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响应面法优化Rhizopus microsporus var.chinensis产生淀粉酶的条件 被引量:2
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作者 李彧娜 石贵阳 +1 位作者 王武 王正祥 《食品工业科技》 CAS CSCD 北大核心 2010年第11期184-186,共3页
对Rhizopus microsporus var.chinensis固态发酵产生淀粉酶的条件进行了优化。首先采用单因子实验确定最适固态发酵基质为小麦麸皮,最适碳源和氮源分别为可溶性淀粉和硫酸铵,通过控制培养基初始pH为3.0、初始湿度为70%时,使其生淀粉酶... 对Rhizopus microsporus var.chinensis固态发酵产生淀粉酶的条件进行了优化。首先采用单因子实验确定最适固态发酵基质为小麦麸皮,最适碳源和氮源分别为可溶性淀粉和硫酸铵,通过控制培养基初始pH为3.0、初始湿度为70%时,使其生淀粉酶产量提高3.5倍。在此基础上,利用响应面中心组合设计对显著因素进行优化,得出每500mL三角摇瓶中含小麦麸皮13.7g、可溶性淀粉0.063g、硫酸铵0.052g时,生淀粉酶产量达到48.50U/mL,比初始产量提高了8倍。 展开更多
关键词 生淀粉酶 响应面优化 RHIZOPUS microsporus var.chinensis
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崇明水仙(Narcissus tazetta var. chinensis)不同外植体分化能力与鳞茎增大初探 被引量:10
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作者 熊莉君 李小方 +2 位作者 王洋 孙越 许玲 《华东师范大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第6期103-109,共7页
以崇明水仙鳞茎的上、中、下部、嫩叶和幼嫩花序为外植体,研究其在离体培养系统中的再分化能力,结果表明,带鳞茎盘的鳞茎和幼嫩花序最容易分化出小鳞茎.以组培获得的带鳞茎盘的鳞茎为材料建立快繁体系,40d内小鳞茎数目增加3倍;并发现浓... 以崇明水仙鳞茎的上、中、下部、嫩叶和幼嫩花序为外植体,研究其在离体培养系统中的再分化能力,结果表明,带鳞茎盘的鳞茎和幼嫩花序最容易分化出小鳞茎.以组培获得的带鳞茎盘的鳞茎为材料建立快繁体系,40d内小鳞茎数目增加3倍;并发现浓度适合的KH2PO4及暗培养能促进小鳞茎增大.组织学研究结果显示,组培水仙与大田生长水仙的顶端分生组织的形态结构不同,前者细胞分层不明显,细胞核大且染色较深. 展开更多
关键词 崇明水仙 外植体 分化能力 鳞茎增大
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青菜(Brassica campestris spp.chinensis var.)TPS雄性不育系的转育 被引量:8
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作者 李树林 周熙荣 +1 位作者 李清芳 周志疆 《上海农业学报》 CSCD 1998年第1期9-12,共4页
在甘蓝型油菜中发现一个非常优良的不育源─—TPS雄性不育系,本研究通过远缘杂交和连续回交等手段,把TPS不育胞质从甘蓝型油菜转入青菜,选育出3个回交五代的青菜TPS不育系即青1A;青2A;青3A。不育系的不育性彻底而稳定,没有发现任... 在甘蓝型油菜中发现一个非常优良的不育源─—TPS雄性不育系,本研究通过远缘杂交和连续回交等手段,把TPS不育胞质从甘蓝型油菜转入青菜,选育出3个回交五代的青菜TPS不育系即青1A;青2A;青3A。不育系的不育性彻底而稳定,没有发现任何低温黄化、蜜腺退化和花器畸形等不良的细胞质负效应,群体基本稳定一致,结实性状良好。现已进入杂交组合测配阶段。 展开更多
关键词 青菜 质核互作型 雄性不育系 杂种优势 转育
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Anatomy, Histochemistry and Phytochemistry of Leaves in Aloe vera var. chinensis 被引量:5
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作者 沈宗根 Elena CHAUSER-VOLFSON +1 位作者 Yitzchak GUTTERMAN 胡正海 《Acta Botanica Sinica》 CSCD 2001年第8期780-787,共8页
A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.)... A multidisciplinary approach-anatomy, histochemistry and phytochemistry-was used to investigate the leaf structure, the content and the storage location of barbaloin in the leaves of Aloe vera L. var. chinensis (Haw.) Berg. Xeromorphic characteristics including secondary thickened epidermal cell walls, thicker cuticle, ambiguous differentiation of spongy and palisade tissues in the chlorenchyma, and well-developed aquiferous tissue could be seen in the leaves. Several large parenchymatous cells were observed at the phloem pole of the first ring of vascular bundles. The secondary ring of vascular bundles in the leaf base and the stomata, which are surrounded by five cells, have some classification significance in this species. The density of vascular bundles, the content of barbaloin and the intensity of histochemical reaction differed among leaf numbers Ll (annual leaf), L2 (biennial leaf), L3 (triennial leaf) and L4 (quadrennial leaf), and in different parts of the leaf. These three factors were highest in the youngest leaf, Ll, and top parts of all the leaves and lowest in the basal parts and the oldest leaf, L4. The density of vascular bundles had a positive correlation to the content of barbaloin. The histochemical results revealed that the small sheath cells that surrounded the bundles might be the location of barbaloin synthesis and the large parenchymatous cells beneath the sheath might be the storage places of this metabolite. 展开更多
关键词 Aloe vera var. chinensis leaf structure barbaloin content density of vascular bundles histochemical reaction
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利用Native制备电泳分离来源于Rhizopus microsporus var. chinensis的葡萄糖淀粉酶同工酶 被引量:1
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作者 李彧娜 石贵阳 +1 位作者 王武 王正祥 《食品与发酵工业》 CAS CSCD 北大核心 2010年第1期1-4,共4页
将Native制备电泳应用于Rhizopus microsporus var. chinensis中2个葡萄糖淀粉酶同工酶的分离,考察了电泳缓冲系统、凝胶浓度和凝胶长度对分离效果的影响。结果表明:先使用Ornstein-Davis系统分离同工酶,再使用0.02mol/L,pH6.2的NaAc.CH... 将Native制备电泳应用于Rhizopus microsporus var. chinensis中2个葡萄糖淀粉酶同工酶的分离,考察了电泳缓冲系统、凝胶浓度和凝胶长度对分离效果的影响。结果表明:先使用Ornstein-Davis系统分离同工酶,再使用0.02mol/L,pH6.2的NaAc.CH3COOH缓冲液洗脱,在长度为6cm的7%的Native电泳胶上,2个性质相近仅电泳迁移率略有差别的同工酶能有效分离。分离回收的蛋白质可保持其活力,便于后续研究。 展开更多
关键词 制备电泳 RHIZOPUS microsporus var.chinensis 葡萄糖淀粉酶 同工酶
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Studies on Triterpenoid Saponins of Seeds of Aesculus chinensis Bunge var. chekiangensis (Hu et Fang) Fang 被引量:2
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作者 郭杰 杨秀伟 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第2期87-91,共5页
Aim To study the saponin constituents of the seeds of Aesculus chinensis Bunge var. chekiangensis (Hu et Fang) Fang. Methods Compounds were separated and purified by macroreticular resin column chromatography and high... Aim To study the saponin constituents of the seeds of Aesculus chinensis Bunge var. chekiangensis (Hu et Fang) Fang. Methods Compounds were separated and purified by macroreticular resin column chromatography and high-performance liquid chromatography. Their structures were elucidated by spectroscopic and hydrolysis analysis. Results Six compounds were isolated from the 70% ethanolic extracts. They were identified as escins IVc, IVd, Ia, Ib, isoescins Ia and Ib, respectively. Conclusion The above compounds were obtained from the seeds of Aesculus chinensis Bunge var.chekiangensis (Hu et Fang) Fang for the first time. 展开更多
关键词 Aesculus chinensis Bunge var. chekiangensis TRITERPENOIDS ESCIN isoescin
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南方红豆杉(Taxus Chinensis Var.Mairei)叶片膜保护系统与温度的关系 被引量:8
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作者 谢吉容 《四川师范大学学报(自然科学版)》 CAS CSCD 2002年第2期194-197,共4页
以缙云山麓的南方红豆杉为材料 ,研究了其叶片低温半致死温度及膜保护系统随气温下降的变化 .结果表明 ,低温半致死温度降低的同时 ,SOD、POX活性增强 ,VC 含量增加 ,使膜保护系统清除活性氧的能力加强 ,O2· - 水平降低 ,MDA含量下... 以缙云山麓的南方红豆杉为材料 ,研究了其叶片低温半致死温度及膜保护系统随气温下降的变化 .结果表明 ,低温半致死温度降低的同时 ,SOD、POX活性增强 ,VC 含量增加 ,使膜保护系统清除活性氧的能力加强 ,O2· - 水平降低 ,MDA含量下降 。 展开更多
关键词 南方红豆杉 半致死温度 膜保护系统 抗寒性 叶片 低温适应机理 SOD活性 POX活性
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Determination of Barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller by HPLC
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作者 高钧 戴荣华 +3 位作者 王玺 隋红 李康 毕开顺 《Journal of Chinese Pharmaceutical Sciences》 CAS 2002年第2期22-25,共4页
A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents o... A reserved-phase HPLC method was developed for the determination of barbaloin in Aloe vera L. var. chinensis (Haw.) Berger and Aloe barbadensis Miller, and whether there was a close relationship between the contents of barbaloin and their environments in which they were growing was decided. A Hypersil ODS column (4.6 mm×200 mm, 5 μm)was used with a mobile phase of methanol-water (40:60, containing 0.1% acetic acid), the flow rate being 1.0 mL·min -1, detection wavelength at 359 nm, and the column temperature being 30℃. The linear range of barbaloin was between 0.0726 and 0.726 μg with a correlation coefficient of 0.9998 and the regression equation being Y=1.9202×10 6X-1801.9. Barbaloin was stable in methanol in 48 h and the instrument precision was 1.2% while the method precision was 4.9%. The contents of barbaloin of 12 samples ranged from 6.160 to 319.1 μg·g -1. The method developed was fast and simple with good reproducibility. There was high correlation between the contents of barbaloin and their growing environments. 展开更多
关键词 Barbaloin Aloe vera L. var. chinensis (Haw.) Berger Aloe barbadensis Miller HPLC
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Effect of Combined Use of Boron and Manganese Fertilizers on the Nutritional Quality and Physiological Indices of Brassica campestris L.ssp.chinensis L.var.utilis
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作者 肖伟 刘红利 +1 位作者 梁英 黄长干 《Agricultural Science & Technology》 CAS 2013年第11期1591-1593,1598,共4页
[Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutri... [Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutrient solutions for growing B. campestris by hydroponics, boric acid and manganese sulfate were added at 0.5, 2.5, and 7.5 mg/L respectively. Another treatment without boron and manganese was prepared as the control. Quality and physiological indices of B. campestris in the 10 treatments were measured. [Result] Boron and manganese shared obvious in-teraction in improving the quality and physiological indices of B. campestris. To cul-tivate B. campestris with high quality and strong resistance, the optimum concentra-tions of boron and manganese in the nutrient solution should be 2.5 mg/L boric acid and 2.5-7.5 mg/L manganese sulfate. [Conclusion] The findings wil provide refer-ence for studying effects of trace elements on nutrient composition of vegetables. 展开更多
关键词 Boron and manganese fertilizer Brassica campestris L. ssp. chinensis L.var. utilis Quality Physiological activity
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华重楼(Paris polyphylla Smith var. chinensis)无性繁殖试验研究 被引量:2
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作者 谷海燕 谢孔平 《中国野生植物资源》 2016年第5期86-88,共3页
华重楼为我国一味珍贵的民族药,本文以华重楼根茎为材料开展其无性繁殖试验,研究植物生长调节剂6-BA不同浓度对其潜伏芽萌发、生长的影响。试验结果显示5mg/L的6-BA可促进华重楼切段的繁殖系数,提高其双芽率、多芽率及有根率。
关键词 华重楼 根茎 无性繁殖
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NAA、2,4-D对崇明水仙(Narcissus tazetta var.chinensis)愈伤组织的诱导及再生的不同影响 被引量:9
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作者 方琪 张雪平 +3 位作者 张伟 贾林燕 许静 李小方 《上海交通大学学报(农业科学版)》 2013年第3期9-15,共7页
目前水仙组织培养过程中愈伤组织的诱导是一大难题。本文以带鳞片的鳞茎盘为外植体,研究了2,4-D、NAA 2种类型的生长素对水仙组织培养中愈伤组织诱导的影响。结果表明,高浓度的2,4-D(3.0~4.0mg/L)结合6-BA能诱导无色瘤状愈伤组织,中等... 目前水仙组织培养过程中愈伤组织的诱导是一大难题。本文以带鳞片的鳞茎盘为外植体,研究了2,4-D、NAA 2种类型的生长素对水仙组织培养中愈伤组织诱导的影响。结果表明,高浓度的2,4-D(3.0~4.0mg/L)结合6-BA能诱导无色瘤状愈伤组织,中等浓度的2,4-D(0.5mg/L)有利于芽点的诱导和瘤状愈伤组织的增殖,而较低浓度的2,4-D(0.1mg/L)有利于诱导出小鳞茎,也能促使瘤状愈伤组织再生成苗。而NAA结合6-BA不能诱导愈伤组织的形成。高浓度的NAA不仅诱导未分化的分生组织细胞分裂,也能促进分化的细胞进行分裂,从而使形成白色凸起状结构。另外,培养基中这2种激素互换可以使组织培养过程中形成的愈伤组织或白色突起发生相互转换。这些结果说明,水仙组织培养过程中愈伤组织的诱导和不同形态的器官发生与培养基中生长素种类和浓度关系密切。 展开更多
关键词 中国水仙 愈伤组织 器官发生 2 4-D NAA 鳞茎
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Isolation and characterization of the AGAMOUS homologous gene NTAG in Chinese narcissus (Narcissus tazetta var. chinensis Roem) 被引量:7
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作者 Wang Zheng-ke Gao Jian +1 位作者 Li Lu-bin Peng Zhen-hua 《Forestry Studies in China》 CAS 2006年第1期21-26,共6页
Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus (Narcissus tazetta var. chinensis Roem), its flowers developed at high temperatures and bloomed at... Amaryllidaceae, a monocot plant family, consists of many important ornamental bulb flower species. Chinese narcissus (Narcissus tazetta var. chinensis Roem), its flowers developed at high temperatures and bloomed at lower temperatures during the Chinese Spring Festival, is a traditional Chinese flower with high economic and ornamental value. To study its flower development, a full length cDNA containing MADS box domain from narcissus was isolated by a reverse transcription polymerase chain reaction (RT-PCR) with degenerate oligo-nucleotide primers derived from a conserved MADS- and K-box domain sequence. The 5' and the 3' regions of the gene were amplified using the PCR protocol for the rapid amplification of cDNA ends (RACE). The 690 bp open reading frame encodes 230 amino acid residues. A comparison of the deduced amino acid sequence of NTAG with the sequence of other MADS box proteins showed 91.3% amino acid identities with HAG (Hyacinthus orientalis). Sequence analysis and alignment showed significant similarity with other AG homologues. RNA blot analysis indicated that the narcissus NTAG gene was expressed only in reproductive organs, especially in stamens and carpels. These results indicated that the NTAG gene was an AG homologue and that the AG genes appeared to be structurally and functionally conserved between dicots and monocots. 展开更多
关键词 NTAG (Narcissus tazetta var. chinensis Roem AGAMOUS) cDNA amplified fragment length polymorphism Chinese narcissus
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Optimization of Cell Line Building for Taxus chinensis var. mairei
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作者 袁梦如 沈斌 +1 位作者 孙瑜霞 沈文飚 《Agricultural Science & Technology》 CAS 2010年第7期136-137,163,共3页
[Objective] The paper aims to optimize the abduction and domestication conditions of Taxus chinensis var.Mairei callus.[Method] We compared the efficiencies of callus between different explants and investigated the mu... [Objective] The paper aims to optimize the abduction and domestication conditions of Taxus chinensis var.Mairei callus.[Method] We compared the efficiencies of callus between different explants and investigated the multiplication conditions of callus and suspended cell culture conditions with the buds,young stems and young leaves from T.chinensis var as the explants.[Results] The effect was the best with the bud as the explants; the best way for sterilizing the explants of T.chinensis var mairei was:streptomycin detergent for 2 h + suds for 3 h + 75% alcohol disinfection for 30 s + 10% sodium hypochlorite solution for 25 min + 1‰ mercury chloride for 10 min; the optimum formula of callus subculture was:B5 + 4.0 mg/L NAA + 0.5 mg/L 2,4-D + 0.2 mg/L GA + 0.5 mg/L 6-BA + 2 g/L AC.[Conclusion] This research built the high efficient regeneration system of T.chinensis var. 展开更多
关键词 Taxus chinensis var.mairei CALLUS Successive transfer culture Suspended cell culture
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单瓣月季花Rosa chinensis var.spontanea的发现与历史 被引量:4
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作者 孟静 《园艺与种苗》 CAS 2012年第8期6-7,11,共3页
月季(Rosa hybrida L.)是世界最具经济价值的切花和园艺花卉,作为现代月季最重要的祖先之一——月季花(R.chinensis),一直以来都备受国内外育种家的高度关注。该文通过广泛查阅文献资料,对月季花及其野生型——单瓣月季花(R.chinensis v... 月季(Rosa hybrida L.)是世界最具经济价值的切花和园艺花卉,作为现代月季最重要的祖先之一——月季花(R.chinensis),一直以来都备受国内外育种家的高度关注。该文通过广泛查阅文献资料,对月季花及其野生型——单瓣月季花(R.chinensis var.spontanea Rehder & Wilson)的发现、发表和历史等多方面进行了阐述,拟为国内外月季育种家提供参考,为中国育种事业提供宝贵的理论依据和研究背景。 展开更多
关键词 月季 月季花 单瓣月季花 育种历史
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Effects of Piriformospora indica on the Respiration of Taxus chinensis var.mairei under Water Stress 被引量:3
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作者 Longsheng Hu Xinru He +3 位作者 Pujie Wei Bing Sun Yongjun Fei Die Hu 《Phyton-International Journal of Experimental Botany》 SCIE 2021年第6期1661-1672,共12页
Seedlings of Taxus chinensis var.mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress.The materials were divided into two groups,namely,with or w... Seedlings of Taxus chinensis var.mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress.The materials were divided into two groups,namely,with or without inoculation with P.indica.Each group was subjected to four different levels of water stress.Vitality and physiological and biochemical indexes of the roots of T.chinensis var.mairei were regularly measured.Under water stress,T.chinensis var.mairei had significantly decreased root vitality;root vitality was higher in inoculated roots than in uninoculated roots.Under intense water stress,the inoculated roots had a higher soluble sugar content than the uninoculated roots.Under water stress,T.chinensis var.mairei experienced decreased activity of aerobic respiratory metabolic enzymes.The activity of anaerobic respiratory metabolic enzymes and alcohol dehydrogenase initially increased and then decreased,whereas that of lactate dehydrogenase increased.The inoculated roots had a higher activity of respiratory metabolic enzymes than the uninoculated roots.As water stress was further intensified,the roots had significantly decreased activity of aerobic respiratory metabolic enzymes and significantly increased activity of anaerobic respiratory metabolic enzymes.The activity of respiratory metabolic enzymes decreased faster in the uninoculated roots than in the inoculated roots.This study demonstrated that Piriformospora indica plays a positive role in enhancing the antihypoxic ability of T.chinensis var.mairei,thereby alleviating plant damage due to water stress. 展开更多
关键词 Taxus chinensis var.mairei Piriformospora indica water stress RESPIRATION
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Comprehensive Evaluation on Impacts of Diseases on Quality of Paris polyphylla var. chinensis Based on Ultraviolet and Infrared Spectra 被引量:1
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作者 Yongxia KE Jixiu SHEN +3 位作者 Hairong ZHONG Risha WEIZE Yunzhang XU Yuan LIU 《Medicinal Plant》 CAS 2019年第3期63-65,68,共4页
[Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in roo... [Objectives] The research aimed to evaluate impacts of diseases on quality of Paris polyphylla var. chinensis. [Methods]Ultraviolet spectrophotometry was used to determine and contrast content of total saponins in root,stem and leaf from healthy and diseased plants. Infrared characteristic absorption peaks of healthy and diseased plants were found by infrared fingerprint method for comparative analysis.[Results]Total saponins content in root sample J4 of healthy plant reached 4. 89% and was the highest,while it was 0. 13% in stem sample J11 of healthy plant and was the lowest. Total saponins content in root sample B1 of diseased plant was 1. 68% and was the highest,while it was 0. 1% in stem sample B7 of diseased plant and was the lowest. In healthy and diseased plants,total saponins content in root was significantly higher than that in stem and leaf. Content of total saponins in root from healthy plant was significantly higher than that from diseased plant,and there was little difference in stem and leaf of healthy and diseased plants. Root sample Jg of healthy plant had obvious C-O characteristic vibration of polysaccharides and glycosides and absorption peak of O-H bending vibration in steroidal saponin near 1 160. 02 cm-1,while diseased plant had no obvious absorption peak. Near 861. 97 and 768. 07 cm-1,root sample Jg of healthy plant had significant C-C stretching vibration in sugar ring and characteristic absorption peak of characteristic vibration of steroidal saponin,while root sample Bg of diseased plant had no obvious characteristic peak. [Conclusions] Diseases significantly affected composition and content of total saponins and steroidal saponins from P. polyphylla var. chinensis,further affecting quality of P. polyphylla var. chinensis. 展开更多
关键词 PARIS polyphylla var. chinensis ULTRAVIOLET Infrared Total SAPONINS QUALITY
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Improved Production of Paclitaxel from Suspension Culture of Taxus chinensis var. mairei by in situ Extraction with Organic Solvents 被引量:1
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作者 未作君 元英进 +1 位作者 吴兆亮 吴金川 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2003年第2期224-226,共3页
The production of paclitaxel from suspension culture of Taxus chinensis var,mairei was improved by in situ extraction with organic solvents to avoid feedback repression and product degradation.Oleic acid and dibutyl p... The production of paclitaxel from suspension culture of Taxus chinensis var,mairei was improved by in situ extraction with organic solvents to avoid feedback repression and product degradation.Oleic acid and dibutyl phthalate were proved to be suitable solvents .The optimal volumetric percentage of organic solvents in the culture medium was found to be around 8%,and the favorable time for their introduction was at the exponential phase of cell growth,Paclitaxel production with the in situ extraction was ca 3-fold of that without extraction. 展开更多
关键词 EXTRACTION organic solvent PACLITAXEL Taxus chinensis var. mairei two-liquid-phase culture
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TISSUE CULTURE OF ROSA CHINENSIS VAR.FLORIBUNDA
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作者 朱虹 《Journal of Northeast Forestry University》 SCIE CAS CSCD 1996年第2期41-45,共5页
The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS med... The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS medium with the addition of 6-HA 1 .0 mg/L,. and differentiation was best on MS medium with addition of 6-BA 1 .5 + NAA0.05 + ZT0.1 mg/L, or KT 1,0 + NAA0,05 + ZT0.1mg/L. The MS emdium with addition of 6-BA 0.3 + NAA 0.0 5+ ZT0.1 mg/L, or KT 0.3 + NAA0.05 +ZT0. 1 mg/L showed a good result for developing strong shoots. 1/2 MS medium with the addition ofIBA 0.1 mg/L, or IBA0.1 + NAA0.02 mg/L, had best result for rooting. The plantlets should be transplanted from test-tube to soil when they grew ' to 2.5 ~ 4.0 cm high and have 3 ~ 5 strips short roots. Ahigher survival rate was obtained under the conditions of conterolling humidity and temperature 展开更多
关键词 Rosa chinensis var. Floribund Tissue culture CYTOKININ AUXIN
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Dynamic Effects of Cerium on Syntheses of Soluble Protein and Taxol in Suspension Culture of Taxus Chinensis Var. Mairei Cells
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作者 李景川 马忠海 +2 位作者 元英进 孙安慈 胡昌序 《Journal of Rare Earths》 SCIE EI CAS CSCD 2001年第3期223-228,共6页
The dynamic effects of Ce4+ on the syntheses of soluble protein and taxol in suspension cultures of Taxus chinensis var. mairei cells were studied. The phenomena of 'partition' and 'bifurcation' were o... The dynamic effects of Ce4+ on the syntheses of soluble protein and taxol in suspension cultures of Taxus chinensis var. mairei cells were studied. The phenomena of 'partition' and 'bifurcation' were observed in studying the dynamic effect of Ce4+ on soluble protein synthesis and cell activity. That is, Ce4+ of low concentration improves the soluble protein synthetic strength and cell activity, while Ce4+ of high concentration is harmful to protein synthesis and cell activity. In addition, Ce4+ of appropriate concentration enhances taxol synthesis. 展开更多
关键词 rare earths CERIUM soluble protein TAXOL plant cell culture Taxus chinensis var. mairei
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Effects of Stratification and Hormone Treatments on Germination and Physio-Biochemical Properties of <i>Taxus chinensis var. mairei</i>Seed
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作者 Yanjie Zhang Shunbao Lu Handong Gao 《American Journal of Plant Sciences》 2012年第7期829-835,共7页
In order to learn dormancy mechanism of Taxtts chinensis var. mairei seed, effects of stratification and hormone treatments on physiological and biochemical indexes of the seeds after stratifying for eight months were... In order to learn dormancy mechanism of Taxtts chinensis var. mairei seed, effects of stratification and hormone treatments on physiological and biochemical indexes of the seeds after stratifying for eight months were researched. The content of amylase activity and soluble sugar gradually increased at first stratification, and then to the maximum for the end of after-ripening period. With the dormancy of seed relieving, some soluble sugar of endosperm was used or consumed by respiration and that led to their content decreased. Seed soluble protein was gradually accumulated at the beginning of stratification and then the initial raw material of protein synthesis of embryo during development had been used or in part converted into carbohydrates for energy consumption, so that the soluble protein content decreased. The fat and starch content slowly decreased during stratification, which showed that the storage of material had continually carrying out catabolism and conversion, which might provide energy sources for seed germination in the stratification process. The peroxidase activity of Taxus seeds increased gradually at the beginning of stratification, and then reached maximum point during stratification for 4 months in A1 and A2. The G-6-PDH and 6-PGDH joint activity was rapidly increased to reach the maximum during stratification for 4 months in A1, which showed the pentose phosphate pathway was activated at this time. The peroxidase activity rapidly increased might also be related to respiratory metabolic pathway during stratification. In plants, acid phosphatase hydrolyze PAM metabolites, namely phosphate material was related to energy metabolism, especially to high-energy phosphate, and phosphate enzyme activity changes affected the energy metabolism. The acid phosphatase activity of Taxus seeds in different treatments has no significant changes during stratification process, but there were two maximums. Energy metabolism might get benefit from the increase of acid phosphatase activity. There were significant differences in amylase activity, peroxidase activity, soluble sugar and soluble protein content with different treatments. Effect of promoting seed germination soaked by GA before stratification was obvious, but the concentration of GA should not be too high. The high concentration of GA caused seed to rot and reduce the seed germination rate. In this study, the highest germination rate of seed was 71.8% and soaked in 200 mg·L-1 gibberellin. 展开更多
关键词 Taxtts chinensis var. mairei SEED Stratification Hormone Physiology and BIOCHEMISTRY
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