Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of th...Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining展开更多
Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The ...Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly,Drosophila melanogaster.It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies,especially during the larval stages.When 320 mg L-1 copper-contaminated food was used,most of the larvae died when they were small and before the pupal stage.The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper.The small-molecular-weight protein bands gradually became weaker or were lost as the copper levels increased.However,low doses of copper stimulated a stronger expression of a few proteins.These results indicate that low doses of copper generally have no lethal effects on D.melanogaster because a specific group of genes,which encode specific proteins,are probably activated in order to withstand the onslaught of stressful conditions.At high doses of copper in food(e.g.320 mg L-1),fly development and viability are significantly affected.展开更多
EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong diff...EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.展开更多
[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ t...[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.展开更多
文摘Almost all previous reports on plant esterase(EST)isoenzyme analysis appeared to have ac-cepted that the relative staining strength(RSS)ofelectrophoretical bands represented the relative ac-tivities and contents of the correspondingisoenzymes.Nevertheless,we found in studyingthat the RSS changed with different staining
基金supported by the National Natural Science foundation of China (No.30300035)by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry[No. (2004)527].
文摘Copper is an important ingredient in fungicides,which are used for many economic plants.However,there is concern about the side-effects of copper-based fungicides due to their potential to affect beneficial mites.The purpose of the present paper was to investigate the effects of copper on the development of the fruit fly,Drosophila melanogaster.It was found that higher doses of copper significantly prolonged the developmental time of the fruit flies,especially during the larval stages.When 320 mg L-1 copper-contaminated food was used,most of the larvae died when they were small and before the pupal stage.The protein contents and esterase isozymes extracted from the larvae changed according to the doses of copper.The small-molecular-weight protein bands gradually became weaker or were lost as the copper levels increased.However,low doses of copper stimulated a stronger expression of a few proteins.These results indicate that low doses of copper generally have no lethal effects on D.melanogaster because a specific group of genes,which encode specific proteins,are probably activated in order to withstand the onslaught of stressful conditions.At high doses of copper in food(e.g.320 mg L-1),fly development and viability are significantly affected.
文摘EST isozymes are one of the frequently used biochemical markers in genetic analysis of fungi. and the staining is an important process in electrophoresis analysis of studying Est.The effects were compared ainong different stain recipes for Est of 3 kinds of fungi-Lentinus edodes. Pleurotus sapidus. Phellinus igriarius and 2 kinds of plants-Populus sp and Brassica chinensis. Of the four kinds of Est staining recipes tested.the recipe α-acetic acid-naphther showed the best effect.and followed by β-aceticacid-naphther, semicontent α-aceticacid-naphther and α+β-aceticacidnathpher.
基金Supported by National Science and Technology Support Program"Regional Development of Chinese Traditional Medicine Industry and Research of Characteristic Product"(2006BIA06A20)Personnel Services Business Action Plan of Ministry ofScience and Technology "Development of Series Products of Rare Edible Fungus"(2009GJD20012)~~
文摘[Objective] The mating genotype was studied and compared with esterase isozyme of Ganoderma lucidum populations between groups in order to clarify their differences in genetic relationship analysis. [Method] OWE-SOJ technique was applied to identify standard mating types and determinate mating genotype between groups of monokaryons isolates from 24 G. lucidum stains. Genetic relationships were analyzed by combined group mating genotype determination with esterase isozyme assay. [Result] All strains of G. lucidum could be divided into 7 large groups of the mating genotype. Four alleles of A factor, four alleles of B factor and one mixed alleles of A factor were found in this study. Distorted segregation ratio among monokaryon mycelia of G. lucidum had been observed in four kinds of mat- ing types to some extent. Twenty-eight different types of enzyme bands were determined in esterase isozyme test, Twenty-four strains of G. lucidum could be divided into 9 large groups through the cluster analysis when the genetic similarity coefficient was 0.73214. Comparing the results of mating genotype analysis and esterase isozyme analysis, it showed great similarity. [Conclusion] Mating genotype analysis could be used as an important supplementary method for strain identification and genetic diversity research.
