In order to study the alkali resistivity of VHA-c in Iris lacteal. (IrlVHA-c), the transgenic tobacco seedlings harboring IrlVHA-c gene from Iris lactea (T0), the self-crossed progeny (T1), and the non-transgenic line...In order to study the alkali resistivity of VHA-c in Iris lacteal. (IrlVHA-c), the transgenic tobacco seedlings harboring IrlVHA-c gene from Iris lactea (T0), the self-crossed progeny (T1), and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol·L-1 NaHCO3 . The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol·L-1 NaHCO3 , and activity was slightly lower at 300 mmol·L-1 NaHCO3 . When the concentration of NaHCO3 was as high as 400 mmol·L-1 the seedlings were badly hurt. In addition, the activity of T0 and T1 transgentic tobacoo was maintained more or less. While the non-transgenic lines of tobacco could maintain viably up to 100 mmol·L-1 NaHCO3 , and they could not survive at 400 mmol·L-1 NaHCO3 . The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.展开更多
Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco....Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic to- bacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress.展开更多
A calmodulin-dependent protein kinase (MCK1) appeared important in regulating flowering in tobacco. The expression of modified MCK1 that lacks the C-terminal including calmodulin-binding domain upsets the flowering de...A calmodulin-dependent protein kinase (MCK1) appeared important in regulating flowering in tobacco. The expression of modified MCK1 that lacks the C-terminal including calmodulin-binding domain upsets the flowering developmental program, leading to the abortion of flower primordia initiated on the main axis of the plant and, as well, caused the prolongation of the vegetative phase in axillary buds. The abortion process of flowers began first in the developing anthers and subsequently the entire flower senesces. In axillary buds the prolonged vegetative phase was characterized by atypical elongated, narrow, twisted leaves. These results suggested a role for calmodulin-dependent protein kinase homologs in mediating flowering.展开更多
基金Supported by the Post-doctoral research start-up funding of Heilongjiang Province (LBH-210144)Postdoctoral Funds Project of Heilongjiang Province (520-415037)
文摘In order to study the alkali resistivity of VHA-c in Iris lacteal. (IrlVHA-c), the transgenic tobacco seedlings harboring IrlVHA-c gene from Iris lactea (T0), the self-crossed progeny (T1), and the non-transgenic lines of tobacco seedlings were grown in Hoagland nutrient solutions supplemented with 0, 100, 200, 300, 400 mmol·L-1 NaHCO3 . The MDA content, CAT, POD and SOD activity, electrical conductivity, chlorophyll content, soluble sugar content, proline content and polyphenol oxidase activity of the seedlings were determined. The results showed that the transgenic lines of tobacco maintained a high activity up to 200 mmol·L-1 NaHCO3 , and activity was slightly lower at 300 mmol·L-1 NaHCO3 . When the concentration of NaHCO3 was as high as 400 mmol·L-1 the seedlings were badly hurt. In addition, the activity of T0 and T1 transgentic tobacoo was maintained more or less. While the non-transgenic lines of tobacco could maintain viably up to 100 mmol·L-1 NaHCO3 , and they could not survive at 400 mmol·L-1 NaHCO3 . The conclusion was drawn that the alkali resistance of the tobacoo transformed IrlVHA-c was noticeably improved.
基金supported by the Natural Science Foundation of Jiangsu Province (No. BK2010344)the Opening Foundation of State Key Laboratory of Crop Biology (No. 2011KF11)+1 种基金the Postdoctoral Science Foundation of China (No. 2011M500867)the National Natural Science Foundation of China (No. 31071338)
文摘Ascorbate peroxidases are directly involved in reactive oxygen species (ROS) scavenging by reducing hydrogen peroxide to water. The tomato thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco. RNA gel blot analysis confirmed that StAPX in tomato leaves was induced by methylviologen-mediated oxidative stress. The sense transgenic seedlings exhibited higher tAPX activity than that of the wild type (WT) plants under oxidative stress conditions, while the antisense seedlings exhibited lower tAPX activity. Lower APX activities of antisense transgenic seedlings caused higher malondialdehyde contents and relative electrical conductivity. The sense transgenic seedlings with higher tAPX activity maintained higher chlorophyll content and showed the importance of tAPX in maintaining the optimal chloroplast development under methylviologen stress conditions, whereas the antisense lines maintained lower chlorophyll content than WT seedlings. Results indicated that the over-expression of StAPX enhanced tolerance to methylviologen-mediated oxidative stress in sense transgenic to- bacco early seedlings, whereas the suppression of StAPX in antisense transgenic seedlings showed high sensitivity to oxidative stress.
基金the National Basic Research Program (Grant No. G1999 0116), theNational Science Fund for Outstanding Youth (Grant No. 39725015), the National Natural Science Foundation of China (Grant No. 39780015), the National Fund for Transgenic Project (Grant No.
文摘A calmodulin-dependent protein kinase (MCK1) appeared important in regulating flowering in tobacco. The expression of modified MCK1 that lacks the C-terminal including calmodulin-binding domain upsets the flowering developmental program, leading to the abortion of flower primordia initiated on the main axis of the plant and, as well, caused the prolongation of the vegetative phase in axillary buds. The abortion process of flowers began first in the developing anthers and subsequently the entire flower senesces. In axillary buds the prolonged vegetative phase was characterized by atypical elongated, narrow, twisted leaves. These results suggested a role for calmodulin-dependent protein kinase homologs in mediating flowering.
