Objective: To investigate the expression of p16, cyclin D1 and Rb protein in gastric carcinoma and premalignant lesions including dysplastic gastric mucosa and intestinal metaplasia gastric mucosa. Methods: Using SP i...Objective: To investigate the expression of p16, cyclin D1 and Rb protein in gastric carcinoma and premalignant lesions including dysplastic gastric mucosa and intestinal metaplasia gastric mucosa. Methods: Using SP immunohistochemical methods, the expression of pl6, cyclin D1 and Rb proteins was detected in 10 specimens of normal gastric mucosa, 15 specimens of dysplastic gastric mucosa, 15 specimens of intestinal metaplasia gastric mucosa, 30 specimens of gastric carcinoma. The clinical characteristics of the 30 patients with gastric carcinoma were analysed to explore the relationship between the parameter detected and biological action of gastric cancer. Results: Expression of p16 protein was detected in 90% of normal gastric mucosa, 86.67% of dysplastic gastric mucosa, 86.67% of intestinal metaplasia gastric mucosa, 36.67% of gastric carcinoma. The positive rate of p16 protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.01). Expression of cyclin D1 protein was detected in 10% of normal gastric mucosa, 20% of dysplastic gastric mucosa, 20% of intestinal metaplasia gastric mucosa, 53.33% of gastric carcinoma. The positive rate of cyclin D1, protein expression in gastric carcinoma is significantly higher than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.05). Expression of Rb protein was detected in 90% of normal gastric mucosa, 80% of dysplastic gastric mucosa, 80% of intestinal metaplasia gastric mucosa, 50% of gastric carcinoma. The positive rate of Rb protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa (P<0.05). The expression of p16, cyclin D1 gene were associated with the degree of differentiation of gastric carcinoma, lymphnodes metastasis and distant metastasis. Conclusion: p16, Cyclin D1 and Rb gene play important role in gastric carcinoma genesis. The expression of p16, cyclin D1 and Rb gene have some value to the diagnosis at earlier stage of gastric cancer. Detection of expression of p16, cyclin D1 gene would be helpful to judge the prognosis of gastric cancer.展开更多
Objective:The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylated RB protein and proliferating cell nuclear antigen(PCNA) in human breast cancer.Methods:MTT colorimetric as...Objective:The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylated RB protein and proliferating cell nuclear antigen(PCNA) in human breast cancer.Methods:MTT colorimetric assay was applied to examine the growth inhibition,and the apoptosis was determined by flow cytometry(FCM).The expressing quantity of dephosphorylated RB protein and PCNA pre-and post the action of ADR were detected with immunocytochemistry.Results:MTT assay revealed that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration(IC50) value was 0.128 mg/L.Tumor cell apoptotic rate(AR) in ADR group(χ= 0.259) was significantly higher than that in the control group(χ = 0.045)(P < 0.01).The expressive levels of dephosphorylated RB protein in ADR group(MOD × area = 986.8 ± 207.4) was significantly higher than that in control group(MOD × area =131.7 ± 31.9)(P < 0.01).PCNA positive expression rate in ADR group(χ = 0.3371) was significantly lower than that in the control group(χ = 0.5152)(P < 0.01).Conclusion:In ADR group,there was significant positive correlation between AR and the expressing quantity of dephosphorylated RB protein,but there was significant negative correlation between AR and PCNA.展开更多
The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallb...The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line （Mz-ChA-1） were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.展开更多
目的:研究RB蛋白表达状况对乳腺癌细胞凋亡及其周期变化的影响。方法:以乳腺癌细胞株MCF-7/S为研究对象,采用免疫细胞化学法检测多柔比星(ADR)作用前后RB蛋白的表达状态;应用流式细胞术进行细胞凋亡和细胞周期分析。结果:加ADR前MCF-7/...目的:研究RB蛋白表达状况对乳腺癌细胞凋亡及其周期变化的影响。方法:以乳腺癌细胞株MCF-7/S为研究对象,采用免疫细胞化学法检测多柔比星(ADR)作用前后RB蛋白的表达状态;应用流式细胞术进行细胞凋亡和细胞周期分析。结果:加ADR前MCF-7/S细胞的细胞核磷酸化RB蛋白为阳性,去磷酸化RB蛋白为阴性。经不同浓度ADR处理后,随着ADR浓度的增加,MCF-7/S细胞去磷酸化RB蛋白表达量逐渐增高。流式细胞术分析结果表明,经0.25、2.0和5.0μg/m l ADR作用24 h后,MCF-7/S细胞凋亡率分别为17.1%、18.4%和25.9%。细胞周期分析表明,MCF-7/S细胞主要积聚于G1期。结论:去磷酸化RB蛋白表达与MCF-7/S细胞凋亡率呈正相关(r=0.998,P<0.05),同时MCF-7/S积聚于G1期。展开更多
文摘Objective: To investigate the expression of p16, cyclin D1 and Rb protein in gastric carcinoma and premalignant lesions including dysplastic gastric mucosa and intestinal metaplasia gastric mucosa. Methods: Using SP immunohistochemical methods, the expression of pl6, cyclin D1 and Rb proteins was detected in 10 specimens of normal gastric mucosa, 15 specimens of dysplastic gastric mucosa, 15 specimens of intestinal metaplasia gastric mucosa, 30 specimens of gastric carcinoma. The clinical characteristics of the 30 patients with gastric carcinoma were analysed to explore the relationship between the parameter detected and biological action of gastric cancer. Results: Expression of p16 protein was detected in 90% of normal gastric mucosa, 86.67% of dysplastic gastric mucosa, 86.67% of intestinal metaplasia gastric mucosa, 36.67% of gastric carcinoma. The positive rate of p16 protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.01). Expression of cyclin D1 protein was detected in 10% of normal gastric mucosa, 20% of dysplastic gastric mucosa, 20% of intestinal metaplasia gastric mucosa, 53.33% of gastric carcinoma. The positive rate of cyclin D1, protein expression in gastric carcinoma is significantly higher than that in normal gastric mucosa and gastric premalignant lesions mucosa (P<0.05). Expression of Rb protein was detected in 90% of normal gastric mucosa, 80% of dysplastic gastric mucosa, 80% of intestinal metaplasia gastric mucosa, 50% of gastric carcinoma. The positive rate of Rb protein expression in gastric carcinoma is significantly lower than that in normal gastric mucosa (P<0.05). The expression of p16, cyclin D1 gene were associated with the degree of differentiation of gastric carcinoma, lymphnodes metastasis and distant metastasis. Conclusion: p16, Cyclin D1 and Rb gene play important role in gastric carcinoma genesis. The expression of p16, cyclin D1 and Rb gene have some value to the diagnosis at earlier stage of gastric cancer. Detection of expression of p16, cyclin D1 gene would be helpful to judge the prognosis of gastric cancer.
文摘Objective:The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylated RB protein and proliferating cell nuclear antigen(PCNA) in human breast cancer.Methods:MTT colorimetric assay was applied to examine the growth inhibition,and the apoptosis was determined by flow cytometry(FCM).The expressing quantity of dephosphorylated RB protein and PCNA pre-and post the action of ADR were detected with immunocytochemistry.Results:MTT assay revealed that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration(IC50) value was 0.128 mg/L.Tumor cell apoptotic rate(AR) in ADR group(χ= 0.259) was significantly higher than that in the control group(χ = 0.045)(P < 0.01).The expressive levels of dephosphorylated RB protein in ADR group(MOD × area = 986.8 ± 207.4) was significantly higher than that in control group(MOD × area =131.7 ± 31.9)(P < 0.01).PCNA positive expression rate in ADR group(χ = 0.3371) was significantly lower than that in the control group(χ = 0.5152)(P < 0.01).Conclusion:In ADR group,there was significant positive correlation between AR and the expressing quantity of dephosphorylated RB protein,but there was significant negative correlation between AR and PCNA.
文摘The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line （Mz-ChA-1） were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.
文摘目的:研究RB蛋白表达状况对乳腺癌细胞凋亡及其周期变化的影响。方法:以乳腺癌细胞株MCF-7/S为研究对象,采用免疫细胞化学法检测多柔比星(ADR)作用前后RB蛋白的表达状态;应用流式细胞术进行细胞凋亡和细胞周期分析。结果:加ADR前MCF-7/S细胞的细胞核磷酸化RB蛋白为阳性,去磷酸化RB蛋白为阴性。经不同浓度ADR处理后,随着ADR浓度的增加,MCF-7/S细胞去磷酸化RB蛋白表达量逐渐增高。流式细胞术分析结果表明,经0.25、2.0和5.0μg/m l ADR作用24 h后,MCF-7/S细胞凋亡率分别为17.1%、18.4%和25.9%。细胞周期分析表明,MCF-7/S细胞主要积聚于G1期。结论:去磷酸化RB蛋白表达与MCF-7/S细胞凋亡率呈正相关(r=0.998,P<0.05),同时MCF-7/S积聚于G1期。
