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Polyphenols Extracted from Enteromorpha clathrata Induce Apoptosis in Hepa1-6 Cell by Activating the Mitochondrial Apoptosis Signaling Pathways
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作者 ZHANG Panxue SUN Bolun +4 位作者 TONG Jingjing XIA Geran ZHANG Jinjie LI Chao YANG Wenge 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第5期1393-1402,共10页
Green alga Enteromorpha clathrata(E.clathrata)contains a variety of bioactive compounds,including polysaccharides,polyphenols and fat-soluble pigments etc.,among which polyphenols exhibit a wide range of medicinal pro... Green alga Enteromorpha clathrata(E.clathrata)contains a variety of bioactive compounds,including polysaccharides,polyphenols and fat-soluble pigments etc.,among which polyphenols exhibit a wide range of medicinal properties.E.clathrata polyphenols(ECPs)have shown various biological activities such as antioxidant,anti-inflammatory and antidiabetic effects;however,the potential of ECPs as an anti-cancer reagent remains unclear.The aim of this study was to investigate the anti-tumor activity and underlying mechanisms of ECPs on hepatocellular carcinoma.The cytotoxicity of Hepa1-6 cells was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and lactate dehydrogenase(LDH)assay.Flow cytometry and fluorescence microscope analysis of cell apoptosis after annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI)staining.2',7'-dichlorodihydrofluorescein diacetate(DCFH-DA)assay was used for intracellular reactive oxygen species(ROS)detection.caspase-9 activity was determined using cspase-9 colorimetric assay.Mitochondrial transmembrane potential(Δψm)was measured using JC-1.Western blot and quantitative real-time PCR(qPCR)were used to assess the expressions of the apoptosis regulators Bax,Bcl-2,cytochrome c and caspase-3.It was found that ECPs showed a dose-dependent cytotoxicity against Hepa1-6 cells by inducing apoptosis.The apoptosis in ECPs-treated Hepa1-6 cells was accompanied by the loss of mitochondrial membrane potential,elevated ROS generation,increased release of mitochondrial cytochrome c,and up-regulation of caspase-9 and caspase-3.The expressions of Bax(pro-apoptotic molecule)and Bcl-2(apoptosis suppressor)were up-regulated and down-regulated,respectively,at both mRNA and protein levels.These molecular alterations revealed that ECPs caused apoptosis of cells through the mitochondrial pathway,suggesting that ECPs are potential candidates to be developed for liver cancer treatment. 展开更多
关键词 enteromorpha clathrate POLYPHENOL anti-cancer activity apoptosis mitochondrial-dependent pathway
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Effects of Polysaccharides from Enteromorpha prolifera on Hepatorenal Syndrome in Mice
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作者 Shanshan WANG Jiayan ZHOU +7 位作者 Yue HE Yating ZHOU Qiuxi YANG Qianqian YUAN Qingying DU Hanchi CHENG Suduo JIANG Xiaojuan ZHAO 《Medicinal Plant》 CAS 2023年第1期50-54,共5页
[Objectives]This study was conducted to evaluate the protective effect of polysaccharides from Enteromorpha prolifera(PEP)on mice with hepatorenal syndrome induced by carbon tetrachloride.[Methods]A mouse hepatorenal ... [Objectives]This study was conducted to evaluate the protective effect of polysaccharides from Enteromorpha prolifera(PEP)on mice with hepatorenal syndrome induced by carbon tetrachloride.[Methods]A mouse hepatorenal syndrome model was induced by carbon tetrachloride.The serum levels of lipid,total antioxidant capacity,liver and kidney function,pathological changes of liver and kidney were selected to clarity the effectiveness of PEP on hepatorenal syndrome in mice.[Results]PEP effectively lowered the serum levels of lipid,increased total antioxidant capacity,improved liver and kidney injury,and alleviated pathological changes of liver and kidney of mice induced by carbon tetrachloride.[Conclusions]PEP has a potent preventive effect on hepatorenal syndrome induced by carbon tetrachloride in mice,which provides theoretical support for future clinical application of PEP. 展开更多
关键词 Polysaccharides from enteromorpha prolifera Carbon tetrachloride Hepatorenal syndrome
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石莼属(Ulva)和浒苔属(Enteromorpha)绿藻的RAPD分析 被引量:19
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作者 杨君 安利佳 +3 位作者 王茜 王宏伟 苏乔 康晓慧 《海洋与湖沼》 CAS CSCD 北大核心 2000年第4期408-413,共6页
于 1 997年下半年在大连石庙海区采集石莼、孔石莼、砺菜、缘管浒苔、肠浒苔、管浒苔、刚毛藻等 7种海藻样品 ,采用RAPD技术对大连地区绿藻门石莼属和浒苔属进行了初步的分子系统学研究。所试 60个引物中有 32个经扩增得到了 2 37个多... 于 1 997年下半年在大连石庙海区采集石莼、孔石莼、砺菜、缘管浒苔、肠浒苔、管浒苔、刚毛藻等 7种海藻样品 ,采用RAPD技术对大连地区绿藻门石莼属和浒苔属进行了初步的分子系统学研究。所试 60个引物中有 32个经扩增得到了 2 37个多态片段。应用PHYLIP软件包 ,按照UPGMA法和N -J法聚类分析的结果均表明 ,缘管浒苔 (Enteromorphalinza)与石莼属有较近的亲缘关系 ,应深入探究其分类地位。 展开更多
关键词 石莼属 浒苔属 RAPD 绿藻
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浒苔(Enteromorpha prolifera)生长及孢子释放的生态因子研究 被引量:93
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作者 王建伟 阎斌伦 +2 位作者 林阿朋 胡静平 沈颂东 《海洋通报》 CAS CSCD 北大核心 2007年第2期60-65,共6页
通过考察浒苔属浒苔藻体生长及孢子释放的生态因子条件,确定其各单因子的适应范围及最适范围。结果表明:浒苔藻体生长对盐度、温度、光照强度和酸碱度的适应范围分别为16~40、10℃~30℃、>9μmol/m2s、6~10,最适范围分别为24~28... 通过考察浒苔属浒苔藻体生长及孢子释放的生态因子条件,确定其各单因子的适应范围及最适范围。结果表明:浒苔藻体生长对盐度、温度、光照强度和酸碱度的适应范围分别为16~40、10℃~30℃、>9μmol/m2s、6~10,最适范围分别为24~28、20℃~25℃、>18μmol/m2s、8~9,在盐度、温度、光照强度和酸碱度分别为24、25℃、72μmol/m2s、8的条件下达到最大生长量;浒苔释放孢子对盐度、温度、光照强度和酸碱度的适应范围分别为12~40、15℃~35℃、>9μmol/m2s、6~10,最适范围分别为28~40、20℃~35℃、>36μmol/m2s、8~9,在盐度、温度、光照强度和酸碱度分别为32、35℃、144μmol/m2s、9的条件下达到最大释放量。浒苔藻体生长及孢子释放的各单因子适应范围基本相同,最适范围区别明显。 展开更多
关键词 浒苔 生态因子 生长量 孢子释放
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黄海海域浒苔属(Enteromorpha)生态特征初探 被引量:23
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作者 忻丁豪 任松 +4 位作者 何培民 刘富平 张丽旭 徐韧 程祥圣 《海洋环境科学》 CAS CSCD 北大核心 2009年第2期190-192,共3页
通过对2008年6月在黄海爆发的大型绿藻(浒苔属)进行实验室培养试验,对其生态特征有了初步了解。结果表明,该藻属广温、广盐性的海洋绿藻,对环境的适应能力和繁殖能力较强。在培养温度范围15~32℃,盐度范围5—29之间都能存活,其... 通过对2008年6月在黄海爆发的大型绿藻(浒苔属)进行实验室培养试验,对其生态特征有了初步了解。结果表明,该藻属广温、广盐性的海洋绿藻,对环境的适应能力和繁殖能力较强。在培养温度范围15~32℃,盐度范围5—29之间都能存活,其中水温为23℃,盐度为25和29时,其生长较好。在富营养水体环境中,其生长需要的消耗水中大量的N、P营养盐,并且在当N:P为10:1时生长率达到最大。 展开更多
关键词 浒苔属 N:P 生长率
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黄、渤海漂移浒苔(Enteromorpha prolifera)脂肪酸组成及聚类分析的研究 被引量:12
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作者 杨佰娟 郑立 +3 位作者 陈军辉 臧家业 王小如 黎先春 《海洋与湖沼》 CAS CSCD 北大核心 2009年第5期627-632,共6页
于2008年7月在黄、渤海区域采集了17个漂移浒苔样品,结合3个本地种,对其脂肪酸组成进行了GC-MS分析。结果表明,所有浒苔中优势脂肪酸为十六碳酸,相对含量在27.82%—43.27%之间。藻体中还含有丰富的PUFA,主要为9,12,15-十八碳三烯酸、6,9... 于2008年7月在黄、渤海区域采集了17个漂移浒苔样品,结合3个本地种,对其脂肪酸组成进行了GC-MS分析。结果表明,所有浒苔中优势脂肪酸为十六碳酸,相对含量在27.82%—43.27%之间。藻体中还含有丰富的PUFA,主要为9,12,15-十八碳三烯酸、6,9,12-十八碳三烯酸、9,12-十八碳二烯酸、8,11,14-二十碳三烯酸,另外还含有EPA不饱和脂肪酸。以欧式距离平方为距离测量技术,类间距用平均链锁法,对20个不同地理位置的浒苔样品进行聚类分析,结果分为4大类,所有漂浮浒苔聚为第一类,青岛和连云港的本地种分别聚为另三类,说明青岛近海漂浮的浒苔不是青岛本地生浒苔,而是从外海漂移过来的,第一类的聚类结果显示了浒苔的漂移路径。结果表明,藻类脂肪酸的系统聚类分析为海洋绿藻分类与量化评价提供了一种好方法。 展开更多
关键词 浒苔 脂肪酸 聚类分析
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浒苔(Enteromorpha prolifera)生活史的初步研究 被引量:94
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作者 王晓坤 马家海 +1 位作者 叶道才 陈孝德 《海洋通报》 CAS CSCD 北大核心 2007年第5期112-116,共5页
通过实验室培养实验,全面地观察描述了浒苔生活史各个阶段的发育情况。雌雄异体的配子体成熟后,放散双鞭毛的配子,雌雄配子结合成合子进而发育成孢子体,配子也可以进行单性生殖发育成膜状的配子体。孢子体成熟放散四鞭毛的游孢子,游孢... 通过实验室培养实验,全面地观察描述了浒苔生活史各个阶段的发育情况。雌雄异体的配子体成熟后,放散双鞭毛的配子,雌雄配子结合成合子进而发育成孢子体,配子也可以进行单性生殖发育成膜状的配子体。孢子体成熟放散四鞭毛的游孢子,游孢子固着后,可直接发育成配子体。其生活史是单倍体的配子体与二倍体的孢子体相互交替的同形世代交替。雌雄配子具有正趋光性,易大量聚集,结合后同游孢子一样都呈负趋光性。 展开更多
关键词 绿藻 浒苔 生活史 异配生殖 配子 游孢子
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浒苔Enteromorpha prolifera组织培养初步研究 被引量:13
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作者 林阿朋 王建伟 +1 位作者 阎斌伦 沈颂东 《生态科学》 CSCD 2006年第4期320-324,共5页
通过将大型绿藻——浒苔切成一定大小的藻体片段,在实验室条件下用液体和固体平板组织培养的方法,系统地研究了在藻体切段条件下,浒苔组织块中细胞的发育路径和趋势。在培养的过程中发现叶状体中细胞的发育能力和方向都具有明显的极性,... 通过将大型绿藻——浒苔切成一定大小的藻体片段,在实验室条件下用液体和固体平板组织培养的方法,系统地研究了在藻体切段条件下,浒苔组织块中细胞的发育路径和趋势。在培养的过程中发现叶状体中细胞的发育能力和方向都具有明显的极性,且浒苔组织块中细胞的发育速度和规模与组织块大小和其组织块中的位置有关。在切段培养的条件下,浒苔细胞有5种发育途径:1.分化为孢子囊,2.细胞极化下端延伸形成类似于假根的结构,3.分裂成细胞团并在后续的发育过程中成苗,4.细胞进一步极化形成带假根的单细胞苗,5.维持一定状态不再分化。而切段培养条件下,藻段中的不同细胞可能发生不同的发育情况。 展开更多
关键词 绿藻 浒苔 组织培养 发育
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浒苔(Enteromorpha prolifera)藻体发育的显微观察 被引量:12
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作者 王建伟 林阿朋 +2 位作者 李艳燕 沈颂东 阎斌伦 《生态科学》 CSCD 2006年第5期400-404,共5页
通过显微镜观察以及石蜡切片法观察了浒苔属浒苔藻体的发育过程,阐述了浒苔从孢子释放到形成成熟藻体这一发育过程的显微特征。结果表明:浒苔孢子由成熟体细胞分化发育而成,每个成熟体细胞可形成10~30个孢子;孢子首先分裂为2细胞结构,... 通过显微镜观察以及石蜡切片法观察了浒苔属浒苔藻体的发育过程,阐述了浒苔从孢子释放到形成成熟藻体这一发育过程的显微特征。结果表明:浒苔孢子由成熟体细胞分化发育而成,每个成熟体细胞可形成10~30个孢子;孢子首先分裂为2细胞结构,2细胞具有明显的极性,基细胞发育成为假根,顶端细胞不断进行横分裂形成丝状体;丝状体顶端以下细胞通过纵分裂形成管状叶状体,顶端细胞始终保持横分裂;纵分裂分为切向分裂与径向分裂,切向分裂增加管状叶状体管周细胞数从而使管体增大,径向分裂形成管外壁突起细胞,最终发育为分枝;管状叶状体管腔内部有绒毛状的突起及糖蛋白成分的网架结构;当管状叶状体管周细胞达到30~50h,管腔内部突起消失,网架结构收缩拉紧,管壁细胞贴近形成片状叶状体;整个浒苔藻体始终保持极性发育。 展开更多
关键词 浒苔 藻体发育 石蜡切片 显微观察
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高浓度CO_2对条浒苔(Enteromorpha clathrata)生长和一些生理生化特征的影响 被引量:13
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作者 邹定辉 陈雄文 《海洋通报》 CAS CSCD 北大核心 2002年第5期38-45,共8页
本试验利用 CO2 培养箱,通过不同 CO2 浓度的空气通气培 养,研 究 高 浓 度 CO2 对 条 浒 苔 (Enteromorpha clathrata) 生长、光合作用及有关生理生化特征的影响。高浓度 CO2 使得海水中的溶解性无机碳浓度增加,而 pH 值下降。700μ... 本试验利用 CO2 培养箱,通过不同 CO2 浓度的空气通气培 养,研 究 高 浓 度 CO2 对 条 浒 苔 (Enteromorpha clathrata) 生长、光合作用及有关生理生化特征的影响。高浓度 CO2 使得海水中的溶解性无机碳浓度增加,而 pH 值下降。700μL/L 和 5 000μL/L 的 CO2 浓度培养条件使条浒苔的相对生长速率分别提高了 12 % 和 23 %,而光合能力均下降了 25 %。 5 000μL/L 的 CO2 浓度使得条浒苔光合作用光补偿点及无机碳补偿点提高;同时,条浒苔的叶绿素含量及可溶性蛋白含量下降,而可溶性碳水化合物含量增加;另外,条浒苔对 NO3- 的吸收速度增加,而硝酸还原酶活性则下降。700μL/L 的 CO2 浓度比 5 000μL/L 的 CO2 浓度对这些指标的影响较小。 展开更多
关键词 条浒苔 CO2 光合作用 生理生化 二氧化碳 叶绿素 绿藻
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Molecular phylogenetic analysis of attached Ulvaceae species and free-floating Enteromorpha from Qingdao coasts in 2007 被引量:30
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作者 姜鹏 王金锋 +3 位作者 崔玉琳 李友训 林瀚智 秦松 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2008年第3期276-279,共4页
Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,inclu... Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. 展开更多
关键词 free-floating enteromorpha ULVA ITS RDNA Qingdao
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湛江海域浒苔属Enteromorpha种类的形态与显微结构 被引量:4
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作者 赵素芬 刘丽丝 +2 位作者 孙会强 黄小玲 何山 《广东海洋大学学报》 CAS 2013年第6期1-8,共8页
用比较解剖学方法观察湛江沿海浒苔属(Enteromorpha)海藻的形态结构,并结合统计分析对其进行物种鉴定。结果表明,依据藻体分枝的有无、数量及分枝与主枝直径的大小等形态特征,鉴定的浒苔属海藻有6种1个变型,分别为缘管浒苔(E.linza)、... 用比较解剖学方法观察湛江沿海浒苔属(Enteromorpha)海藻的形态结构,并结合统计分析对其进行物种鉴定。结果表明,依据藻体分枝的有无、数量及分枝与主枝直径的大小等形态特征,鉴定的浒苔属海藻有6种1个变型,分别为缘管浒苔(E.linza)、肠浒苔(E.intestinalis)、扁浒苔(E.compressa)、浒苔(E.prolifera)、条浒苔(E.clathrata)、曲浒苔(E.flexuosa)和肠浒苔宽叶变型(E.intestinalis f.broadifolium)。藻体长度由大到小依次为浒苔、条浒苔、缘管浒苔、肠浒苔、扁浒苔和肠浒苔宽叶变型;细胞呈多角形,藻体的位置不同其细胞大小有所变化,除曲浒苔和条浒苔外,其他4种1个变型浒苔的中部细胞最小;所有种类的细胞均有淀粉核,其中肠浒苔、缘管浒苔和扁浒苔仅1个淀粉核,而其他种出现多个淀粉核,尤其条浒苔和曲浒苔数量最多,可达6个以上。定量描述浒苔属6种1个变型的形态特征、内部结构,并发现浒苔属海藻非中空管状的结构特征。 展开更多
关键词 浒苔属 形态 结构 湛江海域
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浒苔(Enteromorpha prolifera)的分类鉴定、生境习性及分布 被引量:61
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作者 丁兰平 栾日孝 《海洋与湖沼》 CAS CSCD 北大核心 2009年第1期68-71,共4页
结合过去的工作基础,对2008年5月底以来黄海西部沿海水域漂浮聚集的绿藻样品进行了分类学研究。经鉴定,其为绿藻门石莼目石莼科浒苔属的浒苔Enteromorpha prolifera,其主要分类鉴定特征为藻体暗绿色或亮绿色、管状扁压、中空、主枝明显... 结合过去的工作基础,对2008年5月底以来黄海西部沿海水域漂浮聚集的绿藻样品进行了分类学研究。经鉴定,其为绿藻门石莼目石莼科浒苔属的浒苔Enteromorpha prolifera,其主要分类鉴定特征为藻体暗绿色或亮绿色、管状扁压、中空、主枝明显、分枝较多、密集且细长。文中对其生境习性及其分布特征也作了较详细地介绍。 展开更多
关键词 浒苔 分类 生境习性 分布 黄海西部
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不同地区浒苔(Enteromorpha prolifera)群体遗传多样性的ISSR分析 被引量:7
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作者 韩晓磊 徐建荣 +5 位作者 汪洁华 张涛 杨立恩 穆新武 姚春燕 许璞 《常熟理工学院学报》 2009年第10期57-60,共4页
通过ISSR分子标记技术对6个不同地区浒苔(Enteromorpha prolifera)群体(浙江鳌江、浙江宁波、广东南澳、江苏南通、福建福鼎、浙江南麂岛)进行了遗传多样性分析.结果显示,从100条ISSR引物中筛选出5条引物,共得到36个清晰的扩增位点,其... 通过ISSR分子标记技术对6个不同地区浒苔(Enteromorpha prolifera)群体(浙江鳌江、浙江宁波、广东南澳、江苏南通、福建福鼎、浙江南麂岛)进行了遗传多样性分析.结果显示,从100条ISSR引物中筛选出5条引物,共得到36个清晰的扩增位点,其中多态性位点10个;6个地区浒苔的多态位点比率为27.78%,平均杂合度为0.1256,Shannon's信息指数为0.1795;群体间的遗传距离在0.0870-0.2062之间,平均遗传距离为0.1654.研究表明,6个地区浒苔的遗传多样性处于较低水平,遗传变异相对贫乏;它们之间的亲缘关系与其地理距离的远近没有直接的关系,并揭示6个地区的浒苔是属于同一物种. 展开更多
关键词 浒苔 ISSR 遗传多样性 亲缘关系
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The possibility analysis of habitats,origin and reappearance of bloom green alga(Enteromorpha prolifera) on inshore of western Yellow Sea 被引量:12
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作者 丁兰平 费修绠 +2 位作者 陆勤勤 邓蕴彦 连绍兴 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2009年第3期421-424,共4页
Combining some information from field investigation of algae along the coastal areas in China and a few pictures materialized from the western Yellow Sea in 2008,authors analyze the necessary conditions and possible w... Combining some information from field investigation of algae along the coastal areas in China and a few pictures materialized from the western Yellow Sea in 2008,authors analyze the necessary conditions and possible water area in China producing a large biomass,some reasons for firestorm,and the possibility of the reappearance of marine bloom green alga Enteromorpha prolifera.The change of habitats and the increase of nutritional levels related to the water area could be considered as direct reasons.It was transferred northward by the combination of the flow of rainwater,wind and alongshore marine current.The original region of large biomass produced is possibly located in the southwestern Yellow sea.It will possibly be appearing again in the coming years or in the future.A summary is also given referring to its reproduction,development and distribution worldwide. 展开更多
关键词 bloom green alga enteromorpha prolifera ORIGIN HABITATS reappearance western Yellow Sea
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Acute toxicity of live and decomposing green alga Ulva (Enteromorpha) prolifera to abalone Haliotis discus hannai 被引量:11
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作者 王超 于仁成 周名江 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2011年第3期541-546,共6页
From 2007 to 2009, large-scale blooms of green algae (the so-called "green tides") occurred every summer in the Yellow Sea, China. In June 2008, huge amounts of floating green algae accumulated along the coa... From 2007 to 2009, large-scale blooms of green algae (the so-called "green tides") occurred every summer in the Yellow Sea, China. In June 2008, huge amounts of floating green algae accumulated along the coast of Qingdao and led to mass mortality of cultured abalone and sea cucumber. However, the mechanism for the mass mortality of cultured animals remains undetermined. This study examined the toxic effects of Ulva (Enteromorpha) prolifera, the causative species of green tides in the Yellow Sea during the last three years. The acute toxicity of fresh culture medium and decomposing algal effluent of U. prolifera to the cultured abalone Haliotis discus hannai were tested. It was found that both fresh culture medium and decomposing algal effluent had toxic effects to abalone, and decomposing algal effluent was more toxic than fresh culture medium. The acute toxicity of decomposing algal effluent could be attributed to the ammonia and sulfide presented in the effluent, as well as the hypoxia caused by the decomposition process. 展开更多
关键词 green tide macroalgal bloom Ulva enteromorpha)prolifera acute toxicity ABALONE Haliotis discus hannai
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Selenylation Modification of Degraded Polysaccharide from Enteromorpha prolifera and Its Biological Activities 被引量:4
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作者 LV Haitao DUAN Ke SHAN Hu 《Journal of Ocean University of China》 SCIE CAS CSCD 2018年第2期445-450,共6页
Polysaccharide extracted from Enteromorpha prolifera possessed excellent biological activities,but its molecular weight was greatly high which influenced the activity.Organic Se had higher biological activities and wa... Polysaccharide extracted from Enteromorpha prolifera possessed excellent biological activities,but its molecular weight was greatly high which influenced the activity.Organic Se had higher biological activities and was safer than inorganic Se species.In the present study,Enteromorpha polysaccharide was degraded to low molecular weight by free-radical degradation method of H_2O_2 and ascorbic acid.By single factor and orthogonal experiments,the optimal degradation conditions were reaction time of 2 h,reaction temperature of 50℃,H_2O_2/ascorbic acid(n/n=1:1)concentration of 15 mmol L^(-1),and solid-liquid ratio of 1:50(g mL^(-1)).Then,the degraded polysaccharide was chemically modified to obtain its selenide derivatives by nitric acid-sodium selenite method.The selenium content was 1137.29μg g^(-1),while the content of sulfate radical had no change.IR spectra indicated that the selenite ester group was formed.Degraded polysaccharide selenide was characterized and evaluated for antioxidant,antifungal and antibacterial activities.The results showed that degraded polysaccharide selenide had strong capacity of scavenging DPPH and·OH free radical.It had significant antibacterial properties for Escherichia coli,Bacillus subtilis and Salmonella spp.,and it also had significant antifungal properties for Apple anthrax.The result ascertained degradation and selenylation modification did not change the main structure of polysaccharides.It was possible that free-radical degradation was an effective way for enhancing antioxidant activity to decrease molecular weight of polysaccharides. 展开更多
关键词 enteromorpha PROLIFERA POLYSACCHARIDE FREE-RADICAL degradation selenylation MODIFICATION BIOLOGICAL activity
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Effect of temperature and irradiance on the growth and reproduction of Enteromorpha prolifera J.Ag.(Chlorophycophyta,Chlorophyceae) 被引量:8
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作者 付刚 姚建亭 +7 位作者 刘福利 刘吉东 王秀良 付万冬 李大鹏 周名江 孙松 段德麟 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2008年第4期357-362,共6页
Effect of temperature and irradiance on growth and reproduction of Enteromorpha prolifera that bloomed offshore along the Qingdao coast in summer 2008, was studied. It was showed that E. prolifera propagated mainly as... Effect of temperature and irradiance on growth and reproduction of Enteromorpha prolifera that bloomed offshore along the Qingdao coast in summer 2008, was studied. It was showed that E. prolifera propagated mainly asexually with specific growth rate (SGR) of 10.47 at 25℃/40 μmol m^-2s^-1. Under this condition, gametes with two flagellate formed and released in 5 days. At the beginning of the development, the unicell gamete divided into two cells with heteropolarity, and then the apical cell developed into thalli primordial cells, whereas the basal cell developed into rhizoid primordial cells. In 8-day culture, the monoplast gamete developed into juvenile germling of 240 μm in length. Unreleased gametes can develop directly within the alga body. E. prolifera could either reproduce through lateral branching or fragmenting except apomixis revealed by Microscopic observation. On aged tissue of E. prolifera, although the degraded pigments partially remained in faded algal filaments, numerous vegetative cells could still divide actively in the algal tissues. 展开更多
关键词 enteromorpha prolifera growth REPRODUCTION gamete development
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Effect of polysaccharide from Enteromorpha prolifera on maize seedlings under NaCl stress 被引量:3
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作者 LIU Song LI Bing +2 位作者 CHEN Xiaolin QIN Yukun LI Pengcheng 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2019年第4期1372-1381,共10页
In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We ... In this study, a polysaccharide from Enteromorpha prolifera (EP) was extracted and its effect on maize seedlings under NaCl stress was investigated. Firstly, the components and structure of the EP were determined. We found that EP is a sulfated polysaccharide of high-molecular weight (Mw, 1 840 KDa) heteropolysaccharides and the main monosaccharide is rhamnose. The polysaccharide was applied to explore its effect on the growth of maize seedlings and its defense response under a salt stress. The results show that EP could promote the growth of maize seedlings under the salt stress. In addition, EP was shown able to significantly regulate membrane permeability and adjustment of osmotic substances such as soluble protein, soluble sugar, and proline, antioxidant enzymes containing superoxide dismutase, catalase, peroxidase, and ascorbate peroxidase. Therefore, EP is an effective salt-resistant substance for the growth of maize seedlings under NaCl stress. 展开更多
关键词 POLYSACCHARIDES enteromorpha PROLIFERA MAIZE SEEDLING NaCl stress
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Dietary Enteromorpha polysaccharide-Zn supplementation regulates amino acid and fatty acid metabolism by improving the antioxidant activity in chicken 被引量:5
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作者 Teketay Wassie Xinyi Duan +2 位作者 Chunyan Xie Ruxia Wang Xin Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2022年第3期878-896,共19页
Background:Enteromorpha prolifera(E.prolifera)polysaccharide has become a promising feed additive with a variety of physiological activities,such as anti-oxidant,anti-cancer,anti-diabetic,immunomodulatory,hypolipidemi... Background:Enteromorpha prolifera(E.prolifera)polysaccharide has become a promising feed additive with a variety of physiological activities,such as anti-oxidant,anti-cancer,anti-diabetic,immunomodulatory,hypolipidemic,and cation chelating ability.However,whether Enteromorpha polysaccharide-trace element complex supplementation regulates amino acid and fatty acid metabolism in chicken is largely unknown.This study was conducted to investigate the effects of E.prolifera polysaccharide(EP)-Zn supplementation on growth performance,amino acid,and fatty acid metabolism in chicken.Methods:A total of 184 one-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with 8 replicates,12 chickens per replicate,and fed either the basal diet(control group)or basal diet plus E.prolifera polysaccharide-Zinc(400 mg EP-Zn/kg diet).Results:Dietary EP-Zn supplementation significantly increased(P<0.05)the body weight,average daily gain,muscle antioxidant activity,serum HDL level,and reduced serum TG and LDL concentration.In addition,dietary EPZn supplementation could modulate ileal amino acid digestibility and upregulate the mRNA expression of amino acid transporter genes in the jejunum,ileum,breast muscle,and liver tissues(P<0.05).Compared with the control group,breast meat from chickens fed EP-Zn had higher(P<0.05)Pro and Asp content,and lower(P<0.05)Val,Phe,Gly,and Cys free amino acid content.Furthermore,EP-Zn supplementation upregulated(P<0.05)the mRNA expressions of mTOR and anti-oxidant related genes,while down-regulated protein degradation related genes in the breast muscle.Breast meat from EP-Zn supplemented group had significantly lower(P<0.05)proportions ofΣn-3 PUFA,and a higher percentage ofΣn-6 PUFA and the ratio of n-6/n-3 PUFA.Besides,EP-Zn supplementation regulated lipid metabolism by inhibiting the gene expression of key enzymes involved in the fatty acid synthesis and activating genes that participated in fatty acid oxidation in the liver tissue.Conclusions:It is concluded that EP-Zn complex supplementation regulates apparent ileal amino acid digestibility,enhances amino acid metabolism,and decreases oxidative stress-associated protein breakdown,thereby improving the growth performance.Furthermore,it promotes fatty acid oxidation and restrains fat synthesis through modulating lipid metabolism-related gene expression. 展开更多
关键词 Amino acid metabolism Antioxidant CHICKEN enteromorpha prolifera Lipid metabolism Oxidative stress
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