疼痛是机体组织潜在或实际受损后所产生的不愉快的感觉,属于人体预警机制,而长期疼痛和过度伤害性刺激会造成机体生理功能紊乱,影响人们的正常生活,降低生活质量[1]。宽叶缬草(Valerian radix et rhizoma,VRR)为败酱科缬草属植物宽叶缬...疼痛是机体组织潜在或实际受损后所产生的不愉快的感觉,属于人体预警机制,而长期疼痛和过度伤害性刺激会造成机体生理功能紊乱,影响人们的正常生活,降低生活质量[1]。宽叶缬草(Valerian radix et rhizoma,VRR)为败酱科缬草属植物宽叶缬草Valetiana officinalis L.var.latifolia Miq.的干燥根及根茎,其性辛、苦、温,主治脘腹胀痛、风湿痹痛、腰膝酸软、失眠[2-4],民间治疗疼痛及失眠多以水煎入药主,故将宽叶缬草水提物作为研究对象。展开更多
Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of...Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of 80 adult Wistar rats were randomly selected to establish chronic MPS rat models,and randomly divided into four groups:control group,acupuncture group,internal heat acupuncture group A,and internal heat acupuncture group B,with 20 rats in each group.The rats in the control group were not given any treatment,and the rats in the acupuncture group were only given acupuncture treatment.The rats in group A were treated with internal heat acupuncture(needle heating up to 42℃),and the rats in group B were treated with internal heat acupuncture(needle heating up to 44℃).The tolerance time of plantar hot plate,the pain threshold of gastrocnemius muscle and the level of tumor necrosis factor-α(TNF-α)were observed and compared before modeling,1 d before treatment,and 1,7 and 14 d after treatment.Results:There were significant differences in hot plate tolerance time,time and interaction among the four groups(P<0.05),as well as in gastrocnemius tenderness threshold,time and interaction among the four groups(P<0.05),and there were also significant differences in TNF-αlevel among the three groups(P>0.05).There were significant differences in the number of electric shocks,time and interaction among the four groups(P<0.05).Conclusion:In contrast to conventional acupuncture treatment,internal heat acupuncture demonstrates greater efficacy in extending the tolerance duration of hot plate exposure and enhancing the pain threshold of the gastrocnemius muscle in rats afflicted with myofascial pain syndrome.Additionally,it accelerates the amelioration of inflammatory markers and motor function.However,it is important to note that the therapeutic impact of internal heat acupuncture may be influenced by its temperature,with 44℃being the most effective in this research.展开更多
目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion o...目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion of the PAG,vlPAG)注射。行为学实验采用热板法和福尔马林实验,分别以舔后爪潜伏时间(Hot-p latelatency,HPL)和疼痛(累计)评分为指标。免疫组化方法观察丙泊酚在vlPAG对福尔马林单侧足底皮下注射诱发的脊髓背角Fos蛋白表达的影响。结果行为学部分:两种疼痛模型中丙泊酚(10 g.L-1)vlPAG注射引起痛敏(P<0.01)。热板法实验中,丙泊酚vlPAG微量注射的痛敏作用可被相同部位预先注射25 mg.L-1B ic在10和20 m in时间点分别拮抗70%和71%(均P<0.01),在30和40 m in完全拮抗。在福尔马林实验中,丙泊酚vlPAG微量注射使福尔马林疼痛评分增加,此作用可被B ic(25 mg.L-1)在60 m in拮抗57%(P<0.05)。免疫组化部分中,丙泊酚vlPAG微量注射使福尔马林引起的脊髓背角各层FLI阳性细胞数明显增多(P<0.01),B ic vlPAG微量注射(25 mg.L-1)可部分拮抗丙泊酚vlPAG微量注射的作用(P<0.01)。结论在大鼠vlPAG微量注射丙泊酚能产生痛敏作用;GABAA受体部分介导了丙泊酚的以上作用。展开更多
目的观察不同剂量咪达唑仑对氯胺酮镇痛效应的影响,为临床合理配伍使用药物提供依据。方法腹腔注射25 mg/kg氯胺酮建立镇痛模型,150只小鼠均分为NS组、M组、K组、M1K组和M2K组,采用甩尾法、热板法和扭体法(n=10)分别观察腹腔注射1、2 mg...目的观察不同剂量咪达唑仑对氯胺酮镇痛效应的影响,为临床合理配伍使用药物提供依据。方法腹腔注射25 mg/kg氯胺酮建立镇痛模型,150只小鼠均分为NS组、M组、K组、M1K组和M2K组,采用甩尾法、热板法和扭体法(n=10)分别观察腹腔注射1、2 mg/kg咪达唑仑对氯胺酮镇痛小鼠甩尾潜伏期(TFL)、热板法痛阈(HPPT)和扭体次数(WTs)的影响。结果给药后5、10、15、20 min K组TEL、HPPT延长(P<0.05);各时点M组小鼠TFL、HPPT均无明显变化;M1K组和M2K组给药后10、15、20 min TFL、HPPT延长,给药后15 min M1K组和M2K组TEL、HPPT明显长于K组(P<0.05或P<0.01)。给药后15 min内K、M1K和M2K组小鼠WTs明显少于NS组(P<0.01)。但K、M1K和M2K组间差异无统计学意义(P<0.05或P<0.01)。结论一定剂量的咪达唑仑可以增强氯胺酮的体表镇痛作用。展开更多
文摘Objective:To investigate the effect of internal heat acupuncture on the tolerance time of plantar hot plate and the pain threshold of gastrocnemius in rats with chronic myofascial pain syndrome(MPS).Methods:A total of 80 adult Wistar rats were randomly selected to establish chronic MPS rat models,and randomly divided into four groups:control group,acupuncture group,internal heat acupuncture group A,and internal heat acupuncture group B,with 20 rats in each group.The rats in the control group were not given any treatment,and the rats in the acupuncture group were only given acupuncture treatment.The rats in group A were treated with internal heat acupuncture(needle heating up to 42℃),and the rats in group B were treated with internal heat acupuncture(needle heating up to 44℃).The tolerance time of plantar hot plate,the pain threshold of gastrocnemius muscle and the level of tumor necrosis factor-α(TNF-α)were observed and compared before modeling,1 d before treatment,and 1,7 and 14 d after treatment.Results:There were significant differences in hot plate tolerance time,time and interaction among the four groups(P<0.05),as well as in gastrocnemius tenderness threshold,time and interaction among the four groups(P<0.05),and there were also significant differences in TNF-αlevel among the three groups(P>0.05).There were significant differences in the number of electric shocks,time and interaction among the four groups(P<0.05).Conclusion:In contrast to conventional acupuncture treatment,internal heat acupuncture demonstrates greater efficacy in extending the tolerance duration of hot plate exposure and enhancing the pain threshold of the gastrocnemius muscle in rats afflicted with myofascial pain syndrome.Additionally,it accelerates the amelioration of inflammatory markers and motor function.However,it is important to note that the therapeutic impact of internal heat acupuncture may be influenced by its temperature,with 44℃being the most effective in this research.
文摘目的观察丙泊酚在vlPAG对大鼠伤害性感受的影响及GABAA受体在其中可能的作用。方法Sprague-Daw ley(SD)♀大鼠随机分组,丙泊酚(Propofol,Pro)和荷包牡丹碱(B icucu lline,B ic)采用中脑导水管周围灰质腹外侧区(ven-trolateral portion of the PAG,vlPAG)注射。行为学实验采用热板法和福尔马林实验,分别以舔后爪潜伏时间(Hot-p latelatency,HPL)和疼痛(累计)评分为指标。免疫组化方法观察丙泊酚在vlPAG对福尔马林单侧足底皮下注射诱发的脊髓背角Fos蛋白表达的影响。结果行为学部分:两种疼痛模型中丙泊酚(10 g.L-1)vlPAG注射引起痛敏(P<0.01)。热板法实验中,丙泊酚vlPAG微量注射的痛敏作用可被相同部位预先注射25 mg.L-1B ic在10和20 m in时间点分别拮抗70%和71%(均P<0.01),在30和40 m in完全拮抗。在福尔马林实验中,丙泊酚vlPAG微量注射使福尔马林疼痛评分增加,此作用可被B ic(25 mg.L-1)在60 m in拮抗57%(P<0.05)。免疫组化部分中,丙泊酚vlPAG微量注射使福尔马林引起的脊髓背角各层FLI阳性细胞数明显增多(P<0.01),B ic vlPAG微量注射(25 mg.L-1)可部分拮抗丙泊酚vlPAG微量注射的作用(P<0.01)。结论在大鼠vlPAG微量注射丙泊酚能产生痛敏作用;GABAA受体部分介导了丙泊酚的以上作用。
文摘目的观察不同剂量咪达唑仑对氯胺酮镇痛效应的影响,为临床合理配伍使用药物提供依据。方法腹腔注射25 mg/kg氯胺酮建立镇痛模型,150只小鼠均分为NS组、M组、K组、M1K组和M2K组,采用甩尾法、热板法和扭体法(n=10)分别观察腹腔注射1、2 mg/kg咪达唑仑对氯胺酮镇痛小鼠甩尾潜伏期(TFL)、热板法痛阈(HPPT)和扭体次数(WTs)的影响。结果给药后5、10、15、20 min K组TEL、HPPT延长(P<0.05);各时点M组小鼠TFL、HPPT均无明显变化;M1K组和M2K组给药后10、15、20 min TFL、HPPT延长,给药后15 min M1K组和M2K组TEL、HPPT明显长于K组(P<0.05或P<0.01)。给药后15 min内K、M1K和M2K组小鼠WTs明显少于NS组(P<0.01)。但K、M1K和M2K组间差异无统计学意义(P<0.05或P<0.01)。结论一定剂量的咪达唑仑可以增强氯胺酮的体表镇痛作用。
