Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment...Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment and response surface design was used to optimize the extraction process of polysaccharides.The optimal extraction conditions of polysaccharide were as follows:extraction time 2 h,extraction temperature 40.5℃,solid-liquid ratio 1:15(w/v),and the amount of double enzyme(cellulase:β-glucanase,mass ratio 1:2)2.0%.Under these conditions,the extraction yield of polysaccharide reached(14.86±0.12)%.展开更多
Based on the meteorological data and DEM data in the producing areas of Morchella esculenta in the western Sichuan plateau from 1991 to 2020, the biological characteristics of M. esculenta, as well as the survey of pr...Based on the meteorological data and DEM data in the producing areas of Morchella esculenta in the western Sichuan plateau from 1991 to 2020, the biological characteristics of M. esculenta, as well as the survey of production in the planting area, the correlation between M. esculenta production and the climatic ecological conditions at an altitude of 1 200-3 000 m in the western Sichuan plateau was comprehensively analyzed by using the inverse distance weight method, analytic hierarchy process, climate risk assessment model and geographic information system(GIS), and restrictive or high impact climatic ecological factors were selected as the suitability zoning indicators to carry out the analysis of climatic ecological suitability and planting zoning. The results show that the climatic factors affecting M. esculenta cultivation in the western Sichuan plateau were mainly temperature, temperature difference between day and night, and humidity, and the main meteorological disaster was freezing disaster. Under the influence of vertical changes in temperature, topography and cold damage, the growing areas of M. esculenta in the western Sichuan plateau were mainly distributed in the Minjiang River basin and the river valley along the Dadu River basin at an altitude of 1 200-2 000 m, and were distributed in strips and branches along the rivers. The suitable areas were mainly distributed in Wenchuan, Lixian, Maoxian, Kangding, Jiulong and Luding counties(cities), which were the main producing areas of M. esculenta, but the area was small, accounting for only 3.5% of the study area;the sub-suitable areas were mainly distributed in some towns of Danba, Xiaojin, Wenchuan, Lixian, Maoxian, Heishui and Jiuzhaigou counties, accounting for 36.0% of the total area, and they were the main planting areas of M. esculenta.展开更多
The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica...The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica exhibited higher aver- age activities of protease, catalase and xylanase but lower laccase activity than those of sclerotia-forming strains. However, the differences did not reach significance level. The soluble amylases activity of M. crasspies was significantly higher than that of M. conica, while the mean activity of other enzymes had differences between them, but the differences did not reach significance level.展开更多
Morchella angusticeps Peck,one of the most popular edible mushrooms,has attracted great attention due to its delicious taste and healthy properties.However,both its biological effects and the possible mechanism of act...Morchella angusticeps Peck,one of the most popular edible mushrooms,has attracted great attention due to its delicious taste and healthy properties.However,both its biological effects and the possible mechanism of action have not yet been known.We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck(MPE)against HepG2 human hepatocellular carcinoma cells.Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90%(P<0.01).The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax,Bcl-2 and cleaved caspase-3.On the other hand,MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21,Cyclin D1,cyclin-dependent kinases-4(CDK4)and proliferating cell nuclear antigen(PCNA).Additionally,MPE downregulated TRAF-2 and p-p53,while upregulated p-ASK1 and p-p38.Therefore,it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway.展开更多
Aiming at the climatic characteristics of more rain and high humidity and cultivation habits of rapeseed in Tongren area,in terms of rapeseed and Morchella spp.interplanting,excellent varieties suitable for the local ...Aiming at the climatic characteristics of more rain and high humidity and cultivation habits of rapeseed in Tongren area,in terms of rapeseed and Morchella spp.interplanting,excellent varieties suitable for the local climate were selected,the formulas of culture substrate and nutrition bag were optimized to produce high-quality strains,soil pests were prevented and controlled,rapeseed was transplanted in time,Morchella spp.were sown in ditches,nutrition bags were placed simultaneously,thick soil was covered and fruiting ditches were left,and stress was arranged to promote growth of mycelia.The production cycle was 90-100 d.The yield of Morchella spp.reached 2250-3375 kg/ha,with a net profit of 6000-10000 yuan.展开更多
The pollution and safety of Pb,Cd,Cr,Hg,and As in soils of six habitats of Morchella in Guizhou Province were evaluated based on single factor pollution index(PI),Nemerow’s pollution index(NPI),and assessment of heal...The pollution and safety of Pb,Cd,Cr,Hg,and As in soils of six habitats of Morchella in Guizhou Province were evaluated based on single factor pollution index(PI),Nemerow’s pollution index(NPI),and assessment of health risk from exposure to the soils.As indicated by PI,the bases,except for base F,all suffered from the pollution by the five heavy metals,particularly Cd.NPI suggested warningline pollution in bases C and D,slight pollution in bases A and E,and no pollution in bases B and F.As for the health risk from exposure to the soil,the ADD was in the order of ADD_(ingestion)>ADD_(dermal contact)>ADD_(inhalation) and HQ followed the order of HQ_(ingestion)>HQ_(dermal contact)>HQ_(inhalation).However,the HQ values were<1,indicating no cancer risk in the study areas.展开更多
基金Supported by The Key Research and Development Program of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization(202141204)The 2024 Huanggang Municipal Level Science and Technology Innovation Special Fund(YBXM20240031).
文摘Morchella septimelata polysaccharide(MSP)possesses multiple biological activities.In this study,double enzyme method was used to extract polysaccharides from Morchella septimelata.The basis of single factor experiment and response surface design was used to optimize the extraction process of polysaccharides.The optimal extraction conditions of polysaccharide were as follows:extraction time 2 h,extraction temperature 40.5℃,solid-liquid ratio 1:15(w/v),and the amount of double enzyme(cellulase:β-glucanase,mass ratio 1:2)2.0%.Under these conditions,the extraction yield of polysaccharide reached(14.86±0.12)%.
基金Supported by the Second Tibetan Plateau Scientific Expedition and Research Pro-gram(STEP)(2019QZKK0303-02)the Foundation of Science and Technology Development of Sichuan Province Key Laboratory of Heavy Rain and Drought-Flood Disasters in Plateau and Basin(SCQXKJYJXMS202109).
文摘Based on the meteorological data and DEM data in the producing areas of Morchella esculenta in the western Sichuan plateau from 1991 to 2020, the biological characteristics of M. esculenta, as well as the survey of production in the planting area, the correlation between M. esculenta production and the climatic ecological conditions at an altitude of 1 200-3 000 m in the western Sichuan plateau was comprehensively analyzed by using the inverse distance weight method, analytic hierarchy process, climate risk assessment model and geographic information system(GIS), and restrictive or high impact climatic ecological factors were selected as the suitability zoning indicators to carry out the analysis of climatic ecological suitability and planting zoning. The results show that the climatic factors affecting M. esculenta cultivation in the western Sichuan plateau were mainly temperature, temperature difference between day and night, and humidity, and the main meteorological disaster was freezing disaster. Under the influence of vertical changes in temperature, topography and cold damage, the growing areas of M. esculenta in the western Sichuan plateau were mainly distributed in the Minjiang River basin and the river valley along the Dadu River basin at an altitude of 1 200-2 000 m, and were distributed in strips and branches along the rivers. The suitable areas were mainly distributed in Wenchuan, Lixian, Maoxian, Kangding, Jiulong and Luding counties(cities), which were the main producing areas of M. esculenta, but the area was small, accounting for only 3.5% of the study area;the sub-suitable areas were mainly distributed in some towns of Danba, Xiaojin, Wenchuan, Lixian, Maoxian, Heishui and Jiuzhaigou counties, accounting for 36.0% of the total area, and they were the main planting areas of M. esculenta.
基金Supported by China Agriculture Research System(CARS24)National High Technology Research and Development Program of China(863 Program,2007AA021506)~~
文摘The extracellular enzyme activities of sclerotia-forming and nonsclerotia- forming single spore strains of Morchella conica and M. crasspies were determined and compared. The non sclerotia-forming strains of M. conica exhibited higher aver- age activities of protease, catalase and xylanase but lower laccase activity than those of sclerotia-forming strains. However, the differences did not reach significance level. The soluble amylases activity of M. crasspies was significantly higher than that of M. conica, while the mean activity of other enzymes had differences between them, but the differences did not reach significance level.
基金This research was supported by the National Natural Science Foundation of China(31471576,31271825)the Fundamental Research Funds for the Central Universities,China(XDJK2018B030,SWU118012).
文摘Morchella angusticeps Peck,one of the most popular edible mushrooms,has attracted great attention due to its delicious taste and healthy properties.However,both its biological effects and the possible mechanism of action have not yet been known.We investigated the anti-proliferative activity of the phenolic extract derived from Morchella angusticeps Peck(MPE)against HepG2 human hepatocellular carcinoma cells.Results showed that MPE at non-cytotoxicity doses significantly inhibited the proliferation of HepG2 cells in a dose-dependent manner with inhibitory rates ranging from 18 to 90%(P<0.01).The possible mechanism might be that MPE induced apoptosis through initiating the mitochondrial death pathway by regulating Bax,Bcl-2 and cleaved caspase-3.On the other hand,MPE might trigger cell cycle arrest at G0/G1/S phases by managing p21,Cyclin D1,cyclin-dependent kinases-4(CDK4)and proliferating cell nuclear antigen(PCNA).Additionally,MPE downregulated TRAF-2 and p-p53,while upregulated p-ASK1 and p-p38.Therefore,it could be inferred that MPE might induce the anti-proliferative function to HepG2 cells through the p38/MAPK signal transduction pathway.
基金Supported by Excellent Youth Talents Project of Guizhou Province Department of Forestry(Qian Lin Ke He J Zi[2021]09)Science and Technology Plan Project of Tongren City([2019]116,[2020]46,[2020]49).
文摘Aiming at the climatic characteristics of more rain and high humidity and cultivation habits of rapeseed in Tongren area,in terms of rapeseed and Morchella spp.interplanting,excellent varieties suitable for the local climate were selected,the formulas of culture substrate and nutrition bag were optimized to produce high-quality strains,soil pests were prevented and controlled,rapeseed was transplanted in time,Morchella spp.were sown in ditches,nutrition bags were placed simultaneously,thick soil was covered and fruiting ditches were left,and stress was arranged to promote growth of mycelia.The production cycle was 90-100 d.The yield of Morchella spp.reached 2250-3375 kg/ha,with a net profit of 6000-10000 yuan.
文摘The pollution and safety of Pb,Cd,Cr,Hg,and As in soils of six habitats of Morchella in Guizhou Province were evaluated based on single factor pollution index(PI),Nemerow’s pollution index(NPI),and assessment of health risk from exposure to the soils.As indicated by PI,the bases,except for base F,all suffered from the pollution by the five heavy metals,particularly Cd.NPI suggested warningline pollution in bases C and D,slight pollution in bases A and E,and no pollution in bases B and F.As for the health risk from exposure to the soil,the ADD was in the order of ADD_(ingestion)>ADD_(dermal contact)>ADD_(inhalation) and HQ followed the order of HQ_(ingestion)>HQ_(dermal contact)>HQ_(inhalation).However,the HQ values were<1,indicating no cancer risk in the study areas.
