Debonding at the cement-casing interface is recognized as a principal failure mechanism leading to CO_(2) leakage in wells.This detachment gives rise to a microannulus,which notably possesses greater permeability than...Debonding at the cement-casing interface is recognized as a principal failure mechanism leading to CO_(2) leakage in wells.This detachment gives rise to a microannulus,which notably possesses greater permeability than undamaged cement,undermining its sealing efficacy.Conventionally,the permeability of the microannulus is regarded as a uniform value throughout the well.However,fundamentally,a microannulus is one type of fracture,and its gap or aperture size is affected by the effective stress.In this work,we developed a unique experimental apparatus.This equipment facilitates the curing of cement inside a steel casing,the formation of a microannulus between the casing and the cement,and the investigation of the fluid flow dynamics along the microannulus under laboratory-replicated in situ conditions.The microannulus was formed by injecting fluid from one end of the setup,and receiving similar amount of fluid on the other end signified the development of the leakage channel.Additionally,strain gauges affixed to the casing’s external surface yielded key information on the microannulus’s opening and closure.We observed a noticeable decline in microannulus hydraulic aperture(or permeability)in relation to effective stress and an exponential equation fits their relationship.Our findings also indicate a distinct behavior when comparing liquid CO_(2) with water.Specifically,it is easier for liquid CO_(2) to create the microannulus.However,the hydraulic aperture range for this microannulus(0.7-6 mm)is considerably smaller than that created by water flow(2-17 mm).Finally,we integrated the stressdependent microannulus aperture size into the combined analysis of well mechanical integrity and well leakage.The outcomes consistently demonstrated that when factoring in the stress-dependent aperture sizes,the leakage rates are 3e5 times compared to a fixed aperture model.The traditional assumption of a constant aperture significantly underestimates fluid leakage risks.展开更多
The aperture of natural rock fractures significantly affects the deformation and strength properties of rock masses,as well as the hydrodynamic properties of fractured rock masses.The conventional measurement methods ...The aperture of natural rock fractures significantly affects the deformation and strength properties of rock masses,as well as the hydrodynamic properties of fractured rock masses.The conventional measurement methods are inadequate for collecting data on high-steep rock slopes in complex mountainous regions.This study establishes a high-resolution three-dimensional model of a rock slope using unmanned aerial vehicle(UAV)multi-angle nap-of-the-object photogrammetry to obtain edge feature points of fractures.Fracture opening morphology is characterized using coordinate projection and transformation.Fracture central axis is determined using vertical measuring lines,allowing for the interpretation of aperture of adaptive fracture shape.The feasibility and reliability of the new method are verified at a construction site of a railway in southeast Tibet,China.The study shows that the fracture aperture has a significant interval effect and size effect.The optimal sampling length for fractures is approximately 0.5e1 m,and the optimal aperture interpretation results can be achieved when the measuring line spacing is 1%of the sampling length.Tensile fractures in the study area generally have larger apertures than shear fractures,and their tendency to increase with slope height is also greater than that of shear fractures.The aperture of tensile fractures is generally positively correlated with their trace length,while the correlation between the aperture of shear fractures and their trace length appears to be weak.Fractures of different orientations exhibit certain differences in their distribution of aperture,but generally follow the forms of normal,log-normal,and gamma distributions.This study provides essential data support for rock and slope stability evaluation,which is of significant practical importance.展开更多
In electromagnetic countermeasures circumstances,synthetic aperture radar(SAR)imagery usually suffers from severe quality degradation from modulated interrupt sampling repeater jamming(MISRJ),which usually owes consid...In electromagnetic countermeasures circumstances,synthetic aperture radar(SAR)imagery usually suffers from severe quality degradation from modulated interrupt sampling repeater jamming(MISRJ),which usually owes considerable coherence with the SAR transmission waveform together with periodical modulation patterns.This paper develops an MISRJ suppression algorithm for SAR imagery with online dictionary learning.In the algorithm,the jamming modulation temporal properties are exploited with extracting and sorting MISRJ slices using fast-time autocorrelation.Online dictionary learning is followed to separate real signals from jamming slices.Under the learned representation,time-varying MISRJs are suppressed effectively.Both simulated and real-measured SAR data are also used to confirm advantages in suppressing time-varying MISRJs over traditional methods.展开更多
Photogrammetry,reconstructing three-dimensional(3D)models from overlapping two-dimensional(2D)photos,finds application in rock mechanics and rock engineering to extract geometrical details of reconstructed objects,for...Photogrammetry,reconstructing three-dimensional(3D)models from overlapping two-dimensional(2D)photos,finds application in rock mechanics and rock engineering to extract geometrical details of reconstructed objects,for example rock fractures.Fracture properties are important for determining the mechanical stability,permeability,strength,and shear behavior of the rock mass.Photogrammetry can be used to reconstruct detailed 3D models of two separated rock fracture surfaces to characterize fracture roughness and physical aperture,which controls the fluid flow,hydromechanical and shear behavior of the rock mass.This research aimed to determine the optimal number of scale bars required to produce high-precision 3D models of a fracture surface.A workflow has been developed to define the physical aperture of a fracture using photogrammetry.Three blocks of Kuru granite(25 cm×25 cm×10 cm)with an artificially induced fracture,were investigated.For scaling 3D models,321 markers were used as ground control points(GCPs)with predefined distances on each block.When the samples were wellmatched in their original positions,the entire block was photographed.Coordinate data of the GCPs were extracted from the 3D model of the blocks.Each half was surveyed separately and georeferenced by GCPs and merged into the same coordinate system.Two fracture surfaces were extracted from the 3D models and the vertical distance between the two surfaces was digitally calculated as physical aperture.Accuracy assessment of the photogrammetric reconstruction showed a 20-30 mm digital control distance accuracy when compared to known distances defined between markers.To attain this accuracy,the study found that at least 200 scale bars were required.Furthermore,photogrammetry was employed to measure changes in aperture under normal stresses.The results obtained from this approach were found to be in good agreement with those obtained using linear variable displacement transducers(LVDTs),with differences ranging from 1 mm to 8μm.展开更多
Obtaining precise position of interested emitters passively has wide applications in both civilian and military fields.Different from traditional parameter measurement and direct position determination(DPD)method,rece...Obtaining precise position of interested emitters passively has wide applications in both civilian and military fields.Different from traditional parameter measurement and direct position determination(DPD)method,recently a new passive localization method based on synthetic aper-ture technique,named synthetic aperture positioning(SAP),has been proposed.The method com-pensates for the nonlinear phase produced by relative motion between the moving platform and the emitter,achieving coherent summation of intercepted signals.The SAP can obtain high-resolution and high-precision localization results at a low signal-to-noise ratio.This paper summarizes the research progress of SAP,including localization principles,spaceborne applications,and application scope analysis.Besides,the possible future outlook of SAP is considered.展开更多
The existing direction-of-arrival(DOA)estimation methods only utilize the current received signals,which are susceptible to noise.In this paper,a method for DOA estimation based on a motion platform is proposed to ach...The existing direction-of-arrival(DOA)estimation methods only utilize the current received signals,which are susceptible to noise.In this paper,a method for DOA estimation based on a motion platform is proposed to achieve high-precision DOA estimation by utilizing past and present signals.The concept of synthetic aperture is introduced to construct a linear DOA estima-tion model.A DOA fine-tuning method based on the linear model is proposed to eliminate the lin-ear DOA variation,achieving a non-coherent accumulation of DOA estimations.Moreover,the baseband modulation and the phase modulation caused by the range history are compensated to achieve the coherent accumulation of all the DOA estimations.Simulation results show that the proposed method can significantly improve the DOA estimated accuracy at low signal-to-noise ratios(SNR).展开更多
The primary mirrors of current and future large telescopes always employ a segmented mirror configuration.The small but non-negligible gaps between neighboring segments cause additional diffraction,which restricts the...The primary mirrors of current and future large telescopes always employ a segmented mirror configuration.The small but non-negligible gaps between neighboring segments cause additional diffraction,which restricts the performance of high-contrast coronagraph.To solve this problem,we propose a coronagraph system based on a single liquid crystal spatial light modulator(SLM).This spatial light modulator is used for amplitude apodization,and its feasibility and potential performance are demonstrated using a laboratory setup using the stochastic parallel gradient descent(SPGD)algorithm to control the spatial light modulator,which is based on point spread function(PSF)sensing and evaluation and optimized for maximum contrast in the discovery working area as a merit function.The system delivers a contrast in the order of 10−6,and shows excellent potential to be used in current and future large aperture telescopes,both on the ground and in space.展开更多
BACKGROUND Alveolar bone defects caused by inflammation are an urgent issue in oral implant surgery that must be solved.Regulating the various phenotypes of macrophages to enhance the inflammatory environment can sign...BACKGROUND Alveolar bone defects caused by inflammation are an urgent issue in oral implant surgery that must be solved.Regulating the various phenotypes of macrophages to enhance the inflammatory environment can significantly affect the progression of diseases and tissue engineering repair process.AIM To assess the influence of interleukin-10(IL-10)on the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)following their interaction with macrophages in an inflammatory environment.METHODS IL-10 modulates the differentiation of peritoneal macrophages in Wistar rats in an inflammatory environment.In this study,we investigated its impact on the proliferation,migration,and osteogenesis of BMSCs.The expression levels of signal transducer and activator of transcription 3(STAT3)and its activated form,phos-phorylated-STAT3,were examined in IL-10-stimulated macrophages.Subsequently,a specific STAT3 signaling inhibitor was used to impede STAT3 signal activation to further investigate the role of STAT3 signaling.RESULTS IL-10-stimulated macrophages underwent polarization to the M2 type through substitution,and these M2 macrophages actively facilitated the osteogenic differentiation of BMSCs.Mechanistically,STAT3 signaling plays a crucial role in the process by which IL-10 influences macrophages.Specifically,IL-10 stimulated the activation of the STAT3 signaling pathway and reduced the macrophage inflammatory response,as evidenced by its diminished impact on the osteogenic differentiation of BMSCs.CONCLUSION Stimulating macrophages with IL-10 proved effective in improving the inflammatory environment and promoting the osteogenic differentiation of BMSCs.The IL-10/STAT3 signaling pathway has emerged as a key regulator in the macrophage-mediated control of BMSCs’osteogenic differentiation.展开更多
Managing server lost circulation is a major challenge of drilling operation in naturally fractured formations and it causes much nonproductive rig time. When encountered with loss, the fracture aperture intersecting t...Managing server lost circulation is a major challenge of drilling operation in naturally fractured formations and it causes much nonproductive rig time. When encountered with loss, the fracture aperture intersecting the wellbore is not well-identified in time, which has a significant impact on the decision of drilling operation and the undesired result of loss curing. Therefore, the onset of fracture is identified in a timely manner and evaluated comprehensively to formulate an appropriate strategy over time. However, the mud loss date, which is the primary source of information retrieved from the drilling process, was not properly used in real-time prediction of fracture aperture. This article provides a detailed mathematical study to discuss the mechanism of mud invasion in the near-wellbore region and prediction of fracture aperture. The fracture aperture can be calculated from mud-loss data by solving a cubic equation with input parameters given by the well radius, the overpressure ratio, and the maximum mud-loss volume. It permits the proper selection of loss-circulation material (LCM) with respect to particle size distribution and fiber usage. The case study illustrates the applicability of this methodology with a discussion on LCM particle distribution in different scenarios and the result demonstrates the outcome of inappropriate LCM usage and the advantages of the novel fiber-based LCM treatment.展开更多
In order to improve the operability and accuracy of high-intensity focused ultrasound （HIFU）, an annular focused transducer, whereby a B-ultrasound probe is placed in its center, is used to realize the real time mon...In order to improve the operability and accuracy of high-intensity focused ultrasound （HIFU）, an annular focused transducer, whereby a B-ultrasound probe is placed in its center, is used to realize the real time monitoring and control of the treatment. In this paper, the spheroidal beam equation （SBE） was used to calculate the sound lield by an annular focused transducer with a wide aperture angle to first derive the heat deposition and the Pennes equation was used to calculate the temperature field in multi-layer tissue. We studied the effect of different parameters on the temperature of the tissues. The result shows that the focal length has a significant influence on both maximum liver temperature rise and skin temperature rise, and both increase with the increase in the focal length. When the frequency increases, the temperature rise first undergoes a rapid increase before gradually reaching a maximum, and then finally decreasing. The temperature rise increases while the inner radius decreases or the sound pressure increases. By choosing suitable parameters, the proper temperature rise both on the target tissue and skin via an annular tbcused transducer with a wide aperture angle can be obtained.展开更多
Main quadrupole magnets are critical for the Circular Electron and Positron Collider(CEPC)and are specifically designed as dual aperture quadrupole(DAQ)magnets.However,the field crosstalk between the two apertures pre...Main quadrupole magnets are critical for the Circular Electron and Positron Collider(CEPC)and are specifically designed as dual aperture quadrupole(DAQ)magnets.However,the field crosstalk between the two apertures presents challenges.As the CEPC will work at four beam energies of Z,W,Higgs and ttbar mode,the DAQ magnets will operate at four field gradients spanning from 3.18 to 12.63 T/m.The first short quadrupole magnet prototype with the bore diameter of 76 mm and magnetic length of 1.0 m revealed the problems of large magnetic field harmonics and a magnetic center shift within the beam energy range.Accordingly,a compensation method was proposed in this work to solve the field crosstalk effect.By adjusting the gap height at the middle of the two apertures,the field harmonics and magnetic center shift are significantly reduced.After optimization,the short prototype was modified using a new scheme.The field simulations are validated from the magnetic measurement results.Further,the multipole field meets the requirements of the four beam energies.The detailed magnetic field optimization,field harmonics adjustment,and measurement results are presented herein.展开更多
High resolution imaging is achieved using increasingly larger apertures and successively shorter wavelengths.Optical aperture synthesis is an important high-resolution imaging technology used in astronomy.Conventional...High resolution imaging is achieved using increasingly larger apertures and successively shorter wavelengths.Optical aperture synthesis is an important high-resolution imaging technology used in astronomy.Conventional long baseline amplitude interferometry is susceptible to uncontrollable phase fluctuations,and the technical difficulty increases rapidly as the wavelength decreases.The intensity interferometry inspired by HBT experiment is essentially insensitive to phase fluctuations,but suffers from a narrow spectral bandwidth which results in a lack of effective photons.In this study,we propose optical synthetic aperture imaging based on spatial intensity interferometry.This not only realizes diffraction-limited optical aperture synthesis in a single shot,but also enables imaging with a wide spectral bandwidth,which greatly improves the optical energy efficiency of intensity interferometry.And this method is insensitive to the optical path difference between the sub-apertures.Simulations and experiments present optical aperture synthesis diffraction-limited imaging through spatial intensity interferometry in a 100 nm spectral width of visible light,whose maximum optical path difference between the sub-apertures reaches 69λ.This technique is expected to provide a solution for optical aperture synthesis over kilometer-long baselines at optical wavelengths.展开更多
The gradient element of the aperture gradient map is utilized directly to generate the aperture shape without modulation.This process can be likened to choosing the direction of negative gradient descent for the gener...The gradient element of the aperture gradient map is utilized directly to generate the aperture shape without modulation.This process can be likened to choosing the direction of negative gradient descent for the generic aperture shape optimiza-tion.The negative gradient descent direction is more suitable under local conditions and has a slow convergence rate.To overcome these limitations,this study introduced conjugate gradients into aperture shape optimization based on gradient modulation.First,the aperture gradient map of the current beam was obtained for the proposed aperture shape optimiza-tion method,and the gradients of the aperture gradient map were modulated using conjugate gradients to form a modulated gradient map.The aperture shape was generated based on the modulated gradient map.The proposed optimization method does not change the optimal solution of the original optimization problem,but changes the iterative search direction when generating the aperture shape.The performance of the proposed method was verified using cases of head and neck cancer,and prostate cancer.The optimization results indicate that the proposed optimization method better protects the organs at risk and rapidly reduces the objective function value by ensuring a similar dose distribution to the planning target volume.Compared to the contrasting methods,the normal tissue complication probability obtained by the proposed optimization method decreased by up to 4.61%,and the optimization time of the proposed method decreased by 5.26%on average for ten cancer cases.The effectiveness and acceleration of the proposed method were verified through comparative experiments.According to the comparative experiments,the results indicate that the proposed optimization method is more suitable for clinical applications.It is feasible for the aperture shape optimization involving the proposed method.展开更多
The characteristics of traditional acoustic vortices(AVs) were verified by the cross-sectional axisymmetric pressure distributions with perfect phase spirals around the center pressure null. In order to generate a non...The characteristics of traditional acoustic vortices(AVs) were verified by the cross-sectional axisymmetric pressure distributions with perfect phase spirals around the center pressure null. In order to generate a non-axisymmetric pressure distribution, the concept of power-exponent-phase was first introduced into the formation of AV beams, named “powerexponent-phase acoustic vortices(PAVs)” in this paper. Based on a ring-array of sector transducers, the helical distribution of the low-pressure valley in cross-sections of PAVs, which enables particles to move from a distant position to the center low-potential well along a certain spiral passageway, was proved theoretically. The particle manipulation behavior for PAVs with a power order of 2 is numerically modeled and experimentally confirmed. The results show that PAVs with a nonaxisymmetric spiral pressure distribution can be used to realize the directional transport of particles in an enlarged scope,suggesting prospective application potential in biomedical engineering.展开更多
Optical cavity has long been critical for a variety of applications ranging from precise measurement to spectral analysis.A number of theories and methods have been successful in describing the optical response of a s...Optical cavity has long been critical for a variety of applications ranging from precise measurement to spectral analysis.A number of theories and methods have been successful in describing the optical response of a stratified optical cavity,while the inverse problem,especially the inverse design of a displacement sensitive cavity,remains a significant challenge due to the cost of computation and comprehensive performance requirements.This paper reports a novel inverse design methodology combining the characteristic matrix method,mixed-discrete variables optimization algorithm,and Monte Carlo method-based tolerance analysis.The material characteristics are indexed to enable the mixed-discrete variables optimization,which yields considerable speed and efficiency improvements.This method allows arbitrary response adjustment with technical feasibility and gives a glimpse into the analytical characterization of the optical response.Two entirely different light-displacement responses,including an asymmetric sawtooth-like response and a highly symmetric response,are dug out and experimentally achieved,which fully confirms the validity of the method.The compact Fabry-Perot cavities have a good balance between performance and feasibility,making them promising candidates for displacement transducers.More importantly,the proposed inverse design paves the way for a universal design of optical cavities,or even nanophotonic devices.展开更多
Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming metho...Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming methods.Although ultrasonic transducers with longitudinal vibration have been widely studied,they have seldom been incorporated into UPF devices for machining in confined spaces.To meet the requirements of this type of machining,a sandwich-type piezoelectric transducer with coupled longitudinal-flexural vibrational modes is proposed.The basic structure of the transducer is designed to obtain large vibrational amplitudes in both modes.Experimental results obtained with a prototype device demonstrate the feasibility of the proposed transducer.The measured vibrational amplitude for the working face in the longitudinal vibrational mode is 1.0μm,and electrical matching increases this amplitude by 40%.The flexural vibration characteristics of the same prototype transducer are also tested and are found to be slightly smaller than those of longitudinal mode.The resultant working strokes of the UPF impact pins reach 1.7 mm and 1.2 mm in the longitudinal and flexural modes,respectively.The forming capability of the prototype has been evaluated via 15-min machining on standard 2024-T351 aluminum plates.After UPF,an improved surface morphology with lower surface roughness is obtained.The aluminum plate test piece has an apparent upper deformation with an arc height of 0.64 mm.The measured peak value of the compressive residual stress is around 250 MPa,appearing at a depth of 100μm.The proposed longitudinal-flexural hybrid transducer thus provides a high-performance tool for plate peen forming in confined spaces.展开更多
BACKGROUND Gastric cancer(GC)is one of the most common cancer types worldwide,and its prevention and treatment methods have garnered much attention.As the active ingredient of licorice,18β-glycyrrhetinic acid(18β-GR...BACKGROUND Gastric cancer(GC)is one of the most common cancer types worldwide,and its prevention and treatment methods have garnered much attention.As the active ingredient of licorice,18β-glycyrrhetinic acid(18β-GRA)has a variety of pharmacological effects.The aim of this study was to explore the effective target of 18β-GRA in the treatment of GC,in order to provide effective ideas for the clinical prevention and treatment of GC.AIM To investigate the mechanism of 18β-GRA in inhibiting cell proliferation and promoting autophagy flux in GC cells.METHODS Whole transcriptomic analyses were used to analyze and screen differentially expressed microRNAs(miRNAs)in GC cells after 18β-GRA intervention.Lentivirus-transfected GC cells and the Cell Counting Kit-8 were used to detect cell proliferation ability,cell colony formation ability was detected by the clone formation assay,and flow cytometry was used to detect the cell cycle and apoptosis.A nude mouse transplantation tumor model of GC cells was constructed to verify the effect of miR-328-3p overexpression on the tumorigenicity of GC cells.Tumor tissue morphology was observed by hematoxylin and eosin staining,and microtubule-associated protein light chain 3(LC3)expression was detected by immunohistochemistry.TransmiR,STRING,and miRWalk databases were used to predict the relationship between miR-328-3p and signal transducer and activator of transcription 3(STAT3)-related information.Expression of STAT3 mRNA and miR-328-3p was detected by quantitative polymerase chain reaction(qPCR)and the expression levels of STAT3,phosphorylated STAT3(p-STAT3),and LC3 were detected by western blot analysis.The targeted relationship between miR-328-3p and STAT3 was detected using the dual-luciferase reporter gene system.AGS cells were infected with monomeric red fluorescent protein-green fluorescent protein-LC3 adenovirus double label.LC3 was labeled and autophagy flow was observed under a confocal laser microscope.RESULTS The expression of miR-328-3p was significantly upregulated after 18β-GRA intervention in AGS cells(P=4.51E-06).Overexpression of miR-328-3p inhibited GC cell proliferation and colony formation ability,arrested the cell cycle in the G0/G1 phase,promoted cell apoptosis,and inhibited the growth of subcutaneous tumors in BALB/c nude mice(P<0.01).No obvious necrosis was observed in the tumor tissue in the negative control group(no drug intervention or lentivirus transfection)and vector group(the blank vector for lentivirus transfection),and more cells were loose and necrotic in the miR-328-3p group.Bioinformatics tools predicted that miR-328-3p has a targeting relationship with STAT3,and STAT3 was closely related to autophagy markers such as p62.After overexpressing miR-328-3p,the expression level of STAT3 mRNA was significantly decreased(P<0.01)and p-STAT3 was downregulated(P<0.05).The dual-luciferase reporter gene assay showed that the luciferase activity of miR-328-3p and STAT33’untranslated regions of the wild-type reporter vector group was significantly decreased(P<0.001).Overexpressed miR-328-3p combined with bafilomycin A1(Baf A1)was used to detect the expression of LC3 II.Compared with the vector group,the expression level of LC3 II in the overexpressed miR-328-3p group was downregulated(P<0.05),and compared with the Baf A1 group,the expression level of LC3 II in the overexpressed miR-328-3p+Baf A1 group was upregulated(P<0.01).The expression of LC3 II was detected after intervention of 18β-GRA in GC cells,and the results were consistent with the results of miR-328-3p overexpression(P<0.05).Additional studies showed that 18β-GRA promoted autophagy flow by promoting autophagosome synthesis(P<0.001).qPCR showed that the expression of STAT3 mRNA was downregulated after drug intervention(P<0.05).Western blot analysis showed that the expression levels of STAT3 and p-STAT3 were significantly downregulated after drug intervention(P<0.05).CONCLUSION 18β-GRA promotes the synthesis of autophagosomes and inhibits GC cell proliferation by regulating the miR-328-3p/STAT3 signaling pathway.展开更多
The large aperture optical mirror for space is processed and tested in the gravity environment on the ground. After entering space, gravity disappears due to the change of environment, and the mirror surface that has ...The large aperture optical mirror for space is processed and tested in the gravity environment on the ground. After entering space, gravity disappears due to the change of environment, and the mirror surface that has met the engineering requirements on the ground will change, seriously affecting the imaging quality. In order to eliminate the influence of gravity and to ensure the consistency of space and ground, gravity unloading must be performed. In order to meet the requirements of processing and testing for the large aperture space mirror in the state of vertical optical axis, a universal gravity unloading device was proposed. It was an active support and used air cylinders to provide accurate unloading force. First, the design flow of gravity unloading was introduced;then the detailed design of the mechanical structure and control system was given;then the performance parameters of the two types of cylinders were tested and compared, including the force-pressure relationship curve and the force-position relationship curve;finally, the experimental verification of the gravity unloading device was carried out;for a mirror with an aperture of ?2100 mm, the gravity unloading device was designed and a vertical detection optical path was built. The test results showed that by using this gravity unloading device, the actual processing surface accuracy of the mirror was better than 1/50λ-RMS, which met the application requirement of the optical system. Thus, it can be seen that using this gravity unloading device can effectively unload the gravity of the mirror and realize the accurate processing and measurement of the mirror surface. .展开更多
基金Financial support for this work from the U.S.Department of Energy(DOE)Office of Basic Energy Sciences for“Center for Coupled Chemo-Mechanics of Cementitious Composites for EGS(C4M)”,DOE’s“National Risk Assessment Partnership(NRAP)”programDOE Office of Energy Efficiency&Renewable Energy’s Geothermal Technologies Office for“Advanced Downhole Acoustic Sensing for Wellbore Integrity”is gratefully acknowledged.
文摘Debonding at the cement-casing interface is recognized as a principal failure mechanism leading to CO_(2) leakage in wells.This detachment gives rise to a microannulus,which notably possesses greater permeability than undamaged cement,undermining its sealing efficacy.Conventionally,the permeability of the microannulus is regarded as a uniform value throughout the well.However,fundamentally,a microannulus is one type of fracture,and its gap or aperture size is affected by the effective stress.In this work,we developed a unique experimental apparatus.This equipment facilitates the curing of cement inside a steel casing,the formation of a microannulus between the casing and the cement,and the investigation of the fluid flow dynamics along the microannulus under laboratory-replicated in situ conditions.The microannulus was formed by injecting fluid from one end of the setup,and receiving similar amount of fluid on the other end signified the development of the leakage channel.Additionally,strain gauges affixed to the casing’s external surface yielded key information on the microannulus’s opening and closure.We observed a noticeable decline in microannulus hydraulic aperture(or permeability)in relation to effective stress and an exponential equation fits their relationship.Our findings also indicate a distinct behavior when comparing liquid CO_(2) with water.Specifically,it is easier for liquid CO_(2) to create the microannulus.However,the hydraulic aperture range for this microannulus(0.7-6 mm)is considerably smaller than that created by water flow(2-17 mm).Finally,we integrated the stressdependent microannulus aperture size into the combined analysis of well mechanical integrity and well leakage.The outcomes consistently demonstrated that when factoring in the stress-dependent aperture sizes,the leakage rates are 3e5 times compared to a fixed aperture model.The traditional assumption of a constant aperture significantly underestimates fluid leakage risks.
基金This work was supported by the National Nature Science Foundation of China(Grant Nos.42177139 and 41941017)the Natural Science Foundation Project of Jilin Province,China(Grant No.20230101088JC).The authors would like to thank the anonymous reviewers for their comments and suggestions.
文摘The aperture of natural rock fractures significantly affects the deformation and strength properties of rock masses,as well as the hydrodynamic properties of fractured rock masses.The conventional measurement methods are inadequate for collecting data on high-steep rock slopes in complex mountainous regions.This study establishes a high-resolution three-dimensional model of a rock slope using unmanned aerial vehicle(UAV)multi-angle nap-of-the-object photogrammetry to obtain edge feature points of fractures.Fracture opening morphology is characterized using coordinate projection and transformation.Fracture central axis is determined using vertical measuring lines,allowing for the interpretation of aperture of adaptive fracture shape.The feasibility and reliability of the new method are verified at a construction site of a railway in southeast Tibet,China.The study shows that the fracture aperture has a significant interval effect and size effect.The optimal sampling length for fractures is approximately 0.5e1 m,and the optimal aperture interpretation results can be achieved when the measuring line spacing is 1%of the sampling length.Tensile fractures in the study area generally have larger apertures than shear fractures,and their tendency to increase with slope height is also greater than that of shear fractures.The aperture of tensile fractures is generally positively correlated with their trace length,while the correlation between the aperture of shear fractures and their trace length appears to be weak.Fractures of different orientations exhibit certain differences in their distribution of aperture,but generally follow the forms of normal,log-normal,and gamma distributions.This study provides essential data support for rock and slope stability evaluation,which is of significant practical importance.
基金supported by the National Natural Science Foundation of China(61771372,61771367,62101494)the National Outstanding Youth Science Fund Project(61525105)+1 种基金Shenzhen Science and Technology Program(KQTD20190929172704911)the Aeronautic al Science Foundation of China(2019200M1001)。
文摘In electromagnetic countermeasures circumstances,synthetic aperture radar(SAR)imagery usually suffers from severe quality degradation from modulated interrupt sampling repeater jamming(MISRJ),which usually owes considerable coherence with the SAR transmission waveform together with periodical modulation patterns.This paper develops an MISRJ suppression algorithm for SAR imagery with online dictionary learning.In the algorithm,the jamming modulation temporal properties are exploited with extracting and sorting MISRJ slices using fast-time autocorrelation.Online dictionary learning is followed to separate real signals from jamming slices.Under the learned representation,time-varying MISRJs are suppressed effectively.Both simulated and real-measured SAR data are also used to confirm advantages in suppressing time-varying MISRJs over traditional methods.
基金funding provided by the State Nuclear Waste Management Fund(VYR)and the support of the Ministry of Economic Affairs and Employment of Finland on the Finnish Research Program on Nuclear Waste Management KYT2018 and KYT2022 of the Nuclear Energy Act(990/1987)in the research projects Fluid flow in fractured hard rock mass(RAKKA),funding numbers KYT 1/2021 and KYT 1/2022Additional support was received from the National Nuclear Safety and Waste Management Research Program SAFER2028,funding numbers SAFER 25/2023(MIRKA)and SAFER 42/2023(CORF).
文摘Photogrammetry,reconstructing three-dimensional(3D)models from overlapping two-dimensional(2D)photos,finds application in rock mechanics and rock engineering to extract geometrical details of reconstructed objects,for example rock fractures.Fracture properties are important for determining the mechanical stability,permeability,strength,and shear behavior of the rock mass.Photogrammetry can be used to reconstruct detailed 3D models of two separated rock fracture surfaces to characterize fracture roughness and physical aperture,which controls the fluid flow,hydromechanical and shear behavior of the rock mass.This research aimed to determine the optimal number of scale bars required to produce high-precision 3D models of a fracture surface.A workflow has been developed to define the physical aperture of a fracture using photogrammetry.Three blocks of Kuru granite(25 cm×25 cm×10 cm)with an artificially induced fracture,were investigated.For scaling 3D models,321 markers were used as ground control points(GCPs)with predefined distances on each block.When the samples were wellmatched in their original positions,the entire block was photographed.Coordinate data of the GCPs were extracted from the 3D model of the blocks.Each half was surveyed separately and georeferenced by GCPs and merged into the same coordinate system.Two fracture surfaces were extracted from the 3D models and the vertical distance between the two surfaces was digitally calculated as physical aperture.Accuracy assessment of the photogrammetric reconstruction showed a 20-30 mm digital control distance accuracy when compared to known distances defined between markers.To attain this accuracy,the study found that at least 200 scale bars were required.Furthermore,photogrammetry was employed to measure changes in aperture under normal stresses.The results obtained from this approach were found to be in good agreement with those obtained using linear variable displacement transducers(LVDTs),with differences ranging from 1 mm to 8μm.
基金supported in part by the National Science Fund for Excellent Young Scholars(No.62222113)in part by the joint Funds of the National Natural Science Foundation of China(No.U22B2015)+1 种基金in part by the stabilization support of National Radar Signal Processing Laboratory(No.KGJ202203)in part by the Fundamental Research Funds for the Central Universities(No.ZDRC2004).
文摘Obtaining precise position of interested emitters passively has wide applications in both civilian and military fields.Different from traditional parameter measurement and direct position determination(DPD)method,recently a new passive localization method based on synthetic aper-ture technique,named synthetic aperture positioning(SAP),has been proposed.The method com-pensates for the nonlinear phase produced by relative motion between the moving platform and the emitter,achieving coherent summation of intercepted signals.The SAP can obtain high-resolution and high-precision localization results at a low signal-to-noise ratio.This paper summarizes the research progress of SAP,including localization principles,spaceborne applications,and application scope analysis.Besides,the possible future outlook of SAP is considered.
基金supported in part by the National Science Fund for Excel-lent Young Scholars(No.62222113)in part by the joint Funds of the National Natural Science Foundation of China(No.U22B2015)+1 种基金in part by the stabilization support of National Radar Signal Processing Laboratory(No.KGJ202203)in part by the Fundamental Research Funds for the Central Universities(No.ZDRC2004).
文摘The existing direction-of-arrival(DOA)estimation methods only utilize the current received signals,which are susceptible to noise.In this paper,a method for DOA estimation based on a motion platform is proposed to achieve high-precision DOA estimation by utilizing past and present signals.The concept of synthetic aperture is introduced to construct a linear DOA estima-tion model.A DOA fine-tuning method based on the linear model is proposed to eliminate the lin-ear DOA variation,achieving a non-coherent accumulation of DOA estimations.Moreover,the baseband modulation and the phase modulation caused by the range history are compensated to achieve the coherent accumulation of all the DOA estimations.Simulation results show that the proposed method can significantly improve the DOA estimated accuracy at low signal-to-noise ratios(SNR).
基金supported by the National Natural Science Foundation of China (U2031210 and 11827804)Science Research from the China Manned Space Project (CMS-CSST-2021-A11 and CMS-CSST-2021-B04).
文摘The primary mirrors of current and future large telescopes always employ a segmented mirror configuration.The small but non-negligible gaps between neighboring segments cause additional diffraction,which restricts the performance of high-contrast coronagraph.To solve this problem,we propose a coronagraph system based on a single liquid crystal spatial light modulator(SLM).This spatial light modulator is used for amplitude apodization,and its feasibility and potential performance are demonstrated using a laboratory setup using the stochastic parallel gradient descent(SPGD)algorithm to control the spatial light modulator,which is based on point spread function(PSF)sensing and evaluation and optimized for maximum contrast in the discovery working area as a merit function.The system delivers a contrast in the order of 10−6,and shows excellent potential to be used in current and future large aperture telescopes,both on the ground and in space.
文摘BACKGROUND Alveolar bone defects caused by inflammation are an urgent issue in oral implant surgery that must be solved.Regulating the various phenotypes of macrophages to enhance the inflammatory environment can significantly affect the progression of diseases and tissue engineering repair process.AIM To assess the influence of interleukin-10(IL-10)on the osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)following their interaction with macrophages in an inflammatory environment.METHODS IL-10 modulates the differentiation of peritoneal macrophages in Wistar rats in an inflammatory environment.In this study,we investigated its impact on the proliferation,migration,and osteogenesis of BMSCs.The expression levels of signal transducer and activator of transcription 3(STAT3)and its activated form,phos-phorylated-STAT3,were examined in IL-10-stimulated macrophages.Subsequently,a specific STAT3 signaling inhibitor was used to impede STAT3 signal activation to further investigate the role of STAT3 signaling.RESULTS IL-10-stimulated macrophages underwent polarization to the M2 type through substitution,and these M2 macrophages actively facilitated the osteogenic differentiation of BMSCs.Mechanistically,STAT3 signaling plays a crucial role in the process by which IL-10 influences macrophages.Specifically,IL-10 stimulated the activation of the STAT3 signaling pathway and reduced the macrophage inflammatory response,as evidenced by its diminished impact on the osteogenic differentiation of BMSCs.CONCLUSION Stimulating macrophages with IL-10 proved effective in improving the inflammatory environment and promoting the osteogenic differentiation of BMSCs.The IL-10/STAT3 signaling pathway has emerged as a key regulator in the macrophage-mediated control of BMSCs’osteogenic differentiation.
文摘Managing server lost circulation is a major challenge of drilling operation in naturally fractured formations and it causes much nonproductive rig time. When encountered with loss, the fracture aperture intersecting the wellbore is not well-identified in time, which has a significant impact on the decision of drilling operation and the undesired result of loss curing. Therefore, the onset of fracture is identified in a timely manner and evaluated comprehensively to formulate an appropriate strategy over time. However, the mud loss date, which is the primary source of information retrieved from the drilling process, was not properly used in real-time prediction of fracture aperture. This article provides a detailed mathematical study to discuss the mechanism of mud invasion in the near-wellbore region and prediction of fracture aperture. The fracture aperture can be calculated from mud-loss data by solving a cubic equation with input parameters given by the well radius, the overpressure ratio, and the maximum mud-loss volume. It permits the proper selection of loss-circulation material (LCM) with respect to particle size distribution and fiber usage. The case study illustrates the applicability of this methodology with a discussion on LCM particle distribution in different scenarios and the result demonstrates the outcome of inappropriate LCM usage and the advantages of the novel fiber-based LCM treatment.
基金Project supported by the National Key Research and Development Program,China(Grant No.2016YFF0203000)the National Natural Science Foundation of China(Grant Nos.11774167 and 61571222)+2 种基金the Fundamental Research Funds for the Central Universities,China(Grant No.020414380001)the State Key Laboratory of Acoustics,Chinese Academy of Sciences(Grant No.SKLA201609)AQSIQ Technology Research and Development Program,China(Grant No.2017QK125)
文摘In order to improve the operability and accuracy of high-intensity focused ultrasound （HIFU）, an annular focused transducer, whereby a B-ultrasound probe is placed in its center, is used to realize the real time monitoring and control of the treatment. In this paper, the spheroidal beam equation （SBE） was used to calculate the sound lield by an annular focused transducer with a wide aperture angle to first derive the heat deposition and the Pennes equation was used to calculate the temperature field in multi-layer tissue. We studied the effect of different parameters on the temperature of the tissues. The result shows that the focal length has a significant influence on both maximum liver temperature rise and skin temperature rise, and both increase with the increase in the focal length. When the frequency increases, the temperature rise first undergoes a rapid increase before gradually reaching a maximum, and then finally decreasing. The temperature rise increases while the inner radius decreases or the sound pressure increases. By choosing suitable parameters, the proper temperature rise both on the target tissue and skin via an annular tbcused transducer with a wide aperture angle can be obtained.
文摘Main quadrupole magnets are critical for the Circular Electron and Positron Collider(CEPC)and are specifically designed as dual aperture quadrupole(DAQ)magnets.However,the field crosstalk between the two apertures presents challenges.As the CEPC will work at four beam energies of Z,W,Higgs and ttbar mode,the DAQ magnets will operate at four field gradients spanning from 3.18 to 12.63 T/m.The first short quadrupole magnet prototype with the bore diameter of 76 mm and magnetic length of 1.0 m revealed the problems of large magnetic field harmonics and a magnetic center shift within the beam energy range.Accordingly,a compensation method was proposed in this work to solve the field crosstalk effect.By adjusting the gap height at the middle of the two apertures,the field harmonics and magnetic center shift are significantly reduced.After optimization,the short prototype was modified using a new scheme.The field simulations are validated from the magnetic measurement results.Further,the multipole field meets the requirements of the four beam energies.The detailed magnetic field optimization,field harmonics adjustment,and measurement results are presented herein.
基金supported by National Natural Foundation of China(Grant No.61991454)the project of CAS Interdisciplinary Innovation Team。
文摘High resolution imaging is achieved using increasingly larger apertures and successively shorter wavelengths.Optical aperture synthesis is an important high-resolution imaging technology used in astronomy.Conventional long baseline amplitude interferometry is susceptible to uncontrollable phase fluctuations,and the technical difficulty increases rapidly as the wavelength decreases.The intensity interferometry inspired by HBT experiment is essentially insensitive to phase fluctuations,but suffers from a narrow spectral bandwidth which results in a lack of effective photons.In this study,we propose optical synthetic aperture imaging based on spatial intensity interferometry.This not only realizes diffraction-limited optical aperture synthesis in a single shot,but also enables imaging with a wide spectral bandwidth,which greatly improves the optical energy efficiency of intensity interferometry.And this method is insensitive to the optical path difference between the sub-apertures.Simulations and experiments present optical aperture synthesis diffraction-limited imaging through spatial intensity interferometry in a 100 nm spectral width of visible light,whose maximum optical path difference between the sub-apertures reaches 69λ.This technique is expected to provide a solution for optical aperture synthesis over kilometer-long baselines at optical wavelengths.
基金supported by the Natural Science Foundation of Shanxi Province(No.20210302124403)the Research Project Supported by Shanxi Scholarship Council of China(No.2021-111)the Science and Technology Innovation Project of Colleges and Universities in Shanxi Province(No.2022L353).
文摘The gradient element of the aperture gradient map is utilized directly to generate the aperture shape without modulation.This process can be likened to choosing the direction of negative gradient descent for the generic aperture shape optimiza-tion.The negative gradient descent direction is more suitable under local conditions and has a slow convergence rate.To overcome these limitations,this study introduced conjugate gradients into aperture shape optimization based on gradient modulation.First,the aperture gradient map of the current beam was obtained for the proposed aperture shape optimiza-tion method,and the gradients of the aperture gradient map were modulated using conjugate gradients to form a modulated gradient map.The aperture shape was generated based on the modulated gradient map.The proposed optimization method does not change the optimal solution of the original optimization problem,but changes the iterative search direction when generating the aperture shape.The performance of the proposed method was verified using cases of head and neck cancer,and prostate cancer.The optimization results indicate that the proposed optimization method better protects the organs at risk and rapidly reduces the objective function value by ensuring a similar dose distribution to the planning target volume.Compared to the contrasting methods,the normal tissue complication probability obtained by the proposed optimization method decreased by up to 4.61%,and the optimization time of the proposed method decreased by 5.26%on average for ten cancer cases.The effectiveness and acceleration of the proposed method were verified through comparative experiments.According to the comparative experiments,the results indicate that the proposed optimization method is more suitable for clinical applications.It is feasible for the aperture shape optimization involving the proposed method.
基金Project supported by the Natural Science Foundation of Shandong Province, China (Grant Nos. ZR2022MF336 and ZR2022MD036)。
文摘The characteristics of traditional acoustic vortices(AVs) were verified by the cross-sectional axisymmetric pressure distributions with perfect phase spirals around the center pressure null. In order to generate a non-axisymmetric pressure distribution, the concept of power-exponent-phase was first introduced into the formation of AV beams, named “powerexponent-phase acoustic vortices(PAVs)” in this paper. Based on a ring-array of sector transducers, the helical distribution of the low-pressure valley in cross-sections of PAVs, which enables particles to move from a distant position to the center low-potential well along a certain spiral passageway, was proved theoretically. The particle manipulation behavior for PAVs with a power order of 2 is numerically modeled and experimentally confirmed. The results show that PAVs with a nonaxisymmetric spiral pressure distribution can be used to realize the directional transport of particles in an enlarged scope,suggesting prospective application potential in biomedical engineering.
基金We are grateful for financial supports from National Natural Science Foundation of China(62004166)Natural Science Foundation of Ningbo(202003N4062)+2 种基金National Postdoctoral Program for Innovative Talents(BX20200279)Natural Science Basic Research Program of Shaanxi Province(2020JQ-199)Fundamental Research Funds for the Central Universities(31020190QD027).
文摘Optical cavity has long been critical for a variety of applications ranging from precise measurement to spectral analysis.A number of theories and methods have been successful in describing the optical response of a stratified optical cavity,while the inverse problem,especially the inverse design of a displacement sensitive cavity,remains a significant challenge due to the cost of computation and comprehensive performance requirements.This paper reports a novel inverse design methodology combining the characteristic matrix method,mixed-discrete variables optimization algorithm,and Monte Carlo method-based tolerance analysis.The material characteristics are indexed to enable the mixed-discrete variables optimization,which yields considerable speed and efficiency improvements.This method allows arbitrary response adjustment with technical feasibility and gives a glimpse into the analytical characterization of the optical response.Two entirely different light-displacement responses,including an asymmetric sawtooth-like response and a highly symmetric response,are dug out and experimentally achieved,which fully confirms the validity of the method.The compact Fabry-Perot cavities have a good balance between performance and feasibility,making them promising candidates for displacement transducers.More importantly,the proposed inverse design paves the way for a universal design of optical cavities,or even nanophotonic devices.
基金supported by the National Natural Science Foundation of China(Grant Nos.51975278 and 52277055)the Qing Lan Project,the Research Fund of the State Key Laboratory of Mechanics and Control of Mechanical Structures(Nanjing University of Aeronautics and Astronautics)under Grant No.MCMS-I-0321G01+2 种基金the Biomedical Engineering Fusion Laboratory of the affiliated Jiangning Hospital of Nanjing Medical University(Grant No.JNYYZXKY202217)the Postgraduate Research&Practice Innovation Program of NUAA(Grant Nos.xcxjh20220114 and xcxjh20220111)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(Grant No.KYCX23_0353).
文摘Ultrasonic peen forming(UPF)is an emerging technology that exhibits great superiority in both its flexible operating modes and the deep residual stress that it produces compared with conventional plastic forming methods.Although ultrasonic transducers with longitudinal vibration have been widely studied,they have seldom been incorporated into UPF devices for machining in confined spaces.To meet the requirements of this type of machining,a sandwich-type piezoelectric transducer with coupled longitudinal-flexural vibrational modes is proposed.The basic structure of the transducer is designed to obtain large vibrational amplitudes in both modes.Experimental results obtained with a prototype device demonstrate the feasibility of the proposed transducer.The measured vibrational amplitude for the working face in the longitudinal vibrational mode is 1.0μm,and electrical matching increases this amplitude by 40%.The flexural vibration characteristics of the same prototype transducer are also tested and are found to be slightly smaller than those of longitudinal mode.The resultant working strokes of the UPF impact pins reach 1.7 mm and 1.2 mm in the longitudinal and flexural modes,respectively.The forming capability of the prototype has been evaluated via 15-min machining on standard 2024-T351 aluminum plates.After UPF,an improved surface morphology with lower surface roughness is obtained.The aluminum plate test piece has an apparent upper deformation with an arc height of 0.64 mm.The measured peak value of the compressive residual stress is around 250 MPa,appearing at a depth of 100μm.The proposed longitudinal-flexural hybrid transducer thus provides a high-performance tool for plate peen forming in confined spaces.
基金Ningxia Medical University Project,No. XZ2021005Ningxia Natural Science Foundation,Nos. 2022AAC03144 and 2022AAC02039National Natural Science Foundation of China,No. 82260879
文摘BACKGROUND Gastric cancer(GC)is one of the most common cancer types worldwide,and its prevention and treatment methods have garnered much attention.As the active ingredient of licorice,18β-glycyrrhetinic acid(18β-GRA)has a variety of pharmacological effects.The aim of this study was to explore the effective target of 18β-GRA in the treatment of GC,in order to provide effective ideas for the clinical prevention and treatment of GC.AIM To investigate the mechanism of 18β-GRA in inhibiting cell proliferation and promoting autophagy flux in GC cells.METHODS Whole transcriptomic analyses were used to analyze and screen differentially expressed microRNAs(miRNAs)in GC cells after 18β-GRA intervention.Lentivirus-transfected GC cells and the Cell Counting Kit-8 were used to detect cell proliferation ability,cell colony formation ability was detected by the clone formation assay,and flow cytometry was used to detect the cell cycle and apoptosis.A nude mouse transplantation tumor model of GC cells was constructed to verify the effect of miR-328-3p overexpression on the tumorigenicity of GC cells.Tumor tissue morphology was observed by hematoxylin and eosin staining,and microtubule-associated protein light chain 3(LC3)expression was detected by immunohistochemistry.TransmiR,STRING,and miRWalk databases were used to predict the relationship between miR-328-3p and signal transducer and activator of transcription 3(STAT3)-related information.Expression of STAT3 mRNA and miR-328-3p was detected by quantitative polymerase chain reaction(qPCR)and the expression levels of STAT3,phosphorylated STAT3(p-STAT3),and LC3 were detected by western blot analysis.The targeted relationship between miR-328-3p and STAT3 was detected using the dual-luciferase reporter gene system.AGS cells were infected with monomeric red fluorescent protein-green fluorescent protein-LC3 adenovirus double label.LC3 was labeled and autophagy flow was observed under a confocal laser microscope.RESULTS The expression of miR-328-3p was significantly upregulated after 18β-GRA intervention in AGS cells(P=4.51E-06).Overexpression of miR-328-3p inhibited GC cell proliferation and colony formation ability,arrested the cell cycle in the G0/G1 phase,promoted cell apoptosis,and inhibited the growth of subcutaneous tumors in BALB/c nude mice(P<0.01).No obvious necrosis was observed in the tumor tissue in the negative control group(no drug intervention or lentivirus transfection)and vector group(the blank vector for lentivirus transfection),and more cells were loose and necrotic in the miR-328-3p group.Bioinformatics tools predicted that miR-328-3p has a targeting relationship with STAT3,and STAT3 was closely related to autophagy markers such as p62.After overexpressing miR-328-3p,the expression level of STAT3 mRNA was significantly decreased(P<0.01)and p-STAT3 was downregulated(P<0.05).The dual-luciferase reporter gene assay showed that the luciferase activity of miR-328-3p and STAT33’untranslated regions of the wild-type reporter vector group was significantly decreased(P<0.001).Overexpressed miR-328-3p combined with bafilomycin A1(Baf A1)was used to detect the expression of LC3 II.Compared with the vector group,the expression level of LC3 II in the overexpressed miR-328-3p group was downregulated(P<0.05),and compared with the Baf A1 group,the expression level of LC3 II in the overexpressed miR-328-3p+Baf A1 group was upregulated(P<0.01).The expression of LC3 II was detected after intervention of 18β-GRA in GC cells,and the results were consistent with the results of miR-328-3p overexpression(P<0.05).Additional studies showed that 18β-GRA promoted autophagy flow by promoting autophagosome synthesis(P<0.001).qPCR showed that the expression of STAT3 mRNA was downregulated after drug intervention(P<0.05).Western blot analysis showed that the expression levels of STAT3 and p-STAT3 were significantly downregulated after drug intervention(P<0.05).CONCLUSION 18β-GRA promotes the synthesis of autophagosomes and inhibits GC cell proliferation by regulating the miR-328-3p/STAT3 signaling pathway.
文摘The large aperture optical mirror for space is processed and tested in the gravity environment on the ground. After entering space, gravity disappears due to the change of environment, and the mirror surface that has met the engineering requirements on the ground will change, seriously affecting the imaging quality. In order to eliminate the influence of gravity and to ensure the consistency of space and ground, gravity unloading must be performed. In order to meet the requirements of processing and testing for the large aperture space mirror in the state of vertical optical axis, a universal gravity unloading device was proposed. It was an active support and used air cylinders to provide accurate unloading force. First, the design flow of gravity unloading was introduced;then the detailed design of the mechanical structure and control system was given;then the performance parameters of the two types of cylinders were tested and compared, including the force-pressure relationship curve and the force-position relationship curve;finally, the experimental verification of the gravity unloading device was carried out;for a mirror with an aperture of ?2100 mm, the gravity unloading device was designed and a vertical detection optical path was built. The test results showed that by using this gravity unloading device, the actual processing surface accuracy of the mirror was better than 1/50λ-RMS, which met the application requirement of the optical system. Thus, it can be seen that using this gravity unloading device can effectively unload the gravity of the mirror and realize the accurate processing and measurement of the mirror surface. .
