The immobilization of acetylcholinesterase （ACHE） on amino functionalized SBA-15 mesoporous sieves （NH2-SBA-15） was explored for detection of organophosphorus and carhamate pesticides. Several methods have been st...The immobilization of acetylcholinesterase （ACHE） on amino functionalized SBA-15 mesoporous sieves （NH2-SBA-15） was explored for detection of organophosphorus and carhamate pesticides. Several methods have been studied and the ＂adsorption-crosslinking＂ is found to be the best one. Firstly, AChE and bovine serum albumin （BSA） were adsorbed in turn on the surface of NH2-SBA-15 by electrostatic adsorption, and then glutaraldehyde was added for cross-linking the amino group of AChE. The NH2-SBA- 15 showed around 95% immobilization efficiency and the specific activity of immobilized AChE （ACHE- NH2-SBA-15） reached 130% relative to free AChE under optimal immobilization conditions. It was found AChE-NH2-SBA-15 retained 94.0% and 82.8% of its initial activity after 60days at 4℃ and 25℃, respectively. It remained 55.0% of its initial activity after 6 times recycling. During the detection of pesticide, the AChE-NH2-SBA-15 showed wider linear range and much lower limit of detection compared with free ACHE. The current method showed good recovery （93.8%-109.3%） and low RSD （〈5%） for both standard solution and real vegetable samples of Carbaryl and Trichlorfon. It was believed that AChE-NH2- SBA- 15 could be exploited as a fast, sensitive and low-cost biocatalyst towards the detection of pesticides residues which could be stored at room temperature for a long time.展开更多
The biggest challenge for organic phase change materials(PCMs)used in cold energy storage is to maintain high heat storage capacity while reducing the leakage risk of PCMs during the phase transition process.This is c...The biggest challenge for organic phase change materials(PCMs)used in cold energy storage is to maintain high heat storage capacity while reducing the leakage risk of PCMs during the phase transition process.This is crucial for expanding their applications in the more demanding cold storage field.In this study,novel formstable low-temperature composite PCMs are prepared with mesoporous materials,namely SBA-15 and CMK-3(which are prepared using the template method),as supporting matrices and dodecane as the PCM.Owing to the combined effects of capillary forces within mesoporous materials and interactions among dodecane molecules,both dodecane/SBA-15 and dodecane/CMK-3 exhibit outstanding shape stability and thermal cycling stability even after 200 heating/cooling cycles.In comparison to those of dodecane/SBA-15,dodecane/CMK-3 exhibits superior cold storage performance and higher thermal conductivity.Specifically,the phase transition temperature of dodecane/CMK-3 is-8.81℃ with a latent heat of 122.4 J·g^(-1).Additionally,it has a thermal conductivity of 1.21 W·m^(-1)·K^(-1),which is 9.45 times that of dodecane alone.All these highlight its significant potential for applications in the area of cold energy storage.展开更多
基金supported by the National Natural Science Foundation of China (No.21303105)Shanghai Committee of Science and Technology (No.13430503400)
文摘The immobilization of acetylcholinesterase （ACHE） on amino functionalized SBA-15 mesoporous sieves （NH2-SBA-15） was explored for detection of organophosphorus and carhamate pesticides. Several methods have been studied and the ＂adsorption-crosslinking＂ is found to be the best one. Firstly, AChE and bovine serum albumin （BSA） were adsorbed in turn on the surface of NH2-SBA-15 by electrostatic adsorption, and then glutaraldehyde was added for cross-linking the amino group of AChE. The NH2-SBA- 15 showed around 95% immobilization efficiency and the specific activity of immobilized AChE （ACHE- NH2-SBA-15） reached 130% relative to free AChE under optimal immobilization conditions. It was found AChE-NH2-SBA-15 retained 94.0% and 82.8% of its initial activity after 60days at 4℃ and 25℃, respectively. It remained 55.0% of its initial activity after 6 times recycling. During the detection of pesticide, the AChE-NH2-SBA-15 showed wider linear range and much lower limit of detection compared with free ACHE. The current method showed good recovery （93.8%-109.3%） and low RSD （〈5%） for both standard solution and real vegetable samples of Carbaryl and Trichlorfon. It was believed that AChE-NH2- SBA- 15 could be exploited as a fast, sensitive and low-cost biocatalyst towards the detection of pesticides residues which could be stored at room temperature for a long time.
基金supported by the National Natural Science Foundation of China(Grant No.51906230)the Key scientific and technological projects in Henan Province(Grant No.212102210007)the Project of Zhongyuan Science and Technology Innovation Talents(Grant No.234200510011).
文摘The biggest challenge for organic phase change materials(PCMs)used in cold energy storage is to maintain high heat storage capacity while reducing the leakage risk of PCMs during the phase transition process.This is crucial for expanding their applications in the more demanding cold storage field.In this study,novel formstable low-temperature composite PCMs are prepared with mesoporous materials,namely SBA-15 and CMK-3(which are prepared using the template method),as supporting matrices and dodecane as the PCM.Owing to the combined effects of capillary forces within mesoporous materials and interactions among dodecane molecules,both dodecane/SBA-15 and dodecane/CMK-3 exhibit outstanding shape stability and thermal cycling stability even after 200 heating/cooling cycles.In comparison to those of dodecane/SBA-15,dodecane/CMK-3 exhibits superior cold storage performance and higher thermal conductivity.Specifically,the phase transition temperature of dodecane/CMK-3 is-8.81℃ with a latent heat of 122.4 J·g^(-1).Additionally,it has a thermal conductivity of 1.21 W·m^(-1)·K^(-1),which is 9.45 times that of dodecane alone.All these highlight its significant potential for applications in the area of cold energy storage.
