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Relationship between ethylene and the occur-rence of cytoplasmic malesterility in rice 被引量:1
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《Chinese Rice Research Newsletter》 2000年第1期9-9,共1页
关键词 Relationship between ethylene and the occur-rence of cytoplasmic malesterility in rice
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Identification of Nucleus Sterility Candidate Genes Using a Resequencing Technique in Sweet Pepper Sterile Line AB91 被引量:1
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作者 Yaning Meng Libin Yan +1 位作者 Hongxiao Zhang Yanqin Fan 《Agricultural Sciences》 2021年第2期138-157,共20页
Breeding hybrids with nuclear malesterile lines is an important method for the cross-breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper. Yet, there ar... Breeding hybrids with nuclear malesterile lines is an important method for the cross-breeding of sweet peppers. To date, few reports have been published on the nuclear malesterility gene of sweet pepper. Yet, there are approximately 20 pepper nuclear malesterility lines in the world. Using the self-developed testing material, sweet pepper nuclear malesterile dual-purpose line AB91, the genome-wide resequencing technique was applied to find that the mutation site causing the abortion of sweet pepper nuclear malesterility AB91 is on chromosome #5. The mutation gene Capana05g000747 was filtered out and validated by the flight mass spectrometry genotyping and quantitative realtime PCR method and determined to be the gene causing the abortion of sweet pepper nuclear male sterility AB91. The gene Capana05g000747 mutation site is a non-synonymous mutation site located at the 6th exon, the base C mutated into A, and the amino acid changed from alanine to serine. The three-dimensional protein structure of fertile and sterile plant Capana05g000747 was predicted. The results showed that the three-dimensional structure of the two proteins differed significantly. Sequence alignment analysis showed that the gene Capana05g000747 has a similar function to gene At2g02148. The gene At2g02148 contains a pentatricopeptide repeat protein which has important physiological functions in the gene expression process of organelles and is closely related to the performance of malesterility genes. Therefore, Capana05g000747 was selected as an important candidate gene for sweet pepper nuclear male sterile testing material AB91. 展开更多
关键词 Sweetpepper Nuclear Malesterile Line Genome-Wide Resequencing Technique Candidate Gene
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