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恶性疟原虫环子孢子蛋白四肽重复序列单克隆抗体的制备 被引量:3
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作者 陆俭 李萍 +3 位作者 马亚茹 李刚 陈勇 蒋琳 《中国生物制品学杂志》 CAS CSCD 2015年第10期1039-1043,共5页
目的制备针对恶性疟原虫环子孢子蛋白(circumsporozoite protein,CSP)NANP四肽重复序列[Asn-Ala-AsnPro(NANP)repeated motif]的单克隆抗体。方法采用马来酰亚胺修饰的BSA与(NANP)5C偶联法及ADH-EDC介导的BSA与(NANP)5偶联法,... 目的制备针对恶性疟原虫环子孢子蛋白(circumsporozoite protein,CSP)NANP四肽重复序列[Asn-Ala-AsnPro(NANP)repeated motif]的单克隆抗体。方法采用马来酰亚胺修饰的BSA与(NANP)5C偶联法及ADH-EDC介导的BSA与(NANP)5偶联法,将合成的NANP多肽与载体蛋白偶联,免疫BALB/c小鼠后,采用免疫后小鼠脾细胞与骨髓瘤细胞SP2/0杂交的细胞融合技术,获得分泌抗(NANP)5多肽单克隆抗体的阳性杂交瘤细胞株,间接ELISA法检测效价。通过免疫F1小鼠诱生腹水,采用50%硫酸铵沉淀法盐析纯化后,进行单克隆抗体亚类和特异性鉴定。结果采用2种方法制备了BSA-(NANP)5偶联蛋白,以该蛋白为抗原,获得10株抗(NANP)5多肽阳性杂交瘤细胞株,效价在1∶80 000~1∶640 000之间,均为Ig G1亚类,轻链类型为κ,可被(NANP)5及CSP抗原特异性识别。结论成功制备了抗(NANP)5多肽的单克隆抗体,该抗体可在恶性疟疾疫苗的研发中发挥重要作用。 展开更多
关键词 疟疾 环子孢子蛋白 NANP 四肽重复序列 单克隆抗体
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Soundscape of the West Lake Scenic Area with profound cultural background—a case study of Evening Bell Ringing in Jingci Temple,China 被引量:9
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作者 Jian GE Min GUO Miao YUE 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2013年第3期219-229,共11页
From the case study of Evening Bell Ringing at Nanping Hill,one of the West Lake Cultural Landscapes in Hangzhou,China,we investigated the soundscape of a scenic area with a profound cultural background.First,we condu... From the case study of Evening Bell Ringing at Nanping Hill,one of the West Lake Cultural Landscapes in Hangzhou,China,we investigated the soundscape of a scenic area with a profound cultural background.First,we conducted the soundscape physical index of the area in both winter and spring seasons to analyze its objective graphical expression.Second,we focused on people's reactions to the soundscape in order to obtain a subjective evaluation of each component in the soundscape and integrated environment.Then,the relationship between the objective data and the subjective evaluation was analyzed.Finally,the impacts of the natural environment,history,and cultural factors on the evaluation of the Jingci Temple soundscape were studied.It was found that natural sounds,cultural sounds,and historic sounds were widely acclaimed in people's subjective feelings,which indicated the close relationships among historical and cultural background,soundscape,and natural environment.Thus,the conclusion was made that soundscape should be consistent with the local natural environment and the historical and cultural background. 展开更多
关键词 SOUNDSCAPE Culture Evaluation West Lake Evening Bell Ringing at nanping Hill
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恶性疟原虫CSP蛋白重复序列对肝素结合的影响 被引量:1
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作者 隋委伽 史小雨 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2018年第3期66-71,共6页
疟疾是1种严重的传染性寄生虫病,其病原体为疟原虫,而子孢子(sporozoite)则是其传染性阶段.主要研究感染人体的恶性疟原虫(Plasmodium falciparum,Pf)的主要子孢子表面蛋白:环子孢子蛋白CSP(circumsporozoite protein).CSP可以识别肝细... 疟疾是1种严重的传染性寄生虫病,其病原体为疟原虫,而子孢子(sporozoite)则是其传染性阶段.主要研究感染人体的恶性疟原虫(Plasmodium falciparum,Pf)的主要子孢子表面蛋白:环子孢子蛋白CSP(circumsporozoite protein).CSP可以识别肝细胞表面的硫酸乙酰软骨素(heparan sulfate proteoglycans,HSPGs),从而介导疟原虫子孢子对肝实质细胞的入侵,特异性识别CSP的抗体也是目前为止颇有希望的防疟手段.CSP由N端结构域,中央NANP重复序列(Central NANP Repeats)和C端的TSR(thrombospodin type-I repeat)结构域组成.前期研究显示,CSP中不同区域位点的缺失或突变对CSP结合肝实质细胞产生不同程度的影响,但中央NANP重复序列在肝素识别中的作用还不清楚.通过构建了缺失全部重复序列的CSP突变体,并进行突变体的纯化和肝素亲和实验,发现重复序列的缺失并不影响CSP蛋白的表达及与肝素的相互作用.这些结果为进一步研究CSP的结构和功能提供了理论依据. 展开更多
关键词 恶性疟原虫 环子孢子蛋白CSP 中央NANP重复序列 肝素
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