Graves' disease is an autoimmune disease, which can manifest with a variety of extrathyroidal clinical syndromes like ophthalmopathy, pretibial myxedema(dermopathy), acropathy, cardiomyopathy, and encephalopathy. ...Graves' disease is an autoimmune disease, which can manifest with a variety of extrathyroidal clinical syndromes like ophthalmopathy, pretibial myxedema(dermopathy), acropathy, cardiomyopathy, and encephalopathy. Though quite rare, this disease can also manifest with gastrointestinal symptoms such as dysphagia, heartburn, nausea, vomiting and diarrhea. We report a clinical case of Graves' disease manifesting with dysfunction of the esophagus and heartburn in a 61-year-old man. In the muscular layer of the esophagus we found dystrophic changes led to its atony, which was documented by endoscopy and high-resolution manometry. The pathology features of esophageal symptoms were: focal proliferation of the basal cells, vascular distension, and dystrophy of the epithelial cells. Antithyroid treatment led to decrease of all clinical symptoms after 5 d of Thiamazole administration. Complete restoration of peristalsis in the esophagus, according to manometry, was observed in 1 mo after initiation of treatment.展开更多
This current cross-sectional study investigates the relationship between thyroid hormones and peripheral artery disease （PAD） among euthyroid Chinese population aged 40 years and above. Serum free triiodothyronine ...This current cross-sectional study investigates the relationship between thyroid hormones and peripheral artery disease （PAD） among euthyroid Chinese population aged 40 years and above. Serum free triiodothyronine （FT3）, free thyroxin （FT4）, thyroid-stimulating hormone （TSH）, and thyroid antibodies were measured.展开更多
Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves’ disease(GD)and Graves’ disease combined with type 2 diabetes mellitus(GD/T2DM).Methods Fifteen cases of GD/T2DM w...Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves’ disease(GD)and Graves’ disease combined with type 2 diabetes mellitus(GD/T2DM).Methods Fifteen cases of GD/T2DM were selected from our hospital from November 2001 to November 2004.Before and after therapy thyroid function,thyroglobulin antibody(TGA),thyroid microsomal antibody(TMA)and blood glucose level were measured,and T lymphocyte subsets(CD3,CD4,CD8,CD4/CD8)and NK cells(CD56)were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry,respectively.At the same time,comparison was made with simple GD(15 cases),T2DM(15 cases)and healthy control(20 cases).Results Before therapy,CD4/CD8,CD4 and NK cells in GD/T2DM were less than normal,and there was no significant difference in comparison with simple GD(P<0.05).In T2DM group,only CD4/CD8 and CD4 were less than those of healthy controls(P<0.05).When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups,various indexes recovered,which were more obvious in simple GD.Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM,which is more significant than that of simple GD or T2DM.The recovery of thyroid function and immune abnormality is not consistent,and the recovery of GD is more significant than that of GD/T2DM.展开更多
TSAb and TSBAb both were measured in i63 patients with Graves' disease(GD) and 31 Autoimmune Thyroiditis(AIT) individually and simultaneously. The TSAb activities and positive percentage in active and relapse GD ...TSAb and TSBAb both were measured in i63 patients with Graves' disease(GD) and 31 Autoimmune Thyroiditis(AIT) individually and simultaneously. The TSAb activities and positive percentage in active and relapse GD groups were higher than those in the normal control and euthyroidgroups; no significant difference was found between the active GD and relapse GD groups. TSAb were positive in one of 31 Patients with hypothyroidism due to AIT. TSBAb were positive in GD patients after treatment who became patients with hypothyroidism and patients with bypothyroidism dueto AIT, being 30. 0% and 35.5% respectively. TSAb and TSBAb were measured simultaneously in 35GD patients with different thyroid function status, and 4 PatientS with hypothyroidism due to AIT.We found that two patients with GD who were euthyroid during treatment with ATD and one patient with active GD had TSAb and TSBAb coexisting in circulation. we would postulate that perhaps only one kind of preponderant autoantibody activity could be detected and those nonpreponderant autoantibodies might be very difficultly found, when the patients were in a certain stable thyroid function saute.展开更多
Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice wer...Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice were immunized by hTSH receptor expressing cells (DAP3.WT). 1-2×107 DAP3.WT cells were peri toneally injected into mice every two weeks for a total of six times. Two weeks after final immunization, mice were killed for measurement of total thyroxine, T RAb and pathological examination. Results The thyroxine level of the immunized Fcγ recept or gene knockout mice was significantly lower than that of the immunized wild ty pe control mice (2.2±0.31 vs. 3.32± 0.59 g·dL -1, P< 0.05 ),but there was no significant difference between immunized Fcγ R KO mic e and non-immunized wild type control group. The TRAb levels of the immunized F γ R KO mice significantly increased compared to those of the immunized wild type mice (21.75±8.21 vs. 14.11±6.21, P< 0.05). The lymphocyte cel ls infiltration and destruction of thyroid follicles were found in the thyroid gland of the immunized Fcγ R KO mice. Conclusion These results suggest that Fcγ receptor may be involved in the pathogenesis of Graves' disease.展开更多
Objective To construct an animal model of Graves’ disease(GD)by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex(MHC)class II molecules and human thyrotropin receptor(TSHR)molecul...Objective To construct an animal model of Graves’ disease(GD)by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex(MHC)class II molecules and human thyrotropin receptor(TSHR)molecules.Methods BALB/c mice in experimental group(H-2d)were immunized with hM12 cells intraperitoneally every 2 weeks for six times,while mice in control group were immunized with M12 cells.Five weeks later,the thyroids were histologically examined,and serum samples were tested for thyroid-stimulating antibodies(TSAb)and thyroid hormone levels.Results One BALB/c mouse in experimental group developed Graves’-like disease.Total T4 and T3 levels in this mouse were above the upper limit of normal,TSAb activity was displayed in its serum.The thyroid histologically showed the features of thyroid hyperactivity including thyrocyte hypercellularity and colloid ABSorption.None of control mice developed Graves’-like disease.Conclusion An animal model with some characteristics of human Graves’ disease was successfully induced and the model will facilitate studies aimed directly at understanding the pathogenesis of autoimmunity in Graves’ disease.展开更多
Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 eu...Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 euthyroid patients were consecutively enrolled and di- vided into CAD group （n = 3306） and non-CAD group （n = 900）. All patients underwent coronary angiography （CAG）. Gensini score （GS） was used to determine the severity of coronary artery stenosis. Severe CAD was defined as GS 〉 32 and mild CAD was defined as GS 〈 32. Logistic regression analysis and linear regression analysis were conducted to determine the association of FT3 with CAD in patients with different gender and ages. Results Concentration of FT3 was lower in patients with CAD than that in angiography-normal control group （P 〈 0.05）. In addition, concentration of FT3 was lower in severe CAD than that in mild CAD. After adjusting for traditional cardiovascular risk factors and potential confounders, FT3 was negatively correlated with the presence of CAD, but not in the old patients （〉 65 years old）. Mul- tivariable linear regression analysis showed that FT3 was negatively associated with GS in male and young patients with stable CAD, but not in the old patients. Conclusions Low FT3 within normal range was negatively associated with the presence and severity of CAD in young patients, but not in the old ones. Further studies are needed to confirm our findings.展开更多
Graves' disease,the production of thyroid-stimulating hormone receptor-stimulating antibodies leading to hyperthyroidism,is one of the most common forms of human autoimmune disease.It is widely agreed that complex...Graves' disease,the production of thyroid-stimulating hormone receptor-stimulating antibodies leading to hyperthyroidism,is one of the most common forms of human autoimmune disease.It is widely agreed that complex diseases are not controlled simply by an individual gene or DNA variation but by their combination.Single nucleotide polymorphisms(SNPs),which are the most common form of DNA variation,have great potential as a medical diagnostic tool.In this paper,the P-value is used as a SNP pre-selection criterion,and a wrapper algorithm with binary particle swarm optimization is used to find the rule for discriminating between affected and control subjects.We analyzed the association between combinations of SNPs and Graves' disease by investigating 108 SNPs in 384 cases and 652 controls.We evaluated our method by differentiating between cases and controls in a five-fold cross validation test,and it achieved a 72.9% prediction accuracy with a combination of 17 SNPs.The experimental results showed that SNPs,even those with a high P-value,have a greater effect on Graves' disease when acting in a combination.展开更多
INTRODUCTION Autoimmunity is defined as, a condition characterized by a specific humoral or cell-mediated immune response against the constituents of the body＇s own tissues （autoantigens）. In numerous autoimmune di...INTRODUCTION Autoimmunity is defined as, a condition characterized by a specific humoral or cell-mediated immune response against the constituents of the body＇s own tissues （autoantigens）. In numerous autoimmune diseases, such an immune response is well recognized that causes damage to the self-constituents of body tissues by the products of the immune system. Graves＇ hyperthyroidism occurs after the loss of tolerance to the thyroid stimulating hormone receptor （TSHR） and the generation of thyroid stimulatory antibodies that mimic the action of thyroid-stimulating hormone （TSH）.展开更多
Objective: To investigate the cellular and molecular mechanisms of some Chinese drugs on apoptosis of thyrocytes in Graves' disease. Methods: Two to ten weeks before and after using Chinese drugs on 13 Graves'...Objective: To investigate the cellular and molecular mechanisms of some Chinese drugs on apoptosis of thyrocytes in Graves' disease. Methods: Two to ten weeks before and after using Chinese drugs on 13 Graves' disease patients with anti-thyroid drugs, patients' thyroid tissue was aspired with percutaneous puncturing needle. The effects of some Chinese drugs on thyrocytes was studied by applying cell morphology (stained with nuclear fast red, NFR), flow cytometry (stained with propidium iodide, PI), and terminal uridine deoxynucleotidyl end-labelling (TUNEL) technique (alkaline phosphatase and CBIP/NBT, NFR). The size of thyroid gland was measured by color Doppler ultrasonography. Results: After Chinese drug treatment, compared with single anti-thyroid drug group, the size of thyroid was significantly reduced (P<0.01). The apoptosis of thyrocytes can be observed by cell morphology such as chromatin condensation and nuclear fragmentation. The apoptosis rate is 18.66%±20.01% (n=13) in anti-thyroid drugs plus Chinese drugs group, 2.11%±1.78% (n=13) in single anti-thyroid drug group (P<0.01). For up to 2-10 weeks, more TUNEL-positive cells were found after treatment with Chinese drugs than those treated with anti-thyroid drug alone. Conclusion: Some Chinese drugs when used in combination with anti-thyroid drugs in treating Graves' disease could induce apoptosis of thyrocytes.展开更多
文摘Graves' disease is an autoimmune disease, which can manifest with a variety of extrathyroidal clinical syndromes like ophthalmopathy, pretibial myxedema(dermopathy), acropathy, cardiomyopathy, and encephalopathy. Though quite rare, this disease can also manifest with gastrointestinal symptoms such as dysphagia, heartburn, nausea, vomiting and diarrhea. We report a clinical case of Graves' disease manifesting with dysfunction of the esophagus and heartburn in a 61-year-old man. In the muscular layer of the esophagus we found dystrophic changes led to its atony, which was documented by endoscopy and high-resolution manometry. The pathology features of esophageal symptoms were: focal proliferation of the basal cells, vascular distension, and dystrophy of the epithelial cells. Antithyroid treatment led to decrease of all clinical symptoms after 5 d of Thiamazole administration. Complete restoration of peristalsis in the esophagus, according to manometry, was observed in 1 mo after initiation of treatment.
基金supported by the National Natural Science Foundation of China(No.81370960 and 81670795)the Ministry of Science and Technology(No.2015BAI12B14,2015BAI12B02,2016YFC0901200,and 2016YFC1305202)
文摘This current cross-sectional study investigates the relationship between thyroid hormones and peripheral artery disease （PAD） among euthyroid Chinese population aged 40 years and above. Serum free triiodothyronine （FT3）, free thyroxin （FT4）, thyroid-stimulating hormone （TSH）, and thyroid antibodies were measured.
基金the Scientific and Technological Development Foundation of Baotou Medical Science in Inner Mongolia [(2001) No.198]
文摘Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves’ disease(GD)and Graves’ disease combined with type 2 diabetes mellitus(GD/T2DM).Methods Fifteen cases of GD/T2DM were selected from our hospital from November 2001 to November 2004.Before and after therapy thyroid function,thyroglobulin antibody(TGA),thyroid microsomal antibody(TMA)and blood glucose level were measured,and T lymphocyte subsets(CD3,CD4,CD8,CD4/CD8)and NK cells(CD56)were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry,respectively.At the same time,comparison was made with simple GD(15 cases),T2DM(15 cases)and healthy control(20 cases).Results Before therapy,CD4/CD8,CD4 and NK cells in GD/T2DM were less than normal,and there was no significant difference in comparison with simple GD(P<0.05).In T2DM group,only CD4/CD8 and CD4 were less than those of healthy controls(P<0.05).When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups,various indexes recovered,which were more obvious in simple GD.Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM,which is more significant than that of simple GD or T2DM.The recovery of thyroid function and immune abnormality is not consistent,and the recovery of GD is more significant than that of GD/T2DM.
文摘TSAb and TSBAb both were measured in i63 patients with Graves' disease(GD) and 31 Autoimmune Thyroiditis(AIT) individually and simultaneously. The TSAb activities and positive percentage in active and relapse GD groups were higher than those in the normal control and euthyroidgroups; no significant difference was found between the active GD and relapse GD groups. TSAb were positive in one of 31 Patients with hypothyroidism due to AIT. TSBAb were positive in GD patients after treatment who became patients with hypothyroidism and patients with bypothyroidism dueto AIT, being 30. 0% and 35.5% respectively. TSAb and TSBAb were measured simultaneously in 35GD patients with different thyroid function status, and 4 PatientS with hypothyroidism due to AIT.We found that two patients with GD who were euthyroid during treatment with ATD and one patient with active GD had TSAb and TSBAb coexisting in circulation. we would postulate that perhaps only one kind of preponderant autoantibody activity could be detected and those nonpreponderant autoantibodies might be very difficultly found, when the patients were in a certain stable thyroid function saute.
文摘Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice were immunized by hTSH receptor expressing cells (DAP3.WT). 1-2×107 DAP3.WT cells were peri toneally injected into mice every two weeks for a total of six times. Two weeks after final immunization, mice were killed for measurement of total thyroxine, T RAb and pathological examination. Results The thyroxine level of the immunized Fcγ recept or gene knockout mice was significantly lower than that of the immunized wild ty pe control mice (2.2±0.31 vs. 3.32± 0.59 g·dL -1, P< 0.05 ),but there was no significant difference between immunized Fcγ R KO mic e and non-immunized wild type control group. The TRAb levels of the immunized F γ R KO mice significantly increased compared to those of the immunized wild type mice (21.75±8.21 vs. 14.11±6.21, P< 0.05). The lymphocyte cel ls infiltration and destruction of thyroid follicles were found in the thyroid gland of the immunized Fcγ R KO mice. Conclusion These results suggest that Fcγ receptor may be involved in the pathogenesis of Graves' disease.
基金supported by the National Natural Science Foundation of China(No.30371335)
文摘Objective To construct an animal model of Graves’ disease(GD)by immunizing BALB/c mice with hM12 cells co-expressing major histocompatibility complex(MHC)class II molecules and human thyrotropin receptor(TSHR)molecules.Methods BALB/c mice in experimental group(H-2d)were immunized with hM12 cells intraperitoneally every 2 weeks for six times,while mice in control group were immunized with M12 cells.Five weeks later,the thyroids were histologically examined,and serum samples were tested for thyroid-stimulating antibodies(TSAb)and thyroid hormone levels.Results One BALB/c mouse in experimental group developed Graves’-like disease.Total T4 and T3 levels in this mouse were above the upper limit of normal,TSAb activity was displayed in its serum.The thyroid histologically showed the features of thyroid hyperactivity including thyrocyte hypercellularity and colloid ABSorption.None of control mice developed Graves’-like disease.Conclusion An animal model with some characteristics of human Graves’ disease was successfully induced and the model will facilitate studies aimed directly at understanding the pathogenesis of autoimmunity in Graves’ disease.
文摘Objective To study whether free triiodothyronine （FT3） within normal range has effects on the presence and severity of coronary ar- tery disease （CAD） in different gender and age groups. Methods A total of 4206 euthyroid patients were consecutively enrolled and di- vided into CAD group （n = 3306） and non-CAD group （n = 900）. All patients underwent coronary angiography （CAG）. Gensini score （GS） was used to determine the severity of coronary artery stenosis. Severe CAD was defined as GS 〉 32 and mild CAD was defined as GS 〈 32. Logistic regression analysis and linear regression analysis were conducted to determine the association of FT3 with CAD in patients with different gender and ages. Results Concentration of FT3 was lower in patients with CAD than that in angiography-normal control group （P 〈 0.05）. In addition, concentration of FT3 was lower in severe CAD than that in mild CAD. After adjusting for traditional cardiovascular risk factors and potential confounders, FT3 was negatively correlated with the presence of CAD, but not in the old patients （〉 65 years old）. Mul- tivariable linear regression analysis showed that FT3 was negatively associated with GS in male and young patients with stable CAD, but not in the old patients. Conclusions Low FT3 within normal range was negatively associated with the presence and severity of CAD in young patients, but not in the old ones. Further studies are needed to confirm our findings.
基金supported by the National Natural Science Foundation of China (Grant No. 60774086)the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20090201110027)
文摘Graves' disease,the production of thyroid-stimulating hormone receptor-stimulating antibodies leading to hyperthyroidism,is one of the most common forms of human autoimmune disease.It is widely agreed that complex diseases are not controlled simply by an individual gene or DNA variation but by their combination.Single nucleotide polymorphisms(SNPs),which are the most common form of DNA variation,have great potential as a medical diagnostic tool.In this paper,the P-value is used as a SNP pre-selection criterion,and a wrapper algorithm with binary particle swarm optimization is used to find the rule for discriminating between affected and control subjects.We analyzed the association between combinations of SNPs and Graves' disease by investigating 108 SNPs in 384 cases and 652 controls.We evaluated our method by differentiating between cases and controls in a five-fold cross validation test,and it achieved a 72.9% prediction accuracy with a combination of 17 SNPs.The experimental results showed that SNPs,even those with a high P-value,have a greater effect on Graves' disease when acting in a combination.
基金Financial support and sponsorship This study was supported by the grants from National Natural Science Foundation of China （No. 81170729 and No. 81200574）.
文摘INTRODUCTION Autoimmunity is defined as, a condition characterized by a specific humoral or cell-mediated immune response against the constituents of the body＇s own tissues （autoantigens）. In numerous autoimmune diseases, such an immune response is well recognized that causes damage to the self-constituents of body tissues by the products of the immune system. Graves＇ hyperthyroidism occurs after the loss of tolerance to the thyroid stimulating hormone receptor （TSHR） and the generation of thyroid stimulatory antibodies that mimic the action of thyroid-stimulating hormone （TSH）.
文摘Objective: To investigate the cellular and molecular mechanisms of some Chinese drugs on apoptosis of thyrocytes in Graves' disease. Methods: Two to ten weeks before and after using Chinese drugs on 13 Graves' disease patients with anti-thyroid drugs, patients' thyroid tissue was aspired with percutaneous puncturing needle. The effects of some Chinese drugs on thyrocytes was studied by applying cell morphology (stained with nuclear fast red, NFR), flow cytometry (stained with propidium iodide, PI), and terminal uridine deoxynucleotidyl end-labelling (TUNEL) technique (alkaline phosphatase and CBIP/NBT, NFR). The size of thyroid gland was measured by color Doppler ultrasonography. Results: After Chinese drug treatment, compared with single anti-thyroid drug group, the size of thyroid was significantly reduced (P<0.01). The apoptosis of thyrocytes can be observed by cell morphology such as chromatin condensation and nuclear fragmentation. The apoptosis rate is 18.66%±20.01% (n=13) in anti-thyroid drugs plus Chinese drugs group, 2.11%±1.78% (n=13) in single anti-thyroid drug group (P<0.01). For up to 2-10 weeks, more TUNEL-positive cells were found after treatment with Chinese drugs than those treated with anti-thyroid drug alone. Conclusion: Some Chinese drugs when used in combination with anti-thyroid drugs in treating Graves' disease could induce apoptosis of thyrocytes.
