[Objectives]This study was conducted to increase the reproduction coefficient of Japanese honeysuckle(Lonicera japonica)to keep the character of optimal benign.[Methods]The young leaves of medicinal Japanese honeysuck...[Objectives]This study was conducted to increase the reproduction coefficient of Japanese honeysuckle(Lonicera japonica)to keep the character of optimal benign.[Methods]The young leaves of medicinal Japanese honeysuckle were selected as explants,and MS was used as the basic culture medium.Suitable culture concentrations and conditions were screened through different concentration gradients of growth regulators and cytokinin.[Results]As the concentration of 6-BA in the culture medium increased,the browning rate increased,and the browning phenomenon occurred earlier.On the contrary,a lower concentration of 6-BA was suitable for the differentiation and growth of young leaves,and the browning response was slow.However,if the cultivation time was too long and the materials were not transferred in a timely manner,browning would also occur.The optimal combination of levels was obtained through a 3×3 orthogonal experiment(three parallel groups for each of 6-BA and NAA).The culture conditions included a constant temperature of 26℃and light intensity of 1200 lx.The optimal medium for inducing callus proliferation was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;and the optimal medium for inducing bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.[Conclusions]This study provides a theoretical basis for accelerating the development of the honeysuckle industry.展开更多
[Objectives]To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination.[Methods]The total flavonoids of L.japonica were extracted b...[Objectives]To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination.[Methods]The total flavonoids of L.japonica were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of L.japonica.[Results]The optimal extraction process of the total flavonoids of L.japonica was 70%ethanol,1∶30 of solid-to-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in L.japonica and is expected to provide a certain scientific basis for the study of the quality standard of L.japonica.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from L.japonica.展开更多
Background:To study the protective effect of different medicinal parts of honeysuckle on myocardial ischemia injury in rats.Method:SD mice were randomly divided into 6 groups:the normal group,model group,compound salv...Background:To study the protective effect of different medicinal parts of honeysuckle on myocardial ischemia injury in rats.Method:SD mice were randomly divided into 6 groups:the normal group,model group,compound salvia miltiorrhiza group,Lonicera japonica Thunb.of flower bud group,flower group,and the stem leaf group.There were 8 rats in each group,including 4 males and 4 females.The normal group and model group were given equal amount of distilled water,whereas the other dosage groups were given the appropriate drug by intragastric administration,continuous gavage for 14 days.From the 12 days,in addition to the normal group were injected of the same amount of normal saline,the other groups were injected with 5g/kg.Injection of isoproterenol to establish the mouce mycardial ischemia model,its continuously 3d.To detect the serum of AST,CK,CK-MB,LDH and the myocardial issue of Chinese super oxideismutase and malondialdehyde level.Results:Compared with normal group,the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde were significantly increased in the model group(P<0.01)and the level of super oxideismutase decreased significantly(P<0.01)the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde in the serum of flower bud group,flower group and stem leaf group were significantly decreased(P<0.05),and the decreasing level of flower bud group,flower,leaf group of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde was flower bud group>flower>leaf group,the activity of super oxideismutase was obviously increased,and the activity level of super oxideismutase of flower bud group,flower group and stem leaf group was flower bud group>flower>leaf group.Conclusion:In the myocardial ischemic injury model of rats induced by isoproterenol,Lonicera japonica Thunb.had a protective effect in all parts,and Lonicera japonica Thunb.bud had the greatest protective effect.展开更多
A new triterpenoid saponin, together with a known saponin, was isolated from the flowers of Lonicera Japonica Thunb. Using chemical and spectroscopic methods. mainly 2D NMR technique. their structures were deduced to ...A new triterpenoid saponin, together with a known saponin, was isolated from the flowers of Lonicera Japonica Thunb. Using chemical and spectroscopic methods. mainly 2D NMR technique. their structures were deduced to be 3-β-D-glucopyranosyl-(1-4)-β-D-glucopyranosyl- (1 - 3)-a-L-rhamnospyranosyl-(1 - 2)-a-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl- (1 - 6)-β-D-glucopyranoside and 3-a-L-rhamnospyranosyl-(1 - 2)-a- Larabinopyranosyl hederagenin 28-O-a-L-rhamnospyranosyl - (1 - 2 )- [β - D- xylcopyranosyl-(1 - 6)]-β-D-glucopyranoside. respectively.展开更多
Objective:To examine the acanthamoebicidal effects of ethyl acetate,aqueous and butanol fractions of dried flower buds of Lonicera japonica(L.japonica) Thunb.(Flos Lonicerae) in vitro.Methods:Acanthamoeba triangularis...Objective:To examine the acanthamoebicidal effects of ethyl acetate,aqueous and butanol fractions of dried flower buds of Lonicera japonica(L.japonica) Thunb.(Flos Lonicerae) in vitro.Methods:Acanthamoeba triangularis isolates were obtained from environmental water samples and identified by PCR.They were exposed to ethyl acetate,water and butanol fractions of L.japonica Thunb.at concentrations ranging from 0.5 mg/m L to 1.5 mg/m L.The extracts were evaluated for growth inhibition at 24,48 and 72 h,respectively.Chlorogenic acid at a concentration of 1 mg/mL was examined for inhibition of encystment.Results:Ethyl acetate fraction at a concentration of 1.5 mg/mL evoked a significant reduction of trophozoite viability by 48.9% after 24 h,49.2% after 48 h and 33.7% after 72 h Chlorogenic acid,the major active constituent of L.japonica Thunb.at the concentration of 1 mg/mL reduced the cysts/trophozoite ratio by 100% after 24 h,84.0% after 48 h and 72.3% after 72 h.This phenolic compound at concentration of 1 mg/mL concurrent with 0.6% hydrogen peroxide inhibited hydrogen peroxide-induced encystment by 92.8% at 72 h.Conclusions:Results obtained from this study show that ethyl acetate fraction at 1.5 mg/m L is the most potent fraction of L.japonica Thunb.and its major constituent chlorogenic acid showed the remarkable inhibition of encystment at a concentration of 1 mg/mL.展开更多
Lonicera japonica Thunb. is a typical medicinal and edible plant in China. It has antibacterial, anti-oxidant, anti-endotoxin, and anti-inflammatory effects, etc. Its pharmacological action is closely related to its a...Lonicera japonica Thunb. is a typical medicinal and edible plant in China. It has antibacterial, anti-oxidant, anti-endotoxin, and anti-inflammatory effects, etc. Its pharmacological action is closely related to its active components. Based on the results of previous studies, this paper summarized the effects of variety, origin, cultivation conditions, pruning, harvesting period and processing methods on the active components of L. japonica , in the hope of providing theoretical guidance for high-yield and high-quality cultivation of L. japonica.展开更多
BACKGROUND: It has been shown that interleukin-1β (IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2,which binds with receptors of ...BACKGROUND: It has been shown that interleukin-1β (IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2,which binds with receptors of the thermo-sensitive hypothalamic neurons. Lonicera japonica is one of the medicinal plants used widely in Asia for its antipyretic properties. However,these mechanisms have not yet been intensively studied. OBJECTIVE: To investigate the antipyretic effect and mechanisms of Lonicera japonica on IL-1β-induced febrile New Zealand rabbits by observing expression changes of E-type prostaglandin receptor-3 (EP3) mRNA in the preoptic anterior hypothalamus (POAH). DESIGN: A randomized controlled study. SETTING: Electrophysiological Laboratory at the Department of Pathophysiology,Medical College of Jinan University; Department of Orthopaedics,First Hospital Affiliated to Medical College of Jinan University. MATERIALS: The experiment was performed from April to December 2005,using a total of 32 New Zealand white rabbits of both sexes,weighing 1.5-2.0 kg. All the animal experiments were performed according to the internationally accepted ethical guidelines. Lonicera japonica injection was purchased from Huanghe pharmaceutical factory of Xi'an,China. IL-1β was purchased from Sigma,USA. METHODS: A total of 32 rabbits were divided randomly into four groups: ① Normal saline (NS) control group; ② Lonicera japonica treatment group; ③ IL-1β treatment group; and ④ Lonicera japonica plus IL-1β treatment group. In the first 3 groups,the rabbits were given separate intravenous (i.v.) injections of 1 mL NS,1 mL Lonicera japonica,and 100 ng IL-1β (dissolved in 0.9% NS without pyrogen). In the Lonicera japonica plus IL-1β group each rabbit was given i.v. injections of 1 mL NS and,30 minutes later,100 ng IL-1β. MAIN OUTCOME MEASURES: Colonic temperature of each rabbit was measured at 0,10,20,30,40,50,60,and 70 minutes after injection and the maximum temperature rise (ΔT) and the temperature response index after 1 hour (TRI1) was calculated. Subsequently,in situ hybridization (ISH) was done with an ISH kit,EP3 mRNA expression in the POAH of all groups was measured (number of positive cells and average gray scale value). RESULTS: A total of 32 rabbits were included in the result analysis,without any loss. ①ΔT and TRI1: there was no significant difference between the Lonicera japonica group and NS group (P > 0.05). The IL-1β group was significantly greater compared to NS group (P < 0.01). The Lonicera japonica plus IL-1β group was significantly less than the IL-1β group (P < 0.05). ② In the NS and Lonicera japonica groups,the EP3 mRNA expression was negative (no coloration) or only weakly positive (only a few brown yellow particles in the cytoplasm cells could not be identified). The number of positive cells and the average gray scale value were not significantly different between the two groups (P > 0.05). In the IL-1β group,the number of positive cells was remarkably higher and the average gray scale value was lower than the NS group (P < 0.01). In the Lonicera japonica plus IL-1β group,the number of positive cells was significantly less than the IL-1β group (P < 0.05). However,the average gray scale value was greater than the IL-1β group (P < 0.05). CONCLUSION: Lonicera japonica has obvious antipyretic effects on IL-1β-induced febrile rabbits and acts by inhibiting expression of EP3 mRNA in the POAH.展开更多
Lonicera japonica,also known as honeysuckle,is an evergreen shrub in the family of Syzygium.By consulting Sciencedirect databases and Web of Science databases,79 related articles were found,of which 22 were related to...Lonicera japonica,also known as honeysuckle,is an evergreen shrub in the family of Syzygium.By consulting Sciencedirect databases and Web of Science databases,79 related articles were found,of which 22 were related to chemical composition and pharmacological activity.These articles show that L.japonica has a wide range of pharmacological activities,including antiviral,anti-tumor,and antioxidant effects.These activities have important applications in the pharmaceutical,food,and fragrance industries.This review focuses on the chemical composition and pharmacological effects of L.japonicae,which is of great significance to the development of new drugs and therapeutic methods.展开更多
Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects o...Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF.Fingerprints of ten batches of LF were established by UPLC-PDA.The inhibitory levels of AST and ALT were used as pharmacological indexes,and secoxyloganin,isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis(GRA)and partial least squares regression analysis(PLSR).Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds.Molecular docking is used to explore the interaction between small molecules and proteins.This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF,which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.展开更多
Three new glucosylated caffeoylquinic acid isomers(1–3),along with six known compounds,have been isolated from an aqueous extract of the flower buds of Lonicera japonica.Structures of the new compounds were determine...Three new glucosylated caffeoylquinic acid isomers(1–3),along with six known compounds,have been isolated from an aqueous extract of the flower buds of Lonicera japonica.Structures of the new compounds were determined by spectroscopic and chemical methods as()-4-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(1),()-3-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(2),and()-5-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(3),respectively.In the preliminary in vitro assays,two known compounds methyl caffeate and 2?-O-methyladenosine showed inhibitory activity against Coxsackie virus B3 with IC50 values of 3.70 μmol/L and 6.41 μmol/L and SI values of 7.8 and 12.1,respectively.展开更多
The anti-bacterial activities of three types of di-O-caffeoylquinic acids(diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine(TCM), on Bacillus shigae growth were investigated and compared by microcal...The anti-bacterial activities of three types of di-O-caffeoylquinic acids(diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine(TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid(3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid(3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid(4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power–time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs(IC50) were obtained by quantitative analysis. Based on the quantity–activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.展开更多
Lonicera japonica Thunb.,a traditional Chinese herb,has been used for treating human diseases for thousands of years.Recently,the genome of L.japonica has been decoded,providing valuable information for research into ...Lonicera japonica Thunb.,a traditional Chinese herb,has been used for treating human diseases for thousands of years.Recently,the genome of L.japonica has been decoded,providing valuable information for research into gene function.However,no comprehensive database for gene functional analysis and mining is available for L.japonica.We therefore constructed LjaFGD(www.gzybioinformatics.cn/LjaFGD and bioinformatics.cau.edu.cn/LjaFGD),a database for analyzing and comparing gene function in L.japonica.We constructed a gene co-expression network based on 77 RNA-seq samples,and then annotated genes of L.japonica by alignment against protein sequences from public databases.We also introduced several tools for gene functional analysis,including Blast,motif analysis,gene set enrichment analysis,heatmap analysis,and JBrowse.Our co-expression network revealed that MYB and WRKY transcription factor family genes were co-expressed with genes encoding key enzymes in the biosynthesis of chlorogenic acid and luteolin in L.japonica.We used flavonol synthase 1(LjFLS1)as an example to show the reliability and applicability of our database.LjaFGD and its various associated tools will provide researchers with an accessible platform for retrieving functional information on L.japonica genes to further biological discovery.展开更多
Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the str...Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the structures were identified by spectroscopic analyses.Lonijapoglycol A(1)expressed significant anti-inflammatory activity to inhibit the release ofβ-glucuronidase induced by platelet-activating factor in rat polymorphonuclear leukocytes with an IC50 value of 3.76μmol·L^-1.展开更多
Two new homosecoiridoids,named loniaceticiridoside(l)and lonimalondialiridoside(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures including the absolute configuration we...Two new homosecoiridoids,named loniaceticiridoside(l)and lonimalondialiridoside(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures including the absolute configuration were determined by extensive spectroscopic studies,especially by 2D NMR and CD data analysis.A proposed biosynthetic pathway and preliminary investigations of the biological activity of compounds 1 and 2 are also discussed.展开更多
Two new l-(6'-O-acyl-β-D-glucopyranosyl)pyridinium-3-carboxylates,lonijaponinicotinosides A(1)and B(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures were determine...Two new l-(6'-O-acyl-β-D-glucopyranosyl)pyridinium-3-carboxylates,lonijaponinicotinosides A(1)and B(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures were determined by spectroscopic data analysis,and confirmed by comparison with synthetic 1-β-Dglucopyranosylpyridinium-3-carboxylate.展开更多
OBJECTIVE: To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb.(BLJ and FLJ) using in vitro assays.METHODS: We assessed the in vitro antioxidant activities of aqueous ...OBJECTIVE: To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb.(BLJ and FLJ) using in vitro assays.METHODS: We assessed the in vitro antioxidant activities of aqueous extracts of BLJ and FLJ and compared with that of classical antioxidants vitamin C and butylated hydroxytoluene, using several well-established methods including the 1,1-diphenyl-2-picryl-hydrazyl assay, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay, reducing power assay, fluorescence recovery after photobleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: The aqueous extracts of both BLJ and FLJ had similarly potent antioxidant capacity. There were no significance differences between BLJ and FLJ in all the assays.CONCLUSION: The aqueous extracts of both BLJ and FLJ have antioxidant activity with comparable efficacy. These findings suggest that both BLJ and FLJ may have the potential as natural antioxidants.展开更多
Lonicerae japonicaeFlos (LJF) is widely used in traditional Chinese medicines for the treatment of various diseases, which is now in great demand every year and has a broad development prospect. However, the flowering...Lonicerae japonicaeFlos (LJF) is widely used in traditional Chinese medicines for the treatment of various diseases, which is now in great demand every year and has a broad development prospect. However, the flowering phase of common LJF varieties is so short, which seriously restricts the development of LJF industry. As a new cultivated kind of Lonicerae japonicae Flos, “Hua Jin 6” has characteristics in long flowering phase and conveniently picking, which makes it have a broad development prospect. The aim of this study is to provide scientific guidance for its suitable harvest period by measuring yield and quality of “Hua Jin 6” from different harvest time. Studies show that flower size had a slowly rising trend from the first day to the seventh day, and then slowly declined or kept stable. There were no significant differences of total phenolic acid contents in different samples from different days, but contents of total flavonoids were on the rise and up to maximum in the ninth day. The contents of total iridoids had an increasing tendency from the first day to the fifth day and then kept relatively stable in other days. We demonstrated that the quality of “Hua Jin 6” is relatively stable and suitable for harvesting in all flower buds white stage in term of HPLC fingerprints. Our findings can make it possible to select the suitable time for different harvest purpose.展开更多
OBJECTIVE Rheumatoid arthritis(RA)is the most common inflammatory autoimmune disease,affecting around 1% of the world population.Toll-like receptor 4(TLR4)signalling has been found to be involved in the pathogenesis o...OBJECTIVE Rheumatoid arthritis(RA)is the most common inflammatory autoimmune disease,affecting around 1% of the world population.Toll-like receptor 4(TLR4)signalling has been found to be involved in the pathogenesis of RA.It is a potential therapeutic target for RA treatment.A herbal formula(RL)consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos has traditionally been used in treating various inflammatory disorders.In this study,we would evaluate the anti-arthritic effect of RL on collagen-induced arthritis(CIA)in rats and investigate the involvement of TLR4 signaling in the mode of action of RL in vivo and in vitro.METHODS In vivo anti-arthritic efficacy was evaluated using CIA rats induced by bovine typeⅡ collagen.The treatment groups were treated with various concentrations of RL or positive control indomethacin for 35 d.Clinical signs(hind paw volume and arthritis severity scores),changes in serum inflammatory mediators,histological and radiographic changes of joints were investigated.Spleens and peritoneal macrophages were used to determine the effects of RL on innate and adaptive immune responses in CIA rats.The involvement of TLR4 signalling pathways in the anti-arthritic effect of RL was examined in cartilage tissue of CIA rats,murine RAW264.7macrophages and human THP-1 monocytic cells.RESULTS The severity of arthritis in the CIA rats was significantly attenuated by RL.Histological score and radiographic score were efficiently improved by RL.RL could also dose-dependently inhibit pro-inflammatory cytokines in serum of CIA rats.RL significantly inhibited the production of various pro-inflammatory mediators,the expression and/or activity of the components of TLR4 signalling pathways in animal tissue and cell lines.CONCLUSION RL possesses anti-arthritic effect on collagen-induced arthritis in rats.The therapeutic effect of RL may be related to its inhibition on pro-inflammatory cytokines in serum.The inhibition of the TLR4/TAK1/NF-κB and TLR4/TAK1/MAPK pathways participate in the anti-arthritic effects of RL.This provides a pharmacological justification for the use of RL in the control of various arthritic diseases.Further investigation should be done to develop RL into a modern anti-arthritic agent.展开更多
基金Supported by Bureau of Science and Technology of Huizhou Municipality(2022CQ0100252021SC040202004).
文摘[Objectives]This study was conducted to increase the reproduction coefficient of Japanese honeysuckle(Lonicera japonica)to keep the character of optimal benign.[Methods]The young leaves of medicinal Japanese honeysuckle were selected as explants,and MS was used as the basic culture medium.Suitable culture concentrations and conditions were screened through different concentration gradients of growth regulators and cytokinin.[Results]As the concentration of 6-BA in the culture medium increased,the browning rate increased,and the browning phenomenon occurred earlier.On the contrary,a lower concentration of 6-BA was suitable for the differentiation and growth of young leaves,and the browning response was slow.However,if the cultivation time was too long and the materials were not transferred in a timely manner,browning would also occur.The optimal combination of levels was obtained through a 3×3 orthogonal experiment(three parallel groups for each of 6-BA and NAA).The culture conditions included a constant temperature of 26℃and light intensity of 1200 lx.The optimal medium for inducing callus proliferation was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L;and the optimal medium for inducing bud differentiation was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.[Conclusions]This study provides a theoretical basis for accelerating the development of the honeysuckle industry.
基金National Traditional Chinese Medicine Characteristic Technology Heritage Talent Training Program(T20184828005,2015481601003).
文摘[Objectives]To optimize the extraction process of total flavonoids of Lonicerae japonica and establish a method for its extraction and content determination.[Methods]The total flavonoids of L.japonica were extracted by reflux extraction method.Through a single factor experiment,the effects of extraction method,extraction solvent concentration,extraction volume and extraction time on the total flavonoids content of medicinal materials were investigated to select the optimal extraction process of the total flavonoids of L.japonica.[Results]The optimal extraction process of the total flavonoids of L.japonica was 70%ethanol,1∶30 of solid-to-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]This method can effectively determine the content of total flavonoids in L.japonica and is expected to provide a certain scientific basis for the study of the quality standard of L.japonica.This method has high reproducibility.It is stable and feasible in extraction of total flavonoids from L.japonica.
基金This study was supported by 2016 National College Students Innovation and Entrepreneurship Training Project(201610599001)Guangxi Key R&D Project(Gui Ke AB18221095)Youjiang Medical College for Nationalities High-level Talent Research Project(01002018079).
文摘Background:To study the protective effect of different medicinal parts of honeysuckle on myocardial ischemia injury in rats.Method:SD mice were randomly divided into 6 groups:the normal group,model group,compound salvia miltiorrhiza group,Lonicera japonica Thunb.of flower bud group,flower group,and the stem leaf group.There were 8 rats in each group,including 4 males and 4 females.The normal group and model group were given equal amount of distilled water,whereas the other dosage groups were given the appropriate drug by intragastric administration,continuous gavage for 14 days.From the 12 days,in addition to the normal group were injected of the same amount of normal saline,the other groups were injected with 5g/kg.Injection of isoproterenol to establish the mouce mycardial ischemia model,its continuously 3d.To detect the serum of AST,CK,CK-MB,LDH and the myocardial issue of Chinese super oxideismutase and malondialdehyde level.Results:Compared with normal group,the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde were significantly increased in the model group(P<0.01)and the level of super oxideismutase decreased significantly(P<0.01)the levels of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde in the serum of flower bud group,flower group and stem leaf group were significantly decreased(P<0.05),and the decreasing level of flower bud group,flower,leaf group of AST,CK,CK-MB,cTn-I,LDH and malondialdehyde was flower bud group>flower>leaf group,the activity of super oxideismutase was obviously increased,and the activity level of super oxideismutase of flower bud group,flower group and stem leaf group was flower bud group>flower>leaf group.Conclusion:In the myocardial ischemic injury model of rats induced by isoproterenol,Lonicera japonica Thunb.had a protective effect in all parts,and Lonicera japonica Thunb.bud had the greatest protective effect.
基金Laboratory of PhytochemistryKunming institute of BotanyChinese Academy of Sciences.
文摘A new triterpenoid saponin, together with a known saponin, was isolated from the flowers of Lonicera Japonica Thunb. Using chemical and spectroscopic methods. mainly 2D NMR technique. their structures were deduced to be 3-β-D-glucopyranosyl-(1-4)-β-D-glucopyranosyl- (1 - 3)-a-L-rhamnospyranosyl-(1 - 2)-a-L-arabinopyranosyl hederagenin 28-O-β-D-glucopyranosyl- (1 - 6)-β-D-glucopyranoside and 3-a-L-rhamnospyranosyl-(1 - 2)-a- Larabinopyranosyl hederagenin 28-O-a-L-rhamnospyranosyl - (1 - 2 )- [β - D- xylcopyranosyl-(1 - 6)]-β-D-glucopyranoside. respectively.
基金supported by Fundamental Research Grant Scheme(FRGS/1/2014/SG01/UNIM/02/1)Ministry of Higher Education Malaysia and University of Malaya Research Grant(UMRG 544/14HTM and UMRG 362-15AFR)
文摘Objective:To examine the acanthamoebicidal effects of ethyl acetate,aqueous and butanol fractions of dried flower buds of Lonicera japonica(L.japonica) Thunb.(Flos Lonicerae) in vitro.Methods:Acanthamoeba triangularis isolates were obtained from environmental water samples and identified by PCR.They were exposed to ethyl acetate,water and butanol fractions of L.japonica Thunb.at concentrations ranging from 0.5 mg/m L to 1.5 mg/m L.The extracts were evaluated for growth inhibition at 24,48 and 72 h,respectively.Chlorogenic acid at a concentration of 1 mg/mL was examined for inhibition of encystment.Results:Ethyl acetate fraction at a concentration of 1.5 mg/mL evoked a significant reduction of trophozoite viability by 48.9% after 24 h,49.2% after 48 h and 33.7% after 72 h Chlorogenic acid,the major active constituent of L.japonica Thunb.at the concentration of 1 mg/mL reduced the cysts/trophozoite ratio by 100% after 24 h,84.0% after 48 h and 72.3% after 72 h.This phenolic compound at concentration of 1 mg/mL concurrent with 0.6% hydrogen peroxide inhibited hydrogen peroxide-induced encystment by 92.8% at 72 h.Conclusions:Results obtained from this study show that ethyl acetate fraction at 1.5 mg/m L is the most potent fraction of L.japonica Thunb.and its major constituent chlorogenic acid showed the remarkable inhibition of encystment at a concentration of 1 mg/mL.
基金Supported by Science and Technology Planning Project of Tianjin(17JCYBJC29800&16PTZSTG00020)Science and Technology Planning Project of Jinnan District of Tianjin(201802055)Science and Technology Planning Project of Binhai New Area of Tianjin(BHXQKJXM-SF-2018-33)
文摘Lonicera japonica Thunb. is a typical medicinal and edible plant in China. It has antibacterial, anti-oxidant, anti-endotoxin, and anti-inflammatory effects, etc. Its pharmacological action is closely related to its active components. Based on the results of previous studies, this paper summarized the effects of variety, origin, cultivation conditions, pruning, harvesting period and processing methods on the active components of L. japonica , in the hope of providing theoretical guidance for high-yield and high-quality cultivation of L. japonica.
基金Grants from the National Natural Science Foundation of China, No. 39600061National Administration of Traditional Chinese Medicine, No.2003LHR13+3 种基金Medical Science Foundation of Guangdong Province, No. A2004327, A2006334the Natural Science Foundation of Guangdong, No. 04010443, 06105246 Administration Bureau of Traditional Chinese Medicine of Guangdong Province, No.1040074Technology Bureau of Guangzhou, No. 2007J1-C0041
文摘BACKGROUND: It has been shown that interleukin-1β (IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2,which binds with receptors of the thermo-sensitive hypothalamic neurons. Lonicera japonica is one of the medicinal plants used widely in Asia for its antipyretic properties. However,these mechanisms have not yet been intensively studied. OBJECTIVE: To investigate the antipyretic effect and mechanisms of Lonicera japonica on IL-1β-induced febrile New Zealand rabbits by observing expression changes of E-type prostaglandin receptor-3 (EP3) mRNA in the preoptic anterior hypothalamus (POAH). DESIGN: A randomized controlled study. SETTING: Electrophysiological Laboratory at the Department of Pathophysiology,Medical College of Jinan University; Department of Orthopaedics,First Hospital Affiliated to Medical College of Jinan University. MATERIALS: The experiment was performed from April to December 2005,using a total of 32 New Zealand white rabbits of both sexes,weighing 1.5-2.0 kg. All the animal experiments were performed according to the internationally accepted ethical guidelines. Lonicera japonica injection was purchased from Huanghe pharmaceutical factory of Xi'an,China. IL-1β was purchased from Sigma,USA. METHODS: A total of 32 rabbits were divided randomly into four groups: ① Normal saline (NS) control group; ② Lonicera japonica treatment group; ③ IL-1β treatment group; and ④ Lonicera japonica plus IL-1β treatment group. In the first 3 groups,the rabbits were given separate intravenous (i.v.) injections of 1 mL NS,1 mL Lonicera japonica,and 100 ng IL-1β (dissolved in 0.9% NS without pyrogen). In the Lonicera japonica plus IL-1β group each rabbit was given i.v. injections of 1 mL NS and,30 minutes later,100 ng IL-1β. MAIN OUTCOME MEASURES: Colonic temperature of each rabbit was measured at 0,10,20,30,40,50,60,and 70 minutes after injection and the maximum temperature rise (ΔT) and the temperature response index after 1 hour (TRI1) was calculated. Subsequently,in situ hybridization (ISH) was done with an ISH kit,EP3 mRNA expression in the POAH of all groups was measured (number of positive cells and average gray scale value). RESULTS: A total of 32 rabbits were included in the result analysis,without any loss. ①ΔT and TRI1: there was no significant difference between the Lonicera japonica group and NS group (P > 0.05). The IL-1β group was significantly greater compared to NS group (P < 0.01). The Lonicera japonica plus IL-1β group was significantly less than the IL-1β group (P < 0.05). ② In the NS and Lonicera japonica groups,the EP3 mRNA expression was negative (no coloration) or only weakly positive (only a few brown yellow particles in the cytoplasm cells could not be identified). The number of positive cells and the average gray scale value were not significantly different between the two groups (P > 0.05). In the IL-1β group,the number of positive cells was remarkably higher and the average gray scale value was lower than the NS group (P < 0.01). In the Lonicera japonica plus IL-1β group,the number of positive cells was significantly less than the IL-1β group (P < 0.05). However,the average gray scale value was greater than the IL-1β group (P < 0.05). CONCLUSION: Lonicera japonica has obvious antipyretic effects on IL-1β-induced febrile rabbits and acts by inhibiting expression of EP3 mRNA in the POAH.
基金supported by National Nature Science Foundation of China (81973284)Scientific Research Foundation of the Education Department of Liaoning Province (LJKZ0944)Jiangsu Province Capability Improvement Project through Science,Technology and Education (ZDXYS202207).
文摘Lonicera japonica,also known as honeysuckle,is an evergreen shrub in the family of Syzygium.By consulting Sciencedirect databases and Web of Science databases,79 related articles were found,of which 22 were related to chemical composition and pharmacological activity.These articles show that L.japonica has a wide range of pharmacological activities,including antiviral,anti-tumor,and antioxidant effects.These activities have important applications in the pharmaceutical,food,and fragrance industries.This review focuses on the chemical composition and pharmacological effects of L.japonicae,which is of great significance to the development of new drugs and therapeutic methods.
基金financially supported by the National Natural Science Foundation of China (81973604, 81803690 and 81703684)Special Funds from the Central Finance to Support the Development of Local Universities+12 种基金the National Natural Science Foundation Matching Project (2018PT02)the Innovative Talents Funding of Heilongjiang University of Chinese Medicine (2018RCD25)the Postdoctoral Initial Fund of Heilongjiang Province (UNPYSCT 2017219)the University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2017215)the National Natural Science Foundation Matching Project (2017PT01)the Natural Science Foundation of Heilongjiang Province (H2015037)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2014bs05)the Application Technology Research and Development Projects of Harbin Technology Bureau (2014RFQXJ149)the Heilongjiang Postdoctoral Scientific Research Developmental Fund (LBH-Q16210 and LBH-Q17161)the Heilongjiang University of Chinese Medicine Doctoral Innovation Foundation (2013bs04)the scientific research project of Heilongjiang Provincial Health Commission (20211313050171)Heilongjiang Touyan Innovation Team ProgramNational Famous Old Traditional Chinese Medecine Experts Inheritance Studio Construction Program of National Administration of TCM ([2022]No.75)
文摘Lonicerae japonicae Flos(LF)is a kind of healthcare food with hepatoprotective function.This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF.Fingerprints of ten batches of LF were established by UPLC-PDA.The inhibitory levels of AST and ALT were used as pharmacological indexes,and secoxyloganin,isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis(GRA)and partial least squares regression analysis(PLSR).Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds.Molecular docking is used to explore the interaction between small molecules and proteins.This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF,which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.
基金Financial support from the National Natural Science Foundation of China (NNSFC,Nos.20772156 and 30825044)the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT,No.IRT1007)the National Science and Technology Project of China (No.2012ZX09301002-002)
文摘Three new glucosylated caffeoylquinic acid isomers(1–3),along with six known compounds,have been isolated from an aqueous extract of the flower buds of Lonicera japonica.Structures of the new compounds were determined by spectroscopic and chemical methods as()-4-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(1),()-3-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(2),and()-5-O-(4-O-β-D-glucopyranosylcaffeoyl)quinic acid(3),respectively.In the preliminary in vitro assays,two known compounds methyl caffeate and 2?-O-methyladenosine showed inhibitory activity against Coxsackie virus B3 with IC50 values of 3.70 μmol/L and 6.41 μmol/L and SI values of 7.8 and 12.1,respectively.
基金supported the National Natural Science Foundation of China(No.81073069)
文摘The anti-bacterial activities of three types of di-O-caffeoylquinic acids(diCQAs) in Lonicera japonica flowers, a traditional Chinese medicine(TCM), on Bacillus shigae growth were investigated and compared by microcalorimetry. The three types of diCQAs were 3, 4-di-O-caffeoylquinic acid(3, 4-diCQA), 3, 5-di-O-caffeoylquinic acid(3, 5-diCQA), and 4, 5-di-O-caffeoylquinic acid(4, 5-diCQA). Some qualitative and quantitative information of the effects of the three diCQAs on metabolic power–time curves, growth rate constant k, maximum heat-output power Pm, and the generation time tG, total heat output Qt, and growth inhibitory ratio I of B. shigae were calculated. In accordance with a thermo-kinetic model, the corresponding quantitative relationships of k, Pm, Qt, I and c were established. Also, the half-inhibitory concentrations of the drugs(IC50) were obtained by quantitative analysis. Based on the quantity–activity relationships and the IC50 values, the sequence of inhibitory activity was 3, 5-diCQA > 4, 5-diCQA > 3, 4-diCQA. The results illustrate the possibility that the caffeoyl ester group at C-5 is the principal group that has a higher affinity for the bacterial cell, and that the intramolecular distance of the two caffeoyl ester groups also has an important influence on the anti-bacterial activities of the diCQAs.
基金This work was supported by the Ph.D.Startup Foundation of Guizhou University of Traditional Chinese Medicine(no.(2020)32 and no.(2019)141)National Natural Science Foundation of China(no.31970629).
文摘Lonicera japonica Thunb.,a traditional Chinese herb,has been used for treating human diseases for thousands of years.Recently,the genome of L.japonica has been decoded,providing valuable information for research into gene function.However,no comprehensive database for gene functional analysis and mining is available for L.japonica.We therefore constructed LjaFGD(www.gzybioinformatics.cn/LjaFGD and bioinformatics.cau.edu.cn/LjaFGD),a database for analyzing and comparing gene function in L.japonica.We constructed a gene co-expression network based on 77 RNA-seq samples,and then annotated genes of L.japonica by alignment against protein sequences from public databases.We also introduced several tools for gene functional analysis,including Blast,motif analysis,gene set enrichment analysis,heatmap analysis,and JBrowse.Our co-expression network revealed that MYB and WRKY transcription factor family genes were co-expressed with genes encoding key enzymes in the biosynthesis of chlorogenic acid and luteolin in L.japonica.We used flavonol synthase 1(LjFLS1)as an example to show the reliability and applicability of our database.LjaFGD and its various associated tools will provide researchers with an accessible platform for retrieving functional information on L.japonica genes to further biological discovery.
基金supported by the Standardization Project Fund of Administration of Traditional Chinese Medicine(No.ZYBZH-Y-SD-32)the Specialized Fund for Dependent Innovation of Shandong Province(No.2013CXC20401)
文摘Three new secoiridoid glycosides,named lonijapoglycol A(1),aldosecolohanin C(2)and aldosecolohanin B(3),together with three known ones(4–6),have been isolated from the flower the buds of Lonicera japonica.All the structures were identified by spectroscopic analyses.Lonijapoglycol A(1)expressed significant anti-inflammatory activity to inhibit the release ofβ-glucuronidase induced by platelet-activating factor in rat polymorphonuclear leukocytes with an IC50 value of 3.76μmol·L^-1.
基金Financial support from the National Natural Science Foundation of China(NNSFCNos.20772156 and 30825044)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT,No.IRT1007)the National Science and Technology Project of China(Nos.2012ZX09301002-002 and 2011ZX0 9307002-01) is acknowledged
文摘Two new homosecoiridoids,named loniaceticiridoside(l)and lonimalondialiridoside(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures including the absolute configuration were determined by extensive spectroscopic studies,especially by 2D NMR and CD data analysis.A proposed biosynthetic pathway and preliminary investigations of the biological activity of compounds 1 and 2 are also discussed.
基金Financial support from the National Natural Science Foundation of China(NNSFCNos.20772156 and 30825044)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT,No.IRT1007)the National Science and Technology Project of China(Nos.2012ZX09301002-002 and 2011ZX0 9307-002-01)
文摘Two new l-(6'-O-acyl-β-D-glucopyranosyl)pyridinium-3-carboxylates,lonijaponinicotinosides A(1)and B(2),were isolated from an aqueous extract of the flower buds of Lonicera japonica.Their structures were determined by spectroscopic data analysis,and confirmed by comparison with synthetic 1-β-Dglucopyranosylpyridinium-3-carboxylate.
基金Supported by National Science and Technology Major New Drugs Project of China:Study on the allergic markers and early warning system of Shuanghuanglian Injection,(No.2014ZX09201022-006)
文摘OBJECTIVE: To assess the antioxidant capacity of aqueous extracts of buds and flowers of Lonicera japonica Thunb.(BLJ and FLJ) using in vitro assays.METHODS: We assessed the in vitro antioxidant activities of aqueous extracts of BLJ and FLJ and compared with that of classical antioxidants vitamin C and butylated hydroxytoluene, using several well-established methods including the 1,1-diphenyl-2-picryl-hydrazyl assay, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay, reducing power assay, fluorescence recovery after photobleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: The aqueous extracts of both BLJ and FLJ had similarly potent antioxidant capacity. There were no significance differences between BLJ and FLJ in all the assays.CONCLUSION: The aqueous extracts of both BLJ and FLJ have antioxidant activity with comparable efficacy. These findings suggest that both BLJ and FLJ may have the potential as natural antioxidants.
文摘Lonicerae japonicaeFlos (LJF) is widely used in traditional Chinese medicines for the treatment of various diseases, which is now in great demand every year and has a broad development prospect. However, the flowering phase of common LJF varieties is so short, which seriously restricts the development of LJF industry. As a new cultivated kind of Lonicerae japonicae Flos, “Hua Jin 6” has characteristics in long flowering phase and conveniently picking, which makes it have a broad development prospect. The aim of this study is to provide scientific guidance for its suitable harvest period by measuring yield and quality of “Hua Jin 6” from different harvest time. Studies show that flower size had a slowly rising trend from the first day to the seventh day, and then slowly declined or kept stable. There were no significant differences of total phenolic acid contents in different samples from different days, but contents of total flavonoids were on the rise and up to maximum in the ninth day. The contents of total iridoids had an increasing tendency from the first day to the fifth day and then kept relatively stable in other days. We demonstrated that the quality of “Hua Jin 6” is relatively stable and suitable for harvesting in all flower buds white stage in term of HPLC fingerprints. Our findings can make it possible to select the suitable time for different harvest purpose.
基金The project supported by Science,Technology and Innovation Commission of Shenzhen(JCYJ20120829154222473and JCYJ20140807091945050)Research Grants Council of Hong Kong(HKBU 262512)+1 种基金Food and Health Bureau of Hong Kong(HMRF 11122521)Hong Kong Baptist University(FRG2/14-15/056,FRG1/14-15/061)
文摘OBJECTIVE Rheumatoid arthritis(RA)is the most common inflammatory autoimmune disease,affecting around 1% of the world population.Toll-like receptor 4(TLR4)signalling has been found to be involved in the pathogenesis of RA.It is a potential therapeutic target for RA treatment.A herbal formula(RL)consisting of Rosae Multiflorae Fructus and Lonicerae Japonicae Flos has traditionally been used in treating various inflammatory disorders.In this study,we would evaluate the anti-arthritic effect of RL on collagen-induced arthritis(CIA)in rats and investigate the involvement of TLR4 signaling in the mode of action of RL in vivo and in vitro.METHODS In vivo anti-arthritic efficacy was evaluated using CIA rats induced by bovine typeⅡ collagen.The treatment groups were treated with various concentrations of RL or positive control indomethacin for 35 d.Clinical signs(hind paw volume and arthritis severity scores),changes in serum inflammatory mediators,histological and radiographic changes of joints were investigated.Spleens and peritoneal macrophages were used to determine the effects of RL on innate and adaptive immune responses in CIA rats.The involvement of TLR4 signalling pathways in the anti-arthritic effect of RL was examined in cartilage tissue of CIA rats,murine RAW264.7macrophages and human THP-1 monocytic cells.RESULTS The severity of arthritis in the CIA rats was significantly attenuated by RL.Histological score and radiographic score were efficiently improved by RL.RL could also dose-dependently inhibit pro-inflammatory cytokines in serum of CIA rats.RL significantly inhibited the production of various pro-inflammatory mediators,the expression and/or activity of the components of TLR4 signalling pathways in animal tissue and cell lines.CONCLUSION RL possesses anti-arthritic effect on collagen-induced arthritis in rats.The therapeutic effect of RL may be related to its inhibition on pro-inflammatory cytokines in serum.The inhibition of the TLR4/TAK1/NF-κB and TLR4/TAK1/MAPK pathways participate in the anti-arthritic effects of RL.This provides a pharmacological justification for the use of RL in the control of various arthritic diseases.Further investigation should be done to develop RL into a modern anti-arthritic agent.
