采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS...采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。展开更多
Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island, Wenzhou, Zhejiang Province, China. Antibacterial screening using the agar disc method showed that 19 of ...Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island, Wenzhou, Zhejiang Province, China. Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea. Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio. Strain NJ0104 had the widest antimicrobial spectrum and strong activity, particularly against Fibrio parahaemolyticus-MM0810072. A preliminary study of N J0104 antibacterial metabolites demonstrated that they had thermal stability up to 80℃, ultraviolet stability up to 40 min and pH stability between 4.0-7.0. In addition, the antibacterial metabolites were readily soluble in butanol. To identify the specific strain, the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced. Based on the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp.展开更多
采用比浊法对本试验室保存的126株南极微生物产适冷溶菌酶进行测定,发现7株菌产溶菌酶。并对其中的菌株NJ147进行ITS-5.8 S rDNA基因序列的同源性和系统发育分析,结果表明:菌株NJ147属于Debaryomyces hansenii属。该菌株所分泌溶菌酶的...采用比浊法对本试验室保存的126株南极微生物产适冷溶菌酶进行测定,发现7株菌产溶菌酶。并对其中的菌株NJ147进行ITS-5.8 S rDNA基因序列的同源性和系统发育分析,结果表明:菌株NJ147属于Debaryomyces hansenii属。该菌株所分泌溶菌酶的最适作用温度为30℃,在0℃时酶活性是最高活性的32.6%;对热敏感,其半衰期为40℃保温30 min;最适pH为6.0,是一种典型的适冷酶。该酶对6种受试菌株均有不同程度的抑制,具有广谱杀菌谱,具有潜在的工业应用价值。展开更多
文摘采用通用引物PCR扩增法,测定了辽东湾海域的白色霞水母(Cyanea nozakii)螅状体、碟状体及水母体的18S以及ITS-5.8S r DNA序列,同时利用Gene Bank数据库中已有同源序列对其进行序列分析及系统分析。结果显示,白色霞水母3个个体的18S和ITS-5.8S r DNA序列完全一致。白色霞水母样品的ITS-5.8S r DNA序列与Gen Bank中未知真核生物的序列高度相似(≥99%),推测该物种可能是早期发育阶段(卵、浮浪幼虫或碟状体)的白色霞水母样品。霞水母属不同种间18S r DNA序列经比对后同源序列长度为1709bp,多态位点数33个;比对后ITS1同源序列长度为368bp,其中变异位点203个,简约信息位点数178个,单变异位点21个。基于18S r DNA基因序列的霞水母属种内和种间平均遗传距离分别为0、0.008,而基于ITS1序列的霞水母属种内和种间平均遗传距离分别为0.019、0.284。基于ITS1的种间遗传距离是种内遗传距离的15倍,适合于进行物种鉴定。NJ系统树的结果也表明同种的不同个体各自聚枝,其聚类结果大致与形态分类吻合。研究表明,ITS基因片段在霞水母不同种间变异较大,更适于大型水母种类鉴定、检测及属内种间水平的系统进化研究。
基金Supported by the General Program of Zhejiang Science and Technology Department(No.2009C33004)
文摘Seventy-eight marine fungal strains were isolated from sediment samples collected off the coast of Nanji Island, Wenzhou, Zhejiang Province, China. Antibacterial screening using the agar disc method showed that 19 of the isolated strains could inhibit at least one pathogenic Vibrio from Pseudosciaena crocea. Subsequent screening confirmed that nine strains produced antibacterial metabolites that had activity against one or several types of pathogenic Vibrio. Strain NJ0104 had the widest antimicrobial spectrum and strong activity, particularly against Fibrio parahaemolyticus-MM0810072. A preliminary study of N J0104 antibacterial metabolites demonstrated that they had thermal stability up to 80℃, ultraviolet stability up to 40 min and pH stability between 4.0-7.0. In addition, the antibacterial metabolites were readily soluble in butanol. To identify the specific strain, the ITS-5.8S rDNA regions of NJ0104 were PCR amplified and sequenced. Based on the combination of phenotypic and genotypic data, the strain was identified as Arthrinium sp.
文摘采用比浊法对本试验室保存的126株南极微生物产适冷溶菌酶进行测定,发现7株菌产溶菌酶。并对其中的菌株NJ147进行ITS-5.8 S rDNA基因序列的同源性和系统发育分析,结果表明:菌株NJ147属于Debaryomyces hansenii属。该菌株所分泌溶菌酶的最适作用温度为30℃,在0℃时酶活性是最高活性的32.6%;对热敏感,其半衰期为40℃保温30 min;最适pH为6.0,是一种典型的适冷酶。该酶对6种受试菌株均有不同程度的抑制,具有广谱杀菌谱,具有潜在的工业应用价值。
