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COPD患者血清TRAIL表达水平及与炎症因子和肺功能的相关性
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作者 杨刚 周琴 赵雪林 《中国急救复苏与灾害医学杂志》 2024年第10期1349-1352,共4页
目的探讨慢性阻塞性肺疾病(COPD)患者血清肿瘤坏死因子相关凋亡诱导配体(TRAIL)与炎症因子和肺功能的关系。方法选取2020年6月—2022年12月在本院治疗的200例COPD患者为观察组,200例健康体检志愿者为对照组。根据COPD诊治指南中疾病类... 目的探讨慢性阻塞性肺疾病(COPD)患者血清肿瘤坏死因子相关凋亡诱导配体(TRAIL)与炎症因子和肺功能的关系。方法选取2020年6月—2022年12月在本院治疗的200例COPD患者为观察组,200例健康体检志愿者为对照组。根据COPD诊治指南中疾病类型分为急性加重期组140例,稳定期组60例。分析血清TRAIL水平与炎症因子[超敏-C反应蛋白(hs-CRP)、降钙素原(PCT)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平、肺功能[1秒用力呼气容积占比(FEV_(1)%)、FEV_(1)和用力肺活量(FVC)比值、肺一氧化碳弥散量占比(DLCO%)及呼气峰流速(PEF)]的关系。结果与对照组相比,稳定期组、急性加重期组患者血清TRAIL、hs-CRP、PCT、IL-6、TNF-α水平显著升高(P<0.05),FEV_(1)%、FEV_(1)/FVC、DLCO%、PEF明显降低(P<0.05);急性加重期组较稳定期组患者血清TRAIL、hs-CRP、PCT、IL-6、TNF-α水平明显升高(P<0.05),FEV_(1)%、FEV_(1)/FVC、DLCO%、PEF显著降低(P<0.05);多因素线性回归分析结果显示,TRAIL水平与hs-CRP、TNF-α、FEV_(1)/FVC和DLCO水平有关(P<0.05);经Pearson法分析,COPD患者血清TRAIL水平与hs-CRP、TNF-α均呈正相关(r=0.412,0.527,P<0.05),与FEV_(1)/FVC、DLCO%均呈负相关(r=-0.594,-0.476,P<0.05)。结论COPD患者血清TRAIL水平升高,其与患者炎症因子和肺功能密切相关。 展开更多
关键词 慢性阻塞性肺疾病 trail 炎症因子 肺功能
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Factors determining changes in the network of marked hiking trails in the Sudetes
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作者 Krzysztof KOŁODZIEJCZYK 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1075-1099,共25页
Hiking trails are a basic type of tourist infrastructure,which,on the one hand,make areas available for tourist traffic,and on the other hand,can contribute to the protection of the natural environment(if they are wel... Hiking trails are a basic type of tourist infrastructure,which,on the one hand,make areas available for tourist traffic,and on the other hand,can contribute to the protection of the natural environment(if they are well designed and maintained).Owing to the variety of performed functions,their designation is determined by several factors:natural,technical,economic,social.Networks of trails change constantly.The aim of this article is to determine exactly what factors influence transformations within the hiking trail networks and what is their significance.To this end,three study areas in the Sudetes were analysed:one on the Polish side of the Sudetes-the Table(Stołowe)Mountains,and two on the Czech side-the rock town near the village of Sloup vČechách and the central part of Zlatohorskávrchovina.An analysis of changes in the shape of the networks over time was carried out,as well as surveys of institutions that were responsible for or influenced these transformations.These areas are characterised by a significant level of changes in the trail network.Among the factors influencing these changes,the tourist attractiveness of the area,the resilience of the environment,the intensity of tourism traffic,the environmental transformations associated with it,the history of tourism development and land ownership changes should be considered the most important.At each stage of forming networks,the key factor should be tourists’needs,including the desire to escape the urbanised environment.For this reason,trails should avoid roads with artificial(hard)surfaces and heavy automobile traffic. 展开更多
关键词 Hiking trails trail networks Graphs Tourist traffic SUDETES
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Research on Developing an Assessment Scale for Tourism Experience Elements of Ancient Shu Road Heritage Trail
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作者 Wang Yuan Wang Bo Zhou Jiang 《Contemporary Social Sciences》 2024年第5期118-142,共25页
Cultural ancient roads,known in Chinese as gudao,serve as heritage trails that carry historical exchanges across various regions in China.Due to their extensive preservation,wide geographical distribution,diverse them... Cultural ancient roads,known in Chinese as gudao,serve as heritage trails that carry historical exchanges across various regions in China.Due to their extensive preservation,wide geographical distribution,diverse thematic variations,and considerable tourist appeal,these paths have emerged as representative heritage trails,increasingly transforming into a novel tourism product experience that is highly favored by tourists and recognized by government authorities.However,research on ancient roads for tourism in China currently lacks a systematic theoretical framework,as well as relevant policies,regulations,and standards to guide their practical development.Therefore,there is a pressing need to draw upon international best practices and conduct foundational research to develop an experience element system that aligns with the perceptions,behaviors,and consumption characteristics of Chinese tourists,thereby advancing theoretical exploration in this field.This study focuses on the representative Ancient Shu Road as a case study and employs a mixed-method approach that integrates qualitative and quantitative research.It aims to construct a tourist-centric scale for the experience elements of ancient road tourism while analyzing the interactive relationship between these experience elements and tourist needs.This study addresses a significant gap in the development of indicator systems for domestic studies of ancient road tourism experiences.Ultimately,the study establishes a comprehensive scale that encompasses three core categories—trail resources and environment,facilities and services,and modes of tourism activities—along with eight primary dimensions:core resources,surrounding cultural environment,surrounding natural environment,tourism reception facilities and services,infrastructure and support services,information facilities and information services,and outdoor and recreational activities.This scale consists of thirty-two specific items,providing a robust reference for future research endeavors.Additionally,the study proposes specific development strategies related to key mechanisms,spatial configuration,and facility construction to enhance the overall development of ancient road tourism. 展开更多
关键词 ancient roads heritage trails tourism experience elements scale development
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Performance of the walking trail making test in older adults with white matter hyperintensities
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作者 Hong-Yi Zhao Zhi-Qiang Zhang +2 位作者 Yong-Hua Huang Hong Li Fang-Yuan Wei 《World Journal of Psychiatry》 SCIE 2024年第1期102-110,共9页
BACKGROUND Several studies have reported that the walking trail making test(WTMT)completion time is significantly higher in patients with developmental coordination disorders and mild cognitive impairments.We hypothes... BACKGROUND Several studies have reported that the walking trail making test(WTMT)completion time is significantly higher in patients with developmental coordination disorders and mild cognitive impairments.We hypothesized that WTMT performance would be altered in older adults with white matter hyperintensities(WMH).AIM To explore the performance in the WTMT in older people with WMH.METHODS In this single-center,observational study,25 elderly WMH patients admitted to our hospital from June 2019 to June 2020 served as the WMH group and 20 participants matched for age,gender,and educational level who were undergoing physical examination in our hospital during the same period served as the control group.The participants completed the WTMT-A and WTMT-B to obtain their gait parameters,including WTMT-A completion time,WTMT-B completion time,speed,step length,cadence,and stance phase percent.White matter lesions were scored according to the Fazekas scale.Multiple neuropsychological assessments were carried out to assess cognitive function.The relationships between WTMT performance and cognition and motion in elderly patients with WMH were analyzed by partial Pearson correlation analysis.RESULTS Patients with WMH performed significantly worse on the choice reaction test(CRT)(0.51±0.09 s vs 0.44±0.06 s,P=0.007),verbal fluency test(VFT,14.2±2.75 vs 16.65±3.54,P=0.012),and digit symbol substitution test(16.00±2.75 vs 18.40±3.27,P=0.010)than participants in the control group.The WMH group also required significantly more time to complete the WTMT-A(93.00±10.76 s vs 70.55±11.28 s,P<0.001)and WTMT-B(109.72±12.26 s vs 82.85±7.90 s,P<0.001).WTMT-A completion time was positively correlated with CRT time(r=0.460,P=0.001),while WTMT-B completion time was negatively correlated with VFT(r=-0.391,P=0.008).On the WTMT-A,only speed was found to statistically differ between the WMH and control groups(0.803±0.096 vs 0.975±0.050 m/s,P<0.001),whereas on the WTMT-B,the WMH group exhibited a significantly lower speed(0.778±0.111 vs 0.970±0.053 m/s,P<0.001)and cadence(82.600±4.140 vs 85.500±5.020 steps/m,P=0.039),as well as a higher stance phase percentage(65.061±1.813%vs 63.513±2.465%,P=0.019)relative to controls.CONCLUSION Older adults with WMH showed obviously poorer WTMT performance.WTMT could be a potential indicator for cognitive and motor deficits in patients with WMH. 展开更多
关键词 White matter hyperintensities Cognitive dysfunction Motor deficits Gait analysis trail making test Small vessel disease
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Xiamen Mountains-to-Sea Trail
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作者 吕静钰 郭金涛(指导) 《疯狂英语(初中天地)》 2024年第9期72-73,共2页
Xiamen,a coastal city famous for its impressive seascape,draws numerous tourists each year.One of the prime locations to appreciate the city’s beauty is the Mountains-to-Sea Trail,an approximately 23-kilometer-long p... Xiamen,a coastal city famous for its impressive seascape,draws numerous tourists each year.One of the prime locations to appreciate the city’s beauty is the Mountains-to-Sea Trail,an approximately 23-kilometer-long pedestrian corridor that offers a unique experience for visitors. 展开更多
关键词 XIAMEN trail COASTAL
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血清TRAIL水平与晚期非小细胞肺癌患者免疫治疗反应的关联性研究
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作者 张雅君 张振安 +2 位作者 王继华 周媛 梁凤霞 《中国临床新医学》 2024年第6期645-650,共6页
目的探讨血清肿瘤坏死因子相关凋亡诱导配体(TRAIL)水平与晚期非小细胞肺癌(NSCLC)患者免疫治疗反应的关联性。方法回顾性分析2019年1月至2020年5月唐山市人民医院收治的70例晚期NSCLC患者的临床资料。在免疫治疗前24 h内采用酶联免疫... 目的探讨血清肿瘤坏死因子相关凋亡诱导配体(TRAIL)水平与晚期非小细胞肺癌(NSCLC)患者免疫治疗反应的关联性。方法回顾性分析2019年1月至2020年5月唐山市人民医院收治的70例晚期NSCLC患者的临床资料。在免疫治疗前24 h内采用酶联免疫吸附试验法(ELISA)测定患者血清TRAIL水平。免疫治疗反应通过客观缓解率(ORR)和临床获益率(CBR)进行评估。分析患者血清TRAIL水平与免疫治疗反应、肺功能及肺气肿、临床预后的关联性。结果经免疫治疗后,NSCLC患者获得客观缓解23例,临床获益46例。获得客观缓解患者的血清TRAIL水平显著高于未获得客观缓解者[27.77(23.13,39.13)pg/mL vs 12.36(8.76,18.15)pg/mL;Z=4.508,P<0.001]。临床获益患者的血清TRAIL水平显著高于临床未获益者[23.13(16.99,30.63)pg/mL vs 11.75(8.76,15.56)pg/mL;Z=4.887,P<0.001]。Spearman秩相关性分析结果显示,血清TRAIL水平与1秒用力呼气容积/用力肺活量(FEV1/FVC)呈正相关(rs=0.288,P=0.016),与肺气肿总比值(rs=-0.257,P=0.032)、肺叶肺气肿比率(LER)(rs=-0.324,P=0.006)呈负相关。多因素logistic回归分析结果显示,以血清TRAIL>27.46 pg/mL为参考,血清TRAIL<18.15 pg/mL患者经免疫治疗不能获得客观缓解和临床获益的风险显著增加(P<0.05)。ROC曲线分析结果显示,血清TRAIL水平可有效预测NSCLC患者对免疫治疗的反应(P<0.05)。TRAIL高水平组(血清TRAIL≥18.15 pg/mL)的总体生存(OS)、无进展生存(PFS)预后显著优于TRAIL低水平组(血清TRAIL<18.15 pg/mL)(P<0.05)。结论血清TRAIL低水平与晚期NSCLC患者免疫治疗无反应以及临床预后不良有关,该指标监测有助于筛选能从免疫治疗中获益的NSCLC患者。 展开更多
关键词 肿瘤坏死因子相关凋亡诱导配体 晚期非小细胞肺癌 免疫治疗反应 肺气肿 预后
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rNDV-IL-2和rNDV-TRAIL治疗肿瘤的协同作用
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作者 李思明 盛美琳 +1 位作者 吴毓斌 白福良 《哈尔滨商业大学学报(自然科学版)》 CAS 2024年第3期273-280,共8页
通过比较rNDV表达的白细胞介素-2(IL-2)和肿瘤坏死因子相关凋亡诱导配体(TRAIL)治疗肿瘤的效果,并进一步探讨联合应用rNDV-IL-2和rNDV-TRAIL是否在肿瘤治疗中产生协同效应.体外培养H22肝癌细胞株和B16-F10黑色素瘤细胞株,并建立小鼠H22... 通过比较rNDV表达的白细胞介素-2(IL-2)和肿瘤坏死因子相关凋亡诱导配体(TRAIL)治疗肿瘤的效果,并进一步探讨联合应用rNDV-IL-2和rNDV-TRAIL是否在肿瘤治疗中产生协同效应.体外培养H22肝癌细胞株和B16-F10黑色素瘤细胞株,并建立小鼠H22肝癌和B16-F10黑色素瘤动物模型,注射rNDV-IL-2和rNDV-TRAIL病毒进行治疗.同时注射人IL-2和TRAIL的重组新城疫病毒(rNDV-IL-2+rNDV-TRAIL)显著提高了rNDV通过诱导细胞凋亡来杀伤肿瘤细胞的能力.小鼠肝癌和恶性黑色素模型鼠的治疗实验表明,与对照相比,rNDV-IL-2或rNDV-TRAIL显著抑制了肿瘤的生长并延长荷瘤小鼠的存活.与rNDV-IL-2和rNDV-TRAIL治疗组相比较,rNDV-IL-2+rNDV-TRAIL联合治疗进一步增强了rNDV的抗肿瘤功效,并进一步延长了动物的存活时间.动物实验结果还表明,荷瘤鼠rNDV-IL-2和rNDV-IL-2+rNDV-TRAIL治疗后均可诱导系小鼠体内的CD4^(+)细胞和CD8^(+)细胞的增殖,并且rNDV-IL-2+rNDV-TRAIL联合治疗表现出明显的协同作用.rNDV-IL-2+rNDV-TRAIL联合治疗在体内外均能够明显抑制肿瘤增殖,较单一应用rNDV-IL-2或rNDV-TRAIL有更好的效果,值得进一步深入研究. 展开更多
关键词 重组新城疫病毒 癌症治疗 白细胞介素-2 肿瘤坏死因子相关凋亡诱导配体 协同治疗
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Research Note: A Multi-Method Approach to Monitor Recreational Trail Usage in Complex Spatial Settings
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作者 Sharon A. Jones Alex Junge Ruth Dittrich 《Open Journal of Forestry》 2023年第1期1-12,共12页
Trail use by pedestrians has become more popular in the United States over the last decade although few studies explore the use of technology to monitor high use trails. Monitoring trail users is an important part of ... Trail use by pedestrians has become more popular in the United States over the last decade although few studies explore the use of technology to monitor high use trails. Monitoring trail users is an important part of trail management and an optimal monitoring system usually depends on site-specific characteristics. The objective of this study was to demonstrate how using a multi-methods system to monitor backcountry trail usage in complex spatial settings can be a useful approach for collecting the information that trail managers need. Given the national growth in recreational hiking, we were particularly interested in exploring these issues for highly visited trails close to urban areas and selected a portion of the Larch Trail leading to the top of Multnomah Falls for the study. The multi-methods approach that we used included a combination of automated infrared sensor counts, manual counts, parking lot data from an inductive loop, and travel time estimates collected with low-energy Bluetooth sensors. We found that using multiple methods allowed for a cost-effective and rich data set that considered the site characteristics and the specific need of the trail managers. We expect that many backcountry trail settings have complex landscape and physical design without robust pre-existing baseline data and hope that our insights will aid trail managers as they strive for a sustainable balance between human use and landscape impact. 展开更多
关键词 Visitor Behavior trail Monitoring Pedestrian trails Infrared Sensors
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帕博利珠联合紫杉醇激活TRAILR/TRAIL促进食管癌细胞凋亡 被引量:1
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作者 尹宏飞 柴立勋 +1 位作者 王玉璇 杨更朴 《中国免疫学杂志》 CAS CSCD 北大核心 2023年第4期783-786,共4页
目的:初步探索帕博利珠联合紫杉醇促进食管癌细胞凋亡的机制。方法:qRT-PCR和Western blot检测食管癌组织和细胞中TRAILR2表达,以及食管癌细胞中caspase3和caspase8表达。CCK-8检测紫杉醇对食管癌细胞存活的影响。紫杉醇处理食管癌细胞... 目的:初步探索帕博利珠联合紫杉醇促进食管癌细胞凋亡的机制。方法:qRT-PCR和Western blot检测食管癌组织和细胞中TRAILR2表达,以及食管癌细胞中caspase3和caspase8表达。CCK-8检测紫杉醇对食管癌细胞存活的影响。紫杉醇处理食管癌细胞的同时过表达TRAILR2,鉴定紫杉醇对TRAILR/TRAIL途径的激活作用。构建小鼠食管癌异种移植模型,流式细胞术检测帕博利珠和紫杉醇对食管癌细胞凋亡的影响。结果:TRAILR2在食管癌组织和细胞中均正常表达。紫杉醇可有效抑制食管癌细胞存活,并激活TRAILR/TRAIL途径。紫杉醇可诱导食管癌细胞凋亡,而紫杉醇处理的同时过表达TRAILR2,细胞凋亡情况有所恢复。帕博利珠和紫杉醇均能抑制食管癌实体瘤体积和重量,而帕博利珠和紫杉醇联合处理可进一步增强这种抑制作用。流式细胞术结果表明,帕博利珠和紫杉醇联合处理促进食管癌细胞凋亡。结论:帕博利珠联合紫杉醇可通过TRAILR/TRAIL途径促进食管癌细胞凋亡。 展开更多
关键词 帕博利珠 紫杉醇 trailR/trail 食管癌 细胞凋亡
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TRAIL在HCC治疗研究中的新进展
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作者 黄妮 唐科 陈薇 《华夏医学》 CAS 2023年第5期179-185,共7页
肝细胞癌(HCC)是当今社会最常见的恶性肿瘤之一。手术切除仍是目前主要的治疗方法,但术后存在死亡率高、预后差等问题,因此寻求更加安全、高效的治疗策略尤为重要。肿瘤坏死因子相关凋亡诱导配体(TRAIL)是肿瘤坏死因子(TNF)家族的新成... 肝细胞癌(HCC)是当今社会最常见的恶性肿瘤之一。手术切除仍是目前主要的治疗方法,但术后存在死亡率高、预后差等问题,因此寻求更加安全、高效的治疗策略尤为重要。肿瘤坏死因子相关凋亡诱导配体(TRAIL)是肿瘤坏死因子(TNF)家族的新成员之一,相关受体在治疗肿瘤方面具有良好的优势,是具有抗肿瘤作用的潜在靶点。近年来,基于TRAIL受体激动剂及其联合治疗策略已经投入临床HCC的治疗,并取得了不错的临床治疗预期。但随着HCC耐药的发展,针对现有机制的TRAIL受体激动剂似乎难以达到预期效果,因此深入探寻HCC耐药机制及其新的联合治疗策略,对于HCC治疗十分必要。本文主要对TRAIL在发挥治疗HCC中涉及的相关机制尤其是相关耐药机制以及近年临床上使用的TRAIL受体激动剂联合用药策略进行综述。 展开更多
关键词 trail蛋白 肝细胞癌 耐药性 联合用药
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组织蛋白酶B联合TRAIL诱导胃癌BGC-823细胞凋亡中的作用研究
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作者 查道德 涂龙霞 吴文涌 《包头医学院学报》 CAS 2023年第1期4-7,共4页
目的:观察组织蛋白酶B(CTSB)联合TRAIL诱导胃癌BGC-823细胞凋亡的作用。方法:BGC-823细胞传代培养。采用MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡率以及免疫印迹法(Western blot)检测Cathepsin B蛋白表达。结果:TRAIL对BGC-823细... 目的:观察组织蛋白酶B(CTSB)联合TRAIL诱导胃癌BGC-823细胞凋亡的作用。方法:BGC-823细胞传代培养。采用MTT法检测细胞增殖情况,流式细胞仪检测细胞凋亡率以及免疫印迹法(Western blot)检测Cathepsin B蛋白表达。结果:TRAIL对BGC-823细胞增殖有抑制作用,对细胞凋亡有促进作用(P<0.05);Cathepsin B抑制剂浓度越高,细胞凋亡率越低(P<0.05);Western blot结果表明,Cathepsin B抑制剂浓度越高,Cathepsin B表达越少(P<0.05)。结论:Cathepsin B和TRAIL联合使用有协同诱导胃腺癌BGC-823细胞凋亡作用。 展开更多
关键词 组织蛋白酶B 肿瘤坏死因子 凋亡配体 BGC-823细胞 细胞凋亡
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FEM1B促进TRAIL诱导的HIV潜伏库细胞ACH-2的细胞凋亡
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作者 杨晨波 陆佳涵 +1 位作者 丛喆 薛婧 《中国比较医学杂志》 CAS 北大核心 2023年第5期1-7,共7页
目的探究FEM1B对HIV潜伏库细胞ACH-2的凋亡影响及作用。方法利用慢病毒转染构建FEM1B过表达的ACH-2细胞株,采用FITC-Annexin-V/7-AAD染色检测FEM1B过表达的ACH-2细胞株的细胞自发凋亡以及TRAIL作用24 h后的细胞凋亡情况,以及用qPCR检测T... 目的探究FEM1B对HIV潜伏库细胞ACH-2的凋亡影响及作用。方法利用慢病毒转染构建FEM1B过表达的ACH-2细胞株,采用FITC-Annexin-V/7-AAD染色检测FEM1B过表达的ACH-2细胞株的细胞自发凋亡以及TRAIL作用24 h后的细胞凋亡情况,以及用qPCR检测TRAIL作用前后细胞上清病毒载量。结果FEM1B过表达的细胞株的细胞自发凋亡没有显著变化,但在TRAIL作用下,过表达FEM1B的细胞株的凋亡率显著增加,同时FEM1B过表达后TRAIL作用下ACH-2的上清病毒载量明显上升。结论FEM1B能够促进TRAIL作用下HIV潜伏细胞的细胞凋亡和病毒载量。 展开更多
关键词 细胞凋亡 FEM1B trail HIV潜伏库
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Astrometric Reduction of Saturnian Satellites with Cassini-ISS Images Degraded by Trailed Stars
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作者 Qing-Feng Zhang Meng-Qi Liu +4 位作者 Yan Li Lin-Peng Wu Zhi-Qiang Wang Li-Sha Zhu Zhan Li 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2023年第3期85-92,共8页
The Imaging Science Subsystem(ISS)mounted on the Cassini spacecraft has taken a lot of images,which provides an important source of high-precision astrometry of some planets and satellites.However,some of these images... The Imaging Science Subsystem(ISS)mounted on the Cassini spacecraft has taken a lot of images,which provides an important source of high-precision astrometry of some planets and satellites.However,some of these images are degraded by trailed stars.Previously,these degraded images cannot be used for astrometry.In this paper,a new method is proposed to detect and compute the centers of these trailed stars automatically.The method is then performed on the astrometry of ISS images with trailed stars.Finally,we provided 658 astrometric positions between 2004 and 2017 of several satellites that include Enceladus,Dione,Tethys,Mimas and Rhea.Compared with the JPL ephemeris SAT427,the mean residuals of these measurements are 0.11 km and 0.26 km in R.A.and decl.,respectively.Their standard deviations are 1.08 km and 1.37 km,respectively.The results show that the proposed method performs astrometric measurements of Cassini ISS images with trailed stars effectively. 展开更多
关键词 STARS trail SATURN
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TRAIL基因通过PI3K/Akt信号通路抑制LPS诱导的A549凋亡和炎症反应
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作者 陈静 王栋宇 +1 位作者 何萌萌 管洁 《中国老年学杂志》 CAS 北大核心 2023年第12期3032-3036,共5页
目的探讨过表达肿瘤坏死因子凋亡相关诱导配体(TRAIL)基因对脂多糖(LPS)诱导的人肺泡Ⅱ型上皮细胞(A549)凋亡和炎症反应的影响及可能的机制。方法采用LPS处理A549构建急性肺损伤(ALI)体外细胞模型,实时荧光定量聚合酶链式反应(RT-qPCR)... 目的探讨过表达肿瘤坏死因子凋亡相关诱导配体(TRAIL)基因对脂多糖(LPS)诱导的人肺泡Ⅱ型上皮细胞(A549)凋亡和炎症反应的影响及可能的机制。方法采用LPS处理A549构建急性肺损伤(ALI)体外细胞模型,实时荧光定量聚合酶链式反应(RT-qPCR)和Western印迹测定TRAIL的表达变化。在A549中转染TRAIL-pcDNA3.1过表达载体质粒,RT-qPCR和Western印迹检测LPS诱导条件下的转染效果,CCK-8实验检测细胞活性,流式细胞术测定凋亡变化,Western印迹检测细胞中活化的半胱天冬酶(Cleaved Caspase)-3和Cleaved Caspase-9表达水平及磷脂酰肌醇3-激酶(PI3K)和蛋白激酶B(Akt)的磷酸化水平,酶联免疫吸附试验(ELISA)检测肿瘤坏死因子(TNF)-α、细胞间黏附分子(ICAM)-1和白细胞介素(IL)-8的含量。结果TRAIL mRNA和蛋白及A549活性在LPS处理后显著降低(P<0.05),A549凋亡率、Cleaved Caspase-3和Cleaved Caspase-9表达、p-PI3K和p-Akt水平及TNF-α、ICAM-1和IL-8含量均显著升高(P<0.05)。过表达TRAIL能够明显提高LPS诱导的A549活性,明显降低LPS诱导的细胞凋亡率、Cleaved Caspase-3和Cleaved Caspase-9表达、p-PI3K和p-Akt水平及TNF-α、ICAM-1和IL-8含量(P<0.05)。结论过表达TRAIL基因可降低LPS诱导的A549凋亡及炎症反应,其作用机制可能与阻断PI3K/Akt信号通路有关。 展开更多
关键词 trail基因 肺泡上皮细胞(A549) 炎症反应 急性肺损伤 PI3K/AKT信号通路
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子痫前期患者血浆中sTRAIL、胎盘组织TRAIL及TRAIL受体的变化及意义 被引量:3
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作者 牟纯玮 芮塬 +3 位作者 谢晓珍 周尚谦 李雪媛 王志坚 《现代妇产科进展》 CSCD 北大核心 2016年第12期895-899,共5页
目的:研究子痫前期(PE)患者血浆s TRAIL及胎盘滋养细胞上TRAIL/TRAIL-Rs的变化及其与PE发病的关系。方法:选取56例PE患者为研究对象,其中符合重度PE诊断标准者31例(研究组1),未及重度PE 25例(研究组2)。选取同期正常妊娠妇女为对照组,其... 目的:研究子痫前期(PE)患者血浆s TRAIL及胎盘滋养细胞上TRAIL/TRAIL-Rs的变化及其与PE发病的关系。方法:选取56例PE患者为研究对象,其中符合重度PE诊断标准者31例(研究组1),未及重度PE 25例(研究组2)。选取同期正常妊娠妇女为对照组,其中28~36周30例(对照组1)、36^(+1)~38周30例(对照组2),因胎膜早破、早产等于28~36周分娩者27例(对照组3),36^(+1)~38周分娩者30例(对照组4)。研究组孕妇留取静脉血及胎盘组织,对照组1、2留取静脉血,对照组3、4留取胎盘组织。ELISA法检测血浆中TRAIL含量,免疫组化法检测胎盘组织中TRAIL和TRAIL-Rs(DR4、DR5、DcR1、DcR2)含量。结果:研究组血浆中sTRAIL含量明显低于对照组(研究组1 vs对照组1,P<0.001;研究组2 vs对照组2,P<0.005);随着病情加重,s TRAIL含量明显下降(P<0.05)。研究组胎盘组织中TRAIL表达明显减少,随着病情加重而加重。研究组中死亡受体DR4、DR5的含量明显高于对照组,而诱骗受体DcR1、DcR2含量明显低于对照组。PE重度组中DR4、DR5含量高于轻度组(P<0.05),DcR1和DcR2含量则无显著差异。结论:PE患者血浆中sTRAIL及胎盘组织中TRAIL/TRAIL-Rs含量发生了明显变化,TRAIL/TRAIL-Rs的不平衡性参与了PE的病理过程。 展开更多
关键词 子痫前期 血浆strail 胎盘trail trail-Rs
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TRAIL受体在肿瘤细胞系上的表达及意义 被引量:8
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作者 韩丽辉 孙汶生 +3 位作者 马春红 张利宁 曹英林 宋静 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2002年第2期128-130,共3页
目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡... 目的  检测TNF相关凋亡诱导配体(TRAIL)的受体,在来源于血液系统、肝脏、肺脏和大肠的8个肿瘤细胞系中的表达,并探讨其意义。方法采用半定量RT-PCR,对TRAIL受体的表达进行半定量检测。结果 TRAIL凋亡通路中,能够诱导凋亡反应的死亡受体DR4和DR5,在所检测的肿瘤细胞系中都有表达,其中DR5在所有肿瘤细胞系中的表达水平均显著高于DR4(P<0.05)。而能够竞争性阻断TRAIL诱导的凋亡反应的诱骗受体DcR1和DcR2,在所有的肿瘤细胞中都呈低水平表达或不表达。结论 DR5可能在TRAIL诱导凋亡的通路中发挥最重要的作用。TRAIL死亡受体和诱骗受体在肿瘤细胞系中的表达具有差异性,这种差异性可在一定程度上解释不同细胞对TRAIL诱导凋亡的敏感度。 展开更多
关键词 肿瘤 RT-PCR trail trail受体
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全人源抗TRAIL-R1/-R2单克隆抗体肿瘤治疗的研究进展 被引量:2
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作者 宗卫娇 芦凤亮 +1 位作者 刘晔 金艾顺 《实用肿瘤学杂志》 CAS 2014年第4期344-348,共5页
目前全人源TRAIL受体(TRAIL-Rs)的单克隆抗体(单抗)对肿瘤靶向治疗已进入临床Ⅰ~Ⅳ期研究。TRAIL-Rs单抗可分别与死亡受体TRAIL-R1和TRAIL-R2结合,形成死亡诱导信号复合体(DISC),诱导肿瘤细胞凋亡,成为抗肿瘤抗体药物研发的... 目前全人源TRAIL受体(TRAIL-Rs)的单克隆抗体(单抗)对肿瘤靶向治疗已进入临床Ⅰ~Ⅳ期研究。TRAIL-Rs单抗可分别与死亡受体TRAIL-R1和TRAIL-R2结合,形成死亡诱导信号复合体(DISC),诱导肿瘤细胞凋亡,成为抗肿瘤抗体药物研发的热点之一。全人源TRAIL-Rs单抗与放疗、化疗药物或其它制剂以及免疫治疗方法联合应用在肿瘤治疗中取得了显著的效果,为临床抗肿瘤治疗提供了新方案。 展开更多
关键词 trail trail受体 trail-Rs单抗 肿瘤治疗
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TRAIL与妇科肿瘤的治疗 被引量:6
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作者 李新国 杨华 徐碧泉 《中国现代医学杂志》 CAS CSCD 2004年第2期50-52,58,共4页
关键词 trail trail受体 妇科肿瘤 治疗
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胃癌细胞TRAIL受体的表达与其对TRAIL敏感性的关系 被引量:3
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作者 吴萍 张旭东 张林杰 《现代生物医学进展》 CAS 2008年第7期1286-1288,1298,共4页
目的:探讨胃癌细胞表面TRAIL受体表达水平及其与TRAIL敏感性的关系。方法:PI染色、流式细胞仪检测TRAIL诱导BGC-823及SGC-7901细胞的凋亡率,流式细胞仪检测细胞膜表面四种TRAIL受体-R1、R2、R3、R4的表达情况。结果:TRAIL诱导胃癌细胞... 目的:探讨胃癌细胞表面TRAIL受体表达水平及其与TRAIL敏感性的关系。方法:PI染色、流式细胞仪检测TRAIL诱导BGC-823及SGC-7901细胞的凋亡率,流式细胞仪检测细胞膜表面四种TRAIL受体-R1、R2、R3、R4的表达情况。结果:TRAIL诱导胃癌细胞凋亡具有剂量和时间依赖性,BGC-823较SGC-7901对TRAIL诱导的凋亡更敏感,TRAIL(100μg·L^(-1))作用24 h的细胞凋亡率分别是59.9%、24.3%。死亡受体TRAIL-R1/DR4、TRAIL-R2/DR5在BGC-823细胞膜表面表达的阳性率高达97.87%和99.42%,而在SGC-7901分别为7.03%和95.31%,诱骗受体TRAIL-R3/DcR1、TRAIL-R4/DcR2在两株细胞膜表面极少表达。结论:胃癌细胞对TRAIL诱导凋亡的敏感性差异可能与细胞膜表面死亡受体有关,尤其与DR4的表达有关。 展开更多
关键词 胃肿瘤 细胞凋亡 trail trail受体
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TRAIL及其受体的研究进展 被引量:3
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作者 范清林 宋礼华 《中国生化药物杂志》 CAS CSCD 2001年第2期103-105,共3页
TRAIL(TNFrelatedapoptosis inducingligand)及其受体属于肿瘤坏死因子超基因家族成员 ,在细胞的凋亡调控中起着重要的作用。TRAIL能诱导多种肿瘤细胞的凋亡 ,而正常组织细胞对其不敏感 ,提示TRAIL可能成为一种新的抗肿瘤药物。
关键词 trail trail受体 细胞凋亡 肿瘤细胞
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