Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIPI gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-vi...Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIPI gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene ( reporter gene) and p21WAF-1/CIPI gee (therapeutic gene) using the GE7 system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/ CIPI gene in transfected U251MG cell was examined by immunohistochemis-try staining. Results: The highest transfer rate of exogenous gene was 70% . After transfection with p21WAF-1/CIPI gene, the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21WAF-1/CIPI gene can induce apoptosis of glioma cell and inhibit its growth.展开更多
Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus ...Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus Kuala Lumpur.Methods:Twenty first instar Ae.aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried-leaf powder as their source of food.Combination of TMOF-Bti in rice husk formulation with the following weights viz 10,25,50 and 100 mg,respectively in duplicate was distributed in the buckets;while TMOF-Bti in wettable powder formulation each weighing viz 2,5,10 and 20 mg,respectively in duplicate was also placed in the buckets.The control buckets run in duplicate with 4 L of water and 20 first instar Ae.aegypti larvae.All buckets were covered with mosquito netting.Larval mortality was recorded after 24 hours and weekly for five weeks.A new batch of 20 1^(st) instar larvae Ae.aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder.The experiment was repeated for four times.Results:The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied.The TMOF(2%) + Bti(2%) had a good residual effect until the end of 3^(rd) week,TMOF(4%) + Bti(4%) until 4^(th) week,wettable powder TMOF(20%) + Bti(20%) until the third week.Condusions:From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.展开更多
The cross-dimensional dynamical systems have received increasing research attention in recent years.This paper characterizes the structure features of the cross-dimensional vector space.Specifically,it is proved that ...The cross-dimensional dynamical systems have received increasing research attention in recent years.This paper characterizes the structure features of the cross-dimensional vector space.Specifically,it is proved that the completion of cross-dimensional vector space is an infinite-dimensional separable Hilbert space.Hence,it means that one can isometrically and linearly embed the crossdimensional vector space into theℓ^(2),which is known as the space of square summable sequences.This result will be helpful in the modeling and analyzing the dynamics of cross-dimensional dynamical systems.展开更多
Mounting evidence has demonstrated that CD4^(+)T cells play an important role in anti-tumor immune responses.Thus,adoptive transfer of these cells may have great potential for anti-cancer therapy.However,due to the di...Mounting evidence has demonstrated that CD4^(+)T cells play an important role in anti-tumor immune responses.Thus,adoptive transfer of these cells may have great potential for anti-cancer therapy.However,due to the difficulty to generate sufficient tumor-specific CD4^(+)T cells,the use of CD4^(+)T cells in tumor therapy is limited.It has been found that IL-15 transfection enhances the proliferation and anti-tumor activity of tumor-specific CD8+Tcells,but the effect of IL-15 transfection on CD4^(+)T cells remains unknown.Here,the effects of retrovirusmediated IL-15 expression in Ova-specific CD4^(+)T cells from Do11.10 mice were evaluated and it was discovered that IL-15 transfected CD4^(+)T cells expressed both soluble and membrane-bound IL-15.Retrovirusmediated IL-15 expression led to a selective expansion of antigen-specific CD4^(+)T cells by inhibiting their apoptosis.In vivo IL-15 transfected CD4^(+)T cells were more effective in suppressing tumor growth than control retroviral vector transfected ones.To ensure the safety of the method,the employment of thymidine kinase gene made it possible to eliminate these transgenic CD4^(+)T cells following ganciclovir treatment.Together,we show that IL-15 transfection induced a selective expansion of antigen-specific CD4^(+)T cells ex vivo and enhanced their tumor-suppression effects in vivo.This has an important significance for improving the efficacy of adoptive T cell therapy.展开更多
Hematopoietic stem cells(HSCs)are an attractive target for gene therapy,especially for inherited blood diseases.Moreover,recombinant lentiviral vectors are considered to be prospective in HSCs gene therapy for the hig...Hematopoietic stem cells(HSCs)are an attractive target for gene therapy,especially for inherited blood diseases.Moreover,recombinant lentiviral vectors are considered to be prospective in HSCs gene therapy for the high efficiency of infection.In this study,murine mononuclear cells(MNCs)were isolated from bone marrow and cultured in suspension,and then Lin−CD117+HSCs were isolated by immunomagnetic beads.During culturing,cells and colonies increased in HSCs supplied with cytokines while no change was observed in the control group without cytokines.FUXW recombinant lentiviral vectors were produced by calcium phosphate-mediated transient cotransfection infected MNCs from ICR and C57 mice.The hFIX expressions were 41.7±4.2 ng/mL and 34.5±6.6 ng/mL in supernatant on 7d.The hFIX expressions of HSCs infected by FUXW recombinant lentiviral vectors were 46.6±5.7 ng/mL(with cytokines)and 33.3±4.8 ng/mL(without cytokines)in supernatant on 7d.Results indicate that recombinant lentiviral vectors can infect murine MNCs and Lin−CD117+HSCs efficiently,and expression of the transgene can be improved when supplied with cytokines.展开更多
In this paper, we study the integral solution operators for the -equations on pseudoconvex domains. As a generalization of [1] for the -dequations on pseudoconvex domains with boundary of class C∞, we obtain the ex...In this paper, we study the integral solution operators for the -equations on pseudoconvex domains. As a generalization of [1] for the -dequations on pseudoconvex domains with boundary of class C∞, we obtain the explicit integral operator solutions of C -form for the -equations on pseudoconvex open sets with boundary of Ck (k≥0) and the sup-norm estimates of which solutions have similar as that [1] in form.展开更多
Norepinephrine transporter （NET） transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine （^131I-MIBG） in non-neuroendocrine tumors. However, the use of ^131I-MIBG is limited by its s...Norepinephrine transporter （NET） transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine （^131I-MIBG） in non-neuroendocrine tumors. However, the use of ^131I-MIBG is limited by its short retention time in target cells. To prolong the retention of ^131I-MIBG in target cells, we infected hepatocarcinoma （HepG2） cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 （VMAT2） genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of 131I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET- expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher ^131I-MIBG uptake than controls. Two hours after removal of ^131I-MIBG-containing medium, 25.4% efflux was observed in NET- VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer ^131I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after ^131I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2 resulted in increased ^131I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.展开更多
基金Supported by the National High Science and Technical Foundation of China(No. 102-12-02-05)
文摘Objective: To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21WAF-1/CIPI gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-viral vector GE7 gene delivery system was constructed. The malignant human glioma cell line U251MG was transfected in vitro with β-galactosidase gene ( reporter gene) and p21WAF-1/CIPI gee (therapeutic gene) using the GE7 system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21WAF-1/ CIPI gene in transfected U251MG cell was examined by immunohistochemis-try staining. Results: The highest transfer rate of exogenous gene was 70% . After transfection with p21WAF-1/CIPI gene, the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently. FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21WAF-1/CIPI gene can induce apoptosis of glioma cell and inhibit its growth.
基金financially supported by Entogenex Industries Sdn.Bhd.,Malaysia(grant No.NN 001-2008)
文摘Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus Kuala Lumpur.Methods:Twenty first instar Ae.aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried-leaf powder as their source of food.Combination of TMOF-Bti in rice husk formulation with the following weights viz 10,25,50 and 100 mg,respectively in duplicate was distributed in the buckets;while TMOF-Bti in wettable powder formulation each weighing viz 2,5,10 and 20 mg,respectively in duplicate was also placed in the buckets.The control buckets run in duplicate with 4 L of water and 20 first instar Ae.aegypti larvae.All buckets were covered with mosquito netting.Larval mortality was recorded after 24 hours and weekly for five weeks.A new batch of 20 1^(st) instar larvae Ae.aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder.The experiment was repeated for four times.Results:The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied.The TMOF(2%) + Bti(2%) had a good residual effect until the end of 3^(rd) week,TMOF(4%) + Bti(4%) until 4^(th) week,wettable powder TMOF(20%) + Bti(20%) until the third week.Condusions:From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.
基金supported by the National Natural Science Foundation of China under Grant No.61673129the Key Programs in Shaanxi Province of China under Grant No.2021JZ-12Science and the Technology Bureau Project of Yulin under Grant Nos.2019-89-2 and 2019-89-4。
文摘The cross-dimensional dynamical systems have received increasing research attention in recent years.This paper characterizes the structure features of the cross-dimensional vector space.Specifically,it is proved that the completion of cross-dimensional vector space is an infinite-dimensional separable Hilbert space.Hence,it means that one can isometrically and linearly embed the crossdimensional vector space into theℓ^(2),which is known as the space of square summable sequences.This result will be helpful in the modeling and analyzing the dynamics of cross-dimensional dynamical systems.
基金supported by the National Basic Research Program(973 Program)(No.2006CB504304)the National Programs for High Technology Research and Development Program(863 Program)(No.2006AA02Z4B9).
文摘Mounting evidence has demonstrated that CD4^(+)T cells play an important role in anti-tumor immune responses.Thus,adoptive transfer of these cells may have great potential for anti-cancer therapy.However,due to the difficulty to generate sufficient tumor-specific CD4^(+)T cells,the use of CD4^(+)T cells in tumor therapy is limited.It has been found that IL-15 transfection enhances the proliferation and anti-tumor activity of tumor-specific CD8+Tcells,but the effect of IL-15 transfection on CD4^(+)T cells remains unknown.Here,the effects of retrovirusmediated IL-15 expression in Ova-specific CD4^(+)T cells from Do11.10 mice were evaluated and it was discovered that IL-15 transfected CD4^(+)T cells expressed both soluble and membrane-bound IL-15.Retrovirusmediated IL-15 expression led to a selective expansion of antigen-specific CD4^(+)T cells by inhibiting their apoptosis.In vivo IL-15 transfected CD4^(+)T cells were more effective in suppressing tumor growth than control retroviral vector transfected ones.To ensure the safety of the method,the employment of thymidine kinase gene made it possible to eliminate these transgenic CD4^(+)T cells following ganciclovir treatment.Together,we show that IL-15 transfection induced a selective expansion of antigen-specific CD4^(+)T cells ex vivo and enhanced their tumor-suppression effects in vivo.This has an important significance for improving the efficacy of adoptive T cell therapy.
基金supported by the National Natural Science Foundation of China (No.30100102).
文摘Hematopoietic stem cells(HSCs)are an attractive target for gene therapy,especially for inherited blood diseases.Moreover,recombinant lentiviral vectors are considered to be prospective in HSCs gene therapy for the high efficiency of infection.In this study,murine mononuclear cells(MNCs)were isolated from bone marrow and cultured in suspension,and then Lin−CD117+HSCs were isolated by immunomagnetic beads.During culturing,cells and colonies increased in HSCs supplied with cytokines while no change was observed in the control group without cytokines.FUXW recombinant lentiviral vectors were produced by calcium phosphate-mediated transient cotransfection infected MNCs from ICR and C57 mice.The hFIX expressions were 41.7±4.2 ng/mL and 34.5±6.6 ng/mL in supernatant on 7d.The hFIX expressions of HSCs infected by FUXW recombinant lentiviral vectors were 46.6±5.7 ng/mL(with cytokines)and 33.3±4.8 ng/mL(without cytokines)in supernatant on 7d.Results indicate that recombinant lentiviral vectors can infect murine MNCs and Lin−CD117+HSCs efficiently,and expression of the transgene can be improved when supplied with cytokines.
文摘In this paper, we study the integral solution operators for the -equations on pseudoconvex domains. As a generalization of [1] for the -dequations on pseudoconvex domains with boundary of class C∞, we obtain the explicit integral operator solutions of C -form for the -equations on pseudoconvex open sets with boundary of Ck (k≥0) and the sup-norm estimates of which solutions have similar as that [1] in form.
基金We thank Yuanyou Yang, PhD, for helping in the preparation of ^131I- MIBG. This study was fimded by the National Natural Science Foundation of China （No. 81271602）.
文摘Norepinephrine transporter （NET） transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine （^131I-MIBG） in non-neuroendocrine tumors. However, the use of ^131I-MIBG is limited by its short retention time in target cells. To prolong the retention of ^131I-MIBG in target cells, we infected hepatocarcinoma （HepG2） cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 （VMAT2） genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of 131I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET- expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher ^131I-MIBG uptake than controls. Two hours after removal of ^131I-MIBG-containing medium, 25.4% efflux was observed in NET- VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer ^131I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after ^131I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2 resulted in increased ^131I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.
