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基于SCAR分子标记和倍性鉴定兰属花卉的研究
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作者 罗富寻 杨小丹 +4 位作者 李枝林 和凤美 瞿素萍 李叶芳 王玉英 《江苏农业科学》 北大核心 2024年第13期31-38,共8页
基于特异性片段扩增(SCAR)和倍性鉴定兰属花卉,为杂交育种亲本选配奠定基础,以兰属花卉的27个大花蕙兰和31个国兰品种为试材,通过SCAR分子标记和流式细胞仪进行亲缘关系和倍性检测,构建系统进化树。SCAR分子标记构建的系统进化树结果显... 基于特异性片段扩增(SCAR)和倍性鉴定兰属花卉,为杂交育种亲本选配奠定基础,以兰属花卉的27个大花蕙兰和31个国兰品种为试材,通过SCAR分子标记和流式细胞仪进行亲缘关系和倍性检测,构建系统进化树。SCAR分子标记构建的系统进化树结果显示:58个品种聚为3支,第1支包括8个大花蕙兰品种(福娘、523、红袍等)和16个国兰品种(碧龙红素、汗血宝马、出水芙蓉等),第2支为11个大花蕙兰品种(金玉满堂、日本香兰、红双喜等)和6个国兰品种(一代天骄、春兰麻壳素、如意素荷等),第3支包括8个大花蕙兰品种(616、黄金岁月、杨贵妃等)和9个国兰品种(碧龙奇莲、西蜀道光、大雪素等);倍性鉴定结果显示:27个大花蕙兰品种中有7个二倍体(日本樱花、绿翡翠、梦境等)、16个三倍体(黄金岁月、蝶影、日本香兰等)、4个四倍体(英雄、523、福娘等)。部分大花蕙兰和国兰品种亲缘关系较近,可用作杂交育种亲本,但由于大花蕙兰倍性较为丰富。因此,大花蕙兰作为杂交育种亲本,选配时需进行倍性鉴定。综上,SCAR分子标记能高效、全面和精准鉴别兰属花卉间的亲缘关系,结合倍性鉴定,可为高效杂交育种奠定基础。 展开更多
关键词 兰属花卉 scar分子标记 倍性鉴定 亲本选配 大花蕙兰
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基于sCARS的淮北平原土壤有机质含量高光谱建模
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作者 高迎凤 赵明松 +2 位作者 于芝琳 赵治东 王涛 《安徽师范大学学报(自然科学版)》 2024年第3期255-263,共9页
为确定淮北平原砂姜黑土土壤有机质(SOM)最佳反演模型,探寻最佳特征波长筛选方法,提高模型预测精度。利用原始光谱进行倒数对数(Log(1/R))、标准正态变量变换(SNV)、去包络线(CR)、一阶微分(FDR)处理,采用稳定竞争性自适应重加权算法(sC... 为确定淮北平原砂姜黑土土壤有机质(SOM)最佳反演模型,探寻最佳特征波长筛选方法,提高模型预测精度。利用原始光谱进行倒数对数(Log(1/R))、标准正态变量变换(SNV)、去包络线(CR)、一阶微分(FDR)处理,采用稳定竞争性自适应重加权算法(sCARS)筛选特征变量,对比分析竞争性自适应重加权算法(CARS)、相关系数法(|r|≥0.47)和显著性水平法(p≤0.01)所得结果,建立SOM含量的偏最小二乘(PLSR)模型,并对比精度差异。结果表明:(1)全波段范围内,SOM含量与原始光谱呈极显著负相关,与Log(1/R)光谱呈极显著正相关,与SNV光谱相关性明显增强。CR和FDR光谱与SOM含量呈不同程度的正负相关性。(2)对比全波段,CARS和sCARS算法能够有效去除光谱冗余信息,筛选得到特征波段数目仅占全波段的1%~5%。筛选后模型精度更高,相对分析误差(RPD)均大于1.8。(3)相比于CARS算法,sCARS算法具备更好的稳定性和精确性。筛选到的特征波段主要分布在800~850、1850~1900、2050~2500 nm区域。(4)Log(1/R)-sCARS模型精度最佳,建模集和预测集的决定系数(R2)分别提升了0.08和0.28,RPD值为3.05,对SOM含量预测极好。 展开更多
关键词 土壤有机质 砂姜黑土 光谱变换 scarS筛选 偏最小二乘模型
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基于SCAR标记和DNA条形码技术的苍术基原鉴别研究
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作者 陈研 冯露露 +1 位作者 黄荣 齐伟辰 《世界科学技术-中医药现代化》 CSCD 北大核心 2024年第2期490-501,共12页
目的开发出能同时鉴别北苍术和关苍术的分子标记方法,并探究不同种质资源苍术的遗传进化关系。方法对不同地区北苍术Atractylodes chinensis(Bunge)Koidz及关苍术A.japonica Koidz.ex Kitam基因组DNA的差异片段进行测序,结合SRAP、ISSR... 目的开发出能同时鉴别北苍术和关苍术的分子标记方法,并探究不同种质资源苍术的遗传进化关系。方法对不同地区北苍术Atractylodes chinensis(Bunge)Koidz及关苍术A.japonica Koidz.ex Kitam基因组DNA的差异片段进行测序,结合SRAP、ISSR、DAMD分子标记方法,优化PCR反应体系,筛选并转换成特异性标记,同时,采用条形码方法分析种间序列差异。结果通过SRAP、ISSR、DAMD三种分子标记方法的PCR扩增,共筛选出198对能稳定扩增且重现性好的引物,转换出7对能稳定、快速鉴别北苍术和关苍术的SCAR引物。条形码方法检测出北苍术ITS2序列长度为454 bp,关苍术ITS2序列长度为453 bp,与其他苍术属植物之间遗传距离较远。NJ树结果显示,北苍术、关苍术及其他苍术属植物均各自聚为一支,表现出良好的单系性。依据ITS2二级结构,4种苍术属植物在螺旋区的茎环数目、大小、位置均有明显差异,可以直观地进行区分。结论所开发的特异性SCAR标记为苍术属植物优良品种的筛选提供了新方法,DNA条形码能稳定、准确鉴别北苍术。 展开更多
关键词 北苍术 关苍术 Internal transcribed spacer 2(ITS2) Sequence-related amplified polymorphism(SRAP) Inter-simple sequence repeat(ISSR) Direct amplification of minisatellite region DNA(DAMD) Sequence characterized amplified regions(scar)
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阿拉尔棉田烟粉虱捕食性天敌的SCAR分子标记检测
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作者 赵宇 杨清川 +3 位作者 梁静 孙雨 杨晓东 杨明禄 《现代农业科技》 2024年第13期161-164,172,共5页
为了明确阿拉尔垦区棉田烟粉虱种群隐种以及烟粉虱捕食性天敌种类,采集阿拉尔十二团棉田中的烟粉虱及烟粉虱捕食性天敌,利用B-F/B-R、Q-F/Q-R特异性引物检测烟粉虱隐种类型,利用烟粉虱TB-F94585/TB-R 94585特异性引物检测捕食性天敌肠... 为了明确阿拉尔垦区棉田烟粉虱种群隐种以及烟粉虱捕食性天敌种类,采集阿拉尔十二团棉田中的烟粉虱及烟粉虱捕食性天敌,利用B-F/B-R、Q-F/Q-R特异性引物检测烟粉虱隐种类型,利用烟粉虱TB-F94585/TB-R 94585特异性引物检测捕食性天敌肠道内是否存在烟粉虱DNA。结果表明:新疆阿拉尔垦区烟粉虱为MED隐种,初步发现烟粉虱的捕食天敌共有4目5科,包括多异瓢虫成虫、多异瓢虫幼虫、东亚小花蝽、中华草蛉幼虫、黄褐新园蛛、灌木新园蛛、直伸肖蛸。利用SCAR分子标记技术可以快速检测阿拉尔垦区烟粉虱捕食性天敌种类。 展开更多
关键词 烟粉虱 scar分子标记 捕食性天敌 棉田 新疆阿拉尔
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Astrocytes, reactive astrogliosis, and glial scar formation in traumatic brain injury
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作者 María Belén Cieri Alberto Javier Ramos 《Neural Regeneration Research》 SCIE CAS 2025年第4期973-989,共17页
Traumatic brain injury is a global health crisis,causing significant death and disability worldwide.Neuroinflammation that follows traumatic brain injury has serious consequences for neuronal survival and cognitive im... Traumatic brain injury is a global health crisis,causing significant death and disability worldwide.Neuroinflammation that follows traumatic brain injury has serious consequences for neuronal survival and cognitive impairments,with astrocytes involved in this response.Following traumatic brain injury,astrocytes rapidly become reactive,and astrogliosis propagates from the injury core to distant brain regions.Homeostatic astroglial proteins are downregulated near the traumatic brain injury core,while pro-inflammatory astroglial genes are overexpressed.This altered gene expression is considered a pathological remodeling of astrocytes that produces serious consequences for neuronal survival and cognitive recovery.In addition,glial scar formed by reactive astrocytes is initially necessary to limit immune cell infiltration,but in the long term impedes axonal reconnection and functional recovery.Current therapeutic strategies for traumatic brain injury are focused on preventing acute complications.Statins,cannabinoids,progesterone,beta-blockers,and cerebrolysin demonstrate neuroprotective benefits but most of them have not been studied in the context of astrocytes.In this review,we discuss the cell signaling pathways activated in reactive astrocytes following traumatic brain injury and we discuss some of the potential new strategies aimed to modulate astroglial responses in traumatic brain injury,especially using cell-targeted strategies with miRNAs or lncRNA,viral vectors,and repurposed drugs. 展开更多
关键词 ASTROCYTE glial scar innate immunity NEUROINFLAMMATION stab injury Toll-like receptors
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Endoscopic treatment of scarred polyps with a non-thermal device(Endorotor):A review of the literature
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作者 Mariam Zaghloul Hameed Rehman +2 位作者 Stefano Sansone Konstantinos Argyriou Adolfo Parra-Blanco 《World Journal of Gastroenterology》 SCIE CAS 2024年第12期1706-1713,共8页
Endoscopic resection(ER)of colorectal polyps has become a daily practice in most endoscopic units providing a colorectal cancer screening program and requires the availability of local experts and high-end endoscopic ... Endoscopic resection(ER)of colorectal polyps has become a daily practice in most endoscopic units providing a colorectal cancer screening program and requires the availability of local experts and high-end endoscopic devices.ER procedures have evolved over the past few years from endoscopic mucosal resection(EMR)to more advanced techniques,such as endoscopic submucosal dissection and endo-scopic full-thickness resection.Complete resection and disease eradication are the ultimate goals of ER-based techniques,and novel devices have been developed to achieve these goals.The EndoRotor®Endoscopic Powered Resection System(Interscope Medical,Inc.,Northbridge,Massachusetts,United States)is one such device.The EndoRotor is a powered resection tool for the removal of alimentary tract mucosa,including post-EMR persistent lesions with scarring,and has both CE Mark and FDA clearance.This review covers available published evidence documenting the usefulness of EndoRotor for the management of recurrent colorectal polyps. 展开更多
关键词 EndoRotor scarred polyps Recurrent polyps Colorectal cancer Colorectal polyps
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Exploring the potential mechanism of WuFuYin against hypertrophic scar using network pharmacology and molecular docking
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作者 Shu-Yang Zhang Song-Xue Guo +4 位作者 Lei-Lei Chen Jia-Yan Zhu Ming-Sheng Hou Jia-Ke Lu Xue-Xiang Shen 《World Journal of Clinical Cases》 SCIE 2024年第18期3505-3514,共10页
BACKGROUND Hypertrophic scar(HTS)is dermal fibroproliferative disorder,which may cause physiological and psychological problems.Currently,the potential mechanism of WuFuYin(WFY)in the treatment of HTS remained to be e... BACKGROUND Hypertrophic scar(HTS)is dermal fibroproliferative disorder,which may cause physiological and psychological problems.Currently,the potential mechanism of WuFuYin(WFY)in the treatment of HTS remained to be elucidated.AIM To explore the potential mechanism of WFY in treating HTS.METHODS Active components and corresponding targets were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.HTSrelated genes were obtained from the GeneCards,DisGeNET,and National Center for Biotechnology Information.The function of targets was analyzed by performing Gene Ontology and Kyoto Encyclopaedia of Genes and Genome(KEGG)enrichment analysis.A protein+IBM-protein interaction(PPI)network was developed using STRING database and Cytoscape.To confirm the high affinity between compounds and targets,molecular docking was performed.RESULTS A total of 65 core genes,which were both related to compounds and HTS,were selected from multiple databases.PPI analysis showed that CKD2,ABCC1,MMP2,MMP9,glycogen synthase kinase 3 beta(GSK3B),PRARG,MMP3,and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma(PIK3CG)were the hub targets and MOL004941,MOL004935,MOL004866,MOL004993,and MOL004989 were the key compounds of WFY against HTS.The results of KEGG enrichment analysis demonstrated that the function of most genes were enriched in the PI3K-Akt pathway.Moreover,by performing molecular docking,we confirmed that GSK3B and 8-prenylated eriodictyol shared the highest affinity.CONCLUSION The current findings showed that the GSK3B and cyclin dependent kinase 2 were the potential targets and MOL004941,MOL004989,and MOL004993 were the main compounds of WFY in HTS treatment. 展开更多
关键词 WuFuYin Hypertrophic scar Network pharmacology Molecular docking Enrichment analysis
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The global Antarctic Research event,SCAR 2024
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《Advances in Polar Science》 CSCD 2024年第1期141-141,共1页
We are pleased to announce that“The 11th SCAR Open Science Conference”will take place in Pucón,Chile from 19-23 August 2024,hosted by the Chilean Antarctic Institute.Detailed information at https://www.scar2024... We are pleased to announce that“The 11th SCAR Open Science Conference”will take place in Pucón,Chile from 19-23 August 2024,hosted by the Chilean Antarctic Institute.Detailed information at https://www.scar2024.org/.The 11th SCAR Open Science Conference theme“Antarctic Science:Crossroads for a New Hope”,recognizes the importance of Antarctica as a unique and fragile ecosystem.With increasing concerns about climate change and its impact on the polar regions,this theme aims to highlight the significance of Antarctic research in shaping our understanding of global environmental challenges.By fostering collaboration and knowledge exchange,the SCAR Open Science conference hopes to inspire new solutions and a renewed sense of hope for the future of our planet. 展开更多
关键词 ANTARCTIC GLOBAL scar
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Tamanu Oil in Acne Management: Potential Anti-Inflammatory and Wound-Healing Properties for Scar Reduction
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作者 Rachel Marciano Alexandra Loperfito +5 位作者 Maddie Moll Allison Liu Yuna Huh Nicole Werpachowski Kelly Frasier Julia Vinagolu-Baur 《Journal of Biomedical Science and Engineering》 2024年第10期185-193,共9页
Tamanu oil, derived from the nuts of Calophyllum inophyllum, has gained increasing attention for its potential in acne management due to its purported anti-inflammatory and wound-healing properties. This analysis eval... Tamanu oil, derived from the nuts of Calophyllum inophyllum, has gained increasing attention for its potential in acne management due to its purported anti-inflammatory and wound-healing properties. This analysis evaluates the efficacy of tamanu oil in acne treatment with a specific focus on its impact on inflammation and scar reduction. The novelty of this research lies in its comprehensive analysis of tamanu oil’s dual mechanism of action: reducing acne-related inflammation and promoting the healing of acne scars. Clinical trials and laboratory analyses were conducted to assess the oil’s effectiveness in diminishing erythema, swelling, and post-acne scarring compared to conventional treatments. Preliminary findings demonstrate that tamanu oil significantly reduces inflammation and accelerates wound healing, potentially offering a promising adjunct or alternative to standard acne therapies. Future research should aim to optimize formulation and application protocols, long-term effects, and comparative therapeutic efficacy with other anti-inflammatory agents. Tamanu oil offers a novel and effective approach to acne management, with potential advantages that go beyond inflammation reduction to include enhanced scar reduction, making it a subject that warrants further investigation. 展开更多
关键词 Tamanu Oil Calophyllum inophyllum Acne Management Anti-Inflammatory Properties Wound Healing Acne scars Erythema Reduction Inflammation Reduction scar Reduction Alternative Acne Therapies Natural Acne Treatments Dermatological Applications of Tamanu Oil
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Vaginal Cesarean Section, an Alternative to High-Risk Trigger on Scarred Uterus
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作者 Famakan Kane Mahamadou Keita +3 位作者 Yacouba Sylla Soumaila Diallo Diassana Mahamadou Traore Tidiane 《Open Journal of Obstetrics and Gynecology》 2024年第7期979-982,共4页
The objective is to report a clinical case of vaginal cesarean section performed to expel a dead fetus in scarred uterus. For this indication, vaginal hysterectomy constitutes an alternative to vaginal expulsion with ... The objective is to report a clinical case of vaginal cesarean section performed to expel a dead fetus in scarred uterus. For this indication, vaginal hysterectomy constitutes an alternative to vaginal expulsion with a high risk of uterine rupture and to classic abdominal cesarean section with risk of significant surgical trauma, particularly adhesions. However, this surgical technique, described since the 19th century, remains unknown to many practitioners and few publications exist on the subject throughout the world. Considered obsolete by some practitioners, it retains all its advantages in the practice of modern obstetrics. We report this case of expulsion of fetal death on a tri-scarred uterus performed by vaginal cesarean section at the Health District Reference Health Center (District Hospital) of Commune I in Bamako, Mali in a 37-year-old patient with a pregnancy of 27 weeks of amenorrhea. 展开更多
关键词 Vaginal Cesarean Section Birth on scarred Uterus In Utero Fetal Death
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The Use of Triancinolone for the Treatment of Keloid Scars
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作者 José Humberto Cardoso Resende Luís Otávio Torres +6 位作者 Anderson Alves Brandão Delma dos Santos Assis Mercadante Jessyka Oliveira Barbosa Batista William Felix de Oliveira Pacheco Elisangelo Aparecido Costa Da Silva Douglas Henrique Farias de Oliveira Nébia Maria Almeida de Figueiredo 《Journal of Cosmetics, Dermatological Sciences and Applications》 2024年第3期270-275,共6页
Scars, when in good evolution, result in a smooth, thin and discreet tissue. Keloid scars, however, are a type of abnormal and exacerbated repair response to tissue injury, whether in surgical interventions or in vari... Scars, when in good evolution, result in a smooth, thin and discreet tissue. Keloid scars, however, are a type of abnormal and exacerbated repair response to tissue injury, whether in surgical interventions or in various injuries, which present in a prominent and gross way. In this context, there is an excess of collagen deposition in the tissue repair process, which can lead to the formation of keloids. The diagnosis of the condition presented is made by the medical professional or by the patient himself after the surgical intervention or skin injury. Under this analysis, protruding, rough and bad-looking scars are identified. In addition, we highlight the existence of keloids similar to large tumors, described as Jorge Lobo disease. The treatment encompasses massages, compressions, corticosteroids, chemotherapy, collagenase and cryotherapy. At first, we used corticosteroid-based massages, and then we started using compressive dressings until we started intrakeloid infiltrations with injectable triamcinolone. Triamcinolone 10 mg injectable—1/10—in 0.9% saline, with syringe and fixed needle 0.3 mm × 8 mm, intralesional infiltrate, in this context, proved to be effective for its treatment when applied sequentially and linearly. In cases where the medication was applied, there was an improvement after 21 days of application and a definitive improvement 2 months after the injury. In comparison, on the other hand, patients who were not subjected to the application of the medication may improve after 4 months of the injury or worsen compared to the initial case. We have come to the conclusion that this procedure may be one of the chosen ones for the treatment of keloid scars, being one of the most recommended for cases of keloid already installed. 展开更多
关键词 KELOID scar HYPERTROPHY COLLAGEN AFFECTION
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通过改良RAPD技术获得黑毛石斛、蜂腰石斛和石仙桃的3条SCAR新标记
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作者 梅志强 郭侃 +2 位作者 魏春莉 郑美玲 傅俊江 《西南医科大学学报》 2024年第1期29-33,共5页
目的寻找快速区分黑毛石斛、蜂腰石斛和石仙桃的序列特征扩增区域(sequence characterized amplified regions,SCAR)新标记。方法在改良的随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)的基础上对10个物种进行基因组DNA随... 目的寻找快速区分黑毛石斛、蜂腰石斛和石仙桃的序列特征扩增区域(sequence characterized amplified regions,SCAR)新标记。方法在改良的随机扩增多态性DNA(random amplified polymorphic DNA,RAPD)的基础上对10个物种进行基因组DNA随机扩增、分子克隆和测序,分别设计了可鉴别3个物种的SCAR标记。结果成功筛选出3个SCAR标记,其相应的特异性标记分别为N2-12、A2-17和A6-33;其中SCAR标记N2-12在黑毛种属中扩增出约750 bp的特异性条带,SCAR标记A2-17在蜂腰石斛中扩增出约750 bp的特异性条带,SCAR标记A6-33在石仙桃种属中扩增出约600 bp的特异性条带。结论N2-12、A2-17和A6-33这3对特异性SCAR新标记能快速、准确的区分出黑毛石斛、蜂腰石斛和石仙桃这3个物种,为今后进行该中药道地药材样品的鉴定提供了实验依据。 展开更多
关键词 黑毛石斛 蜂腰石斛 石仙桃 序列特征扩增区域标记 鉴定
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Microglial depletion impairs glial scar formation and aggravates inflammation partly by inhibiting STAT3 phosphorylation in astrocytes after spinal cord injury 被引量:9
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作者 Zhi-Lai Zhou Huan Xie +4 位作者 Xiao-Bo Tian Hua-Li Xu Wei Li Shun Yao Hui Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第6期1325-1331,共7页
Astrocytes and microglia play an orchestrated role following spinal cord injury;however,the molecular mechanisms through which microglia regulate astrocytes after spinal cord injury are not yet fully understood.Herein... Astrocytes and microglia play an orchestrated role following spinal cord injury;however,the molecular mechanisms through which microglia regulate astrocytes after spinal cord injury are not yet fully understood.Herein,microglia were pharmacologically depleted and the effects on the astrocytic response were examined.We further explored the potential mechanisms involving the signal transducers and activators of transcription 3(STAT3)pathway.For in vivo experiments,we constructed a contusion spinal cord injury model in C57BL/6 mice.To deplete microglia,all mice were treated with colony-stimulating factor 1 receptor inhibitor PLX3397,starting 2 weeks prior to surgery until they were sacrificed.Cell proliferation was examined by 5-ethynyl-2-deoxyuridine(EdU)and three pivotal inflammatory cytokines were detected by a specific Bio-Plex Pro^(TM) Reagent Kit.Locomotor function,neuroinflammation,astrocyte activation and phosphorylated STAT3(pSTAT3,a maker of activation of STAT3 signaling)levels were determined.For in vitro experiments,a microglia and astrocyte coculture system was established,and the small molecule STA21,which blocks STAT3 activation,was applied to investigate whether STAT3 signaling is involved in mediating astrocyte proliferation induced by microglia.PLX3397 administration disrupted glial scar formation,increased inflammatory spillover,induced diffuse tissue damage and impaired functional recovery after spinal cord injury.Microglial depletion markedly reduced EdU+proliferating cells,especially proliferating astrocytes at 7 days after spinal cord injury.RNA sequencing analysis showed that the JAK/STAT3 pathway was downregulated in mice treated with PLX3397.Double immunofluorescence staining confirmed that PLX3397 significantly decreased STAT3 expression in astrocytes.Importantly,in vitro coculture of astrocytes and microglia showed that microglia-induced astrocyte proliferation was abolished by STA21 administration.These findings suggest that microglial depletion impaired astrocyte proliferation and astrocytic scar formation,and induced inflammatory diffusion partly by inhibiting STAT3 phosphorylation in astrocytes following spinal cord injury. 展开更多
关键词 ASTROCYTES COCULTURE colony-stimulating factor 1 receptor inhibitor EdU glia scar inflammatory response microglia PHOSPHORYLATION proliferation spinal cord injury STAT3
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M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats 被引量:2
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作者 Jia-Gui Huang Jiang-Xia Ren +9 位作者 Yue Chen Ming-Fen Tian Li Zhou Jun Wen Xiao-Song Song You-Lin Wu Qing-Huan Yang Pei-Ran Jiang Jia-Ni Wang Qin Yang 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第10期2208-2218,共11页
In the central nervous system, the formation of fibrotic scar after injury inhibits axon regeneration and promotes repair. However, the mechanism underlying fibrotic scar formation and regulation remains poorly unders... In the central nervous system, the formation of fibrotic scar after injury inhibits axon regeneration and promotes repair. However, the mechanism underlying fibrotic scar formation and regulation remains poorly understood. M2 macrophages regulate fibrotic scar formation after injury to the heart, lung, kidney, and central nervous system. However, it remains to be clarified whether and how M2 macrophages regulate fibrotic scar formation after cerebral ischemia injury. In this study, we found that, in a rat model of cerebral ischemia induced by middle cerebral artery occlusion/reperfusion, fibrosis and macrophage infiltration were apparent in the ischemic core in the early stage of injury(within 14 days of injury). The number of infiltrated macrophages was positively correlated with fibronectin expression. Depletion of circulating monocyte-derived macrophages attenuated fibrotic scar formation. Interleukin 4(IL4) expression was strongly enhanced in the ischemic cerebral tissues, and IL4-induced M2 macrophage polarization promoted fibrotic scar formation in the ischemic core. In addition, macrophage-conditioned medium directly promoted fibroblast proliferation and the production of extracellular matrix proteins in vitro. Further pharmacological and genetic analyses showed that sonic hedgehog secreted by M2 macrophages promoted fibrogenesis in vitro and in vivo, and that this process was mediated by secretion of the key fibrosis-associated regulatory proteins transforming growth factor beta 1 and matrix metalloproteinase 9. Furthermore, IL4-afforded functional restoration on angiogenesis, cell apoptosis, and infarct volume in the ischemic core of cerebral ischemia rats were markedly impaired by treatment with an sonic hedgehog signaling inhibitor, paralleling the extent of fibrosis. Taken together, our findings show that IL4/sonic hedgehog/transforming growth factor beta 1 signaling targeting macrophages regulates the formation of fibrotic scar and is a potential therapeutic target for ischemic stroke. 展开更多
关键词 central nervous system extracellular matrix FIBRONECTIN fibrotic scar macrophage interleukin 4 ischemic cerebral injury neurological function Sonic hedgehog transforming growth factorβ1
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Temporal dynamics of microglia-astrocyte interaction in neuroprotective glial scar formation after intracerebral hemorrhage 被引量:1
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作者 Jingwei Zheng Haijian Wu +11 位作者 Xiaoyu Wang Guoqiang Zhang Jia'nan Lu Weilin Xu Shenbin Xu Yuanjian Fang Anke Zhang Anwen Shao Sheng Chen Zhen Zhao Jianmin Zhang Jun Yu 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第8期862-879,共18页
The role of glial scar after intracerebral hemorrhage(ICH)remains unclear.This study aimed to investigate whether microglia-astrocyte interaction affects glial scar formation and explore the specific function of glial... The role of glial scar after intracerebral hemorrhage(ICH)remains unclear.This study aimed to investigate whether microglia-astrocyte interaction affects glial scar formation and explore the specific function of glial scar.We used a pharmacologic approach to induce microglial depletion during different ICH stages and examine how ablating microglia affects astrocytic scar formation.Spatial transcriptomics(ST)analysis was performed to explore the potential ligand-receptor pair in the modulation of microglia-astrocyte interaction and to verify the functional changes of astrocytic scars at different periods.During the early stage,sustained microglial depletion induced disorganized astrocytic scar,enhanced neutrophil infiltration,and impaired tissue repair.ST analysis indicated that microglia-derived insulin like growth factor 1(IGF1)modulated astrocytic scar formation via mechanistic target of rapamycin(mTOR)signaling activation.Moreover,repopulating microglia(RM)more strongly activated mTOR signaling,facilitating a more protective scar formation.The combination of IGF1 and osteopontin(OPN)was necessary and sufficient for RM function,rather than IGF1 or OPN alone.At the chronic stage of ICH,the overall net effect of astrocytic scar changed from protective to destructive and delayed microglial depletion could partly reverse this.The vital insight gleaned from our data is that sustained microglial depletion may not be a reasonable treatment strategy for early-stage ICH.Inversely,early-stage IGF1/OPN treatment combined with late-stage PLX3397 treatment is a promising therapeutic strategy.This prompts us to consider the complex temporal dynamics and overall net effect of microglia and astrocytes,and develop elaborate treatment strategies at precise time points after ICH. 展开更多
关键词 MICROGLIA ASTROCYTES Glial scar Intracerebral hemorrhage
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Emergency internal iliac artery temporary occlusion after massive hemorrhage during surgery of cesarean scar pregnancy:A case report 被引量:1
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作者 Ji-Ping Xie Lin-Lin Chen +3 位作者 Wen Lv Wu Li Hui Fang Guang Zhu 《World Journal of Clinical Cases》 SCIE 2023年第17期4065-4071,共7页
BACKGROUND Cesarean scar pregnancy(CSP)is rare but may result in uterine rupture during pregnancy or massive hemorrhage during abortion procedures.Awareness of this condition is increasing,and most patients with CSP a... BACKGROUND Cesarean scar pregnancy(CSP)is rare but may result in uterine rupture during pregnancy or massive hemorrhage during abortion procedures.Awareness of this condition is increasing,and most patients with CSP are now diagnosed early and can be managed safely.However,some atypical patients are misdiagnosed,and their surgical risks are underestimated,increasing the risk of fatal hemorrhage.CASE SUMMARY A 27-year-old Asian woman visited our institution because of abnormal pregnancy,and she was diagnosed with a hydatidiform mole through transvaginal ultrasound(TVS).Under hysteroscopy,a large amount of placental tissue was found in the scar of the lower uterine segment,and a sudden massive hemorrhage occurred during the removal process.The bilateral internal iliac arteries were temporarily blocked under laparoscopy,and scar resection and repair were rapidly performed.She was discharged in good condition 5 d after the operation.CONCLUSION Although TVS is widely used in the diagnosis of CSP,delays in the diagnosis of atypical CSP remain.Surgical treatment following internal iliac artery temporary occlusion may be an appropriate management method for unanticipated massive hemorrhage during CSP surgery. 展开更多
关键词 Internal iliac artery temporary occlusion Cesarean scar pregnancy Uterine artery embolization MISDIAGNOSIS HYSTEROSCOPY LAPAROSCOPY Case report
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Can we suppress excessive post-surgical scar formation:A case report 被引量:1
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作者 Mir Sadat-Ali Sulaiman A Al-Mousa +2 位作者 Khalid Waleed Al-Tabash Mohamed M Abotaleb Fawaz M Al-Anii 《World Journal of Orthopedics》 2023年第3期166-170,共5页
BACKGROUND Hypertrophic scars(HSs)formation is a complication that occurs after wounds heal with secondary intention and sometimes after clean surgical incisions.Many treatments are in vogue now with varying successes... BACKGROUND Hypertrophic scars(HSs)formation is a complication that occurs after wounds heal with secondary intention and sometimes after clean surgical incisions.Many treatments are in vogue now with varying successes.Although the mechanism or mechanisms that cause a HS to form are not clearly understood,one thing that is clear is that once scar tissue matures,any intervention will not be successful.In this paper,we report on a case where a patient who was known to develop HS was treated with a new combination of ingredients(Phyto-chemicals+Silicone JUMI)to suppress HS formation.CASE SUMMARY A 68-year-old female of African descent presented a severe HS post total knee replacement(TKR),which the patient describes as itchy and painful.Due to complications caused by the scar,she was apprehensive about undergoing TKR on her other knee.However,after the TKR of the contralateral side post-removal of skin clips,JUMI anti-scar cream(JASC)was used to suppress excessive scar formation.CONCLUSION JASC appears potent and efficacious at suppressing excessive scar formation.We believe that this warrants further studies on larger patient groups and on different surgical sites. 展开更多
关键词 Hypertrophic scars Photo-chemicals JUMI KELOID Case report©The Author(s)2023.Published by Baishideng Publishing Group Inc.All rights reserved.
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基于sCARS-PSO-SVM的土壤硒含量高光谱定量反演
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作者 谢鹏 王正海 +3 位作者 肖蓓 曹海玲 黄意 苏文林 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2023年第11期3599-3606,共8页
硒(Se)是人体必需的微量元素之一。人主要通过食用农产品来获取硒,而农产品中的硒主要来自土壤。因此,研究土壤中硒的含量和分布,对人体健康和农作物生产具有重要的意义。高光谱遥感技术的发展,使得高效、低成本、大范围估测土壤中硒的... 硒(Se)是人体必需的微量元素之一。人主要通过食用农产品来获取硒,而农产品中的硒主要来自土壤。因此,研究土壤中硒的含量和分布,对人体健康和农作物生产具有重要的意义。高光谱遥感技术的发展,使得高效、低成本、大范围估测土壤中硒的含量和分布成为可能。但是,土壤中硒的含量对光谱的敏感性较弱,严重影响了高光谱硒含量定量反演精度。该研究以广东连州地区富硒土壤为研究对象,系统采集研究区土壤样品50份,分析土壤样本硒含量,同步采集土壤反射光谱数据;利用Savitzky-Golay卷积平滑算法、多元散射校正(MSC)、对数一阶微分(lg(R)-FD)、标准正态变量校正(SNV)、多元散射校正一阶微分(MSC-FD)对原始光谱进行增强处理;应用稳定竞争自适应重加权采样(sCARS)算法结合皮尔逊相关性分析(PCC)进行特征波段选择;对比分析偏最小二乘(PLS)、支持向量机(SVM)和粒子群优化支持向量(PSO-SVM)模型土壤硒含量高光谱定量反演效果。结果表明:将sCARS算法应用于光谱增强后的回归模型,并结合皮尔逊相关性(PCC)选择与土壤硒含量敏感性较大的特征波段,不仅可以降低土壤硒含量高光谱预测模型复杂度并有效避免大量有用信息的损失,还能提高高光谱回归模型的反演效率;对比不同回归模型训练集和预测集的决定系数R^(2)和均方根误差RMSE,发现支持向量(SVM)模型比偏最小二乘(PLSR)模型预测效果更好,模型稳定性更高,且非线性模型更适用于土壤硒含量的预测;通过粒子群(PSO)算法优化SVM的核函数和正则化参数,SVM模型的反演精度和稳定性都有所提升;MSC-PSO-SVM模型(R^(2)=0.53、RMSE=0.34)和MSC-FD模型(R^(2)=0.50、RMSE=0.04)预测效果较为突出。综上所述:利用sCARS结合PSO-SVM算法建立土壤硒含量的高光谱定量反演模型,能够为土壤硒含量的高光谱大面积估测提供新的途径。 展开更多
关键词 高光谱 土壤Se含量 scarS PSO-SVM
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不同类型剖宫产瘢痕妊娠的病例特点与诊治分析 被引量:2
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作者 王超 侯征 +2 位作者 李华军 李蓉 乔杰 《中国全科医学》 北大核心 2024年第12期1475-1479,1486,共6页
背景现行剖宫产瘢痕妊娠(CSP)分型依据仅参照超声影像特点,目前尚缺乏该分型标准下不同类型CSP病例临床特点的分析总结。目的探讨不同类型CSP的病例特点及诊治差异。方法纳入北京大学第三医院妇产科2014年7月—2022年6月收治的CSP患者共... 背景现行剖宫产瘢痕妊娠(CSP)分型依据仅参照超声影像特点,目前尚缺乏该分型标准下不同类型CSP病例临床特点的分析总结。目的探讨不同类型CSP的病例特点及诊治差异。方法纳入北京大学第三医院妇产科2014年7月—2022年6月收治的CSP患者共862例为研究对象,根据超声分型标准分为Ⅰ型、Ⅱ型、Ⅲ型组,并对其临床特点及诊治指标进行回顾性分析。结果本研究CSP患者中Ⅰ型组占36.5%(315/862),Ⅱ型组占53.1%(458/862),Ⅲ型组占10.3%(89/862)。3组患者的年龄、孕产史、既往宫腔手术史比例比较,差异均无统计学意义(P>0.05)。CSP患者中腹痛发生率为24.2%(209/862),阴道出血发生率为65.0%(560/862)。3组CSP患者腹痛及阴道出血发生率比较,差异均无统计学意义(P=0.261、0.062)。Ⅲ型组患者诊断时停经时间为55(46,64)d,妊娠物中位径线长29.6(19.1,43.3)mm,术前血β-人绒毛膜促性腺激素(β-HCG)水平为60673(17164,122203)mU/mL,需辅助药物杀胚治疗、腹腔镜监视下手术、子宫动脉阻断率分别为27.0%(24/89)、33.7%(30/89)、32.6%(29/89),手术时长101(67,125)min,住院时间4(3,7)d,治疗花费11933.7(8760.7,15250.6)元,术后24 h累计出血量、出血≥200 mL发生率及输血率分别为83(33,178)mL、24.7%(22/89)、7.9%(7/89),均高于其他两组(P<0.001)。所有患者持续性CSP发生率为3.1%(27/862),3组持续性CSP发生率比较,差异无统计学意义(χ^(2)=3.353,P=0.187)。结论不同类型CSP患者的年龄、孕产史、既往宫腔手术史及腹痛、阴道出血等临床特点无明显差异。Ⅰ型和Ⅱ型患者治疗侵入性较小,Ⅲ型患者的医疗资源消耗较多,对多学科团队及个体化管理有较高要求。不同类型患者经规范管理,其治疗预后均较理想。 展开更多
关键词 剖宫产术 瘢痕妊娠 体征和症状 治疗 预后
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Effectiveness of conjunctival bleb scarring by knockdown of heat shock protein 47 in rat model
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作者 Wei-Wei Wang Hai-Yan Li Huan-Huan Yan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第10期1589-1594,共6页
AIM:To evaluate the effectiveness of knock-down of heat shock protein 47(HSP47)on conjunctival bleb scarring in a rat model and its possible mechanism.METHODS:Male Sprague–Dawley rats were used for glaucoma filtratio... AIM:To evaluate the effectiveness of knock-down of heat shock protein 47(HSP47)on conjunctival bleb scarring in a rat model and its possible mechanism.METHODS:Male Sprague–Dawley rats were used for glaucoma filtration surgery(GFS)and were treated with either phosphate buffered solution,shControl,mitomycin C,or sh-HSP47 using a microsyringe immediately after GFS.The morphology of filtering blebs was observed postoperatively.The levels of HSP47 were analyzed at 2,5,8,and 11d after GFS via real‑time quantitative polymerase chain reaction(PCR)and Western blot.The silencing effect of HSP47,the expression of collagen I and III,and the potential signaling pathways of HSP47 during scarification were explored 11d post GFS.The protein levels of transforming growth factor-β1(TGF-β1),phospho-Smad2(pSmad2),phospho-Smad3(p-Smad3),and phospho-p38(p-p38)were also analyzed using Western blot.RESULTS:Sh-HSP47 treatment significantly prolonged the functional filtration bleb retention.The levels of HSP47 were increased significantly at 5,8,and 11d postoperatively compared to the control group(P<0.05,P<0.01,and P<0.001).The levels of HSP47 protein at day 11 postoperatively were significantly down-regulated after HSP47 silencing using sh-HSP47 adenovirus transfection(P<0.01).Expression levels of collagen I and III within the blebs were significantly reduced in the absence of HSP47(P<0.01).Moreover,the protein levels of TGF-β1,p-Smad2/3,and p-p38 were dramatically inhibited after treatment with sh-HSP47(P<0.01).CONCLUSION:The inhibitory effects of HSP47 knockdown on scarring after GFS have the potential to be an efficacious therapeutic option for the treatment of conjunctival bleb scarring. 展开更多
关键词 heat shock protein 47 filtration surgery conjunctival bleb scar transforming growth factor-β1
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