Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water ex...Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment. EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails’ soft tissues greatly, and also the protein content. Conclusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason for the molluscicidal activities. The biochemical mechanism needs further research.展开更多
[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. ve...[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. vespertilio, the insecticidal effect of water extract of oleander against the larvae was studied. [Result] The 1/20-1/200 times water extract of dry leaf weight of oleander had better insecticidal effect against the larvae of E. vespertilio, and the feeding mortality rate reached 100%. [Conclusion] The paper provided reference for development of new botanical pesticides with high efficiency, environmental protection and low carbon.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 30471506).
文摘Objective To evaluate the molluscicidal activities of three Chinese plants N. indicum Mill, P. stenoptera DC, and R. japonicum Houtt, and to clarify the molluscicidal mechanism. Methods N-butanol extracts and water extracts of the three plants were obtained. The reactions of EST isozyme, glycogen and total protein of snails to the plant extracts were studied. Results EST electrophoresis showed that EST was an important antidotal enzyme system and reacted strongly to environment. EST changed greatly during the whole exposure period so that it could be viewed as a pathological index of toxicity. Extracts decreased the glycogen content of the snails’ soft tissues greatly, and also the protein content. Conclusion All extracts show strong molluscicidal activity. The LD50 value of the water extract of N. indicum Mill is as low as 13.2 mg/L. EST can be viewed as a pathological index of toxicity. The energy metabolism abnormity is the key reason for the molluscicidal activities. The biochemical mechanism needs further research.
基金Acknowledgments The authors would like to thank the National Natural Science Foundation of China （grant No. 30471506, 30570322, 39670654, 30671818） and the Science and Technology Foundation of Forest （No. 2006BAD03AI 5） for providing funds to the research.
基金Supported by Innovation Experiment Program of Jiangsu Provincial College Students(5731506510)
文摘[Objective] The paper was to study the insecticidal effect of water extract of oleander (Nerium indicum) against the larvae of Enmonodia vespertilio Fabriciu. [Method] Through feeding experiment on the larvae of E. vespertilio, the insecticidal effect of water extract of oleander against the larvae was studied. [Result] The 1/20-1/200 times water extract of dry leaf weight of oleander had better insecticidal effect against the larvae of E. vespertilio, and the feeding mortality rate reached 100%. [Conclusion] The paper provided reference for development of new botanical pesticides with high efficiency, environmental protection and low carbon.
