Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not mee...Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.展开更多
Acetonitrile inorganic salt aqueous two-phase extraction method was established for the determination of triazine herbicides(simeton,cyanazine,desmetryn,terbumeton,terbuthylazine and dimethametryn)in environmental wat...Acetonitrile inorganic salt aqueous two-phase extraction method was established for the determination of triazine herbicides(simeton,cyanazine,desmetryn,terbumeton,terbuthylazine and dimethametryn)in environmental water samples by high-performance liquid chromatography(HPLC).The extraction solvents types and volume of acetonitrile,the inorganic salt types and amount of(NH_(4))_(2)SO_(4),extraction time and pH of sample solution were optimized by single-factor experiment and central composite design.Under the optimum extraction conditions,all linear ranges were obtained with coefficients of determination(r)≥0.9993.The limits of detection for this proposed method were in the range of 0.16-0.28μg/L for six triazine herbicides.The developed method has been successfully applied to the analysis of target triazine herbicides from lake,canal and moat in real-world water samples.The recoveries of target analytes were in the range from 87.0 to 110.9%and the relative standard deviation was lower than 7.3%.展开更多
The paper describes a novel method for copper preconcentration using microcrystalline triphenylmethane loaded with malachite green prior to the determination by the flame atomic absorption spectrometry （FAAS）. Under...The paper describes a novel method for copper preconcentration using microcrystalline triphenylmethane loaded with malachite green prior to the determination by the flame atomic absorption spectrometry （FAAS）. Under the optimum conditions, Cu（Ⅱ） can be totally adsorbed on the surface of microcrystalline triphenylmethane, and completely separated from Pb（Ⅱ）, Cd（Ⅱ）, Co（Ⅱ）, Cr（Ⅲ）, Ni（Ⅱ）, Mn（Ⅱ）, Fe（Ⅲ） and Al（Ⅲ） by controlling acidity. The preconcentration factor of this proposed method is 200. The recovery is in a range of 97.5%-105%. The relative standard deviation （RSD） is not beyond 3.0%. The proposed method has been successfully applied to the determination of trace copper in various water samples with satisfactory results.展开更多
基金Supported by the Basic Scientific Fund for National Public Research Institutes of China(Nos.GY02-2011T10,2015P07)the Qingdao Talent Program(No.13-CX-20)+1 种基金the National Natural Science Foundation of China(Nos.31100567,41176061)the National Natural Science Foundation for Creative Groups(No.41521064)
文摘Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.
基金the major project of Natural Science Foundation of Jiangsu Higher Education Institutions of China(no:15KJA610003)Natural Science Foundation of Jiangsu Province(no.BK20160359)Natural Science and Technology Program on Water Pollution Control and Treatment(2017ZX07205-2).
文摘Acetonitrile inorganic salt aqueous two-phase extraction method was established for the determination of triazine herbicides(simeton,cyanazine,desmetryn,terbumeton,terbuthylazine and dimethametryn)in environmental water samples by high-performance liquid chromatography(HPLC).The extraction solvents types and volume of acetonitrile,the inorganic salt types and amount of(NH_(4))_(2)SO_(4),extraction time and pH of sample solution were optimized by single-factor experiment and central composite design.Under the optimum extraction conditions,all linear ranges were obtained with coefficients of determination(r)≥0.9993.The limits of detection for this proposed method were in the range of 0.16-0.28μg/L for six triazine herbicides.The developed method has been successfully applied to the analysis of target triazine herbicides from lake,canal and moat in real-world water samples.The recoveries of target analytes were in the range from 87.0 to 110.9%and the relative standard deviation was lower than 7.3%.
基金Project supported by the Natural Science Foundation of Henan Province （No. 0511020500） and Innovation Project Foundation of University in Henan Province （No. 2001-513）.
文摘The paper describes a novel method for copper preconcentration using microcrystalline triphenylmethane loaded with malachite green prior to the determination by the flame atomic absorption spectrometry （FAAS）. Under the optimum conditions, Cu（Ⅱ） can be totally adsorbed on the surface of microcrystalline triphenylmethane, and completely separated from Pb（Ⅱ）, Cd（Ⅱ）, Co（Ⅱ）, Cr（Ⅲ）, Ni（Ⅱ）, Mn（Ⅱ）, Fe（Ⅲ） and Al（Ⅲ） by controlling acidity. The preconcentration factor of this proposed method is 200. The recovery is in a range of 97.5%-105%. The relative standard deviation （RSD） is not beyond 3.0%. The proposed method has been successfully applied to the determination of trace copper in various water samples with satisfactory results.
