[Objective] To investigate the effects of amino acid powder, fermented rapeseed meal and fermented cottonseed meal on the growth performance, amino acid absorption, body composition and liver function of allogynogenti...[Objective] To investigate the effects of amino acid powder, fermented rapeseed meal and fermented cottonseed meal on the growth performance, amino acid absorption, body composition and liver function of allogynogentic crucian carp. [ Method] The allogynogentic crucian carps with the average initial weight of 17.3 g were divided into control group and six experimental groups randomly. The allogynogentic crucian carps in the control group and experimental groups were fed a basal diet and the basal diets supplemented by amino acid powder, fermented rapeseed meal and fermented cottonseed meal, respectively. The growth rate and specific growth rate of allogynogentic crucian carps, the content of amino acids in serum and feeds, the composition indicators of body, muscle and liver, as well as the activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in liver and serum were determined, respectively. [ Result] The growth rate and specific growth rate (SGR) of allogynogentic crucian carps in the 3% amino acid powder group and the 6% fermented cottonseed meal group were increased greatly ( P 〈 0.05), and no significant difference was found between experimental group and control group. The serum lysine content of the 3% amino acid powder group and the 6% fermented cottonseed meal group was higher than that of the control group. In the experimental groups, the absorption of four essential amino acids was up to a peak at 4 h after feeding, which was consistent with the control group. The body composition and liver function of allogynogentic crucian carps had no significant difference between the experimental groups and the control group. [ Conclusion] The 3% amino acid powder, 6% fermented cottonseed meal and 6% fermented rapeseed meal can substitute fish meal, cottonseed meal and rapeseed meal to improve the clrowth performance, amino acid absorption of the allogynogentic crucian carps.展开更多
To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapese...To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapeseed meal diet(RSD),and fermented rapeseed meal diet(FRSD).Results showed that compared with RSD,FRSD feeding increased the average daily gain and final body weight in pigs(P<0.01).Compared with RSD feeding,FRSD feeding elevated the apparent digestibility of crude protein,acid detergent fiber,and ether extract in pigs(P<0.01).Moreover,the FRSD group exhibited greater apparent ileal digestibility of His,Thr,Lys,and Ser than the RSD group(P<0.01).The digestible energy,metabolic energy,and nitrogen utilization were higher in the FRSD and CSD groups than in the RSD group(P<0.01).As compared to the RSD,FRSD feeding decreased the serum concentration of leptin but significantly increased the concentrations of immunoglobulin(Ig)A,IgG,ghrelin,and enzyme activities of amylase,lipase,and trypsin in the pancreas(P<0.05).Interestingly,the villus height,the ratio of villus height to crypt depth,and the activities of brush border enzymes(e.g.,maltase and sucrase)in the small intestine were higher in the CSD and FRSD groups than in the RSD group(P<0.05).As compared to the RSD,the FRSD feeding not only increased the expression level of the occludin in the small intestinal epithelium(P<0.05)but also elevated the expression levels of claudin-1,MUC1,and PepT1 genes in the duodenum,and elevated the expression levels of SGLT1 and CAT1 genes in the jejunum(P<0.05).Importantly,FRSD feeding significantly decreased the abundance of Escherichia coli,but increased the abundance of Lactobacillus and the content of butyrate in the cecum and colon(P<0.05).These results indicated that compared with rapeseed meal,fermented rapeseed meal exhibited a positive effect on improving the growth performance and intestinal health in growing pigs,and the results may also help develop novel protein sources for animal nutrition and the feed industry.展开更多
Methionine (Met) and lysine (Lys) have been reported as the first two limiting amino acids (AA) for maximum milk yield and milk protein production. Supplying these AA may improve microbial protein synthesis and ...Methionine (Met) and lysine (Lys) have been reported as the first two limiting amino acids (AA) for maximum milk yield and milk protein production. Supplying these AA may improve microbial protein synthesis and therefore improve milk production without adding excess N to the environment. This observation utilized fermented soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM) and corn by Bacillus subtilis 168 and Leuconostoc mesenteroides as core feedstuffs to produce special biological protein feed for dairy cow. The results showed that the milk production, milk protein percentage, milk fat percentage and milk DM percentage of test groups in trial period were significantly more than those of the control group (P〈0.01), the results showed that adding fermenting protein feed in dairy cow diets could significantly improve milk yield, milk protein and milk fat content. The economic benefits of actual application were analyzed, the group of 0.5% was the best compared with the other groups.展开更多
基金supported by the Applied Basic Research Projects of Suzhou City (YJG0912)
文摘[Objective] To investigate the effects of amino acid powder, fermented rapeseed meal and fermented cottonseed meal on the growth performance, amino acid absorption, body composition and liver function of allogynogentic crucian carp. [ Method] The allogynogentic crucian carps with the average initial weight of 17.3 g were divided into control group and six experimental groups randomly. The allogynogentic crucian carps in the control group and experimental groups were fed a basal diet and the basal diets supplemented by amino acid powder, fermented rapeseed meal and fermented cottonseed meal, respectively. The growth rate and specific growth rate of allogynogentic crucian carps, the content of amino acids in serum and feeds, the composition indicators of body, muscle and liver, as well as the activities of glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in liver and serum were determined, respectively. [ Result] The growth rate and specific growth rate (SGR) of allogynogentic crucian carps in the 3% amino acid powder group and the 6% fermented cottonseed meal group were increased greatly ( P 〈 0.05), and no significant difference was found between experimental group and control group. The serum lysine content of the 3% amino acid powder group and the 6% fermented cottonseed meal group was higher than that of the control group. In the experimental groups, the absorption of four essential amino acids was up to a peak at 4 h after feeding, which was consistent with the control group. The body composition and liver function of allogynogentic crucian carps had no significant difference between the experimental groups and the control group. [ Conclusion] The 3% amino acid powder, 6% fermented cottonseed meal and 6% fermented rapeseed meal can substitute fish meal, cottonseed meal and rapeseed meal to improve the clrowth performance, amino acid absorption of the allogynogentic crucian carps.
基金This work was funded by the Key Research and Development Program of Sichuan Province(no.2020YFN0147)the National Natural Science Foundation of China(no.31972599).
文摘To explore the effects of fermented rapeseed meal(FRSM)on growth performance and intestinal health,a total of 30 growing pigs were randomly allotted to three treatments consisting of corn-soybean meal diet(CSD),rapeseed meal diet(RSD),and fermented rapeseed meal diet(FRSD).Results showed that compared with RSD,FRSD feeding increased the average daily gain and final body weight in pigs(P<0.01).Compared with RSD feeding,FRSD feeding elevated the apparent digestibility of crude protein,acid detergent fiber,and ether extract in pigs(P<0.01).Moreover,the FRSD group exhibited greater apparent ileal digestibility of His,Thr,Lys,and Ser than the RSD group(P<0.01).The digestible energy,metabolic energy,and nitrogen utilization were higher in the FRSD and CSD groups than in the RSD group(P<0.01).As compared to the RSD,FRSD feeding decreased the serum concentration of leptin but significantly increased the concentrations of immunoglobulin(Ig)A,IgG,ghrelin,and enzyme activities of amylase,lipase,and trypsin in the pancreas(P<0.05).Interestingly,the villus height,the ratio of villus height to crypt depth,and the activities of brush border enzymes(e.g.,maltase and sucrase)in the small intestine were higher in the CSD and FRSD groups than in the RSD group(P<0.05).As compared to the RSD,the FRSD feeding not only increased the expression level of the occludin in the small intestinal epithelium(P<0.05)but also elevated the expression levels of claudin-1,MUC1,and PepT1 genes in the duodenum,and elevated the expression levels of SGLT1 and CAT1 genes in the jejunum(P<0.05).Importantly,FRSD feeding significantly decreased the abundance of Escherichia coli,but increased the abundance of Lactobacillus and the content of butyrate in the cecum and colon(P<0.05).These results indicated that compared with rapeseed meal,fermented rapeseed meal exhibited a positive effect on improving the growth performance and intestinal health in growing pigs,and the results may also help develop novel protein sources for animal nutrition and the feed industry.
基金Supported by"863"Project of Ministry of Science and Technology of China(2013AA102504-03)
文摘Methionine (Met) and lysine (Lys) have been reported as the first two limiting amino acids (AA) for maximum milk yield and milk protein production. Supplying these AA may improve microbial protein synthesis and therefore improve milk production without adding excess N to the environment. This observation utilized fermented soybean meal (SBM), cottonseed meal (CSM), rapeseed meal (RSM) and corn by Bacillus subtilis 168 and Leuconostoc mesenteroides as core feedstuffs to produce special biological protein feed for dairy cow. The results showed that the milk production, milk protein percentage, milk fat percentage and milk DM percentage of test groups in trial period were significantly more than those of the control group (P〈0.01), the results showed that adding fermenting protein feed in dairy cow diets could significantly improve milk yield, milk protein and milk fat content. The economic benefits of actual application were analyzed, the group of 0.5% was the best compared with the other groups.