An automatic method able to recognize a presented section through the biparietal plane of the fetal head and a section through the fetal femur in ultrasound images is developed. Once the correct anatomical section for...An automatic method able to recognize a presented section through the biparietal plane of the fetal head and a section through the fetal femur in ultrasound images is developed. Once the correct anatomical section for measurement is identified by the machine, the placement of the measurement calipers is automatically determined by fitting an active contour model to the structure of interest. The fetal biparietal diameter (BPD) and femur length (FL) are then measured automatically. The validation data set contained 167 and 197 B-mode images for BPD and FL measurements, respectively. The images were acquired using 4 different ultrasound scanners, which resulted in varied image quality and gain settings. The mean gestational age (GA) of the fetuses was 19.4 weeks, range 16 to 41 weeks. A measurement success rate of 90% was achieved for both BPD and FL. The correlation coefficients between the manual and automatic measurements were 0.995 (BPD) and 0.967 (FL), mean errors were 0.5 mm (BPD) and -1.7 mm (FL) and error range with 95% confidence interval (CI) were ﹣3.8 - 4.8 mm (BPD) and ﹣11.4 - 8.1 mm (FL). The automatic measurement results were consistent in both high and low gain settings. The intraclass correlation coefficients between manual and automatic measurements were 0.995 (95% CI;0.981 - 0.999) for BPD in high gain, 1.0 (95% CI;0.998 - 1.0) for BPD in low gain, 0.998 (95% CI;0.991 - 0.999) for FL in high gain and 0.999 (95% CI;0.996 - 1.0) for FL in low gain settings. The method was implemented on a prototype, portable ultrasound machine designed to be used in low- and middle-income countries (LMIC). The overall performance of the method supports our hypothesis that automated methods can be used and are beneficial in a clinical setting.展开更多
目的:探讨载脂蛋白A1、B基因多态性对非创伤性股骨头坏死(avascular necrosis of the femoral head,ANFH)发生的影响。方法:应用聚合酶链反应对中国北方汉族143例ANFH患者和92例正常人分别扩增含ApoA1基因启动子-75bp和第一内含子+83bp...目的:探讨载脂蛋白A1、B基因多态性对非创伤性股骨头坏死(avascular necrosis of the femoral head,ANFH)发生的影响。方法:应用聚合酶链反应对中国北方汉族143例ANFH患者和92例正常人分别扩增含ApoA1基因启动子-75bp和第一内含子+83bp及ApoB基因EcoRI、XbaI和3'!VNTR的DNA片段,限制性内切酶酶切扩增产物,琼脂糖凝胶电泳分离基因多态性。结果:ApoA1基因启动子-75bp处,ANFH患者中A/A基因型频率明显高于正常组(P<0.01),而G/A基因型频率明显低于正常组(P<0.01)。ApoA1内含子+83bp位点,ApoB基因EcoRI、XbaI位点和3'!VNTR区域ANFH患者组和正常组基因型及等位基因频率分布无统计学差异。结论:ApoA1基因启动子区域-75bp位点A/A型可能是非创伤性股骨头坏死易感基因之一,但未能发现ApoA1第一内含子+83bp位点及ApoB基因EcoRI、XbaI和3'!VNTR位点多态性与非创伤性股骨头坏死发生有明显的关系。展开更多
文摘An automatic method able to recognize a presented section through the biparietal plane of the fetal head and a section through the fetal femur in ultrasound images is developed. Once the correct anatomical section for measurement is identified by the machine, the placement of the measurement calipers is automatically determined by fitting an active contour model to the structure of interest. The fetal biparietal diameter (BPD) and femur length (FL) are then measured automatically. The validation data set contained 167 and 197 B-mode images for BPD and FL measurements, respectively. The images were acquired using 4 different ultrasound scanners, which resulted in varied image quality and gain settings. The mean gestational age (GA) of the fetuses was 19.4 weeks, range 16 to 41 weeks. A measurement success rate of 90% was achieved for both BPD and FL. The correlation coefficients between the manual and automatic measurements were 0.995 (BPD) and 0.967 (FL), mean errors were 0.5 mm (BPD) and -1.7 mm (FL) and error range with 95% confidence interval (CI) were ﹣3.8 - 4.8 mm (BPD) and ﹣11.4 - 8.1 mm (FL). The automatic measurement results were consistent in both high and low gain settings. The intraclass correlation coefficients between manual and automatic measurements were 0.995 (95% CI;0.981 - 0.999) for BPD in high gain, 1.0 (95% CI;0.998 - 1.0) for BPD in low gain, 0.998 (95% CI;0.991 - 0.999) for FL in high gain and 0.999 (95% CI;0.996 - 1.0) for FL in low gain settings. The method was implemented on a prototype, portable ultrasound machine designed to be used in low- and middle-income countries (LMIC). The overall performance of the method supports our hypothesis that automated methods can be used and are beneficial in a clinical setting.
文摘目的:探讨载脂蛋白A1、B基因多态性对非创伤性股骨头坏死(avascular necrosis of the femoral head,ANFH)发生的影响。方法:应用聚合酶链反应对中国北方汉族143例ANFH患者和92例正常人分别扩增含ApoA1基因启动子-75bp和第一内含子+83bp及ApoB基因EcoRI、XbaI和3'!VNTR的DNA片段,限制性内切酶酶切扩增产物,琼脂糖凝胶电泳分离基因多态性。结果:ApoA1基因启动子-75bp处,ANFH患者中A/A基因型频率明显高于正常组(P<0.01),而G/A基因型频率明显低于正常组(P<0.01)。ApoA1内含子+83bp位点,ApoB基因EcoRI、XbaI位点和3'!VNTR区域ANFH患者组和正常组基因型及等位基因频率分布无统计学差异。结论:ApoA1基因启动子区域-75bp位点A/A型可能是非创伤性股骨头坏死易感基因之一,但未能发现ApoA1第一内含子+83bp位点及ApoB基因EcoRI、XbaI和3'!VNTR位点多态性与非创伤性股骨头坏死发生有明显的关系。
