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Development, Validation and Application of a Spectrofluorimetric Method for the Quantification of Nevirapine in Pharmaceutical Formulations Tablets and Suspensions
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作者 Lucien Murhula Namegabe Aladin Ombeni Mahano Serigne Omar Sarr 《American Journal of Analytical Chemistry》 2022年第6期206-227,共22页
The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirap... The development of the spectrofluorimetric method can be considered a promising alternative that is relatively less expensive and sufficiently reliable. In the current literature, no method for the analysis of nevirapine by spectro-fluorimetric has been reported. The proposed method is based on the transformation of naturally non-fluorescent nevirapine into a fluorescent derivative after chemical synthesis. Maximum excitation and emission wavelengths are 290 nm and 357 nm respectively. The analytical performance of the method demonstrates linearity in the concentration range 1.5 × 10<sup>-2</sup> and 13.5 × 10<sup>-2</sup> μg/mL with a correlation coefficient (r) greater than 0.999. The detection (LOD) and quantification (LOQ) limits found are 1.97 × 10<sup>-3</sup> μg/mL and 5.48 × 10<sup>-3</sup> μg/mL respectively. Recovery is achieved with 99.9% and 100.3% trueness, intra-day precision with a coefficient of variation of repeatability (CVr) of 0.99% and inter-day precision with a coefficient of variation of precision (CVR) of 1.7%. The method has been successfully applied in the analysis of 10 batches of nevirapine tablets and suspensions. 展开更多
关键词 spectrofluorimetric method Validation Nevirapine Sodium Hypochlorite
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Sensitive Spectrofluorimetric Method of Analysis for Gabapentin in Pure and Pharmaceutical Preparations
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作者 Ulu, Sevgi Tatar Kel, Elif 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2011年第3期562-566,共5页
Highly sensitive spectrofluorimetric method was developed and validated for the determination of gabapentin in pure and pharmaceutical preparations. The method was based on nucleophilic substitution reaction of gabape... Highly sensitive spectrofluorimetric method was developed and validated for the determination of gabapentin in pure and pharmaceutical preparations. The method was based on nucleophilic substitution reaction of gabapentin with 4-fluoro-7-nitrobenzofurazan (NBD-F) in an alkaline medium (pH 9.5) to form a highly fluorescent derivative that was measured at 521 nm after excitation at 458 rim. The factors affecting the reaction was carefully studied and optimized. The method was successfully validated for linearity, limit of detection, limit of quantification, accuracy, precision, robustness and specificity. Under the optimized conditions, linear relationship with good correlation coefficient (0.9998) was found between the relative fluorescence intensity and gabapentin concentration in the range of 10--100 ng·mL^-1. The limit of detection and limit of quantification were 0.43 and 1.30 ng·mL ^-1, respectively. The proposed method was successfully applied to the determination of gabapentin in its pharmaceutical capsules. The results obtained by the proposed method were comparable with those obtained by the official method. Statistical comparison by t- and F- tests revealed that there was no significant difference between the results of the two methods with respect to mean values and standard deviations at the 95% confidence level. 展开更多
关键词 GABAPENTIN NBD-F spectrofluorimetric method DERIVATIZATION DETERMINATION
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Fluorimetric study of the interaction between ATP and ciprofloxacin-Y^(3+) complex and its application 被引量:2
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作者 Shailaja R.Patil Umesh S.Mote +2 位作者 Shivajirao R.Patil Sang-Hak Lee Govind B.Kolekar 《Journal of Rare Earths》 SCIE EI CAS CSCD 2010年第3期329-332,共4页
A simple spectrofluorimetric method was developed for determination of trace amount of adenosine disodium triphosphate(ATP).Under the optimum conditions,we studied the interaction between CIP-Y3+-ATP complex by using ... A simple spectrofluorimetric method was developed for determination of trace amount of adenosine disodium triphosphate(ATP).Under the optimum conditions,we studied the interaction between CIP-Y3+-ATP complex by using absorption and fluorescence spectra.It was observed that ATP remarkably enhanced the fluorescence intensity of the CIP-Y3+ complex at λem=415 nm and the enhanced fluorescence intensity of Y3+ ion remained proportional to the concentration of ATP.The developed method was successfully applied for... 展开更多
关键词 adenosine disodium triphosphate(ATP) ciprofloxacin(CIP) yttrium(Y3+) spectrofluorimetric method rare earths
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