A method was established using hollow fiber-liquid phase microextraction（HF-LPME） followed by high performance liquid chromatography（HPLC） to determine the concentration of the free（unbound） drug in the solution...A method was established using hollow fiber-liquid phase microextraction（HF-LPME） followed by high performance liquid chromatography（HPLC） to determine the concentration of the free（unbound） drug in the solution of the drug and protein. Measurements of drug-protein binding ratios and free drug concentrations were then analyzed with the Klotz equation to determine the equilibrium binding constant and number of binding sites for drug-protein interaction. The optimized method allows one to perform the efficient extraction and separation of free drug from protein-bound drug, protein, and other interfering substances. This approach was used to characterize the binding of the anticholinergic drugs atropine sulfate and scopolamine hydrobromide to proteins in human plasma and bovine serum albumin（BSA）. The results demonstrate the utility of HF-LPME method for measuring free drug concentrations in protein-drug mixtures and determining the protein binding parameters of a pharmacologically important class of drugs.展开更多
To assess the relationship between serum levels of insulin-like growth factor-1 (IGF1)/IGF-binding protein-3 (IGFBP3) and the risk of esophageal carcinoma.METHODSWe assessed the relationship between the serum levels o...To assess the relationship between serum levels of insulin-like growth factor-1 (IGF1)/IGF-binding protein-3 (IGFBP3) and the risk of esophageal carcinoma.METHODSWe assessed the relationship between the serum levels of these molecules and the risk of esophageal cancer in a prospective, nested case-control study of participants from the Japan Collaborative Cohort Study. A baseline survey was conducted from 1988 to 1990. Of the 110585 enrolled participants, 35% donated blood samples. Those who had been diagnosed with esophageal cancer were considered cases for nested case-control studies. A conditional logistic model was used to estimate odds ratios for the incidence of esophageal cancer associated with serum IGF1 and IGFBP3 levels.RESULTSThirty-one cases and 86 controls were eligible for the present assessment. The molar ratio of IGF1/IGFBP3, which represents the free and active form of IGF1, was not correlated with the risk of esophageal carcinoma. A higher molar difference between IGFBP3 and IGF1, which estimates the free form of IGFBP3, was associated with a decreased risk of esophageal carcinoma (P = 0.0146), and people in the highest tertile had the lowest risk (OR = 0.107, 95%CI: 0.017-0.669). After adjustment for body mass index, tobacco use, and alcohol intake, the molar difference of IGFBP3-IGF1 was inversely correlated with the risk of esophageal carcinoma (P = 0.0150).CONCLUSIONThe free form of IGFBP3, which is estimated by this molar difference, may be inversely associated with esophageal cancer incidence.展开更多
A bound state formalism derived from a fermion-boson symmetric Lagrangian has been used to calculate the nucleon masses, the charge neutrality of the neutron, the magnetic moments and the electromagnetic form factor r...A bound state formalism derived from a fermion-boson symmetric Lagrangian has been used to calculate the nucleon masses, the charge neutrality of the neutron, the magnetic moments and the electromagnetic form factor ratios μpGEp/GMpand μnGEn/GMn. A quantitative description is obtained, assuming a mixing of a scalar bound state of 3(f f¯)fstructure with its corresponding vector (f f¯)fstate (f indicating massless elementary fermions). Only a few parameters are needed, mainly fixed by energy and momentum conservation. The nucleon stability is explained by an extra binding in the confinement potential, negative for electric and positive for magnetic binding of the proton, and opposite for the neutron. The stronger electric extra binding of the proton allows a decay of the neutron to proton and electron.展开更多
目的探讨肝素结合蛋白(heparin binding protein,HBP)、中性粒细胞与淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)、淋巴细胞与单核细胞比值(lymphocyte to monocyte ratio,LMR)及血小板与淋巴细胞比值(platelet to lymphocyte ra...目的探讨肝素结合蛋白(heparin binding protein,HBP)、中性粒细胞与淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)、淋巴细胞与单核细胞比值(lymphocyte to monocyte ratio,LMR)及血小板与淋巴细胞比值(platelet to lymphocyte ratio,PLR)在人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者/获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS)患者免疫功能重建评估中的应用价值。方法选取浙江中医药大学附属杭州西溪医院2023年1月1日至6月30日门诊HIV/AIDS患者138例,根据抗反转录病毒治疗后患者CD4^(+)T淋巴细胞计数分为免疫功能重建良好组(n=108)和免疫功能重建不良组(n=30),比较两组患者HBP、NLR、LMR及PLR水平,采用受试者操作特征(receiver operating characteristic,ROC)曲线评估HBP、NLR、LMR及PLR在患者免疫功能重建中的应用价值。结果免疫功能重建良好组患者的NLR、PLR明显高于免疫功能重建不良组(P<0.05),免疫功能重建良好组患者的LMR明显低于免疫功能重建不良组(P<0.05),而HBP在两组间差异无统计学意义(P>0.05)。LMR对评估HIV/AIDS患者免疫功能重建是否良好的效能最佳,曲线下面积(area under the curve,AUC)为0.803,PLR次佳(AUC=0.796),NLR较差(AUC=0.728)。结论NLR、LMR及PLR与HIV/AIDS患者的免疫功能紧密相关,可作为评估HIV/AIDS患者免疫功能重建是否良好的检测指标。展开更多
基金Supported by the National Natural Science Foundation of China(No.81041084)the Natural Science Foundation of Shanxi Province, China(No.2007011086)the Undergraduate Innovation Fund of Taiyuan City, China(No.08122034)
文摘A method was established using hollow fiber-liquid phase microextraction（HF-LPME） followed by high performance liquid chromatography（HPLC） to determine the concentration of the free（unbound） drug in the solution of the drug and protein. Measurements of drug-protein binding ratios and free drug concentrations were then analyzed with the Klotz equation to determine the equilibrium binding constant and number of binding sites for drug-protein interaction. The optimized method allows one to perform the efficient extraction and separation of free drug from protein-bound drug, protein, and other interfering substances. This approach was used to characterize the binding of the anticholinergic drugs atropine sulfate and scopolamine hydrobromide to proteins in human plasma and bovine serum albumin（BSA）. The results demonstrate the utility of HF-LPME method for measuring free drug concentrations in protein-drug mixtures and determining the protein binding parameters of a pharmacologically important class of drugs.
基金Supported by the Ministry of Education,Culture,Sports,Science,and Technology and from the Ministry of Health,Labour and Welfare,Japan
文摘To assess the relationship between serum levels of insulin-like growth factor-1 (IGF1)/IGF-binding protein-3 (IGFBP3) and the risk of esophageal carcinoma.METHODSWe assessed the relationship between the serum levels of these molecules and the risk of esophageal cancer in a prospective, nested case-control study of participants from the Japan Collaborative Cohort Study. A baseline survey was conducted from 1988 to 1990. Of the 110585 enrolled participants, 35% donated blood samples. Those who had been diagnosed with esophageal cancer were considered cases for nested case-control studies. A conditional logistic model was used to estimate odds ratios for the incidence of esophageal cancer associated with serum IGF1 and IGFBP3 levels.RESULTSThirty-one cases and 86 controls were eligible for the present assessment. The molar ratio of IGF1/IGFBP3, which represents the free and active form of IGF1, was not correlated with the risk of esophageal carcinoma. A higher molar difference between IGFBP3 and IGF1, which estimates the free form of IGFBP3, was associated with a decreased risk of esophageal carcinoma (P = 0.0146), and people in the highest tertile had the lowest risk (OR = 0.107, 95%CI: 0.017-0.669). After adjustment for body mass index, tobacco use, and alcohol intake, the molar difference of IGFBP3-IGF1 was inversely correlated with the risk of esophageal carcinoma (P = 0.0150).CONCLUSIONThe free form of IGFBP3, which is estimated by this molar difference, may be inversely associated with esophageal cancer incidence.
文摘A bound state formalism derived from a fermion-boson symmetric Lagrangian has been used to calculate the nucleon masses, the charge neutrality of the neutron, the magnetic moments and the electromagnetic form factor ratios μpGEp/GMpand μnGEn/GMn. A quantitative description is obtained, assuming a mixing of a scalar bound state of 3(f f¯)fstructure with its corresponding vector (f f¯)fstate (f indicating massless elementary fermions). Only a few parameters are needed, mainly fixed by energy and momentum conservation. The nucleon stability is explained by an extra binding in the confinement potential, negative for electric and positive for magnetic binding of the proton, and opposite for the neutron. The stronger electric extra binding of the proton allows a decay of the neutron to proton and electron.
文摘目的探讨肝素结合蛋白(heparin binding protein,HBP)、中性粒细胞与淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)、淋巴细胞与单核细胞比值(lymphocyte to monocyte ratio,LMR)及血小板与淋巴细胞比值(platelet to lymphocyte ratio,PLR)在人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染者/获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS)患者免疫功能重建评估中的应用价值。方法选取浙江中医药大学附属杭州西溪医院2023年1月1日至6月30日门诊HIV/AIDS患者138例,根据抗反转录病毒治疗后患者CD4^(+)T淋巴细胞计数分为免疫功能重建良好组(n=108)和免疫功能重建不良组(n=30),比较两组患者HBP、NLR、LMR及PLR水平,采用受试者操作特征(receiver operating characteristic,ROC)曲线评估HBP、NLR、LMR及PLR在患者免疫功能重建中的应用价值。结果免疫功能重建良好组患者的NLR、PLR明显高于免疫功能重建不良组(P<0.05),免疫功能重建良好组患者的LMR明显低于免疫功能重建不良组(P<0.05),而HBP在两组间差异无统计学意义(P>0.05)。LMR对评估HIV/AIDS患者免疫功能重建是否良好的效能最佳,曲线下面积(area under the curve,AUC)为0.803,PLR次佳(AUC=0.796),NLR较差(AUC=0.728)。结论NLR、LMR及PLR与HIV/AIDS患者的免疫功能紧密相关,可作为评估HIV/AIDS患者免疫功能重建是否良好的检测指标。
