Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural l...Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural locations in Laborecká vrchovina (Slovakia). Samples were collected by destructive methods in all three locations. Silicon content was determined in dry biomass by AAS. Silicon content in plants ranged from 21.11 ± 3.24 g·kg-1 to 32.80 ± 8.03 g·kg-1. The highest content of silicon exhibited samples of the September collection. We found that the location and the year in terms of silicon content were not statistically significant. The main sources for statistical variability in the accumulation of silicon were during the collections.展开更多
Three new phenolic glycosides, named as equisetumoside A (3-methoxy-11,12-dihydroxy-phenylhexane-9- one-4-O-beta -D-glucopyranoside), equisetumoside B (3-methoxy-4,11-dihydroxy-phenylhexane-9-one12-O-beta -D-glucopyra...Three new phenolic glycosides, named as equisetumoside A (3-methoxy-11,12-dihydroxy-phenylhexane-9- one-4-O-beta -D-glucopyranoside), equisetumoside B (3-methoxy-4,11-dihydroxy-phenylhexane-9-one12-O-beta -D-glucopyranoside) and equisetumoside C ( cis-ferulic acid potassium salt 4-O-beta -D-glucopyranoside) were isolated from the water-soluble extract of fertile sprouts of Equisetum, arvense L. (Equisetaceae), together with uridine, inosine, 2'-deoxyinosine, 2'-deoxycytidine, tryptophan, thymidine, 5-carboxy-2'-deoxyuridine, coniferin, and kaempferol 3-O-beta -D-sophoroside-7-O-beta -D-glucopyranoside. Their structures were elucidated by spectroscopic analysis and chemical transformation.展开更多
Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatme...Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatment possibilities. Aim: The aim of our study was to investigate renal morphology and functioning after 12 weeks of treatment with Equisetum arvense and Viscum album. Methods: Male Wistar rats with 5/6 nephrectomy received herbal drug preparation Equisetum/Viscum in a dosage of 0.007 g/kg/die (herbal group) or losartan (180 mg/l, ARB group) or remained untreated. Systolic blood pressure (SBP) and proteinuria were measured. Renal cortex tissue samples examined for focal-segmental glomerulosclerosis (FSGS) and interstitial fibrosis (IF). mRNA was isolated to estimate CCL2/MCP-1 gene transcription. Results: SBP was significantly lower both in herbal group and ARB group compared with untreated animals (p between ARB group and untreated NPX (p = 0.001). Quantitative RT-PCR analysis revealed statistically significant differences of mRNA transcription for MCP-1 between herbal group and untreated group (p Conclusions: We report about beneficial impact of horsetail Equisetum arvense and mistletoe Viscum album treatment on kidney functioning and morphology.展开更多
[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm...[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.展开更多
基金The work was supported by the Agency of Ministry of Education,Science,Research and Sport of the Slovak Republic,the project:00162-0001(MS SR-3634/2010-11).
文摘Horsetail (Equisetum arvense L.) is a perennial herb which creates during the life cycle spring and summer stems. The selected species and populations were monitored in the years 2009-2011 in three different natural locations in Laborecká vrchovina (Slovakia). Samples were collected by destructive methods in all three locations. Silicon content was determined in dry biomass by AAS. Silicon content in plants ranged from 21.11 ± 3.24 g·kg-1 to 32.80 ± 8.03 g·kg-1. The highest content of silicon exhibited samples of the September collection. We found that the location and the year in terms of silicon content were not statistically significant. The main sources for statistical variability in the accumulation of silicon were during the collections.
文摘Three new phenolic glycosides, named as equisetumoside A (3-methoxy-11,12-dihydroxy-phenylhexane-9- one-4-O-beta -D-glucopyranoside), equisetumoside B (3-methoxy-4,11-dihydroxy-phenylhexane-9-one12-O-beta -D-glucopyranoside) and equisetumoside C ( cis-ferulic acid potassium salt 4-O-beta -D-glucopyranoside) were isolated from the water-soluble extract of fertile sprouts of Equisetum, arvense L. (Equisetaceae), together with uridine, inosine, 2'-deoxyinosine, 2'-deoxycytidine, tryptophan, thymidine, 5-carboxy-2'-deoxyuridine, coniferin, and kaempferol 3-O-beta -D-sophoroside-7-O-beta -D-glucopyranoside. Their structures were elucidated by spectroscopic analysis and chemical transformation.
文摘Background: Chronic kidney disease (CKD) is an irreversible decline in the glomerular filtration due to nephrosclerosis and glomerular loss. Rat remnant kidney model enables to assess the benefits of different treatment possibilities. Aim: The aim of our study was to investigate renal morphology and functioning after 12 weeks of treatment with Equisetum arvense and Viscum album. Methods: Male Wistar rats with 5/6 nephrectomy received herbal drug preparation Equisetum/Viscum in a dosage of 0.007 g/kg/die (herbal group) or losartan (180 mg/l, ARB group) or remained untreated. Systolic blood pressure (SBP) and proteinuria were measured. Renal cortex tissue samples examined for focal-segmental glomerulosclerosis (FSGS) and interstitial fibrosis (IF). mRNA was isolated to estimate CCL2/MCP-1 gene transcription. Results: SBP was significantly lower both in herbal group and ARB group compared with untreated animals (p between ARB group and untreated NPX (p = 0.001). Quantitative RT-PCR analysis revealed statistically significant differences of mRNA transcription for MCP-1 between herbal group and untreated group (p Conclusions: We report about beneficial impact of horsetail Equisetum arvense and mistletoe Viscum album treatment on kidney functioning and morphology.
文摘[Objectives] To establish Thlaspi arvense L. HPLC fingerprints,to provide reference for quality evaluation Thlaspi arvense L.[Methods]HPLC chromatography was used,HPLC column Acclaim~ 120C_(18)( 250 mm × 3. 0 mm,5 μm),mobile phase of acetonitrile-0. 2% phosphoric acid aqueous solution gradient elution,flow rate of 0. 7 m L/min,column temperature of 30 ℃,detection wavelength of 320nm; the chromatographic fingerprint similarity evaluation software( Version 2004 A) was used for similarity evaluation and data processing on 10 origin Thlaspi arvense L. spectra,and the median method was used to generate control Thlaspi arvense L. fingerprint. [Results] The similarity of 10 origin Thlaspi arvense L. spectra was greater than 0. 90,19 common peaks were separated from different origin Thlaspi arvense L. and isovitexin,apigenin,luteolin and acacetin were identified. [Conclusions] The established HPLC fingerprint contained a lot of information and showed good specificity for Thlaspi arvense L.,which can provide a scientific basis for Thlaspi arvense L. quality evaluation system.
