5月27日,在2014浪潮创新数据中心全国巡展济南站,浪潮首度公布一项市场计划——IBM to Inspur(简称I2I计划),志在全面抢夺IBM X业务,加速推进服务器中国NO.1目标达成。在斯诺登事件发生之后,国内信息安全的政策、舆论环境都在向国产品...5月27日,在2014浪潮创新数据中心全国巡展济南站,浪潮首度公布一项市场计划——IBM to Inspur(简称I2I计划),志在全面抢夺IBM X业务,加速推进服务器中国NO.1目标达成。在斯诺登事件发生之后,国内信息安全的政策、舆论环境都在向国产品牌倾斜,国产化的春天已经到来。国外品牌开始在关键行业用户受到抵制,造就了国内信息产业"国进外退"的新市场格局,包括IBM、思科在内的国外品牌销量大幅下降。展开更多
M. grisea causes one of the major diseases on rice, rice blast. Appresoriumformation is the key for the pathogen to penetrate into host tissue. So, it is im-portant to study the effect of environmental factors on the ...M. grisea causes one of the major diseases on rice, rice blast. Appresoriumformation is the key for the pathogen to penetrate into host tissue. So, it is im-portant to study the effect of environmental factors on the appresorinm formation展开更多
The acute and chronic effects of sodium nitroprusside (SNP) are well characterized for vascular smooth muscle cells (VSMC). Stimulation of soluble guanylyl cyclase (sGC) gives a rapid elevation of intracellular cGMP l...The acute and chronic effects of sodium nitroprusside (SNP) are well characterized for vascular smooth muscle cells (VSMC). Stimulation of soluble guanylyl cyclase (sGC) gives a rapid elevation of intracellular cGMP levels and relaxation of VSMC. The antiproliferative effect of SNP needs days to develop. In the present study human embryonic kidney (HEK 293) cells were used to study the growth after repeated exposure to SNP. A dose-dependent antiproliferative effect was evident and after 5 days with an IC50 value of 108 μM. Cyclic GMP was able to mimic the antiproliferative effect of SNP on HEK293 cells. When cGMP (1000 μM) was added to the cell culture medium for 5 days the cell densities were reduced with 37% below baseline and cGMPin increased from 5.3 to 195 pmol/107cells. The interaction with the non-selective PDE (cyclic nucleotide phosphodiesterase) inhibitor 3-isobutyl-1-methylxanthine (IBMX) was tested after three days. IBMX alone (1000 μM) reduced cell densities with 48% and elevated cGMPin (from 5.2 to 9.3 pmol/107cells). The effect of 10 μM SNP was reinforced on proliferation (from 13% to 90%) and elevation of cGMP levels (from 7.6 to 13.5 pmol/107cells). A corresponding effect was observed after addition of 1000 μM cGMP and 1000 μM IBMX for 3 days. The antiproliferative effect of cGMP increased from 30% to 89% and the cGMPin increased from 240 to 480 pmol/107cells. However, additional mechanisms exist for the antiproliferative effect of SNP. One of these is the intracellular oxidative effect which includes production of S-nitrosoglutathione. The fall in ratios between GSH and GSSG from 260 to 85 after 100 μM SNP exposure is compatible with such a mechanism since cGMP (1000 μM) added to the culture medium did not change the ratio. This study shows that the antiproliferative effects of SNP on HEK293 cells are mediated through cGMP-dependent and cGMP-independent mechanisms. The concentration-dependent effects develop over time. HEK293 cells had an efficient efflux system for cGMP and the use of inside-out vesicles (IOVs) showed high affinity ATP-dependent cGMP transport with a Km value of 2.3 μM. The antiproliferative effect of SNP was correlated to cGMPex/in.展开更多
文摘5月27日,在2014浪潮创新数据中心全国巡展济南站,浪潮首度公布一项市场计划——IBM to Inspur(简称I2I计划),志在全面抢夺IBM X业务,加速推进服务器中国NO.1目标达成。在斯诺登事件发生之后,国内信息安全的政策、舆论环境都在向国产品牌倾斜,国产化的春天已经到来。国外品牌开始在关键行业用户受到抵制,造就了国内信息产业"国进外退"的新市场格局,包括IBM、思科在内的国外品牌销量大幅下降。
文摘M. grisea causes one of the major diseases on rice, rice blast. Appresoriumformation is the key for the pathogen to penetrate into host tissue. So, it is im-portant to study the effect of environmental factors on the appresorinm formation
文摘The acute and chronic effects of sodium nitroprusside (SNP) are well characterized for vascular smooth muscle cells (VSMC). Stimulation of soluble guanylyl cyclase (sGC) gives a rapid elevation of intracellular cGMP levels and relaxation of VSMC. The antiproliferative effect of SNP needs days to develop. In the present study human embryonic kidney (HEK 293) cells were used to study the growth after repeated exposure to SNP. A dose-dependent antiproliferative effect was evident and after 5 days with an IC50 value of 108 μM. Cyclic GMP was able to mimic the antiproliferative effect of SNP on HEK293 cells. When cGMP (1000 μM) was added to the cell culture medium for 5 days the cell densities were reduced with 37% below baseline and cGMPin increased from 5.3 to 195 pmol/107cells. The interaction with the non-selective PDE (cyclic nucleotide phosphodiesterase) inhibitor 3-isobutyl-1-methylxanthine (IBMX) was tested after three days. IBMX alone (1000 μM) reduced cell densities with 48% and elevated cGMPin (from 5.2 to 9.3 pmol/107cells). The effect of 10 μM SNP was reinforced on proliferation (from 13% to 90%) and elevation of cGMP levels (from 7.6 to 13.5 pmol/107cells). A corresponding effect was observed after addition of 1000 μM cGMP and 1000 μM IBMX for 3 days. The antiproliferative effect of cGMP increased from 30% to 89% and the cGMPin increased from 240 to 480 pmol/107cells. However, additional mechanisms exist for the antiproliferative effect of SNP. One of these is the intracellular oxidative effect which includes production of S-nitrosoglutathione. The fall in ratios between GSH and GSSG from 260 to 85 after 100 μM SNP exposure is compatible with such a mechanism since cGMP (1000 μM) added to the culture medium did not change the ratio. This study shows that the antiproliferative effects of SNP on HEK293 cells are mediated through cGMP-dependent and cGMP-independent mechanisms. The concentration-dependent effects develop over time. HEK293 cells had an efficient efflux system for cGMP and the use of inside-out vesicles (IOVs) showed high affinity ATP-dependent cGMP transport with a Km value of 2.3 μM. The antiproliferative effect of SNP was correlated to cGMPex/in.
