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Magnetic resonance imaging focused on the ferritin heavy chain 1 reporter gene detects neuronal differentiation in stem cells 被引量:1
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作者 Xiao-Ya He Yi-Rui Zhou +4 位作者 Tong Mu Yi-Fan Liao Li Jiang Yong Qin Jin-Hua Cai 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第7期1563-1569,共7页
The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-t... The neuronal differentiation of mesenchymal stem cells offers a new strategy for the treatment of neurological disorders.Thus,there is a need to identify a noninvasive and sensitive in vivo imaging approach for real-time monitoring of transplanted stem cells.Our previous study confirmed that magnetic resonance imaging,with a focus on the ferritin heavy chain 1 reporter gene,could track the proliferation and differentiation of bone marrow mesenchymal stem cells that had been transduced with lentivirus carrying the ferritin heavy chain 1 reporter gene.However,we could not determine whether or when bone marrow mesenchymal stem cells had undergone neuronal differentiation based on changes in the magnetic resonance imaging signal.To solve this problem,we identified a neuron-specific enolase that can be differentially expressed before and after neuronal differentiation in stem cells.In this study,we successfully constructed a lentivirus carrying the neuron-specific enolase promoter and expressing the ferritin heavy chain 1 reporter gene;we used this lentivirus to transduce bone marrow mesenchymal stem cells.Cellular and animal studies showed that the neuron-specific enolase promoter effectively drove the expression of ferritin heavy chain 1 after neuronal differentiation of bone marrow mesenchymal stem cells;this led to intracellular accumulation of iron and corresponding changes in the magnetic resonance imaging signal.In summary,we established an innovative magnetic resonance imaging approach focused on the induction of reporter gene expression by a neuron-specific promoter.This imaging method can be used to noninvasively and sensitively detect neuronal differentiation in stem cells,which may be useful in stem cell-based therapies. 展开更多
关键词 ferric ammonium citrate ferritin heavy chain 1 gene magnetic resonance imaging mesenchymal stem cells molecular imaging neuronal differentiation neuron-like cells NEURONS neuron-specific enolase PROMOTER
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Cloning, sequencing and analyzing of the heavy chain V region genes of human polyreactive antibodies
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作者 ZHANGJINSONG MINGYEH 《Cell Research》 SCIE CAS CSCD 1994年第1期31-46,共16页
The heavy chain variable region genes of 5 human polyreactive mAbs generated in our laboratory have been cloned and sequenced using polymerase chain reaction (PCR) technique. We found that 2 and 3 mAbs utilized genes ... The heavy chain variable region genes of 5 human polyreactive mAbs generated in our laboratory have been cloned and sequenced using polymerase chain reaction (PCR) technique. We found that 2 and 3 mAbs utilized genes of the VHIV and VHIII families, respectively. The former 2 VH segments were in germline configuration. A common VH segment, with the best similarity of 90.1 % to the published VHIII germline genes, was utilized by 2 different rearranged genes encoding the V regions of other 3 mAbs. This strongly suggests that the common VH segment is a unmutated copy of an unidentified germline VHIII gene. All these polyreactive mAbs displayed a large NDN region (VH-D-JH junction). The entire H chain V regions of these polyreactive mAbs are unusually basic. The analysis of the charge properties of these mAbs as well as those of other poly- and mono- reactive mAbs from literatures prompts us to propose that the charged amino acids with a particular distribution along the H chain V region,especially the binding sites (CDRs), may be an important structural feature involved in antibody polyreactivity. 展开更多
关键词 human polyreactive antibody heavy chain variable region gene gene cloning and sequencing polymerase chain reaction (PCR)
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Rearranged Patterns of IgH and TcRγ Genes in Patients with Acute Lymphoblastic Leukemia
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作者 李守新 王辨明 李崇渔 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1997年第4期206-208,共3页
The rearrangement of immunoglobulin heavy chain gene(IgH) and T cell receptor γgene (ToRγ)was studied in 30 patients with acute lymphoblastic leukemia(ALL) by the polymerase chain reaction (PCR). 19 cases was found ... The rearrangement of immunoglobulin heavy chain gene(IgH) and T cell receptor γgene (ToRγ)was studied in 30 patients with acute lymphoblastic leukemia(ALL) by the polymerase chain reaction (PCR). 19 cases was found to have rearrangement of IgH gene,12 of TcRγ. Most of IgH rearrangement was characterized by one or two specific bands while some had more than two. Rearrangement of TcRγgene appeared as one specific band. A slight difference in number, size and lightness of bands was found among the patients. 4 different kinds of rearrangement were observed in the detection of IgH rearrangement in combination with TcRγgene. The rearranged patterns of IgH and TcRγgene as well as the clinical significance were discussed. 展开更多
关键词 acute lymphoblastic leukemia immunoglobulin heavy chain gene T cell receptor γgene polymerase chain reaction
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