Objective To investigate perfect therapeutic measure of Chinese traditional herbs through observing curative effect of single dose traditional Chinese medicine on murine viral myocarditis(VMC).Method Balb/C mice were ...Objective To investigate perfect therapeutic measure of Chinese traditional herbs through observing curative effect of single dose traditional Chinese medicine on murine viral myocarditis(VMC).Method Balb/C mice were inoculated intraperitoneally with diluted solution of coxsackievirus B3(CVB3) , and then were divided into three groups randomly: infected control group, Mongolian milkvetch root treated group; Indigowood treated group. The mice were put to death and their hearts were examined on day 3,5,7,10,14,21 post inoculated, and were stained with hemotoxylin and eosin(HE) to observe myocardial lesions and to estimate myocardial histopathological scores, simultaneously to separate virus from myocardium. In addition, we observed myocardial ultrastructure by electron microscopy on 7th day post inoculated. Result Compared with infected control group, myocardial histopathological scores of both Mongolian milkvetch root treated group from 5th day to 21st day post inoculated and Indigowood treated group on 5th day and 7th day post inoculated showed significant decrease(P< 0.05).Conclusion Mongolian milkvetch root could protect myocardial cells.展开更多
Salinity stress induces oxidative stress caused by reactive oxygen species(ROS):superoxide radicals,hydrogen peroxide(H2O2) and hydroxyl radicals.Activities of both enzymatic and non-enzymatic components of the antiox...Salinity stress induces oxidative stress caused by reactive oxygen species(ROS):superoxide radicals,hydrogen peroxide(H2O2) and hydroxyl radicals.Activities of both enzymatic and non-enzymatic components of the antioxidant system and related growth parameters were studied in the roots of the salt tolerant rice variety FL478 and the sensitive variety IR29 in response to long term stress(12 d) induced by 50 mmol/L NaCl.The comparative study showed that FL478 maintained higher relative growth rate and lower Na+/K+ in the roots than IR29 due to a higher membrane stability index that effectively exclude Na+.Lower TBARS(thiobarbituric acid reactive substance) content in FL478 roots indicated that its membrane was relatively unaffected by ROS despite high H2O2 content recorded under the salinity stress.Relatively higher superoxide dismutase activity along with a parallel increase in transcript level of superoxide dismutase(Os07g46990) in FL478 indicated that this protein might make a vital contribution to salt stress tolerance.Although the content of ascorbic acid remained unchanged in FL478,the activity of ascorbic peroxidases(APOXs) was reduced comparably in the both varieties.Transcriptomic data showed that a larger number of peroxidase genes were upregulated in FL478 compared to IR29 and several of which might provide engineering targets to improve rice salt tolerance.展开更多
The effect of lanthanum ion on membrane permeability of corn root segment is studied.Results shows thattreatment of the root segments with LaCl<sub>3</sub>·7H<sub>2</sub>O can decrease the...The effect of lanthanum ion on membrane permeability of corn root segment is studied.Results shows thattreatment of the root segments with LaCl<sub>3</sub>·7H<sub>2</sub>O can decrease the ion leakage of the root tissue.The leakagedecreases hy increasing the concentration of lanthanum,and the concentration has no further dependence on ionleakages above 25 μmol/L.After treating the root tissue for one hour with lanthanum ion,the amount of leak-age has linear dependence on time within the first 2 h and after two hours the leakage increases.The ion leakageof the tissue dependes on the time of lanthanum treatment.When the duration of treatment is prolonged,theleakage decreases rapidly and the leakage reaches a minimum at 30 minutes.Other rare earth elements can alsodecrease ion leakage.There is no significant difference in the ion leakage among these elements.展开更多
Effects of calcium on ATPase activities, lipid contents, and fatty acid compositions of plasma membrane from wheat roots were assayed under aluminum stress. The results showed that the increase of calcium concentratio...Effects of calcium on ATPase activities, lipid contents, and fatty acid compositions of plasma membrane from wheat roots were assayed under aluminum stress. The results showed that the increase of calcium concentration in the nutrient solution increased the activity of H + ATPase and the phospholipid content, decreased the activity of Ca 2+ ATPase and the galactolipid of plasma membrane. Owing to the decrease of linolenic acid content, the index of unsaturated fatty acid (IUFA) and index of double bond (DBI) decreased in Altas66. The IUFA and DBI of plasma membrane from Scout66 roots increased because its linolenic acid content increased obviously and its palmitic acid content decreased apparently.展开更多
文摘Objective To investigate perfect therapeutic measure of Chinese traditional herbs through observing curative effect of single dose traditional Chinese medicine on murine viral myocarditis(VMC).Method Balb/C mice were inoculated intraperitoneally with diluted solution of coxsackievirus B3(CVB3) , and then were divided into three groups randomly: infected control group, Mongolian milkvetch root treated group; Indigowood treated group. The mice were put to death and their hearts were examined on day 3,5,7,10,14,21 post inoculated, and were stained with hemotoxylin and eosin(HE) to observe myocardial lesions and to estimate myocardial histopathological scores, simultaneously to separate virus from myocardium. In addition, we observed myocardial ultrastructure by electron microscopy on 7th day post inoculated. Result Compared with infected control group, myocardial histopathological scores of both Mongolian milkvetch root treated group from 5th day to 21st day post inoculated and Indigowood treated group on 5th day and 7th day post inoculated showed significant decrease(P< 0.05).Conclusion Mongolian milkvetch root could protect myocardial cells.
基金funded by Asian Development Bank’s-Distance Education Modernization Programme at the Open University of Sri Lanka to Prasad SENADHEERA
文摘Salinity stress induces oxidative stress caused by reactive oxygen species(ROS):superoxide radicals,hydrogen peroxide(H2O2) and hydroxyl radicals.Activities of both enzymatic and non-enzymatic components of the antioxidant system and related growth parameters were studied in the roots of the salt tolerant rice variety FL478 and the sensitive variety IR29 in response to long term stress(12 d) induced by 50 mmol/L NaCl.The comparative study showed that FL478 maintained higher relative growth rate and lower Na+/K+ in the roots than IR29 due to a higher membrane stability index that effectively exclude Na+.Lower TBARS(thiobarbituric acid reactive substance) content in FL478 roots indicated that its membrane was relatively unaffected by ROS despite high H2O2 content recorded under the salinity stress.Relatively higher superoxide dismutase activity along with a parallel increase in transcript level of superoxide dismutase(Os07g46990) in FL478 indicated that this protein might make a vital contribution to salt stress tolerance.Although the content of ascorbic acid remained unchanged in FL478,the activity of ascorbic peroxidases(APOXs) was reduced comparably in the both varieties.Transcriptomic data showed that a larger number of peroxidase genes were upregulated in FL478 compared to IR29 and several of which might provide engineering targets to improve rice salt tolerance.
文摘The effect of lanthanum ion on membrane permeability of corn root segment is studied.Results shows thattreatment of the root segments with LaCl<sub>3</sub>·7H<sub>2</sub>O can decrease the ion leakage of the root tissue.The leakagedecreases hy increasing the concentration of lanthanum,and the concentration has no further dependence on ionleakages above 25 μmol/L.After treating the root tissue for one hour with lanthanum ion,the amount of leak-age has linear dependence on time within the first 2 h and after two hours the leakage increases.The ion leakageof the tissue dependes on the time of lanthanum treatment.When the duration of treatment is prolonged,theleakage decreases rapidly and the leakage reaches a minimum at 30 minutes.Other rare earth elements can alsodecrease ion leakage.There is no significant difference in the ion leakage among these elements.
文摘Effects of calcium on ATPase activities, lipid contents, and fatty acid compositions of plasma membrane from wheat roots were assayed under aluminum stress. The results showed that the increase of calcium concentration in the nutrient solution increased the activity of H + ATPase and the phospholipid content, decreased the activity of Ca 2+ ATPase and the galactolipid of plasma membrane. Owing to the decrease of linolenic acid content, the index of unsaturated fatty acid (IUFA) and index of double bond (DBI) decreased in Altas66. The IUFA and DBI of plasma membrane from Scout66 roots increased because its linolenic acid content increased obviously and its palmitic acid content decreased apparently.
