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Genome-wide identification and expression profiling of photosystem II(PsbX)gene family in upland cotton(Gossypium hirsutum L.)
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作者 RAZA Irum PARVEEN Abida +4 位作者 AHMAD Adeel HU Daowu PAN Zhaoe ALI Imran DU Xiongming 《Journal of Cotton Research》 CAS 2024年第1期1-14,共14页
Background Photosystem II(PSII)constitutes an intricate assembly of protein pigments,featuring extrinsic and intrinsic polypeptides within the photosynthetic membrane.The low-molecular-weight transmembrane protein Psb... Background Photosystem II(PSII)constitutes an intricate assembly of protein pigments,featuring extrinsic and intrinsic polypeptides within the photosynthetic membrane.The low-molecular-weight transmembrane protein PsbX has been identified in PSII,which is associated with the oxygen-evolving complex.The expression of PsbX gene protein is regulated by light.PsbX’s central role involves the regulation of PSII,facilitating the binding of quinone molecules to the Qb(PsbA)site,and it additionally plays a crucial role in optimizing the efficiency of photosynthesis.Despite these insights,a comprehensive understanding of the PsbX gene’s functions has remained elusive.Results In this study,we identified ten PsbX genes in Gossypium hirsutum L.The phylogenetic analysis results showed that 40 genes from nine species were classified into one clade.The resulting sequence logos exhibited substantial conservation across the N and C terminals at multiple sites among all Gossypium species.Furthermore,the ortholo-gous/paralogous,Ka/Ks ratio revealed that cotton PsbX genes subjected to positive as well as purifying selection pressure might lead to limited divergence,which resulted in the whole genome and segmental duplication.The expression patterns of GhPsbX genes exhibited variations across specific tissues,as indicated by the analysis.Moreover,the expression of GhPsbX genes could potentially be regulated in response to salt,intense light,and drought stresses.Therefore,GhPsbX genes may play a significant role in the modulation of photosynthesis under adverse abiotic conditions.Conclusion We examined the structure and function of PsbX gene family very first by using comparative genom-ics and systems biology approaches in cotton.It seems that PsbX gene family plays a vital role during the growth and development of cotton under stress conditions.Collectively,the results of this study provide basic information to unveil the molecular and physiological function of PsbX genes of cotton plants. 展开更多
关键词 photosystem PHYLOGENETIC SYNTENY RNA seq Gene expression Orthologous
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Effects of drought treatment on photosystemⅡactivity in the ephemeral plant Erodium oxyrhinchum
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作者 CHEN Yingying LIN Yajun +3 位作者 ZHOU Xiaobing ZHANG Jing YANG Chunhong ZHANG Yuanming 《Journal of Arid Land》 SCIE CSCD 2023年第6期724-739,共16页
Drought is a critical limiting factor affecting the growth and development of plants in arid and semi-arid areas.Photosynthesis,one of the most important physiological processes of plants,can be significantly inhibite... Drought is a critical limiting factor affecting the growth and development of plants in arid and semi-arid areas.Photosynthesis,one of the most important physiological processes of plants,can be significantly inhibited by drought.PhotosystemⅡ(PSⅡ)is considered the main attack target when photosynthesis is affected by drought.To clarify how PSⅡcomponents of the ephemeral plant Erodium oxyrhinchum(grown in the Gurbantunggut Desert,China)respond to drought treatment,we evaluated the functional activity of PSII by determining chlorophyll fluorescence and gas exchange parameters under different drought treatment levels(control(400 mL),moderate drought(200 mL),and severe drought(100 m L)).Under moderate drought treatment,significant decreases were found in net photosynthetic rate(Pn),effective quantum yield of PSII(Y(Ⅱ)),relative electron transfer rate of PSII(rETR(Ⅱ)),oxygen-releasing complex,probability of an absorbed exciton moving an electron into the electron transport chain beyond primary quinone receptor Q_(A)-(Φ(E_(o))),probability of a trapped exciton moving an electron into the electron transport chain beyond primary quinone receptor Q_(A)-(ψ(E_(o))),and performance index of PSⅡ(PI_(abs)).Compared to control treatment,marked increases were observed in water use efficiency(WUE),relative variable fluorescence at the J step(V_(J)),initial fluorescence(F_(o)),and dissipated energy per active reaction center(DI_(o)/RC)under moderate drought treatment,but there were no substantial changes in semi-saturated light intensity(I_(K)),active reaction centers per cross-section(RC/CS),and total performance index of PSII and PSI(PI_(total),where PSI is the photosystemⅠ).The changes of the above parameters under severe drought treatment were more significant than those under moderate drought treatment.In addition,severe drought treatment significantly increased the absorbed energy per active reaction center(ABS/RC)and trapping energy per active reaction center(TR_(o)/RC)but decreased the energy transmission connectivity of PSⅡcomponents,RC/CS,and PI_(total),compared to moderate drought and control treatments.Principle component analysis(PCA)revealed similar information according to the grouping of parameters.Moderate drought treatment was obviously characterized by RC/CS parameter,and the values of F_(o),V_(J),ABS/RC,DI_(o)/RC,and TR_(o)/RC showed specific reactions to severe drought treatment.These results demonstrated that moderate drought treatment reduced the photochemical activity of PSII to a certain extent but E.oxyrhinchum still showed strong adaptation against drought treatment,while severe drought treatment seriously damaged the structure of PSⅡ.The results of this study are useful for further understanding the adaptations of ephemeral plants to different water conditions and can provide a reference for the selection of relevant parameters for photosynthesis measurements of large samples in the field. 展开更多
关键词 chlorophyll fluorescence drought treatment electron transport PHOTOSYNTHESIS photosystem II Erodium oxyrhinchum Gurbantunggut Desert
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Effect of high light and desiccation on photosystem Ⅱ in the seedlings and mature plants of tropical seagrass Enhalus acoroides during low tide
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作者 Xingkai CHE Hu LI +1 位作者 Litao ZHANG Jianguo LIU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第1期241-250,共10页
During low tide,the intertidal seagrass Enhalus acoroides is often exposed to high light and desiccation,which can seriously threaten its survival,at least partly by inhibiting photosystem Ⅱ(PSⅡ)activity.The respons... During low tide,the intertidal seagrass Enhalus acoroides is often exposed to high light and desiccation,which can seriously threaten its survival,at least partly by inhibiting photosystem Ⅱ(PSⅡ)activity.The response of leaves of E.acoroides to high light and desiccation was compared for seedlings and mature plants.Results show that the resistance of seedling and mature leaves to high light was quite similar,but to desiccation was very different.Seedling leaves were more sensitive to desiccation than the mature plant leaves,but had better water retention.The damage of desiccation to seedling leaves was mainly caused by dehydration,whereas that to mature plant leaves was caused by hypersaline toxicity.The recovery rate of PSⅡ of seedling leaves was significantly slower than that of the mature plants after the stresses disappeared,which may at least partly contribute to seedling mortality in the wild.In addition,compared to high light,desiccation seriously inhibited the recovery rate of PSⅡ activities even if the leaves became fully rehydrated to their normal relative water content(RWC)in the following re-immersion.Desiccation inhibited the recovery rate of RC/CS_(M)(reaction center per cross section(at t=t_(Fm)))to decrease the production of assimilatory power,which maybe the cause of the slower PSⅡ recovery in desiccation treatments.This study demonstrates that desiccation particularly coupling with high light have a very negative ef fect on the PSⅡ of E.acoroides during low tide and the sensitivity of seedlings and mature plants to desiccation is significantly different,which have important reference significance to choose an appropriate transplanting depth where seedlings and mature plants of E.acoroides not only receive sufficient light for growth,but also that minimize desiccation stress during low tide. 展开更多
关键词 Enhalus acoroides high light DESICCATION photosystem
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Photosystem Development in Dark-grown Lotus (Nelumbo nucifera) Seedlings 被引量:5
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作者 季宏伟 唐崇钦 +1 位作者 李良璧 匡廷云 《Acta Botanica Sinica》 CSCD 2001年第11期1129-1133,共5页
It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grow... It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active. 展开更多
关键词 LOTUS photosystem development dark-grown Western blots
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Orientation of Pigments in the Isolated PhotosystemⅡSub-core Reaction Center CP47/D1/D2/Cyt b-559 Complexes:A Linear Dichroism Study 被引量:3
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作者 侯建敏 Dominique DEJONGHE +2 位作者 单际修 李良璧 匡廷云 《Acta Botanica Sinica》 CSCD 2000年第12期1211-1214,共4页
Linear dichroism (LD) spectroscopy is an important technique in the study of the orientation and organization of pigments in the photosynthetic membrane complexes in vivo and in vitro . In this work, the orient... Linear dichroism (LD) spectroscopy is an important technique in the study of the orientation and organization of pigments in the photosynthetic membrane complexes in vivo and in vitro . In this work, the orientation of the pigments in the isolated photosystem Ⅱ (PSⅡ) sub_core reaction center complexes was analyzed and characterized by means of low temperature absorption and LD spectroscopy. The preparations containing different amounts of CP47 isolated from spinach (Spinacia oleracea L.) chloroplast were used in order to investigate the orientation of pigments in the PSⅡ sub_core CP47/D1/D2/Cyt b_559 (CP47/D1/D2) complexes. Chlorophyll a (Chl a) absorbing at 680 nm in CP47/D1/D2/Cyt b_559 complex showed an orientation of the Q y transition parallel to the membrane plane. It is proposed that there are two forms of β_carotene (β_Car) in CP47/D1/D2/Cyt b_559 complex, denoted as β_Car (Ⅰ) and β_Car (Ⅱ), with different orientations, β_Car (Ⅰ) at 470 and 505 nm is roughly parallel to the membrane plane, and β_Car (Ⅱ) at 460 and 490 nm seems to be perpendicular orientation. Upon the photoinhibitory experiment β_Car (Ⅱ) was found to be photosensitive and easily photodamaged. It also showed that the positive LD signal observed at 680 nm was quite complicated. This signal is tentatively attributed to P680 and some Chl a of antenna in CP47 protein based upon our measurements. 展开更多
关键词 orientation of pigments photosystem linear dichroism spectroscopy PHOTOINHIBITION
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Blue light is more essential than red light for maintaining the activities of photosystem Ⅱ and Ⅰ and photosynthetic electron transport capacity in cucumber leaves 被引量:10
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作者 MIAO Yan-xiu W ANG Xiao-zhuo +2 位作者 G AO Li-hong C HEN Qing-yun QU Mei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第1期87-100,共14页
Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the re... Blue and red lights differently regulate leaf photosynthesis. Previous studies indicated that plants under blue light generally exhibit better photosynthetic characteristics than those under red light. However, the regulation mechanism of related photosynthesis characteristics remains largely unclear. Here, four light qualities treatments (300 μmol m-2 s-1) including white fluorescent light (FL), blue monochromatic light (B, 440 nm), red monochromatic light (R, 660 nm), and a combination of red and blue light (RB, R:B=8:1) were carried out to investigate their effects on the activity of photosystem II (PSII) and photosystem I (PSI), and photosynthetic electron transport capacity in the leaves of cucumber (Cucumis sativus L.) seedlings. The results showed that compared to the FL treatment, the R treatment significantly limited electron transport rate in PSII (ETR11) and in PSI (ETR1) by 79.4 and 66.3%, respectively, increased non-light induced non-photochemical quenching in PSII (q^No) and limitation of donor side in PSI (φND) and reduced most JIP-test parameters, suggesting that the R treatment induced suboptimal activity of photosystems and inhibited electron transport from PSII donor side up to PSI. However, these suppressions were effectively alleviated by blue light addition (RB). Compared with the R treatment, the RB treatment significantly increased ETR, and ETR1 by 176.9 and 127.0%, respectively, promoted photosystems activity and enhanced linear electron transport by elevating electron transport from QA to PSI. The B treatment plants exhibited normal photosystems activity and photosynthetic electron transport capacity similar to that of the FL treatment. It was concluded that blue light is more essential than red light for normal photosynthesis by mediating photosystems activity and photosynthetic electron transport capacity. 展开更多
关键词 CUCUMBER photosystem II photosystem I electron transport
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Light-induced Damage of Photosystem ⅡPrimary Electron Donor P680: a High Performance Liquid Chromatographic Analysis of Pigment Content in D1/D2 /Cytochrome b559 Complex Under Photoinhibitory Conditions 被引量:1
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作者 彭德川 侯建敏 +2 位作者 匡廷云 唐崇钦 汤佩松 《Acta Botanica Sinica》 CSCD 1999年第12期1307-1311,共5页
ByHPLCanalyticalmethod ,thechangeofPSⅡRC’spigmentcontentintheprocessofphoto damageunderstrongilluminationfromspinach (SpinaciaoleraceaMill.)wascomparativelystudied .Theex perimentalresultsshowthat :(1)Inauthors... ByHPLCanalyticalmethod ,thechangeofPSⅡRC’spigmentcontentintheprocessofphoto damageunderstrongilluminationfromspinach (SpinaciaoleraceaMill.)wascomparativelystudied .Theex perimentalresultsshowthat :(1)Inauthors’analyticalconditions ,(ofwhich ,[Chl]=150 μg/mL ,andthe illuminationstrengthwasputat 2 .3× 10 6 mJ·m- 2 ·s- 1) ,4 5minofilluminationcouldcausealmostthewhole lossofA6 80inthefourthderivativeabsorptionspectra ,whileA6 70decreasedtoaboutonehalfofitsoriginal intensity ;theabsorptionmaximuminred ,concurrently ,wasshiftedfrom 6 76nmto 6 71nm ,representingthe lossofmorethan 90 %ofthephotochemicalactivitiesofthePSⅡRC .(2 )Duringtheperiodofcontinuousil lumination ,theChlconcentrationdecreasedina 3_periodstyle ,whichmeantthatthefirst [Chl]decreasedto the 2 / 3ofitsoriginalamountfrom 2 0minto 4 0minafterilluminationhadstarted ,thenbecamestabilizedup toabout 6 0minofillumination ,thereafteraseconddecreaseof [Chl]inanotherabout2 0minuntilitreached about 30 %oftheoriginallevelandremainedunchangedfromabout 80minon .Theoriginalpigmentcompo nentsofD1/D2 /Cytb559wasapproximatelyas 6Chla∶2Pheo∶2 β_Carwhichareinsupportofauthors’pre viousproposalabouttheminimumChl/Pheoratioof 4∶2inPSⅡRC’spigmentcontents .(3)Afterabout 4 0 minofillumination ,anewlyappearedelutionpeakwasfoundbetweenthePheoandβ_CarpeaksinHPLCpro file ,attheretentiontimeof7.2min ,alittlelaterthanthat (6 .9min)ofPheomolecules ,thenewlyappeared elutionpeakwassupposedtobeakindofaccumulatedandstableproductofthePSⅡRC’sphotodamagepro cessandverymuchpossiblethePheo_likemolecules . 展开更多
关键词 photosystem P6 80 Chla D1/D2 /Cytb559 PHOTOINHIBITION HPLC
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The Influence of Surfactants on Chlorophyll Binding Status and Excitation Energy Transfer in Photosystem Ⅰ of Wheat
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作者 郁飞 唐崇钦 +5 位作者 辛越勇 彭德川 李良璧 匡廷云 李济云 李振声 《Acta Botanica Sinica》 CSCD 2001年第7期686-692,共7页
Surfactants are widely used in the purification and research of structure and function of the protein complexes in photosynthetic membrane. To elucidate the mechanism of interaction between surfactants and photosystem... Surfactants are widely used in the purification and research of structure and function of the protein complexes in photosynthetic membrane. To elucidate the mechanism of interaction between surfactants and photosystem Ⅰ (PSⅠ), effects of two typical surfactants, Triton X_100 and sodium dodecyl sulfate (SDS) on PSⅠ, were studied at different concentrations. The results were: SDS led to the reduction of apparent absorption intensity and blue shift of absorption peaks; while Triton X_100 led to the decrease of apparent absorption intensity in red region and blue shift of the peak, but to the increase of apparent absorption intensity in blue region. The fourth derivative spectra show that the longwavelength (669 nm and 683 nm) absorbing chlorophyll a was affected greatly and their relative changes of absorbance were axially symmetrical. The presence of surfactant could make the long wavelength fluorescence emission decrease greatly and a new fluorescence peak appeared around 680 nm, it was obvious that the surfactant interceded the transfer of excitation energy from antenna pigments to reaction center. The surfactants might affect the microenvironment of proteins, even the structure of PSⅠ protein subunits and hence changed the binding status of pigments with protein subunits, or the pigments might be released from the subunits. All of these might affect the absorption and the transfer of excitation energy. 展开更多
关键词 SURFACTANT photosystem CHLOROPHYLL absorption spectra fluorescence spectra
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Comparison Between the Influences of Phosphatidylcholine and Triton X-100 on the Protein Secondary Structures and Oxygen-evolving Activity of Photosystem Ⅱ 被引量:2
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作者 阮翔 许强 +4 位作者 毛海滨 单际修 公衍道1张秀芳 匡廷云 赵南明 《Acta Botanica Sinica》 CSCD 2000年第12期1220-1224,共5页
The structural and functional alterations within the PSⅡ membrane from phosphatidylcholine reconstitution and Triton X_100 (TX_100) treatment were studied by using Fourier transform_infrared (FT_IR) spectroscopic tec... The structural and functional alterations within the PSⅡ membrane from phosphatidylcholine reconstitution and Triton X_100 (TX_100) treatment were studied by using Fourier transform_infrared (FT_IR) spectroscopic technique and oxygen electrode. Phosphatidylcholine reconstitution showed no significant effect on the protein secondary structures of PSⅡ membrane but an increase of the rate of PSⅡ_mediated oxygen_evolution. The phosphatidylcholine lipids with different length of acyl chains displayed different capabilities to stimulate oxygen_evolution. In contrast, perturbation of the bilayer lipids by TX_100 resulted in obvious changes of the protein secondary structures within the PSⅡ membrane and in the loss of the PSⅡ_mediated oxygen_evolving activity. The results indicate the importance of membrane integrity in maintaining the stability of the photosynthetic membrane proteins. 展开更多
关键词 photosystem PHOSPHATIDYLCHOLINE Triton X_100 FT_IR oxygen_evolution
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Effects of CeCl_3 on Energy Transfer and Oxygen Evolution in Spinach Photosystem Ⅱ 被引量:7
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作者 刘晓晴 苏明玉 +3 位作者 刘超 张璐 司文会 洪法水 《Journal of Rare Earths》 SCIE EI CAS CSCD 2007年第5期624-630,共7页
Due to 4f electron characteristics and alternation valence, cerium involved in an oxidation-reduction reaction in plant, closely relating to photosynthesis. Our studies proved that cerium could promote photosynthesis ... Due to 4f electron characteristics and alternation valence, cerium involved in an oxidation-reduction reaction in plant, closely relating to photosynthesis. Our studies proved that cerium could promote photosynthesis and greatly improve spinach growth. However, the mechanism of promoting energy transfer and conversion by cerium remains unclear. Here we reported that the effects of Ce^3+ on energy transfer and oxygen evolution in photosystem Ⅱ (PS Ⅱ ) isolated from spinach, which was related to 4f electron characteristics and alternation valence in Ce molecule. The methods of absorption spectrum, fluorescence spectrum were used in the research. Results showed that Ce^3+ treatment at low concentration could suitably change PS Ⅱ mieroenvironment, increase the absorbance of visible light, improve the energy transfer among amino acids within PS Ⅱ protein-pigment complex, and accelerate energy transport from tyrosine residue to chlorophyll a. In summary, the photochemical activity of PS Ⅱ (fluorescence quantum yield) and its oxygen evolving rate were enhanced by Ce^3+. 展开更多
关键词 CeCla SPINACH photosystem spectral characteristics energy transfer oxygen evolution rare earths
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Effects of short-term low temperatures on photosystem Ⅱ function of samara and leaf of Siberian maple (Acer ginnala) and subsequent recovery 被引量:10
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作者 XiangLiang PAN1, DaoYong ZHANG1,2, Xi CHEN1, Li LI1, GuiJin MU1, LanHai LI1, AnMing BAO1, Jing LIU2, HuaiSong ZHU1, WenJuan SONG1, JianYing YANG1, JunYong AI1 1 Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China 2 State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550000, China 《Journal of Arid Land》 SCIE 2009年第1期57-63,共7页
Samara is the reproductive organ (seed) for many tree species in arid land in northwestern China. It is ecologically important in population development due to its dispersal function. However, information on its photo... Samara is the reproductive organ (seed) for many tree species in arid land in northwestern China. It is ecologically important in population development due to its dispersal function. However, information on its photosynthesis and effect of environmental stresses on its photosynthesis is still very limited. In the present study, responses of photosystem II (PSII) activity in samara and leaf of Siberian maple to short-term chilling/freezing and subsequent recovery potential were comparatively investigated by using polyphasic fluorescence test. The samara had more efficient photosynthesis (Fv/Fm and PIABS) and more efficient electron transport (φEo) but lower energy dis- sipation (DIo/RC) than leaf. Generally, the PSII performance and the electron transport for both samara and leaf were inhibited under low temperature stress, accompanied by an increase of energy dissipation in PSII reaction centers (RCs). PSII of both samara and leaf was not markedly affected by chilling and could acclimate to chilling stress. Short-term freezing could completely inhibit PSII activity in both samara and leaf, indicated by the drop of values of Fv/Fm, PIABS, φEo to zero. PSII functional parameters of short-term dark frozen samara could be largely recovered whereas those of frozen leaf could not be recovered. The higher tolerance of samara to short-term low temperature stress than leaf is of great ecological significance for seed development, population establishment of Siberian maple. 展开更多
关键词 samara photosystem JIP-test analysis chlorophyll fluorescence
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Effect of Nano-anatase TiO_2 on Spectral Characterization of PhotosystemⅡParticles from Spinach 被引量:10
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作者 HONGFa-shui YANGPing +4 位作者 GAOFeng-qing LIUChao ZHENGLei YANGFan ZHOUJuan 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2005年第2期196-200,共5页
The photosystem Ⅱ(PSⅡ) particles were purified by means of nano-anatase TiO_2 treatment of spinach and studied by spectroscopy. The results show that the electron transport and the oxygen-evolving rate of PSⅡ are a... The photosystem Ⅱ(PSⅡ) particles were purified by means of nano-anatase TiO_2 treatment of spinach and studied by spectroscopy. The results show that the electron transport and the oxygen-evolving rate of PSⅡ are accelerated after it has been treated with nano-anatase TiO_2; the UV-Vis absorption spectrum of PSⅡ particles is increased; the red shift of fluorescence emission peak of PSⅡ is 2 nm; the peak intensity is decreased; the PSⅡ signal Ⅱs of low temperature electron paramagnetic resonanace(EPR) spectrum is intensified under light, and the PSⅡ circular dichroism(CD) spectrum is similar to that of control. It is suggested that nano-anatase TiO_2 might bind to the PSⅡ reaction center complex and intensify the function of the PSⅡ electron donor, however, nano-anatase TiO_2 treatment does not change the configuration of the PSⅡ reaction center complex. 展开更多
关键词 Nano-anatase TiO_2 SPINACH photosystem Spectral characterization
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EXCITATION ENERGY TRANSFER IN VITRO BETWEEN PHYCOBILIPROTEINS AND THYLAKOID PHOTOSYSTEM Ⅱ OF HIGHER PLANTS 被引量:1
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作者 仵小南 曾呈奎 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1992年第4期340-346,共7页
The excitation energy transfer from phycobiliproteins to thylakoid PSII of higher plants was investigated. When incubated with spinach thylakoids, phycobiliproteins isolated from red and blue- green algae transferred ... The excitation energy transfer from phycobiliproteins to thylakoid PSII of higher plants was investigated. When incubated with spinach thylakoids, phycobiliproteins isolated from red and blue- green algae transferred light energy absorbed to spinach PSII. The efficiency of energy transfer was dependent on the kind of phycobiliproteins used. If spinach thylakoids were replaced by the thylakoids of Brassica chinensis, R phycoerythin or C- phycocyanin did not transfer their excitation energy to PSII of Brassica chinensis unless allophycocyanin was present. 展开更多
关键词 PHYCOBILIPROTEINS HIGHER plants photosystem II energy transfer BIOREACTOR
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Blocking backward reaction on hydrogen evolution cocatalyst in a photosystem Ⅱ hybrid Z-scheme water splitting system 被引量:1
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作者 Zhen Li Yu Qi +6 位作者 Wangyin Wang Deng Li Zheng Li Yanan Xiao Guangye Han Jian-Ren Shen Can Li 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2019年第4期486-494,M0001,共10页
Photocatalytic Z-scheme water splitting is considered as a promising approach to produce solar hydrogen.However,the forward hydrogen production reaction is often impeded by backward reactions.In the present study,in a... Photocatalytic Z-scheme water splitting is considered as a promising approach to produce solar hydrogen.However,the forward hydrogen production reaction is often impeded by backward reactions.In the present study,in a photosystem Ⅱ-integrated hybrid Z-scheme water splitting system,the backward hydrogen oxidation reaction was significantly suppressed by loading a PtCrOx cocatalyst on a ZrO2/TaON photocatalyst.Due to the weak chemisorption and activation of molecular hydrogen on PtCrOx,where Pt is stabilized in the oxidized forms,Pt^Ⅱ and Pt^Ⅳ,hydrogen oxidation is inhibited.However,it is remarkably well-catalyzed by the metallic Pt cocatalyst,thereby rapidly consuming the produced hydrogen.This work describes an approach to inhibit the backward reaction in the photosystem Ⅱ-integrated hybrid Z-scheme water splitting system using Fe(CN)6^3-/Fe(CN)6^4-redox couple as an electron shuttle. 展开更多
关键词 Water splitting COCATALYST Backward reaction Hydrogen oxidation photosystem
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Reduced salinity interacts with ultraviolet radiation to alter photosystem Ⅱ function in diatom Skeletonema costatum 被引量:1
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作者 Shasha ZANG Fang YAN +4 位作者 Daode YU Jingjing SONG Lei WANG Zhiguang XU Hongyan WU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2022年第4期1615-1627,共13页
To investigate the effect of reduced salinity on diatoms’ capacity to cope with changing ultraviolet radiation(U VR) and photosynthetically active radiation(PAR),Skeletonema costatum was grown in a range of salinity(... To investigate the effect of reduced salinity on diatoms’ capacity to cope with changing ultraviolet radiation(U VR) and photosynthetically active radiation(PAR),Skeletonema costatum was grown in a range of salinity(15,25,and 35).The photo system Ⅱ(PSⅡ) function was analyzed by increasing PAR and UVR to mimic a mixing event in turbulent waters.The re sults show that high UVR exposure significantly reduced PSII activity,especially in cells grown at low salinity.UVR,but not salinity,stimulated the ’removal’ rate of PSII protein PsbA.Salinity alone,in the range of 15 to 35,did not regulate PSⅡ acceptor region;however,the low salinity+UVR treatment decreased the energy flux for electron transport per PSⅡ reaction center in S.costatum.It showed that low salinity exacerbated the damaging effect of UVR on PSⅡ function in S.costatum by suppressing Psb A protein synthe sis and modifying the photochemistry of PSⅡ.Although higher catalase(CAT) activity and NPQs were induced,they were unable to prevent the combined damage effect of low salinity+UVR.Our findings indicate that reduced salinity and increased UVR potentially affect the abundance and distribution of S.costatum with the escalation of climate disturbances. 展开更多
关键词 DIATOM ultraviolet radiation(UVR) PHOTOINACTIVATION photosystem II
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Photoinduced hydrogen evolution in an artificial system containing photosystem I, hydrogenase, methy1 viologen and mercaptoacetic acid
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作者 Dong Jin Qian mi Rong Liu +2 位作者 Chikashi Nakamura Stephan Olav Wenk Jun Miyake 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第5期607-610,共4页
Hydrogen evolution was detected in an artificial system composed of light-harvesting unit of purified photosystem I, catalyst of hydrogenase, methyl viologen and electron donor under radiation. Absorption spectral fea... Hydrogen evolution was detected in an artificial system composed of light-harvesting unit of purified photosystem I, catalyst of hydrogenase, methyl viologen and electron donor under radiation. Absorption spectral features confirmed that electron transfer from electron donors to proton was via a photoinduced reductive process of methyl viologen. 展开更多
关键词 Hydrogen evolution HYDROGENASE photosystem I Artificial system
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Analysis of Chloroplast Ultrastructure,Photosystem Ⅱ Light Harvesting Complexes and Chlorophyll Synthesis in a Chlorophyll-Less Rice Mutant W2555
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作者 Xu Pei-zhou LI Yun +4 位作者 YUAN Shu ZHANG Hong-yu WANG Xu-dong LIN Hong-hui Wu Xian-jun 《Rice science》 SCIE 2006年第4期265-270,共6页
A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ (LHC Ⅱ) was conducted between a new rice mutant (W2555) and its wild type (WT). The chloroplasts of W2555 had les... A comparative study on chloroplast ultrastructure and light harvesting complex of photosystem Ⅱ (LHC Ⅱ) was conducted between a new rice mutant (W2555) and its wild type (WT). The chloroplasts of W2555 had less thylakoids and grana stacks compared with the wild type. There was no significant change in the composition of LHC Ⅱ polypeptide in W2555, while a decline had been noted in LHC Ⅱ content. Northern blot analysis with a specific cab gene probe showed no appreciable difference in the LHC Ⅱ mRNA level between the W2555and its wild type. The precursors of chlorophyll synthesis, 6-aminolevulinic acid (ALA) and porphobilinogen (PBG) were over accumulated in W2555, but the other precursors were all decreased. These results indicated that the decreased level of LHC Ⅱ in the mutant W2555 was attributed to the change of cab gene transcription, but a blockage in chlorophyll biosynthesis due to the formation of uroporphyrinogen Ⅲ (Urogen Ⅲ). 展开更多
关键词 chloroplast ultrastructure photosystem chlorophyll synthesis precursors chlorophyll-less mutant RICE
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Responses of Maximum Photosystem H PhotochemicalEfficiency of Phytoplankton Communities to NutrientLimitation in the Coastal Sea of Qingdao, China
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《Journal of Ocean University of China》 SCIE CAS 2014年第1期83-90,共8页
Nutrient enrichment experiments with nitrogen (N) and phosphorus (P) were conducted with samples from two stationsin the coastal waters of Qingdao, China, during summer to identify limiting nutrients. In late July... Nutrient enrichment experiments with nitrogen (N) and phosphorus (P) were conducted with samples from two stationsin the coastal waters of Qingdao, China, during summer to identify limiting nutrients. In late July of 2009, low P concentrations andthe maximum photochemical efficiency of photosystem II (Fv/Fm) in the initial samples together with Fv/Fm and chlorophyll a (Chl a)responses to P addition indicated P limitation at the two stations. In early August, low P levels still limited phytoplankton growth atstation A. Fv/Fm and Chl a were the highest in the NP treatments at station B, suggesting an N/P co-limitation. In mid-September,nutrient concentrations and Fv/Fm were elevated and phytoplankton communities were healthy. Greater Fv/Fm and Chl a in the treat-ments with added P than those without the addition suggested potential P limitation at station A. Lack of Fv/Fm and Chl a responsesfollowing nutrient additions indicated N and P repletion at station B. At the end of July 2010, neither N nor P was limited at station B.Additionally, Fv/Fm coupled with 24-h-long nutrient enrichment experiments can be used to detect P limitation and N/P co-limitationto natural populations. This method can be more accurate for assessing co-limitation than the use of criteria of nutrient concentrationsand ratios as indicators, and can provide more rapid results than nutrient addition bioassays using chlorophyll response as an indica-tor, when a population is potentially limited. Compared with the two conventional methods, the results based on F,/F~ can also pro-vide more detailed information about physiological states of the populations. 展开更多
关键词 Key words MAXIMUM PHOTOCHEMICAL efficiency of photosystem II (Fv Fm) nutrient limitation nitrogen phosphorus phyto-plankton
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Effects of Severe Drought and Glyphosate Stress on Physiological Characteristics and Protein Expression of Photosystem Ⅱ in Genentically Modified Soybean
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作者 Chang Lijuan Liu Wenjuan Zhang Fuli 《Plant Diseases and Pests》 CAS 2019年第2期22-26,共5页
[Objective] The paper was to investigate effects of glyphosate stress on physiological characteristics and protein expression of photosystem Ⅱ(PSⅡ) in genentically modified soybean GTS 40-3-2 seedlings under severe ... [Objective] The paper was to investigate effects of glyphosate stress on physiological characteristics and protein expression of photosystem Ⅱ(PSⅡ) in genentically modified soybean GTS 40-3-2 seedlings under severe drought condition. [Method] A pot experiment was carried out in growth chamber to determine the response of genetically modified soybean treated by severe drought stress and different concentrations of glyphosate at the third compound leaf stage. [Result] Severe drought treatment increased the electrolyte leakage(EL), superoxide dismutase(SOD) and peroxidase(POD) activities, and decreased the relative water content(RWC), chlorophyll content, and catalase(CAT) activity. The EL, SOD and POD activities were significantly increased in severe drought and glyphosate treatments, which were related to glyphosate concentrations. The chlorophyll content decreased, which was also related to glyphosate concentrations. But the BWC and CAT activity were not affected by glyphosate concentrations. Western blot displayed that PSⅡ protein Lhcb2 was not affected by stress conditions and stably expressed. D1, D2 and Lhcb4 protein level decreased, and there was no significant change in Lhcb1 expression under severe drought stress. The protein levels of D1, D2, Lhcb1 and Lhcb4 decreased with the increase of glyphosate concentrations under severe drought and glyphosate stress. When the glyphosate concentrations were 0.92 and 1.84 kg·ai/hm^2, the protein levels of D1, D2 and Lhcb4 were slightly higher than those in severe drought stress. When the glyphosate concentrations were 3.68 and 7.36 kg·ai/hm^2, the protein level of D1, D2, Lhcb1 and Lhcb4 decreased sharply. [Conclusion] This research provides a theoretical basis for production of genetically modified soybean. 展开更多
关键词 Severe drought stress Glayphosate Genetically modified soybean photosystem
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Optimum Dark Adaptation Period for Evaluating the Maximum Quantum Efficiency of Photosystem II in Ozone-Exposed Rice Leaves
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作者 Hiroki Hiroki Kobayakawa Katsu Imai 《American Journal of Plant Sciences》 2013年第9期1750-1757,共8页
Because the transient O3 injury of leaves is lost with time, the evaluation of O3 effect on the maximum quantum efficiency of PSII (Fv/Fm) is difficult. Thus, the authors examined Fv/Fm in rice leaves exposed to diffe... Because the transient O3 injury of leaves is lost with time, the evaluation of O3 effect on the maximum quantum efficiency of PSII (Fv/Fm) is difficult. Thus, the authors examined Fv/Fm in rice leaves exposed to different O3 concentrations (0, 0.1, and 0.3 cm3·m-3, expressed as O0, O0.1, and O0.3) under different dark adaptation periods (0, 1, 5, 10, 20, and 30 min, expressed as D0, D1, D5, D10, D20, and D30) to ascertain its optimum time span. Fv/Fm was inhibited by O3;however in the O0 and O0.1 plants, it recovered during dark adaptation. In the O0.3 plants, Fv/Fm decreased gradually with time. F0 was found to be increased by O3, and it increased further in the O0.3 plants during dark adaptation. Under a high light intensity, Fm was decreased by O3, and the O3-induced damage to Fv/Fm was therefore more pronounced. However, the sensitivity of 展开更多
关键词 Dark-Adapted State ORYZA SATIVA OZONE Stress photosystem II Quantum Efficiency
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