The study on the changes of stomatal sensitivity in relation to xylem ABA during periodical soil drying and the effect of leaf water status on the stomatal sensitivity has confirmed that xylem ABA concentration is a g...The study on the changes of stomatal sensitivity in relation to xylem ABA during periodical soil drying and the effect of leaf water status on the stomatal sensitivity has confirmed that xylem ABA concentration is a good indicator of soil water status around roots and the relation between xylem ABA concentration and predawn leaf water potential remained constant during the three consecutive soil drying cycles based on the slopes of the fitted lines. The sensitivity of stomata to xylem ABA increased substantially as the soil drying cycles progressed, and the xylem ABA concentration needed to cause a 50% decrease of stomatal conductance was as low as 550 nmol/L in the next two soil drying cycle, as compared with the 750 nmol/L ABA in the first cycle of soil drying. The results using the split_root system showed that leaf water deficit significantly enhanced the stomatal response to xylem ABA and the xylem ABA concentration needed to cause a 50% decrease in stomatal conductance was 2 to 4 times smaller in the whole_root_drying treatment than those in the semi_root_drying treatment. These results suggested that the sensitivity of stomata to xylem ABA concentration is not a fixed characteristic.展开更多
The water deficit in arid and semi-arid regions is the primary limiting factor for the development of urban greenery and forestation. In addition, planting the species that consume low levels of water is useful in ari...The water deficit in arid and semi-arid regions is the primary limiting factor for the development of urban greenery and forestation. In addition, planting the species that consume low levels of water is useful in arid and semi-arid regions that have poor water management measures. Leaf water potential(Ψ) is a physiological parameter that can be used to identify drought resistance in various species. Indeed, Ψ is one of the most important properties of a plant that can be measured using a pressure chamber. Drought avoiding or drought resistant species have a lower Ψ than plants that use normal or high levels of water. To determine drought resistance of species that are suitable for afforestation in arid urban regions, we evaluated twenty woody species in the Isfahan City, central Iran. The experimental design was random split-split plots with five replications. The species were planted outdoor in plastic pots and then subjected to treatments that consisted of two soil types and five drip irrigation regimes. To evaluate the resistance of each species to drought, we used the Ψ and the number of survived plants to obtain the drought resistance index(DRI). Then, cluster analysis, dendrogram, and similarity index were used to group the species using DRI. Result indicates that the evaluated species were classified into five groups:(1) high water consuming species(DRI>–60 MPa);(2) above normal water consuming species(–60 MPa≥DRI>–90 MPa);(3) normal water consuming species(–90 MPa≥DRI>–120 MPa);(4) semi-drought resistant species(–120 MPa≥DRI>–150 MPa);and(5) drought resistant species(DRI≤–150 MPa). According to the DRI, Salix babylonica L., Populus alba L., and P. nigra L. are high water consuming species, Platanus orientalis L. and Albizia julibrissin Benth are normal water consuming species, and Quercus infectoria Oliv. and Olea europaea L. can be considered as drought resistant species.展开更多
Regulation of leaf gas exchange plays an important role in the survival of trees and shrubs under future climate change. However, the responses of leaf water potential and gas exchange of shrubs in semi-arid areas to ...Regulation of leaf gas exchange plays an important role in the survival of trees and shrubs under future climate change. However, the responses of leaf water potential and gas exchange of shrubs in semi-arid areas to the precipitation alteration are not clear. Here, we conducted a manipulated experiment with three levels of precipitation, i.e., a control with ambient precipitation, 50% above ambient precipitation(irrigation treatment), and 50% below ambient precipitation(drought treatment), with two common shrubs, Salix psammophila C. Wang & C. Y. Yang(isohydric plant, maintaining a constant leaf water potential by stomatal regulation) and Caragana korshinskii Kom.(anisohydric plant, having more variable leaf water potential), on the Chinese Loess Plateau in 2014 and 2015. We measured the seasonal variations of predawn and midday leaf water potential(Ψpd and Ψmd), two parameters of gas exchange, i.e., light-saturated assimilation(An) and stomatal conductance(gs), and other foliar and canopy traits. The isohydric S. psammophila had a similar An and a higher gs than the anisohydric C. korshinskii under drought treatment in 2015, inconsistent with the view that photosynthetic capacity of anisohydric plants is higher than isohydric plants under severe drought. The two shrubs differently responded to precipitation manipulation. Ψpd, An and gs were higher under irrigation treatment than control for S. psammophila, and these three variables and Ψmd were significantly higher under irrigation treatment and lower under drought treatment than control for C. korshinskii. Leaf water potential and gas exchange responded to manipulated precipitation more strongly for C. korshinskii than for S. psammophila. However, precipitation manipulation did not alter the sensitivity of leaf gas exchange to vapor-pressure deficit and soil moisture in these two shrubs. Acclimation to long-term changes in soil moisture in these two shrubs was primarily attributed to the changes in leaf or canopy structure rather than leaf gas exchange. These findings will be useful for modeling canopy water-carbon exchange and elucidating the adaptive strategies of these two shrubs to future changes in precipitation.展开更多
Seedlings of drought-tolerance rice varieties Han 501and Han A03,and the drought sensitive varietiesNanjing 11 and Yanjing 2 were raised in a paddyfield and transplanted into pots at the age of 8leaves.Water stress st...Seedlings of drought-tolerance rice varieties Han 501and Han A03,and the drought sensitive varietiesNanjing 11 and Yanjing 2 were raised in a paddyfield and transplanted into pots at the age of 8leaves.Water stress started at the tillering stage byholding water from 0 MPa of the soil water potentialin pots till the leaves showed seriously wilting.展开更多
Differences in transpiration and leaf water potential （LWP） in relation to cassava yield were investigated along inland valley toposequence in a 4×4 Latin square design. The landrace with the highest transpirat...Differences in transpiration and leaf water potential （LWP） in relation to cassava yield were investigated along inland valley toposequence in a 4×4 Latin square design. The landrace with the highest transpiration rate and lowest LWP yielded the lowest, while TMS 91/02324 and TMS 91/02327 with intermediate rate and highest LWP yielded the highest, indicating that high transpiration rate associated with low LWP reduced yield. Transpiration was lower in the fringe with deeper water table than valley bottom at deep water table site, while at shallow water table, it was higher in the fringe than valley bottom, suggesting that drought and excess moisture reduced transpiration. LWP and water table depth correlated negatively indicating that shallow water table reduced transpiration by reducing LWP. Transpiration increased and LWP decreased as radiation, leaf temperature and vapour pressure deficit increased and differences in these microclimatic conditions caused differences in the two processes between sites, years and time of day. Under mild water stress, transpiration and LWP were higher in the afternoon than the morning, but the reverse occurred under severe stress. TMS 91/02324 and TMS 91/02327 had the highest LWP under severe stress, indicating their higher drought tolerance than the other cultivars.展开更多
A greenhouse pot experiment was conducted using a complete random design with six replications. A pressure-volume curve analysis was employed to study the effects of organic fertilizers on plant water relations in swe...A greenhouse pot experiment was conducted using a complete random design with six replications. A pressure-volume curve analysis was employed to study the effects of organic fertilizers on plant water relations in sweet corn (Zea mays L. cv. Honey-bantam) in terms of leaf osmotic concentration (Cs), osmotic potentials at full turgid status (πFT) and at incipient plasmolysis (πIP), and of symplastic (ζsym) and apoplastic (ζapo) fractions in the tissue water compartment in relation to photosynthetic capacity (Pc) and dry mass accumulation. At the seedling stage (day 15), plants with chemical fertilizer treatments showed lower πFT, πIP and ζapo and higher Cs, ζsym and PC than those with organic fertilizer treatments. Compared to PC and relative growth, where values from day 15 to day 75 were first lower for organic treatments and then higher, ζsym and Cs values were similar, while πFT and πIP were opposite being higher then lower. Dry mass production with organic fertilizer was higher than or close to the chemical fertilizer treatments in the later growth stage (day 75), though dry mass production with chemical fertilizers was much higher in the early and middle growing stages (days 15 and 45). Increased photosynthesis and dry mass production in later growth stages due to organic fertilizers were associated with increased osmotic concentration in the tissue and the symplastic fraction of the tissue water compartment. These might favor stomatal opening and biochemical activities.展开更多
Stomatal regulation is critical for mangroves to survive in the hyper-saline intertidal zone where water stress is severe and water availability is highly fluctuant.However,very little is known about the stomatal sens...Stomatal regulation is critical for mangroves to survive in the hyper-saline intertidal zone where water stress is severe and water availability is highly fluctuant.However,very little is known about the stomatal sensitivity to vapour pressure deficit(VPD)in mangroves,and its co-ordination with stomatal morphology and leaf hydraulic traits.We measured the stomatal response to a step increase in VPD in situ,stomatal anatomy,leaf hydraulic vulnerability and pressure-volume traits in nine true mangrove species of five families and collected the data of genome size.We aimed to answer two questions:(1)Does stomatal morphology influence stomatal dynamics in response to a high VPD in mangroves?with a consideration of possible influence of genome size on stomatal morphology;and(2)do leaf hydraulic traits influence stomatal sensitivity to VPD in mangroves?We found that the stomata of mangrove plants were highly sensitive to a step rise in VPD and the stomatal responses were directly affected by stomatal anatomy and hydraulic traits.Smaller,denser stomata was correlated with faster stomatal closure at high VPD across the species of Rhizophoraceae,and stomata size negatively and vein density positively correlated with genome size.Less negative leaf osmotic pressure at the full turgor(πo)was related to higher operating steady-state stomatal conductance(gs);and a higher leaf capacitance(Cleaf)and more embolism resistant leaf xylem were associated with slower stomatal responses to an increase in VPD.In addition,stomatal responsiveness to VPD was indirectly affected by leaf morphological traits,which were affected by site salinity and consequently leaf water status.Our results demonstrate that mangroves display a unique relationship between genome size,stomatal size and vein packing,and that stomatal responsiveness to VPD is regulated by leaf hydraulic traits and stomatal morphology.Our work provides a quantitative framework to better understand of stomatal regulation in mangroves in an environment with high salinity and dynamic water availability.展开更多
Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in bo...Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in both the natural and vineyard growing conditions. However, information on the recovery responses is relatively scant. Studies were conducted to address this issue using potted vines of the grapevine cultivar, Cabernet Sauvignon, which was subjected to water stress and along with anatomical and ultrastructural characterizations, physiological status was assessed in healthy and water stressed vines, and following recovery via rewatering from the water stressed vines. Water stress induced wilting of leaves, drooping of tendrils, and desiccation followed by abscission of shoot tip leaving behind a brown scar at the shoot apex. The wilted leaves accumulated ABA, which correspondingly reduced stomatal conductance and leaf water potential. Upon re-watering, both these parameters made a recovery with values similar to healthy leaves. Likewise, leaf anatomical features following rewatering resembled to that of healthy leaves. In clusters, water stress caused shriveling of preveraison (unripened) berries, which regained full turgor following water resupply, whereas the postveraison (ripening) berries in the same cluster remained unaffected as evidenced by the presence of viable mesocarp cells and epicuticular wax in the form of platelets. The study revealed that shoot tip with leaf primordia was most sensitive to water stress followed by fully expanded leaves and preveraison berries, whereas the postveraison berries remained unaffected. This information could be valuable to implementing irrigation strategies towards sustaining grape production in existing vineyards experiencing episodic droughts and targeted areas prone to drought.展开更多
Two tolerant (BB24 and BB43) and two susceptible (BARI busbean-2 and BB04) genotypes of common bean (Phaseolus vulgaris L.) were evaluated for their water status and its relationship with reproductive responses under ...Two tolerant (BB24 and BB43) and two susceptible (BARI busbean-2 and BB04) genotypes of common bean (Phaseolus vulgaris L.) were evaluated for their water status and its relationship with reproductive responses under continuous water stress (50% field capacity) and control (80% field capacity) conditions in a net house covered with polyethylene sheet at the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh. Under water stress condition, the susceptible genotype namely BB04 exhibited more negative leaf water potential (LWP) which was followed by that of BARI bushbean-2 in all the time of the day except at noon. The tolerant genotype namely BB24 exhibited less negative LWP at noon. The tolerant genotypes maintained higher relative water content (WRC) than the susceptible ones from dawn to dusk. The relationship between RWC and LWP was examined separately for four genotypes under water stress condition. The genotype BB24 showed a smaller decrease in RWC with more negative LWP than BB04. Water stress reduced pod setting ratio. The relationship between the leaf water status and reproductive responses showed that the genotype with a little reduction in mid-day drop of RWC or with high mid-day RWC displayed a high pod setting ratio.展开更多
Field experiments were conducted in 2008 and 2009 to study the effects of deficit irrigation with saline water on spring wheat growth and yield in an arid region of Northwest China. Nine treatments included three sali...Field experiments were conducted in 2008 and 2009 to study the effects of deficit irrigation with saline water on spring wheat growth and yield in an arid region of Northwest China. Nine treatments included three salinity levels sl, s2 and s3 （0.65, 3.2, and 6.1 dS/m） in combination with three water levels wl, w2 and w3 （375, 300, and 225 mm）. In 2008, for most treatments, deficit irrigation showed adverse effects on wheat growth; meanwhile, the effect of saline irrigation was not apparent. In 2009, growth parameters of wl treatments were not always optimal under saline irrigation. At 3.2 and 6.1 dS/m in 2008, the highest yield was obtained by wl treatments, however, in 2009, the weight of 1,000 grains and wheat yield both followed the order w2 〉 wl 〉 w3. In this study, spring wheat was sensitive to water deficit, especially at the booting to grain-filling stages, but was not significantly affected by saline irrigation and the combination of the two factors. The results demonstrated that 300-mm irrigation water with a salinity of less than 3.2 dS/m is suitable for wheat fields in the study area.展开更多
Hydraulic resistance components and water relations were studied on Haloxyolon ammoden-dron,a small xeric tree,growing at sites significantly differed in soil texture.Soil water content,leaf water potential(ψl),xylem...Hydraulic resistance components and water relations were studied on Haloxyolon ammoden-dron,a small xeric tree,growing at sites significantly differed in soil texture.Soil water content,leaf water potential(ψl),xylem water potential(ψx),root water potential(ψroot),leaf transpiration rate(TR) and stomatal conductance(gs) were measured at the two sites during the growing season of 2005 and 2006.Leaf spe-cific hydraulic resistance(Rplant) during the whole growing season,hydraulic resistance of plants(Rp),shoots(Rshoot) and roots(Rroot) in the August of both years were calculated and expressed on leaf area basis.The results showed the proportion of the hydraulic resistance of the aerial part(Rshoot) to the Rp was the same to the proportion of the hydraulic resistance of the soil part(Rroot) to the Rp,indicating that both parts were equivalent important to plant water hydraulic system from soil to leaf.Positive significant corre-lations were found between Rp and Rroot,suggesting that root hydraulics resistance was a major determinant of plant hydraulic resistance(Rp) and transpiration rate.The integrated effect of stomatal control,hy-draulic regulation and morphology adjustment enabled plants at heavy soil site surviving the extreme water deficit period.展开更多
Canopy temperature (CT), leaf water potential (LWP) and spikelet fertility (SF) of a set of RILs (F9) from the cross between Zhenshan97B and IRAT109 were investi- gated under two soil moisture regimes in a drought scr...Canopy temperature (CT), leaf water potential (LWP) and spikelet fertility (SF) of a set of RILs (F9) from the cross between Zhenshan97B and IRAT109 were investi- gated under two soil moisture regimes in a drought screen facility. In water stress condition, CT was negatively corre- lated with SF (r = ?0.2867) and LWP (r = ?0.2740), and LWP was positively associated with SF (r = 0.1696). These results indicated that the plant with high drought tolerant ability could maintain higher LWP and lower CT, leading to higher SF. A total of 44 main effect QTLs were associated with CT, LWP and SF. The accumulated contributionsof QTLs forCT, LWP and SF were 87.85%, 15.06%, 79.46% under well water condition and 72.61%, 87.68%, 33.29% under stress condi- tion, respectively. Totally 45 pairs of digenic interactions were detected. The accumulated contribution of digenic epistasis on CT, LWP and SF were 55.69%, 47.15%, 48.15% under well water condition and 53.44%, 57.94%, 54.62% under water stress condition. Compared with other drought tolerance QTL mapping researches in rice, 19 main effect QTLs were found to be located at the same or closely con- joint regions.展开更多
Forests over limestone in the tropics have received little attention and limestone forests in Vietnam have been overlooked to an even greater extent in terms of tree physiology. In Ba Be National Park, Vietnam, soil w...Forests over limestone in the tropics have received little attention and limestone forests in Vietnam have been overlooked to an even greater extent in terms of tree physiology. In Ba Be National Park, Vietnam, soil water availability in limestone forests seems to be the most limiting factor in the dry season. Therefore, in order to enhance the preliminary knowledge of choosing native tree species for enrichment planting in the restoration zone, characteristics of the 20 native tree species to soil water stress were investigated in a limestone forest. One-ha plot each consisting of twenty-five 20 m × 20 m plots was established in undisturbed forests. All trees ≥ 10 cm DBH were measured in 20 m × 20 m plots, while twenty-five 5 m × 5 m subplots were established in order to sample the regeneration of tree species with a DBH < 10 cm. The Scholander apparatus and freezing point osmometry were used in order to measure the leaf water potential (Ψw) and leaf osmotic potential (Ψπ) of the 20 native tree species, respectively in this study. 61 species belonging to 34 families of all trees with a DBH ≥ 10 cm were recorded in one ha, while 31 species representing 18 families of trees < 10 cm DBH were identified in 625 m2. The 20 species’ leaf water and osmotic potential values revealed significant differences among species. The maximum leaf water potential was not affected by any anticipated sources of variation, while the minimum water potential, however, showed significant variation to soil water stress. The results in the study area emphasized the importance of water factors in influencing tree species distribution;it could be concluded that native species with wide water potential ranges would be better able to withstand water changes and might be thus good candidates for reforestation (enrichment planting) in limestone areas.展开更多
文摘The study on the changes of stomatal sensitivity in relation to xylem ABA during periodical soil drying and the effect of leaf water status on the stomatal sensitivity has confirmed that xylem ABA concentration is a good indicator of soil water status around roots and the relation between xylem ABA concentration and predawn leaf water potential remained constant during the three consecutive soil drying cycles based on the slopes of the fitted lines. The sensitivity of stomata to xylem ABA increased substantially as the soil drying cycles progressed, and the xylem ABA concentration needed to cause a 50% decrease of stomatal conductance was as low as 550 nmol/L in the next two soil drying cycle, as compared with the 750 nmol/L ABA in the first cycle of soil drying. The results using the split_root system showed that leaf water deficit significantly enhanced the stomatal response to xylem ABA and the xylem ABA concentration needed to cause a 50% decrease in stomatal conductance was 2 to 4 times smaller in the whole_root_drying treatment than those in the semi_root_drying treatment. These results suggested that the sensitivity of stomata to xylem ABA concentration is not a fixed characteristic.
文摘The water deficit in arid and semi-arid regions is the primary limiting factor for the development of urban greenery and forestation. In addition, planting the species that consume low levels of water is useful in arid and semi-arid regions that have poor water management measures. Leaf water potential(Ψ) is a physiological parameter that can be used to identify drought resistance in various species. Indeed, Ψ is one of the most important properties of a plant that can be measured using a pressure chamber. Drought avoiding or drought resistant species have a lower Ψ than plants that use normal or high levels of water. To determine drought resistance of species that are suitable for afforestation in arid urban regions, we evaluated twenty woody species in the Isfahan City, central Iran. The experimental design was random split-split plots with five replications. The species were planted outdoor in plastic pots and then subjected to treatments that consisted of two soil types and five drip irrigation regimes. To evaluate the resistance of each species to drought, we used the Ψ and the number of survived plants to obtain the drought resistance index(DRI). Then, cluster analysis, dendrogram, and similarity index were used to group the species using DRI. Result indicates that the evaluated species were classified into five groups:(1) high water consuming species(DRI>–60 MPa);(2) above normal water consuming species(–60 MPa≥DRI>–90 MPa);(3) normal water consuming species(–90 MPa≥DRI>–120 MPa);(4) semi-drought resistant species(–120 MPa≥DRI>–150 MPa);and(5) drought resistant species(DRI≤–150 MPa). According to the DRI, Salix babylonica L., Populus alba L., and P. nigra L. are high water consuming species, Platanus orientalis L. and Albizia julibrissin Benth are normal water consuming species, and Quercus infectoria Oliv. and Olea europaea L. can be considered as drought resistant species.
基金The study was funded by the National Natural Science Foundation of China(41571130082,41371507).
文摘Regulation of leaf gas exchange plays an important role in the survival of trees and shrubs under future climate change. However, the responses of leaf water potential and gas exchange of shrubs in semi-arid areas to the precipitation alteration are not clear. Here, we conducted a manipulated experiment with three levels of precipitation, i.e., a control with ambient precipitation, 50% above ambient precipitation(irrigation treatment), and 50% below ambient precipitation(drought treatment), with two common shrubs, Salix psammophila C. Wang & C. Y. Yang(isohydric plant, maintaining a constant leaf water potential by stomatal regulation) and Caragana korshinskii Kom.(anisohydric plant, having more variable leaf water potential), on the Chinese Loess Plateau in 2014 and 2015. We measured the seasonal variations of predawn and midday leaf water potential(Ψpd and Ψmd), two parameters of gas exchange, i.e., light-saturated assimilation(An) and stomatal conductance(gs), and other foliar and canopy traits. The isohydric S. psammophila had a similar An and a higher gs than the anisohydric C. korshinskii under drought treatment in 2015, inconsistent with the view that photosynthetic capacity of anisohydric plants is higher than isohydric plants under severe drought. The two shrubs differently responded to precipitation manipulation. Ψpd, An and gs were higher under irrigation treatment than control for S. psammophila, and these three variables and Ψmd were significantly higher under irrigation treatment and lower under drought treatment than control for C. korshinskii. Leaf water potential and gas exchange responded to manipulated precipitation more strongly for C. korshinskii than for S. psammophila. However, precipitation manipulation did not alter the sensitivity of leaf gas exchange to vapor-pressure deficit and soil moisture in these two shrubs. Acclimation to long-term changes in soil moisture in these two shrubs was primarily attributed to the changes in leaf or canopy structure rather than leaf gas exchange. These findings will be useful for modeling canopy water-carbon exchange and elucidating the adaptive strategies of these two shrubs to future changes in precipitation.
文摘Seedlings of drought-tolerance rice varieties Han 501and Han A03,and the drought sensitive varietiesNanjing 11 and Yanjing 2 were raised in a paddyfield and transplanted into pots at the age of 8leaves.Water stress started at the tillering stage byholding water from 0 MPa of the soil water potentialin pots till the leaves showed seriously wilting.
文摘Differences in transpiration and leaf water potential （LWP） in relation to cassava yield were investigated along inland valley toposequence in a 4×4 Latin square design. The landrace with the highest transpiration rate and lowest LWP yielded the lowest, while TMS 91/02324 and TMS 91/02327 with intermediate rate and highest LWP yielded the highest, indicating that high transpiration rate associated with low LWP reduced yield. Transpiration was lower in the fringe with deeper water table than valley bottom at deep water table site, while at shallow water table, it was higher in the fringe than valley bottom, suggesting that drought and excess moisture reduced transpiration. LWP and water table depth correlated negatively indicating that shallow water table reduced transpiration by reducing LWP. Transpiration increased and LWP decreased as radiation, leaf temperature and vapour pressure deficit increased and differences in these microclimatic conditions caused differences in the two processes between sites, years and time of day. Under mild water stress, transpiration and LWP were higher in the afternoon than the morning, but the reverse occurred under severe stress. TMS 91/02324 and TMS 91/02327 had the highest LWP under severe stress, indicating their higher drought tolerance than the other cultivars.
文摘A greenhouse pot experiment was conducted using a complete random design with six replications. A pressure-volume curve analysis was employed to study the effects of organic fertilizers on plant water relations in sweet corn (Zea mays L. cv. Honey-bantam) in terms of leaf osmotic concentration (Cs), osmotic potentials at full turgid status (πFT) and at incipient plasmolysis (πIP), and of symplastic (ζsym) and apoplastic (ζapo) fractions in the tissue water compartment in relation to photosynthetic capacity (Pc) and dry mass accumulation. At the seedling stage (day 15), plants with chemical fertilizer treatments showed lower πFT, πIP and ζapo and higher Cs, ζsym and PC than those with organic fertilizer treatments. Compared to PC and relative growth, where values from day 15 to day 75 were first lower for organic treatments and then higher, ζsym and Cs values were similar, while πFT and πIP were opposite being higher then lower. Dry mass production with organic fertilizer was higher than or close to the chemical fertilizer treatments in the later growth stage (day 75), though dry mass production with chemical fertilizers was much higher in the early and middle growing stages (days 15 and 45). Increased photosynthesis and dry mass production in later growth stages due to organic fertilizers were associated with increased osmotic concentration in the tissue and the symplastic fraction of the tissue water compartment. These might favor stomatal opening and biochemical activities.
基金financially supported by a grant of the National Natural Science Foundation of China(31670406)the Bagui Fellow scholarship(C33600992001)of Guangxi Zhuang Autonomous Region to KFC.
文摘Stomatal regulation is critical for mangroves to survive in the hyper-saline intertidal zone where water stress is severe and water availability is highly fluctuant.However,very little is known about the stomatal sensitivity to vapour pressure deficit(VPD)in mangroves,and its co-ordination with stomatal morphology and leaf hydraulic traits.We measured the stomatal response to a step increase in VPD in situ,stomatal anatomy,leaf hydraulic vulnerability and pressure-volume traits in nine true mangrove species of five families and collected the data of genome size.We aimed to answer two questions:(1)Does stomatal morphology influence stomatal dynamics in response to a high VPD in mangroves?with a consideration of possible influence of genome size on stomatal morphology;and(2)do leaf hydraulic traits influence stomatal sensitivity to VPD in mangroves?We found that the stomata of mangrove plants were highly sensitive to a step rise in VPD and the stomatal responses were directly affected by stomatal anatomy and hydraulic traits.Smaller,denser stomata was correlated with faster stomatal closure at high VPD across the species of Rhizophoraceae,and stomata size negatively and vein density positively correlated with genome size.Less negative leaf osmotic pressure at the full turgor(πo)was related to higher operating steady-state stomatal conductance(gs);and a higher leaf capacitance(Cleaf)and more embolism resistant leaf xylem were associated with slower stomatal responses to an increase in VPD.In addition,stomatal responsiveness to VPD was indirectly affected by leaf morphological traits,which were affected by site salinity and consequently leaf water status.Our results demonstrate that mangroves display a unique relationship between genome size,stomatal size and vein packing,and that stomatal responsiveness to VPD is regulated by leaf hydraulic traits and stomatal morphology.Our work provides a quantitative framework to better understand of stomatal regulation in mangroves in an environment with high salinity and dynamic water availability.
文摘Among all fruit crops of horticultural importance, grapevines (Vitis vinifera L.) stand out as the most drought tolerant crop species whose tolerance is credited to their proficiency to recover from water stress in both the natural and vineyard growing conditions. However, information on the recovery responses is relatively scant. Studies were conducted to address this issue using potted vines of the grapevine cultivar, Cabernet Sauvignon, which was subjected to water stress and along with anatomical and ultrastructural characterizations, physiological status was assessed in healthy and water stressed vines, and following recovery via rewatering from the water stressed vines. Water stress induced wilting of leaves, drooping of tendrils, and desiccation followed by abscission of shoot tip leaving behind a brown scar at the shoot apex. The wilted leaves accumulated ABA, which correspondingly reduced stomatal conductance and leaf water potential. Upon re-watering, both these parameters made a recovery with values similar to healthy leaves. Likewise, leaf anatomical features following rewatering resembled to that of healthy leaves. In clusters, water stress caused shriveling of preveraison (unripened) berries, which regained full turgor following water resupply, whereas the postveraison (ripening) berries in the same cluster remained unaffected as evidenced by the presence of viable mesocarp cells and epicuticular wax in the form of platelets. The study revealed that shoot tip with leaf primordia was most sensitive to water stress followed by fully expanded leaves and preveraison berries, whereas the postveraison berries remained unaffected. This information could be valuable to implementing irrigation strategies towards sustaining grape production in existing vineyards experiencing episodic droughts and targeted areas prone to drought.
文摘Two tolerant (BB24 and BB43) and two susceptible (BARI busbean-2 and BB04) genotypes of common bean (Phaseolus vulgaris L.) were evaluated for their water status and its relationship with reproductive responses under continuous water stress (50% field capacity) and control (80% field capacity) conditions in a net house covered with polyethylene sheet at the Bangabandhu Sheikh Mujibur Rahman Agricultural University, Bangladesh. Under water stress condition, the susceptible genotype namely BB04 exhibited more negative leaf water potential (LWP) which was followed by that of BARI bushbean-2 in all the time of the day except at noon. The tolerant genotype namely BB24 exhibited less negative LWP at noon. The tolerant genotypes maintained higher relative water content (WRC) than the susceptible ones from dawn to dusk. The relationship between RWC and LWP was examined separately for four genotypes under water stress condition. The genotype BB24 showed a smaller decrease in RWC with more negative LWP than BB04. Water stress reduced pod setting ratio. The relationship between the leaf water status and reproductive responses showed that the genotype with a little reduction in mid-day drop of RWC or with high mid-day RWC displayed a high pod setting ratio.
基金supported by the National Basic Research Program of China (2011CB403406)the National Natural Science Foundation of China (51179166)the Youth Foundation of Taiyuan University of Technology (2012L077)
文摘Field experiments were conducted in 2008 and 2009 to study the effects of deficit irrigation with saline water on spring wheat growth and yield in an arid region of Northwest China. Nine treatments included three salinity levels sl, s2 and s3 （0.65, 3.2, and 6.1 dS/m） in combination with three water levels wl, w2 and w3 （375, 300, and 225 mm）. In 2008, for most treatments, deficit irrigation showed adverse effects on wheat growth; meanwhile, the effect of saline irrigation was not apparent. In 2009, growth parameters of wl treatments were not always optimal under saline irrigation. At 3.2 and 6.1 dS/m in 2008, the highest yield was obtained by wl treatments, however, in 2009, the weight of 1,000 grains and wheat yield both followed the order w2 〉 wl 〉 w3. In this study, spring wheat was sensitive to water deficit, especially at the booting to grain-filling stages, but was not significantly affected by saline irrigation and the combination of the two factors. The results demonstrated that 300-mm irrigation water with a salinity of less than 3.2 dS/m is suitable for wheat fields in the study area.
基金supported by the National Natural Science Foundation of China (40971042)the Western Light project of the Chinese Academy of Sciences (XBBS 200808)
文摘Hydraulic resistance components and water relations were studied on Haloxyolon ammoden-dron,a small xeric tree,growing at sites significantly differed in soil texture.Soil water content,leaf water potential(ψl),xylem water potential(ψx),root water potential(ψroot),leaf transpiration rate(TR) and stomatal conductance(gs) were measured at the two sites during the growing season of 2005 and 2006.Leaf spe-cific hydraulic resistance(Rplant) during the whole growing season,hydraulic resistance of plants(Rp),shoots(Rshoot) and roots(Rroot) in the August of both years were calculated and expressed on leaf area basis.The results showed the proportion of the hydraulic resistance of the aerial part(Rshoot) to the Rp was the same to the proportion of the hydraulic resistance of the soil part(Rroot) to the Rp,indicating that both parts were equivalent important to plant water hydraulic system from soil to leaf.Positive significant corre-lations were found between Rp and Rroot,suggesting that root hydraulics resistance was a major determinant of plant hydraulic resistance(Rp) and transpiration rate.The integrated effect of stomatal control,hy-draulic regulation and morphology adjustment enabled plants at heavy soil site surviving the extreme water deficit period.
基金This study was supported by the Hi-Technology Research and Development Program of China(Grant No.2003AA207010)the National Basic Research Program of China(Grant No.2004CB 117204)..
文摘Canopy temperature (CT), leaf water potential (LWP) and spikelet fertility (SF) of a set of RILs (F9) from the cross between Zhenshan97B and IRAT109 were investi- gated under two soil moisture regimes in a drought screen facility. In water stress condition, CT was negatively corre- lated with SF (r = ?0.2867) and LWP (r = ?0.2740), and LWP was positively associated with SF (r = 0.1696). These results indicated that the plant with high drought tolerant ability could maintain higher LWP and lower CT, leading to higher SF. A total of 44 main effect QTLs were associated with CT, LWP and SF. The accumulated contributionsof QTLs forCT, LWP and SF were 87.85%, 15.06%, 79.46% under well water condition and 72.61%, 87.68%, 33.29% under stress condi- tion, respectively. Totally 45 pairs of digenic interactions were detected. The accumulated contribution of digenic epistasis on CT, LWP and SF were 55.69%, 47.15%, 48.15% under well water condition and 53.44%, 57.94%, 54.62% under water stress condition. Compared with other drought tolerance QTL mapping researches in rice, 19 main effect QTLs were found to be located at the same or closely con- joint regions.
基金support by the Open Access Publication Funds of the Gottingen University
文摘Forests over limestone in the tropics have received little attention and limestone forests in Vietnam have been overlooked to an even greater extent in terms of tree physiology. In Ba Be National Park, Vietnam, soil water availability in limestone forests seems to be the most limiting factor in the dry season. Therefore, in order to enhance the preliminary knowledge of choosing native tree species for enrichment planting in the restoration zone, characteristics of the 20 native tree species to soil water stress were investigated in a limestone forest. One-ha plot each consisting of twenty-five 20 m × 20 m plots was established in undisturbed forests. All trees ≥ 10 cm DBH were measured in 20 m × 20 m plots, while twenty-five 5 m × 5 m subplots were established in order to sample the regeneration of tree species with a DBH < 10 cm. The Scholander apparatus and freezing point osmometry were used in order to measure the leaf water potential (Ψw) and leaf osmotic potential (Ψπ) of the 20 native tree species, respectively in this study. 61 species belonging to 34 families of all trees with a DBH ≥ 10 cm were recorded in one ha, while 31 species representing 18 families of trees < 10 cm DBH were identified in 625 m2. The 20 species’ leaf water and osmotic potential values revealed significant differences among species. The maximum leaf water potential was not affected by any anticipated sources of variation, while the minimum water potential, however, showed significant variation to soil water stress. The results in the study area emphasized the importance of water factors in influencing tree species distribution;it could be concluded that native species with wide water potential ranges would be better able to withstand water changes and might be thus good candidates for reforestation (enrichment planting) in limestone areas.
