MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility...MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility of MTs as a biomarker of Sb pollution. A contact method of filter paper was used to investigate Sb inducing MTs in Eiseniafoetida. Spectrophotometrical and SDS-PAGE （sodium dodecyl sulphate-polyacrylamide gel electropheresis） analyses were used to determine the nature of the induced MTs samples. Rabbit liver metallothioneins was used as a standard. The results showed that the molecular weight of induced protein was approximately 6-7 kDa. A characteristic absorption peak at 268 nm was observed, which was consistent with the standard. The amounts of Sb-MTs quantified by cadmium hemoglobin saturation method showed a significant positive relationship with increasing Sb exposure.The experiments prove that Sb can induce Sb-MTs in Eiseniafoetida as a biomarker of antimony pollution.展开更多
A sensitive atomic spectrometric method for the redox speciation analysis of Sb in water is described. The proposed method is based on the selective generation of stibine from Sb（III） in a continuous flow system usi...A sensitive atomic spectrometric method for the redox speciation analysis of Sb in water is described. The proposed method is based on the selective generation of stibine from Sb（III） in a continuous flow system using non-dispersive atomic fluorescence spectrometry for detection. The effects of the HCI concentration on the fluorescence intensities of Sb（III） and Sb（V） were investigated. The results indicated that atomic fluorescence emission due to Sb（V） can constructively interfere with the determination of Sb（III）. For the determination of Sb（III）, four compounds were tested as masking agents to inhibit the generation of stibine from Sb（V）. The effects of the concentrations of the masking agents and of HC1 on the fluorescence signals from Sb（III） and Sb（V） were studied. The results indicated that citric acid and NaF can successfully suppress hydride generation from Sb（V）. To evaluate the developed methodology and the influence of the matrix, the recovery of Sb（III） from natural water that was spiked with different Sb（III） and Sb（V） concentrations was tested.展开更多
文摘MTs （Eiseniafoetida metallothioneins） have been recently reported as biomarkers for environmental metal contamination, however no study regarding Sb （antimony） is available. This study aimed to explore possibility of MTs as a biomarker of Sb pollution. A contact method of filter paper was used to investigate Sb inducing MTs in Eiseniafoetida. Spectrophotometrical and SDS-PAGE （sodium dodecyl sulphate-polyacrylamide gel electropheresis） analyses were used to determine the nature of the induced MTs samples. Rabbit liver metallothioneins was used as a standard. The results showed that the molecular weight of induced protein was approximately 6-7 kDa. A characteristic absorption peak at 268 nm was observed, which was consistent with the standard. The amounts of Sb-MTs quantified by cadmium hemoglobin saturation method showed a significant positive relationship with increasing Sb exposure.The experiments prove that Sb can induce Sb-MTs in Eiseniafoetida as a biomarker of antimony pollution.
文摘A sensitive atomic spectrometric method for the redox speciation analysis of Sb in water is described. The proposed method is based on the selective generation of stibine from Sb（III） in a continuous flow system using non-dispersive atomic fluorescence spectrometry for detection. The effects of the HCI concentration on the fluorescence intensities of Sb（III） and Sb（V） were investigated. The results indicated that atomic fluorescence emission due to Sb（V） can constructively interfere with the determination of Sb（III）. For the determination of Sb（III）, four compounds were tested as masking agents to inhibit the generation of stibine from Sb（V）. The effects of the concentrations of the masking agents and of HC1 on the fluorescence signals from Sb（III） and Sb（V） were studied. The results indicated that citric acid and NaF can successfully suppress hydride generation from Sb（V）. To evaluate the developed methodology and the influence of the matrix, the recovery of Sb（III） from natural water that was spiked with different Sb（III） and Sb（V） concentrations was tested.
