本文以HeLa细胞为材料研究一种着丝粒蛋白CENP-B的基因表达与细胞周期及细胞核骨架的关系。将HeLa细胞同步在不同周期时相,以流式细胞光度术、同位素掺入和ACA着丝粒染色等方法检测细胞同步化效果。我们分别提取了各周期时相细胞的总RNA...本文以HeLa细胞为材料研究一种着丝粒蛋白CENP-B的基因表达与细胞周期及细胞核骨架的关系。将HeLa细胞同步在不同周期时相,以流式细胞光度术、同位素掺入和ACA着丝粒染色等方法检测细胞同步化效果。我们分别提取了各周期时相细胞的总RNA和Poly(A)^+RNA,用Dot blot和Northern blot杂交方法研究CENP-B在细胞周期中的表达。结果表明,CENP-B基因在细胞周期中的各个时相均有表达,但表达的强度差别很大:G2期表达最强,S期最弱,G1期中的表达介于二者之间;有意义的是CENP-B基因在M期仍然有较强的表达,表现出其在细胞周期中表达的持续性;这种表达的持续性反映了一种可能性:着丝粒、动粒蛋白不断合成,但直到S期后进入G2期时着丝粒、动粒蛋白到一定临界浓度时才开始组装新的动粒。另外,着丝粒、动粒蛋白的持续合成对着丝粒、动粒功能的发挥可能是必需的。用Bam H I限制性内切酶消化处于不同细胞周期时相的HeLa细胞核骨架,提取与核骨架紧密结合的DNA,用^(32)P标记的cDNA为探针研究CENP-B基因与细胞核骨架的结合与其表达的关系。结果证明,在G2期细胞中CENP-B基因表达最强,与细胞核骨架结合最为紧密,G1期细胞中次之,S期中CENP-B基因与核骨架结合最弱,说明CENP-B基因与细胞核骨架结合的紧密度影响其表达强度。展开更多
In this paper, we present a novel method for digital nuclear signal processing based on image processing and recognition, which can improve signal-to-noise ratio of digital nuclear signal effectively without changing ...In this paper, we present a novel method for digital nuclear signal processing based on image processing and recognition, which can improve signal-to-noise ratio of digital nuclear signal effectively without changing the signal shape. The digital nuclear signal with a "time-amplitude" series is converted into a grayscale image with adjustable pixel size. Template of the converted image is extracted by means of modern image processing methods, such as spatial digital low-pass filtering, image binary and the skeleton extracting of images. The needed parameters are extracted from the template image. The method of template extracting presented in this paper can be used flexibly to extract template of nuclear signals, whether the whole or even part of that, and got multi templates corresponding to the whole or partial characters of the signals. The results of image processing,along with γ-ray energy spectrum of241 Am acquired by this method, show that the new method provides a way to develop future digital nuclear instruments of high efficiency and flexibility, high density and multi parameters.展开更多
With gentle cell extraction techniques, various DNA components in the HeLa cells after 6 h of adenovirus infection have been obtained. Adenovirus, early transcribed regions (El_a El_b) and a late transcribed region (L...With gentle cell extraction techniques, various DNA components in the HeLa cells after 6 h of adenovirus infection have been obtained. Adenovirus, early transcribed regions (El_a El_b) and a late transcribed region (L_2) were used as probes in Southern hybridization, respectively. The experiment showed that only actively transcribed adenovirus DNA fragments would tightly bind to the nuclear matrix of host cells. We inferred that the nuclear matrix of host cells plays an important role in viral DNA transcription.展开更多
文摘本文以HeLa细胞为材料研究一种着丝粒蛋白CENP-B的基因表达与细胞周期及细胞核骨架的关系。将HeLa细胞同步在不同周期时相,以流式细胞光度术、同位素掺入和ACA着丝粒染色等方法检测细胞同步化效果。我们分别提取了各周期时相细胞的总RNA和Poly(A)^+RNA,用Dot blot和Northern blot杂交方法研究CENP-B在细胞周期中的表达。结果表明,CENP-B基因在细胞周期中的各个时相均有表达,但表达的强度差别很大:G2期表达最强,S期最弱,G1期中的表达介于二者之间;有意义的是CENP-B基因在M期仍然有较强的表达,表现出其在细胞周期中表达的持续性;这种表达的持续性反映了一种可能性:着丝粒、动粒蛋白不断合成,但直到S期后进入G2期时着丝粒、动粒蛋白到一定临界浓度时才开始组装新的动粒。另外,着丝粒、动粒蛋白的持续合成对着丝粒、动粒功能的发挥可能是必需的。用Bam H I限制性内切酶消化处于不同细胞周期时相的HeLa细胞核骨架,提取与核骨架紧密结合的DNA,用^(32)P标记的cDNA为探针研究CENP-B基因与细胞核骨架的结合与其表达的关系。结果证明,在G2期细胞中CENP-B基因表达最强,与细胞核骨架结合最为紧密,G1期细胞中次之,S期中CENP-B基因与核骨架结合最弱,说明CENP-B基因与细胞核骨架结合的紧密度影响其表达强度。
基金Supported by National Natural Science Foundation of China(No.11075111)
文摘In this paper, we present a novel method for digital nuclear signal processing based on image processing and recognition, which can improve signal-to-noise ratio of digital nuclear signal effectively without changing the signal shape. The digital nuclear signal with a "time-amplitude" series is converted into a grayscale image with adjustable pixel size. Template of the converted image is extracted by means of modern image processing methods, such as spatial digital low-pass filtering, image binary and the skeleton extracting of images. The needed parameters are extracted from the template image. The method of template extracting presented in this paper can be used flexibly to extract template of nuclear signals, whether the whole or even part of that, and got multi templates corresponding to the whole or partial characters of the signals. The results of image processing,along with γ-ray energy spectrum of241 Am acquired by this method, show that the new method provides a way to develop future digital nuclear instruments of high efficiency and flexibility, high density and multi parameters.
基金Project supported by the National Natural Science Foundation of China
文摘With gentle cell extraction techniques, various DNA components in the HeLa cells after 6 h of adenovirus infection have been obtained. Adenovirus, early transcribed regions (El_a El_b) and a late transcribed region (L_2) were used as probes in Southern hybridization, respectively. The experiment showed that only actively transcribed adenovirus DNA fragments would tightly bind to the nuclear matrix of host cells. We inferred that the nuclear matrix of host cells plays an important role in viral DNA transcription.
