Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated a...Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.展开更多
The Du-Huo-Ji-Sheng-Tang(DHJST)is the top commercial concentrated aqueous extract used in clinics for treating osteoarthritis.However,choosing the quality control markers for DHJST is an important problem for herbal m...The Du-Huo-Ji-Sheng-Tang(DHJST)is the top commercial concentrated aqueous extract used in clinics for treating osteoarthritis.However,choosing the quality control markers for DHJST is an important problem for herbal medicine industry.In this study,DHJST was divided into three groups according to the medical philosophy of traditional Chinese medicine(TCM),such as Si-Wu-Tang(SWT),wind-dampness herbs(WDH)and tonifying展开更多
On August 22,the reporter learned from MLR’s Press Conference for Identified Reserves of Mineral Resources of China in2016 that,China’s investment in mining fixed assets and geological exploration had declined for f...On August 22,the reporter learned from MLR’s Press Conference for Identified Reserves of Mineral Resources of China in2016 that,China’s investment in mining fixed assets and geological exploration had declined for four consecutive years by 2016,and resource reserves growth rate had slowed down due to other reasons such as downturn展开更多
Nowadays,wood identification is made by experts using hand lenses,wood atlases,and field manuals which take a lot of cost and time for the training process.The quantity and species must be strictly set up,and accurate...Nowadays,wood identification is made by experts using hand lenses,wood atlases,and field manuals which take a lot of cost and time for the training process.The quantity and species must be strictly set up,and accurate identification of the wood species must be made during exploitation to monitor trade and enforce regulations to stop illegal logging.With the development of science,wood identification should be supported with technology to enhance the perception of fairness of trade.An automatic wood identification system and a dataset of 50 commercial wood species from Asia are established,namely,wood anatomical images collected and used to train for the proposed model.In the convolutional neural network(CNN),the last layers are usually soft-max functions with dense layers.These layers contain the most parameters that affect the speed model.To reduce the number of parameters in the last layers of the CNN model and enhance the accuracy,the structure of the model should be optimized and developed.Therefore,a hybrid of convolutional neural network and random forest model(CNN-RF model)is introduced to wood identification.The accuracy’s hybrid model is more than 98%,and the processing speed is 3 times higher than the CNN model.The highest accuracy is 1.00 in some species,and the lowest is 0.92.These results show the excellent adaptability of the hybrid model in wood identification based on anatomical images.It also facilitates further investigations of wood cells and has implications for wood science.展开更多
The structure of bupirimate was identified by using MS and NMR.The structures of 10 impurities were identified by using GC-MS.The method provides a powerful tool for structure analysis special in complex samples.
In each equation of simultaneous Equation model, the exogenous variables need to satisfy all the basic assumptions of linear regression model and be non-negative especially in econometric studies. This study examines ...In each equation of simultaneous Equation model, the exogenous variables need to satisfy all the basic assumptions of linear regression model and be non-negative especially in econometric studies. This study examines the performances of the Ordinary Least Square (OLS), Two Stage Least Square (2SLS), Three Stage Least Square (3SLS) and Full Information Maximum Likelihood (FIML) Estimators of simultaneous equation model with both normally and uniformly distributed exogenous variables under different identification status of simultaneous equation model when there is no correlation of any form in the model. Four structural equation models were formed such that the first and third are exact identified while the second and fourth are over identified equations. Monte Carlo experiments conducted 5000 times at different levels of sample size (n = 10, 20, 30, 50, 100, 250 and 500) were used as criteria to compare the estimators. Result shows that OLS estimator is best in the exact identified equation except with normally distributed exogenous variables when . At these instances, 2SLS estimator is best. In over identified equations, the 2SLS estimator is best except with normally distributed exogenous variables when the sample size is small and large, and;and with uniformly distributed exogenous variables when n is very large, , the best estimator is either OLS or FIML or 3SLS.展开更多
DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制...DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制定了DOI国际标准。DOI像文献的身份证一样,具有唯一性和永久性,帮助出版社、作者保护知识产权,解决了死链等问题。多个国际大型出版商Elsevier、Blackwell、Wiley、Springer等都使用DOI对数字资源进行标识。展开更多
DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制...DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制定了DOI国际标准。DOI像文献的身份证一样,具有唯一性和永久性,帮助出版社、作者保护知识产权,解决了死链等问题。多个国际大型出版商Elsevier、Blackwell、Wiley、Springer等都使用DOI对数字资源进行标识。各出版商、杂志社、期刊可通过DOI代理机构完成DOI申请和注册。展开更多
Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles...Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.展开更多
With the help of a phenomenological approach outlined in the text in some detail, we have dealt here with the description of the plots on rapidity and pseudorapidity spectra of some hadron-secondaries produced in vari...With the help of a phenomenological approach outlined in the text in some detail, we have dealt here with the description of the plots on rapidity and pseudorapidity spectra of some hadron-secondaries produced in various nucleus-nucleus interactions at high energies. The agreement between the measured data and the attempted fits are, on the whole, modestly satisfactory excepting a very narrow central region in the vicinity of y = η = 0. At last, hints to how the steps suggested in the main body of the text to proceed with the description of the measured data given in the plots could lead finally to a somewhat systematic methodology have also been made.展开更多
That the values of average transverse momenta ( ) of the secondaries produced in high energy collisions rise very slowly with energy is modestly well-known and accepted. We would like to probe into this aspect of the ...That the values of average transverse momenta ( ) of the secondaries produced in high energy collisions rise very slowly with energy is modestly well-known and accepted. We would like to probe into this aspect of the problem for production of the main variety of the 'soft' secondaries in two high energy symmetric nuclear collisions with the help of two non-QCD models. Our model-based results are found to be quite consistent with the anticipated behaviours and also with the observations.展开更多
文摘Studying on the genetic diversity and genetic relationship of flowering cherry cultivars is extremely important for germplasm conservation, cultivar identification and breeding. Flowering cherry is widely cultivated as an important woody ornamental plant in worldwide, especially Japan, China. However, owning to the morphological similarity, many cultivars are distinguished hardly in non-flowering season. Here, we evaluated the genetic diversity and genetic relationship of 40 flowering cherry cultivars, which are mainly cultivated in China. We selected 13 polymorphicprimers to amplify to allele fragments with fluorescent-labeled capillary electrophoresis technology. The population structure analysis results show that these cultivars could be divided into 4 subpopulations. At the population level, N<sub>a</sub> and N<sub>e</sub> were 6.062, 4.326, respectively. H<sub>o</sub> and H<sub>e</sub> were 0.458 and 0.670, respectively. The Shannon’s information index (I) was 1.417. The Pop3, which originated from P. serrulata, had the highest H<sub>o</sub>, H<sub>e</sub>, and I among the 4 subpopulations. AMOVA showed that only 4% of genetic variation came from populations, the 39% variation came from individuals and 57% (p < 0.05) came from intra-individuals. 5 polymorphic SSR primers were selected to construct molecular ID code system of these cultivars. This analysis on the genetic diversity and relationship of the 40 flowering cherry cultivars will help to insight into the genetic background, relationship of these flowering cherry cultivars and promote to identify similar cultivars.
文摘The Du-Huo-Ji-Sheng-Tang(DHJST)is the top commercial concentrated aqueous extract used in clinics for treating osteoarthritis.However,choosing the quality control markers for DHJST is an important problem for herbal medicine industry.In this study,DHJST was divided into three groups according to the medical philosophy of traditional Chinese medicine(TCM),such as Si-Wu-Tang(SWT),wind-dampness herbs(WDH)and tonifying
文摘On August 22,the reporter learned from MLR’s Press Conference for Identified Reserves of Mineral Resources of China in2016 that,China’s investment in mining fixed assets and geological exploration had declined for four consecutive years by 2016,and resource reserves growth rate had slowed down due to other reasons such as downturn
文摘Nowadays,wood identification is made by experts using hand lenses,wood atlases,and field manuals which take a lot of cost and time for the training process.The quantity and species must be strictly set up,and accurate identification of the wood species must be made during exploitation to monitor trade and enforce regulations to stop illegal logging.With the development of science,wood identification should be supported with technology to enhance the perception of fairness of trade.An automatic wood identification system and a dataset of 50 commercial wood species from Asia are established,namely,wood anatomical images collected and used to train for the proposed model.In the convolutional neural network(CNN),the last layers are usually soft-max functions with dense layers.These layers contain the most parameters that affect the speed model.To reduce the number of parameters in the last layers of the CNN model and enhance the accuracy,the structure of the model should be optimized and developed.Therefore,a hybrid of convolutional neural network and random forest model(CNN-RF model)is introduced to wood identification.The accuracy’s hybrid model is more than 98%,and the processing speed is 3 times higher than the CNN model.The highest accuracy is 1.00 in some species,and the lowest is 0.92.These results show the excellent adaptability of the hybrid model in wood identification based on anatomical images.It also facilitates further investigations of wood cells and has implications for wood science.
文摘The structure of bupirimate was identified by using MS and NMR.The structures of 10 impurities were identified by using GC-MS.The method provides a powerful tool for structure analysis special in complex samples.
文摘In each equation of simultaneous Equation model, the exogenous variables need to satisfy all the basic assumptions of linear regression model and be non-negative especially in econometric studies. This study examines the performances of the Ordinary Least Square (OLS), Two Stage Least Square (2SLS), Three Stage Least Square (3SLS) and Full Information Maximum Likelihood (FIML) Estimators of simultaneous equation model with both normally and uniformly distributed exogenous variables under different identification status of simultaneous equation model when there is no correlation of any form in the model. Four structural equation models were formed such that the first and third are exact identified while the second and fourth are over identified equations. Monte Carlo experiments conducted 5000 times at different levels of sample size (n = 10, 20, 30, 50, 100, 250 and 500) were used as criteria to compare the estimators. Result shows that OLS estimator is best in the exact identified equation except with normally distributed exogenous variables when . At these instances, 2SLS estimator is best. In over identified equations, the 2SLS estimator is best except with normally distributed exogenous variables when the sample size is small and large, and;and with uniformly distributed exogenous variables when n is very large, , the best estimator is either OLS or FIML or 3SLS.
文摘DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制定了DOI国际标准。DOI像文献的身份证一样,具有唯一性和永久性,帮助出版社、作者保护知识产权,解决了死链等问题。多个国际大型出版商Elsevier、Blackwell、Wiley、Springer等都使用DOI对数字资源进行标识。
文摘DOI全称数字对象唯一标识符(digital object unique identifier)。1998年美国出版协会(the Association of America Publishers,AAP)创立非营利性组织国际DOI基金会(international DOI foundation,http://www.doi.org),建立并制定了DOI国际标准。DOI像文献的身份证一样,具有唯一性和永久性,帮助出版社、作者保护知识产权,解决了死链等问题。多个国际大型出版商Elsevier、Blackwell、Wiley、Springer等都使用DOI对数字资源进行标识。各出版商、杂志社、期刊可通过DOI代理机构完成DOI申请和注册。
文摘Peculiar properties of morphological structures of organelle membranes were studied by fluorescent confocal microscopy. The list of objects in our experiments was represented by mitochondria, chloroplasts and vacuoles. During this study, identification of lipid microinclusions having the form of such lipid-protein structural microformations as lipid-protein microdomains, vesicles and membrane tubular structures (cytoplasmic transvacuolar strands and nanotubes) located in organelle membranes or bound up with them was conducted. Such membrane probes as laurdan, DPH, ANS and bis-ANS were used. Comparison of fluorescence intensity of these membrane probes was conducted. This investigation of the morphological properties of lipid-protein structural microformations was accompanied with analysis of 1) the phase state and 2) dynamics of microviscosity variations in the membrane elements of isolated plant cell organelles. Distributions of laurdan fluorescence generalized polarization (GP) values for the membrane on the whole and for the intensively fluorescing membrane segments were obtained. It was discovered that the microviscosity of intensively fluorescing membrane segments essentially differed from the microviscosity of the rest part of the membrane. In conclusion, some results of the study of peculiar properties of lipid-protein structural microformations related to the structure of organelle membranes and the discoveries made in this investigation are discussed.
文摘With the help of a phenomenological approach outlined in the text in some detail, we have dealt here with the description of the plots on rapidity and pseudorapidity spectra of some hadron-secondaries produced in various nucleus-nucleus interactions at high energies. The agreement between the measured data and the attempted fits are, on the whole, modestly satisfactory excepting a very narrow central region in the vicinity of y = η = 0. At last, hints to how the steps suggested in the main body of the text to proceed with the description of the measured data given in the plots could lead finally to a somewhat systematic methodology have also been made.
文摘That the values of average transverse momenta ( ) of the secondaries produced in high energy collisions rise very slowly with energy is modestly well-known and accepted. We would like to probe into this aspect of the problem for production of the main variety of the 'soft' secondaries in two high energy symmetric nuclear collisions with the help of two non-QCD models. Our model-based results are found to be quite consistent with the anticipated behaviours and also with the observations.