism.Overall,this study provides compelling evidence of the systemic negative effects of MP exposure,highlighting the urgent need to address and mitigate environmental MP pollution. 展开更多
关键词 Microplastics SLEEP LIFESPAN REPRODUCTION Risk assessment drosophila melanogaster
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Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy
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作者 Hongyu Pan Qianwang Zheng +6 位作者 Yuan Zou Guozhao Luo Mingzhen Tu Na Wang Jingru Zhong Liqiong Guo Junfang Lin 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第6期3434-3446,共13页
Ergothioneine is a natural amino acid with powerful antioxidant activity.Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans,and enhanc... Ergothioneine is a natural amino acid with powerful antioxidant activity.Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans,and enhance telomerase activity.In present study,the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies.And the alterations in the gut barrier,gut morphology,and other gut physiological indicators of aging flies were evaluated.Here,the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life,and significant alterations in the gut microbiota populations were only observed in flies at late-life.And ergothioneine attenuated age-related gut injuries,including decreasing reactive oxygen species levels in intestinal enterocytes,reduction of intestinal cells death,and preservation of gut integrity in aging flies.Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy.And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism. 展开更多
关键词 ERGOTHIONEINE Gut microbiota Intestinal autophagy Intestinal barrier drosophila melanogaster
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Naringin ameliorates H_(2)O_(2)-induced oxidative damage in cells and prolongs the lifespan of female Drosophila melanogaster via the insulin signaling pathway
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作者 Xiaomei Du Kexin Wang +7 位作者 Xiaoyan Sang Xiangxing Meng Jiao Xie Tianxin Wang Xiaozhi Liu Qun Huang Nan Zhang Hao Wang 《Food Science and Human Wellness》 SCIE CSCD 2024年第3期1231-1245,共15页
Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the an... Naringin exists in a wide range of Chinese herbal medicine and has proven to possess several pharmacological properties.In this study,PC12,HepG2 cells,and female Drosophila melanogaster were used to investigate the antioxidative and anti-aging effects of naringin and explore the underlying mechanisms.The results showed that naringin inhibited H_(2)O_(2)-induced decline in cell viability and decreased,the content of reactive oxygen species in cells.Meanwhile,naringin prolonged the lifespan of flies,enhanced the abilities of climbing and the resistance to stress,improved the activities of antioxidant enzymes,and decreased malondialdehyde content.Naringin also improved intestinal barrier dysfunction and reduced abnormal proliferation of intestinal stem cells.Moreover,naringin down-regulated the mRNA expressions of inr,chico,pi 3k,and akt-1,and up-regulated the mRNA expressions of dilp2,dilp3,dilp5,and foxo,thereby activating autophagy-related genes and increasing the number of lysosomes.Furthermore,the mutant stocks assays and computer molecular simulation results further indicated that naringin delayed aging by inhibiting the insulin signaling(IIS)pathway and activating the autophagy pathway,which was consistent with the result of network pharmacological predictions. 展开更多
关键词 drosophila melanogaster Insulin signaling(IIS)pathway NARINGIN PC12 cell HepG2 cell
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Effects of Sodium Cyclohexyl Sulfamate on Growth and Development of Drosophila melanogaster
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作者 Lei WEI Danni MA +2 位作者 Guobao JIANG Jiayu WU Na FENG 《Agricultural Biotechnology》 2024年第4期65-69,共5页
[Objectives]This study was conducted to investigate the effects of different concentrations of sodium cyclohexyl sulfamate on the growth and development of Drosophila melanogaster.[Methods]Different concentrations of ... [Objectives]This study was conducted to investigate the effects of different concentrations of sodium cyclohexyl sulfamate on the growth and development of Drosophila melanogaster.[Methods]Different concentrations of sodium cyclohexyl sulfamate were added to the culture medium,and the effects of different concentrations of sodium cyclohexyl sulfamate on the development time and weight of D.melanogaster in various life stages were statistically analyzed.[Results]High concentration of sodium cyclohexyl sulfamate delayed the time of pupation and eclosion of D.melanogaster,which made D.melanogaster lose weight.The number of male D.melanogaster in the first generation was much larger than that of female individuals,which indicated that the effect of sodium cyclohexyl sulfamate on male D.melanogaster was greater than that of female individuals.In a word,high concentration of sodium cyclohexyl sulfamate significantly inhibited the growth and development of D.melanogaster.[Conclusions]This study provides some reference data for the research perspective of food additives and the safe use of sodium cyclohexyl sulfamate. 展开更多
关键词 drosophila melanogaster Sodium cyclohexyl sulfamate PUPATION ECLOSION
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Research on the Karyotype and Evolution of Drosophila melanogaster Species Group 被引量:1
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作者 邓秋红 曾庆韬 +2 位作者 钱远槐 李春选 杨勇 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2007年第3期196-213,共18页
Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationsh... Mitotic metaphase chromosomes of 34 species of Drosophila melanogaster species group were examined. Certain new karyotypes were described for the first time, and their evolutionary and interspecific genetic relationships among 8 subgroups of D. melanogaster species group were analyzed systematically. The results were as follows. The basic karyotype of elegans subgroup was type A. The karyotypes of eugracilis subgroup, melanogaster subgroup, and ficusphila subgroup were all type C. The karyotypes of takahashii subgroup and suzukii subgroup were both type C and type D. The montium subgroup had six kinds of karyotypes types B, C, C', D, D', and E. The ananassae subgroup had three kinds of karyotypes: types F, G, and H. Thus, the melanogaster species group was classified into five pedigrees based on the diversity of these karyotypes: 1) elegans; 2) eugracilis-melanogasterficusphila; 3) takkahashii-suzukii; 4) montium; 5) ananassae. The above-mentioned results in karyotypic evolution were consistent with those of DNA sequence analysis reported by Yang except for the elegans subgroup and this subgroup was considered as the ancestral subgroup. Karyotype analysis of the same drosophila from different isofemale lines indicated that the same Drosophila from different places showed karyotypic variation which might be due to different geographical environment and evolutionary degree or interaction between the two factors. 展开更多
关键词 drosophila drosophila melanogaster species group KARYOTYPE genetic relationships EVOLUTION
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Simulation of gene pyramiding in Drosophila melanogaster 被引量:4
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作者 Li Jiang Fuping Zhao Qin Zhang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第12期737-742,共6页
Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated. But it has not been used in animal breeding, ... Gene pyramiding has been successfully practiced in plant breeding for developing new breeds or lines in which favorable genes from several different lines were integrated. But it has not been used in animal breeding, and some theoretical investigation and simulation analysis with respect to its strategies, feasibility and efficiency are needed before it can be implemented in animals. In this study, we used four different pure lines of Drosophila melanogaster, each of which is homozygous at a specific mutant gene with a visible effect on phenotype, to simulate the gene pyramiding process and analyze the duration and population size required in different pyramiding strategies. We finally got the ideal individuals, which are homozygous at the four target genes simultaneously. This study demonstrates that gene pyramiding is feasible in animal breeding and the interaction between genes may affect the final results. 展开更多
关键词 gene pyramiding drosophila melanogaster SIMULATION
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黑腹果蝇(Drosophila melanogaster)视觉系统显微结构的研究 被引量:1
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作者 暴学祥 李伟红 +4 位作者 李霞 牛华 李一娜 田喜梅 马创 《东北师大学报(自然科学版)》 CAS CSCD 北大核心 2004年第1期71-77,共7页
 采用CP(Colophony-Paraffin,CP,专利号:ZL98125709.7)切片和定向包埋技术,首次对黑腹果蝇(Drosophilamelanogaster)的头部进行了3μm的连续切片及制片,经常规染色后,在光学显微镜下对其视觉系统的显微结构进行了详细的观察研究.结果表...  采用CP(Colophony-Paraffin,CP,专利号:ZL98125709.7)切片和定向包埋技术,首次对黑腹果蝇(Drosophilamelanogaster)的头部进行了3μm的连续切片及制片,经常规染色后,在光学显微镜下对其视觉系统的显微结构进行了详细的观察研究.结果表明,黑腹果蝇的视觉系统主要由复眼、视叶和一些纤维通路组成.复眼由许多形态相同的小眼构成,每个小眼由外向内分别由半球形的角膜、倒锥形的晶体和柱形的视觉柱组成,视觉柱中有7个感光细胞,其轴突穿过复眼底部的视神经孔与视叶相连;视叶由外向内又依次分为神经节层、外髓、内髓板和内髓4个髓质区以及视网膜底部与神经节层之间的网膜后交叉、神经节层与外髓之间的外交叉和外髓与内髓之间的内交叉.黑腹果蝇视觉系统中主要包括角膜细胞、晶锥细胞、网膜细胞(光感细胞)、色素细胞、柱状神经元、无长突细胞和切向神经元7种类型的神经细胞,其胞体直径均小于3μm. 展开更多
关键词 黑腹果蝇 视觉系统 显微结构
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裁翅对果蝇(Drosophila melanogaster)生殖行为的影响 被引量:3
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作者 张婷婷 张霞 《石河子大学学报(自然科学版)》 CAS 2005年第5期580-582,共3页
选用野生型、残翅和白眼3种不同基因型的果蝇,人为地将野生型和白眼果蝇的翅膀分别剪去0/3,1/3,1/2,2/3,1/1,设计39种不同的杂交组合,培养在相同大小的容器中,置于室温条件下,统计各种组合的子代果蝇发生量。结果显示:果蝇的生殖过程中... 选用野生型、残翅和白眼3种不同基因型的果蝇,人为地将野生型和白眼果蝇的翅膀分别剪去0/3,1/3,1/2,2/3,1/1,设计39种不同的杂交组合,培养在相同大小的容器中,置于室温条件下,统计各种组合的子代果蝇发生量。结果显示:果蝇的生殖过程中,不同性状的个体之间存在一定的生殖“歧视”,同时进入繁殖期时,翅是两性吸引的重要的结构部分,雄性主要依靠振翅的频率来吸引异性,因此果蝇的翅是繁殖交尾的一种关键辅助结构。 展开更多
关键词 果蝇 裁翅 生殖行为
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Ganoderma lucidum spore oil(GLSO),a novel antioxidant,extends the average life span in Drosophila melanogaster 被引量:5
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作者 Yi Zhang Hongfei Cai +5 位作者 Zhu Tao Cheng Yuan Zhaojian Jiang Juyan Liu Hiroshi Kurihara Wendong Xu 《Food Science and Human Wellness》 SCIE 2021年第1期38-44,共7页
In ancient China,Ganoderma lucidum was believed to be a medical fungus that could increase lifespan.Recently,pharmacologic studies have found that polysaccharide peptides and triterpenoids extracted from Ganoderma luc... In ancient China,Ganoderma lucidum was believed to be a medical fungus that could increase lifespan.Recently,pharmacologic studies have found that polysaccharide peptides and triterpenoids extracted from Ganoderma lucidum have various physiological effects as active compounds.However,the effects of spore oil isolated from Ganoderma lucidum remains unknown.In this study,the biological effects of Ganoderma lucidum spore oil(GLSO)were evaluated using a Drosophila melanogaster model.Compared with untreated groups,groups treated with GLSO had significantly longer average and maximum lifespan in both normal conditions and under oxidative stress.The activities of various antioxidant enzymes were measured to determine the antioxidant effect of GLSO.GLSO treatment markedly enhanced total superoxide dismutase(SOD)and catalase(CAT)activity and decreased levels of malondialdehyde(MDA).Further,we found dose-dependent increases in the mRNA expression of Cu,Zn-SOD,Mn-SOD,and CAT in GLSO-treated groups.These results suggest that GLSO may effectively eliminate free radicals and extend lifespan in Drosophila.Future work should investigate the value of GLSO as a functional food for the prevention of aging in larger animal models. 展开更多
关键词 Ganoderma lucidum spore oil Antioxidant activity drosophila melanogaster ANTI-AGING
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Factors affecting mito-nuclear codon usage interactions in the OXPHOS system of Drosophila melanogaster 被引量:1
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作者 Zheng Sun Liang Ma +2 位作者 Robert W. Murphy Xiansheng Zhang Dawei Huang 《Journal of Genetics and Genomics》 SCIE CAS CSCD 北大核心 2008年第12期729-735,共7页
Codon usage bias varies considerably among genomes and even within the genes of the same genome. In eukaryotic organisms, energy production in the form of oxidative phosphorylation (OXPHOS) is the only process under... Codon usage bias varies considerably among genomes and even within the genes of the same genome. In eukaryotic organisms, energy production in the form of oxidative phosphorylation (OXPHOS) is the only process under control of both nuclear and mitochondrial genomes. Although factors affecting codon usage in a single genome have been studied, this has not occurred when both interactional genomes are involved. Consequently, we investigated whether or not other factors influence codon usage of coevolved genes. We used Drosophila melanogaster as a model organism. Our χ^2 test on the number of codons of nuclear and mitochondrial genes involved in the OXPHOS system was significantly different (χ^2= 7945.16, P 〈 0.01). A plot of effective number of codons against GC3s content of nuclear genes showed that few genes lie on the expected curve, indicating that codon usage was random. Correspondence analysis indicated a significant correlation between axis 1 and codon adaptation index (R = 0.947, P 〈 0.01) in every nuclear gene sequence. Thus, codon usage bias of nuclear genes appeared to be affected by translational selection. Correlation between axis 1 coordinates and GC content (R = 0.814, P 〈 0.01) indicated that the codon usage of nuclear genes was also affected by GC composition. Analysis of mitochondrial genes did not reveal a significant correlation between axis 1 and any parameter. Statistical analyses indicated that codon usages of both nDNA and mtDNA were subjected to context-dependent mutations. 展开更多
关键词 drosophila melanogaster oxidative phosphorylation NDNA MTDNA codon usage
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Stability and Refolding of Prophenol Oxidase Protein with 2-Propanol in Drosophila melanogaster 被引量:1
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作者 Eri Sato Kotomi Mita Nobuhiko Asada 《Journal of Life Sciences》 2012年第8期952-956,共5页
Phenol oxidase in Drosophila melanogaster occurs as folded phase precursors designated as prophenol oxidase A1 and A3, and prophenol oxidase is activated with alcohol, especially 2-propanol, within a few minutes as un... Phenol oxidase in Drosophila melanogaster occurs as folded phase precursors designated as prophenol oxidase A1 and A3, and prophenol oxidase is activated with alcohol, especially 2-propanol, within a few minutes as unfolded-phase in vitro. To clarify a common effect of alcohols on proteins and peptides, the extract containing prophenol oxidase protein was prepared. Phenol oxidase activity activated with 2-propanol has been maintained stable at least 24 hours remains as it is. Protein of prophenol oxidase was not denatured opposite hypnoses known as the instability of protein with alcohol. Activated prophenol oxidase with 2-propanol remain enzyme activity with no aggregation, stable, renaturation, and the refolding phenomena occurred around the active phase within the catalytic active center of prophenol oxidase protein in Drosophila melanogaster. This study is important to induce the wide range applications of the effect in many fields for rational drag design. 展开更多
关键词 STABILITY 2-propanol REFOLDING prophenol oxidase drosophila melanogaster.
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中国东北地区黑腹果蝇(Drosophila melanogaster)P因子分布和来源
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作者 胡广安 曾庆韬 +1 位作者 钱远槐 李守涛 《实验生物学报》 CSCD 北大核心 2003年第4期295-300,共6页
真核生物的转座因子(transposable elements)特别是果蝇P因子在研究生物进化上有重要的意义。以我国东北地区13个地方及毗邻的北京、烟台和呼和浩特三个地方共130个黑腹果蝇(D.melanogaster)单雌系为材料,对P因子序列的ORF2-ORF3区段进... 真核生物的转座因子(transposable elements)特别是果蝇P因子在研究生物进化上有重要的意义。以我国东北地区13个地方及毗邻的北京、烟台和呼和浩特三个地方共130个黑腹果蝇(D.melanogaster)单雌系为材料,对P因子序列的ORF2-ORF3区段进行PCR扩增,统计不同地方黑腹果蝇群体的P因子在此区段的缺失频率,再从整个地区来分析P因子缺失的分布规律,以推导东北地区黑腹果蝇中P因子的传递和扩散途径。结果显示P因子缺失频率由边境地区向内地逐渐递减,群体相对隔离的地方也较低,推断我国东北地区黑腹果蝇中P因子由朝鲜和俄罗斯向中国边境入侵后,逐步向中国内地扩散。 展开更多
关键词 中国 东北地区 黑腹果蝇 P因子 缺失频率 转座因子 起源
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三丁基锡对黑腹果蝇(Drosophila melanogaster)的致死效应与生殖阻碍
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作者 王云 许善超 +2 位作者 赵正平 童贯和 王顺昌 《淮南师范学院学报》 2010年第4期143-145,共3页
[目的]研究三丁基锡(Tributyltin,TBT)化合物对黑腹果蝇(Drosophila melanogaster)的致死效应和生殖阻碍作用。[方法]先进行TBT对黑腹果蝇的半致死剂量测定,再利用初次产卵时间、产卵数量和孵化率指标衡量TBT的生殖阻碍作用。[结果]TBT... [目的]研究三丁基锡(Tributyltin,TBT)化合物对黑腹果蝇(Drosophila melanogaster)的致死效应和生殖阻碍作用。[方法]先进行TBT对黑腹果蝇的半致死剂量测定,再利用初次产卵时间、产卵数量和孵化率指标衡量TBT的生殖阻碍作用。[结果]TBT对野生型黑腹果蝇的24h、48h、72h和96hLC50分别是441μM、270μM、220μM和191μM。随着TBT浓度的升高,野生型、残翅型和白眼型果蝇的初次产卵时间均呈现推延现象,并且产卵数量和孵化率也呈现递减趋势。[结论]黑腹果蝇对TBT具有高耐受力。TBT具有显著的生殖阻碍作用,必须禁止TBT的使用以降低生态危害。 展开更多
关键词 三丁基锡 黑腹果蝇 半致死剂量 生殖阻碍
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Observation of Biological Character of Drosophila melanogaster Meigen on Cherry and Screening of Its Insecticides
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作者 Li Deyou Chen Xiaojun +4 位作者 Li Chonghui Geng Kun Zhang Bin Hu Jifeng Yuan Jie 《Plant Diseases and Pests》 CAS 2013年第1期7-9,共3页
Indoor rearing observation and efficacy test of insecticides were conducted in 2010 to understand the biochemical characteristics of Drosophila rnelano-gaster and high efficient insecticides against the insect pest. T... Indoor rearing observation and efficacy test of insecticides were conducted in 2010 to understand the biochemical characteristics of Drosophila rnelano-gaster and high efficient insecticides against the insect pest. The results showed that D. melanogaster had preference to eight kinds of fruits with proliferation. Fe-male adults of D. melanogaster laid eggs under epidermis of cherry fruits and newly hatched larvae caused damage on fruits. When the temperatures were ( 18 ±2) ℃ and ( 23 ± 2 ) ℃, the egg stage was 2.4 - 3.0 and 1.2 - 1.8 d ; the larval stage was 11.2 - 12.2 and 5.6 - 6.5 d; the papal stage was 9.5 - 10.6 and 5.0 - 6.2 d ; one generation was 23.1 - 25.4 and 12.8 - 14.2 d, respectively. Four insecticides ( including 1% emamectin benzoate EC 3 000 times dilution, 90% crys- tal trichlorfon 1 000 times dilution, 40% phoxim EC 1 500 times dilution and 25% buprofezin WP 2 000 times dilution) had higher control effects against D. mela-nogaster, with mortalities of 85.00% - 100.00%, 96.67% - 100.00% and 100.00% after insecticide application for 1, 3 and 6 h, respectively. 展开更多
关键词 CHERRY drosophila melanogaster Meigen Biological character Insecticide screening
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Positive effect of porphyrans on the lifespan and vitality of Drosophila melanogaster
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作者 赵婷婷 张全斌 +1 位作者 綦慧敏 李智恩 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2007年第4期373-377,共5页
The effects of degraded porphyran (P1) and natural porphyran (P) on the lifespan and vitality of Drosophila melanogaster are studied. The porphyrans, added daily to the food medium at 0.2% and 1% concentrations, can s... The effects of degraded porphyran (P1) and natural porphyran (P) on the lifespan and vitality of Drosophila melanogaster are studied. The porphyrans, added daily to the food medium at 0.2% and 1% concentrations, can significantly increase the lifespan in average of 55.79 and 58.23 d in 0.2% P1 diet females and 1% P1 diet males, extending by 12.29% and 8.60% over the corresponding controls, respectively. The effects of porphyrans on D. melanogaster in heat-stress condition were also examined, and found a remarkable increase in survival time. The results which are consistently associated with the use of porphyrans are related to their free radical scavenger action. Considerable increase in vitality demonstrated that vitalities of middle-aged fly (assessed by measuring their mating capacity) was observed after porphyrans addition. Therefore, porphyrans are effective in reducing the rate of aging, and P1 in low molecular weight is better than natural P. 展开更多
关键词 drosophila melanogaster porphyrans LIFESPAN HEAT-STRESS mating capacity
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Effects of SPNE on Growth,Development and Correlated Traits of Five Drosophila melanogaster Strains
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作者 Bochun ZHOU Qifen DENG +2 位作者 Suqin YU Shaoming CHANG Daolian FENG 《Agricultural Biotechnology》 CAS 2014年第5期7-10,共4页
Five homozygous strains of Drosophila melanogaster were used as the experimental materials and treated with SPNE ( Special Plant Nutritional Element). D. melanogaster larvae were incubated at 18 ℃ in Drosophila cul... Five homozygous strains of Drosophila melanogaster were used as the experimental materials and treated with SPNE ( Special Plant Nutritional Element). D. melanogaster larvae were incubated at 18 ℃ in Drosophila culture medium with SPNE addition. The results showed that larva emergence, pupa emergence and adult emergence in SPNE treatment were earlier than that in control. SPNE treatment accelerated the growth and development, shortened the life cycle, significantly increased the quantity of offspring and slightly improved the weight of D. melanogaster, which confirmed that SPNE posed significant effects on D. melanogaster. This study laid foundation for the breeding of D. melanogaster and other insects and provided new materials and a new approach for broadening the application of SPNE. 展开更多
关键词 SPNE drosophila melanogaster Growth and development Life cycle Breeding population
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黑腹果蝇Drosophila melanogaster因PM体系造成的卵巢败育的实验测定
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作者 王琼梅 《海南大学学报(自然科学版)》 CAS 1993年第4期29-32,共4页
黑腹果蝇在北美的群体自1950年后受到 P 转座子的侵入,现在该区域的果蝇几皆成为P 型,而我国境内的果蝇在 P 因子的特征上具有很大的差别,从我们22个品系的杂交实验,子代卵巢解剖的实验测定中可以得到明确的证明。杂交组合正反交结果不... 黑腹果蝇在北美的群体自1950年后受到 P 转座子的侵入,现在该区域的果蝇几皆成为P 型,而我国境内的果蝇在 P 因子的特征上具有很大的差别,从我们22个品系的杂交实验,子代卵巢解剖的实验测定中可以得到明确的证明。杂交组合正反交结果不同,证明与细胞质型有关。 展开更多
关键词 黑腹果蝇 PM体系 卵巢败育 果蝇
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Inhibition of foxo and minibrain in Dopaminergic Neurons Can Model Aspects of Parkinson Disease in Drosophila melanogaster
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作者 Mahin S. Chavoshi Brian E. Staveley 《Advances in Parkinson's Disease》 2016年第1期1-6,共6页
Symptoms of Parkinson Disease (PD), the second most common neurodegenerative disease, emerge due to degeneration of dopaminergic neurons. Recently, a genome wide study revealed a role for a foxo transcription factor i... Symptoms of Parkinson Disease (PD), the second most common neurodegenerative disease, emerge due to degeneration of dopaminergic neurons. Recently, a genome wide study revealed a role for a foxo transcription factor in PD. In the model organism Drosophila melanogaster, we have attempted 1) to inhibit the sole Drosophila homologue of foxo through the directed expression of a stable inducible RNAi transgene and 2) to indirectly increase foxo transcription activity through the inhibition of the kinase minibrain (mnb), a foxo transcriptional inhibitor. To evaluate the lifetime consequences upon the flies, longevity assays and locomotion over time assays were conducted. The inhibition of foxo by foxo-RNAi decreases life span significantly when expressed under the control of Tyrosine Hydroxylase-Gal4 (TH-Gal4). The targeted expression of mnb-RNAi, in the dopaminergic neurons, with an expected loss of suppression of foxo transcriptional activity, results in a significant loss of climbing ability. Thus alteration of foxo activity, both by RNA-inhibition and by down-regulation of an inhibitor of foxo, minibrain, produces novel Drosophila models of Parkinson Disease. 展开更多
关键词 drosophila melanogaster Model of Parkinson Disease FOXO minibrain
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Activation of Prophenol Oxidase PHOX-S with Limited Proteolysis in Drosophila Melanogaster
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作者 Nobuhiko Asada Taiki Hamada 《Journal of Life Sciences》 2010年第4期8-10,共3页
Prophenol oxidase isoform A1 was isolated from Drosophila melanogaster (The sequence has been deposited in GenBank data base under accession number AB557586) PHOX-S strain, and its characteristics and activation mec... Prophenol oxidase isoform A1 was isolated from Drosophila melanogaster (The sequence has been deposited in GenBank data base under accession number AB557586) PHOX-S strain, and its characteristics and activation mechanism were determined. The NH2-terminal region of PHOX-S A1 was determined to be comprised of 15 amino acids with the following sequence MTNMKMKMKAMMR. Comparison of an alignment in the known prophenol oxidase protein sequences from Drosophila melanogaster strains showed high homology in the copper-binding sequences at the Cu (A) site of the active center. Limited proteolysis takes place between Arg-50 and Val-51. Therefore, it is concluded that prophenol oxidase PHOX-S protein was evolved at the upstream, but no evolved at the central site in Drosophila melanogaster. 展开更多
关键词 ACTIVATION prophenol oxidase PHOX-S drosophila melanogaster.
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乙酸乙酯对果蝇(Drosophila melanogaster)的麻醉效应研究 被引量:5
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作者 徐静音 王子琪 +3 位作者 许雅丽 张一丁 任碧杰 杨大祥 《生物学杂志》 CAS CSCD 2011年第2期36-38,共3页
麻醉是果蝇实验中最基本的操作,乙醚是最常用的麻醉剂。但因为乙醚是二类易制毒化学品而被国家控制使用。报道一种容易获得的试剂——乙酸乙酯对果蝇的麻醉效果。实验采用的麻醉室大小为125cm3,每处理20~30只果蝇,乙酸乙酯剂量为40、80... 麻醉是果蝇实验中最基本的操作,乙醚是最常用的麻醉剂。但因为乙醚是二类易制毒化学品而被国家控制使用。报道一种容易获得的试剂——乙酸乙酯对果蝇的麻醉效果。实验采用的麻醉室大小为125cm3,每处理20~30只果蝇,乙酸乙酯剂量为40、80、120μL,以同等剂量的乙醚为对照,每个实验重复4次,用所有果蝇完全麻醉后20min及120min时的未苏醒率为指标评估麻醉效果及安全性。结果表明:乙酸乙酯对果蝇具有麻醉作用;麻醉时乙酸乙酯开始起效应的时间略晚于同等剂量的乙醚,但使果蝇完全麻醉的时间却比同等剂量的乙醚略短或相接近;麻醉持续的时间则长于同等剂量的乙醚。乙酸乙酯麻醉的果蝇,90%以上的果蝇均在120min内苏醒,表明在这些剂量范围内是安全的。乙酸乙酯完全可以替代乙醚用于果蝇的麻醉。 展开更多
关键词 乙酸乙酯 果蝇 麻醉
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