[Objective] The paper was to establish an affordable indoor virus inoculation and identification technology. [Method] Taking Tobacco Mosaic Virus （TMV） and Cucumber Mosaic Vires （CMV） of sweet （hot） pepper as th...[Objective] The paper was to establish an affordable indoor virus inoculation and identification technology. [Method] Taking Tobacco Mosaic Virus （TMV） and Cucumber Mosaic Vires （CMV） of sweet （hot） pepper as the sources of virus, an affordable indoor virus inoculation and identification technology was developed in the paper. [ Result] The suitable inoculation concentration of CMV was five to ten times, and the best seedling age for inoculation was five to six leav- es. Suitable inoculation concentration of TMV was 20 to 30 times, and the best seedling age for inoculation was three to six leaves. Single inoculation technology was mainly used for indoor virus inoculation and identification of sweet （hot） pepper, and complex inoculation technology could also be adopted with first, st inoculation of CMV and late inoculation of TMV. For mixed inoculation technology, CMV： TMV should be 1： 1. Complex inoculation and mix inoculation should base on the tech- nology of single inoculation. Disease resistant materials, AID1-W22-dg176, ABgl-W22-48123, AB91-DL-6428, HY031-2-8-1-6, BYT-4-1-3-6-8, JFG-2-1-2-6, JF8S-1-1-5-4-8 and I＇502-1-1-3-5, were identified by this method. [ Conclusion] This research provided scientific basis for standardization of indoor inoculation of sweet （hot） pepper virus.展开更多
基金Supported by National Science and Technology Support Program(2012BAD02B02-10)Shijiazhuang Experiment Station of National Staple Vegetable Industrial Technology System(CARS-25-G-05)+2 种基金Science and Technology Support Program of Hebei Province(11220102D-1)Project of Hebei Science and Technology Hall(11220701D)Youth Foundation of Hebei Academy of Agriculture and Forestry Sciences(A2012050302)
文摘[Objective] The paper was to establish an affordable indoor virus inoculation and identification technology. [Method] Taking Tobacco Mosaic Virus （TMV） and Cucumber Mosaic Vires （CMV） of sweet （hot） pepper as the sources of virus, an affordable indoor virus inoculation and identification technology was developed in the paper. [ Result] The suitable inoculation concentration of CMV was five to ten times, and the best seedling age for inoculation was five to six leav- es. Suitable inoculation concentration of TMV was 20 to 30 times, and the best seedling age for inoculation was three to six leaves. Single inoculation technology was mainly used for indoor virus inoculation and identification of sweet （hot） pepper, and complex inoculation technology could also be adopted with first, st inoculation of CMV and late inoculation of TMV. For mixed inoculation technology, CMV： TMV should be 1： 1. Complex inoculation and mix inoculation should base on the tech- nology of single inoculation. Disease resistant materials, AID1-W22-dg176, ABgl-W22-48123, AB91-DL-6428, HY031-2-8-1-6, BYT-4-1-3-6-8, JFG-2-1-2-6, JF8S-1-1-5-4-8 and I＇502-1-1-3-5, were identified by this method. [ Conclusion] This research provided scientific basis for standardization of indoor inoculation of sweet （hot） pepper virus.
