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Development of Isogenic Lines of Morphological Markers in Indica Rice 被引量:5
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作者 曾大力 钱前 +7 位作者 董国军 朱旭东 董凤高 滕胜 郭龙标 曹立勇 程式华 熊振民 《Acta Botanica Sinica》 CSCD 2003年第9期1116-1120,共5页
A total of 28 accessions of rice (Oryza sativa L.) germplasms were collected from International Rice Research Institute (IRRI), Philippine and Gene Pool of China National Rice Research Institute (CNRRI). These germpla... A total of 28 accessions of rice (Oryza sativa L.) germplasms were collected from International Rice Research Institute (IRRI), Philippine and Gene Pool of China National Rice Research Institute (CNRRI). These germplasms were attributed as a Donor Gene Pool, consisting of 27 diverse morphological marker lines with each having a unique valuable trait (gene), and the recurrent parent-Zhefu 802, an early-season indica cultivar in south of China in the 1980s and 1990s. A set of 27 isogenic lines with 2-3 markers on every chromosome were obtained after more than 10 successive backcrosses. Moreover, 15 accessions of double-marker isogenic lines were developed by further pyramiding markers on the same chromosome. Key agronomic traits such as heading date, plant height, tillering number and panicle length in this set of lines were similar to that of Zhefu 802 except the marker traits. 展开更多
关键词 indica rice agronomic trait morphological marker isogenic lines
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Charaterization of Citrus Hybrid "Huangguogan" through the Combination of Morphological and Molecular Markers 被引量:3
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作者 Xue-Fei Wang Xi-Rui Xiong Xue-Li Pu Qiao-Qiao Yan Bo Xiong Feng-Ling Liao Qian-Qian Fan Zhi-Hui Wang 《Journal of Life Sciences》 2013年第4期348-352,共5页
Huangguogan, an obvious Citrus hybrid, is suitable for transportation and export and ripens in March or April. Because of late season, it may playa significant role in fruit market. However, its origin is still unconf... Huangguogan, an obvious Citrus hybrid, is suitable for transportation and export and ripens in March or April. Because of late season, it may playa significant role in fruit market. However, its origin is still unconfirmed. The aim of this study was to clarify the possible parentage of Huangguogan via the combination of morphological and molecular markers including simple sequence repeat (SSR) and chloroplast simple sequence repeat (cpSSR). Analysis of morphological traits including leaf stalk length, phylliform index and fruit shape index indicated that Huangguogan had similarities in morphology with Sweet orange. The SSR Cluster Analysis showed that Huangguogan was clustered together with Hongju tangerine and revealed -80% genetic similarity. They illustrated a close genetic distance between Huangguogan and Hongju tangerine. In addition, the bands of2 polymorphic cpSSR were identical in Huangguoggan and Sweet orange. Consequently, it is likely that its female parentage was the sweet orange (Citrus sinensis (L.) Osbeck) and its male parentage was the tangerine (Citrus reticulata Blanco). 展开更多
关键词 CITRUS natural hybrid huangguogan morphological and molecular markers.
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Genetic Diversity in the Semi-Arid Grown Cowpea(<i>Vigna unguiculata</i>L. Walp)Accessions Using Morphological and Molecular Characterization
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作者 Ahmed A. Elteib Seif M. Gasim 《Agricultural Sciences》 2020年第8期692-706,共15页
Assessment of genetic diversity of the indigenous crop accessions is extremely important for breeders to identify potential parents in cross-breeding programs. Fourteen cowpea accessions collected from different part&... Assessment of genetic diversity of the indigenous crop accessions is extremely important for breeders to identify potential parents in cross-breeding programs. Fourteen cowpea accessions collected from different part<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> of Sudan were used for characterization at morphological and molecular levels. The seeds of the accessions were sown in the field using a randomized complete block design with three replicates. Sixteen morphological descriptors (9 qualitative and 7 quantitative) and 20 Random Amplified Polymorphic DNA (RAPD) markers were used for characterization of the accessions. The results of morphological data revealed considerable variability within and between state’s accessions. Some morphological traits revealed similarity between accessions from different states. Among the 20 RAPD markers used, 18 were polymorphic. A total of 379 polymorphic patterns were generated;polymorphic information content (PIC) ranged from 0.63 to 0.98 with an average of 0.9. The number of fragment detected ranged from 2 for OPL-11 to 51 for OPY-2 with an average of 26.06/primer and 27.07/genotype. One to five (1</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">-</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">5) unique fragments of different sizes were detected for particular accessions, which may provide a valuable resource for breeding superior cowpea cultivars in Sudan and other semi-arid zones. Genetic similarity was ranged from 0.02 to 0.47 with an average of 0.25. Highest genetic similarity was between genotypes HSD-2966 and HSD-2967 and between genotypes HSD-5131</span></span></span><span><span><span style="font-family:;" "=""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">and HSD-5627 and the lowest was between HSD-5131 and HSD-5861 followed by that between HSD-2976 and HSD-29130 accessions. The study recommend</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> the combination of morphological and molecular data for more efficient genetic diversity assessment and management.</span></span></span> 展开更多
关键词 Cowpea Accessions Genetic Diversity Characterization morphological markers RADP markers Polymorphism
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Morphological and Genetical Variability among Rhizoctonia solani Isolates Causing Sheath Blight Disease of Rice 被引量:2
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作者 Zakiah Rahman MONI Md Ansar ALI +6 位作者 Md Shahidul ALAM Md Asif RAHMAN Md Rejwan BHUIYAN Md Salim MIAN Khandakar Md IFTEKHARUDDAULA Md Abdul LATIF Mohammad Ashik Iqbal KHAN 《Rice science》 SCIE CSCD 2016年第1期42-50,共9页
Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a refe... Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a reference isolate confirmed that all the isolates belonged to R. solani. Significant variation was observed in sclerotial size, shape and distribution. Un-weighted pair group method with arithmetic mean dendrogram constructed based on the Gower's general similarity coefficient showed that these isolates were grouped into four clusters at the 0.68 similarity coefficent according to morphological characters. Cluster I was a major cluster consisting of 13 isolates, while clusters Ⅱ to Ⅳ consisted of 1 or 2 isolates. Analyses by variable number of tandem repeat and amplified fragment length polymorphism markers showed that the isolates were grouped into five and three clusters at a similarity coefficient of 0.64 and 0.69, respectively. Although most of the variability was found between isolates from different regions as expected, significant variation was observed within the isolates collected from similar agro-ecological regions. Our results suggest the presence of different races of R. solani within the same local geographic regions. 展开更多
关键词 rice Rhizoctonia solani fungal variability molecular marker morphological character
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Research Progress on Genetic Diversity of Cultivated Soybean Germplasm Resources in China 被引量:1
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作者 Yongchao GONG Dongtao SONG +3 位作者 Nana LI Yanyan PU Tingjing YAN Hanfeng DING 《Agricultural Biotechnology》 CAS 2018年第2期47-52,67,共7页
In this paper,by analyzing the genetic diversity of cultivated soybean germplasm resources in China,the environmental and genotypic factors that affect the genetic diversity of cultivated soybean germplasm resources w... In this paper,by analyzing the genetic diversity of cultivated soybean germplasm resources in China,the environmental and genotypic factors that affect the genetic diversity of cultivated soybean germplasm resources were explored to further expand the genetic basis of the existing germplasm resources of cultivated soybean in China. Moreover,research progress on genetic diversity of cultivated soybean in China was summarized,which not only revealed the geographical characteristics of genetic diversity of cultivated soybean in China,but also proposed direction for research of genetic diversity of soybean. 展开更多
关键词 Chinese soybean Germplasm resources Genetic diversity morphological markers Biochemical markers RAPD SSRHome
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Molecular Tagging and Mapping of Quantitative Trait Loci for Lint Percentage and Morphological Marker Genes in Upland Cotton 被引量:15
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作者 Wang-Zhen Guo Guo-Jia Ma Yi-Chao Zhu Chen-Xin Yi Tian-Zhen Zhang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2006年第3期320-326,共7页
Using 219 F2 Individuals developed by crossing the genetic standard line TM-1 and the multiple dominant marker line T586 In Gossyplum hirsutum L., a genetic linkage map with 19 linkage groups was constructed based on ... Using 219 F2 Individuals developed by crossing the genetic standard line TM-1 and the multiple dominant marker line T586 In Gossyplum hirsutum L., a genetic linkage map with 19 linkage groups was constructed based on simple sequence repeat (SSR) markers. Compared with our tetraploid backboned molecular genetic map from a (TM-1xHal 7124)xTM-1 BC1 population, 17 of the 19 I|nkage groups were combined and anchored to 12 chromosomes (sub-genomes). Of these groups, four morphological marker genes In T586 had been mapped Into the molecular linkage map. Meanwhile, three quantitative trait loci for lint percentage were tagged and mapped separately on the A03 linkage group and chromosome 6. 展开更多
关键词 genetic linkage map association morphological markers quantitative trait locus tagging upland cotton.
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