The effects of apple dwarf stocks P22 and SH3 on the juvenile phase of Fuji seedlings were studied. The results showed that the juvenile phase could be shortened by more than two years when 2-year seedlings were graft...The effects of apple dwarf stocks P22 and SH3 on the juvenile phase of Fuji seedlings were studied. The results showed that the juvenile phase could be shortened by more than two years when 2-year seedlings were grafted on the dwarf stocks. The effects of P22 and SH3 were significantly different. Grafted on P22, the flowering node was less, the first flowing site was lower, and the juvenile phase was shorter than SH3. With the two stocks, the average first flowering site was more than 200 cm, the average flowering node was more than 100, and the lateral branch top bud was more than 50% in all first flowing seedlings.展开更多
The production technique of apple hybrid seedlings was systematically summarized, and the technology regulations were made, including rootstock cultivation, interstock grafting, hybrid seeds preparation and hybrid see...The production technique of apple hybrid seedlings was systematically summarized, and the technology regulations were made, including rootstock cultivation, interstock grafting, hybrid seeds preparation and hybrid seedlings grafting. This technical specification provides a unified technical specification for hybrid apple seedling breeders, and is of important guiding significance for the production of apple in Hebei Province.展开更多
This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effect...This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effects of crops such as Allium fistulosum, Brassica juncea, and Triticum aestivum on four species of Fusarium were observed and compared in six treatments. These were continuous cropping(CK), fumigation with the methyl bromide(FM), rotating A. fistulosum only(R1), rotating A. fistulosum and T. aestivum(R2), rotating A. fistulosum, B. juncea, and T. aestivum(R3), and fallow(FC) in a year. The results showed that the biomass of Malus hupehensis Rehd. seedlings increased significantly. The root length increased and the root architecture was optimized. The respiration rate of the root system was increased by about 1 time after rotation. The treatments of R1, R2, R3, and FC increased bacterial count by 232.17%, 96.04%, 316.21%, and 60.02%, respectively. However, the fungi were reduced in varying degrees and bacteria/fungi ratio was increased by 5–10 times. The enzyme activities, p H, and organic matter were increased, but soil bulk density was decreased. Phenolic acids such as phloridzin was decreased significantly. The copy number of four Fusarium species declined by 85.59%, 74.94%, 69.68%, and 54.41% after rotating three different crops(R3 treatment). The root volatiles of three plants inhibited mycelial growth and spore germination of four Fusarium species.展开更多
Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapi...Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.展开更多
By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety diff...By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.展开更多
[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials...[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA （with addition of 0.1 mg/L NAA）, fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS ＋ BA 1.2 mg/L ＋ NAA 0. 1 mg/L ＋ sucrose 30 g/L ＋ agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.展开更多
Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, th...Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, the differences in the expression levels of 3 invertase genes, CWI, SAI and NI, between virus- free and ordinary sugarcane seedlings were analyzed. Compared with ordinary sugarcane plants, the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage, and especially, greatly up-regulated in immature interuedes at maturation stage of virus-free plants. The expression of SAI and NI were mainly up-regnlated in leaves and immature internedes of virus-free plants at maturation stage, which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants. It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period, and might facilitate the increase of plant biomass.展开更多
文摘The effects of apple dwarf stocks P22 and SH3 on the juvenile phase of Fuji seedlings were studied. The results showed that the juvenile phase could be shortened by more than two years when 2-year seedlings were grafted on the dwarf stocks. The effects of P22 and SH3 were significantly different. Grafted on P22, the flowering node was less, the first flowing site was lower, and the juvenile phase was shorter than SH3. With the two stocks, the average first flowering site was more than 200 cm, the average flowering node was more than 100, and the lateral branch top bud was more than 50% in all first flowing seedlings.
基金Supported by the Project of Hebei Provincial Administration of Quality and Technical Supervision(DB 13/2399-2016)China Agriculture Research System for Apple(CARS-28)~~
文摘The production technique of apple hybrid seedlings was systematically summarized, and the technology regulations were made, including rootstock cultivation, interstock grafting, hybrid seeds preparation and hybrid seedlings grafting. This technical specification provides a unified technical specification for hybrid apple seedling breeders, and is of important guiding significance for the production of apple in Hebei Province.
基金supported by the National Natural Science Foundation of China (Grant No. 31672104)China Agriculture Research System of MOF and MARA (Grant No. CARS-27)+4 种基金Shandong Agricultural Major Applied Technology Innovation Project (Grant No. SD2019ZZ008)Taishan Scholar Funded Project(Grant No. 20190923)Qingchuang Science and Technology Support Project of Shandong Colleges and Universities (Grant No.2019KJF020)Natural Science Foundation of Shandong Province(Grant No. ZR2020MC131)the National Key Research and Development Program of China (Grant No. 2020YFD1000201)。
文摘This study investigated the effects of six crop rotation combinations on the soil quality of old apple orchard and seedling growth of Malus hupehensis Rehd.(apple rootstock) under pot conditions. The inhibitory effects of crops such as Allium fistulosum, Brassica juncea, and Triticum aestivum on four species of Fusarium were observed and compared in six treatments. These were continuous cropping(CK), fumigation with the methyl bromide(FM), rotating A. fistulosum only(R1), rotating A. fistulosum and T. aestivum(R2), rotating A. fistulosum, B. juncea, and T. aestivum(R3), and fallow(FC) in a year. The results showed that the biomass of Malus hupehensis Rehd. seedlings increased significantly. The root length increased and the root architecture was optimized. The respiration rate of the root system was increased by about 1 time after rotation. The treatments of R1, R2, R3, and FC increased bacterial count by 232.17%, 96.04%, 316.21%, and 60.02%, respectively. However, the fungi were reduced in varying degrees and bacteria/fungi ratio was increased by 5–10 times. The enzyme activities, p H, and organic matter were increased, but soil bulk density was decreased. Phenolic acids such as phloridzin was decreased significantly. The copy number of four Fusarium species declined by 85.59%, 74.94%, 69.68%, and 54.41% after rotating three different crops(R3 treatment). The root volatiles of three plants inhibited mycelial growth and spore germination of four Fusarium species.
文摘Shoot-tip culture of strawberry is the most important way to get virus-free plants.Moreover,detoxification and rapid propagation are the key techniques for producing high-quality seeds and seedlings massively and rapidly and achieving the high-quality,high-yield and high-efficiency development of strawberry industry.We have recently produced virus-free strawberry seedlings by combing tissue culture technology and stolon-reproduction method and this technology has been widely used.In addition,a relatively perfect three breeding system of virus-free strawberry seedlings was established after production practices,guaranteeing the supply of high-quality seeds and seedlings and technical support for strawberry industry in corps.
基金Supported by Youth Science Foundation of Guangxi ( Guikeqing0832060)S&T Development Project from Guangxi Academy of Agricultural Sciences(2006006)~~
文摘By employing temporary immersion bioreactor system(TIBs),we studied virus-free culture of seedlings from sugarcane varieties ROC16 and ROC22,from medium recipe,inoculation amount,sucrose concentration,and variety difference. The results showed,using this method,that proliferation rate of ROC16 improved by 40 times,per flask generated about 800 plantlets; of ROC22 improved by 30 times,per flask generated about 400-600 plantlets. The results provided basis for using TIBs in rapid propagation of plantlets via tissue culture.
文摘[ Objective] This study aimed to investigate the tissue culture and rapid propagation techniques of Benihoppe strawberry. [ Method] Shoot tips of new stolons of Benihoppe strawberry were used as experimental materials to analyze the effects of media type, cytokinin type and concentration, carbon source type and concentration, and light intensity on tissue culture and propagation of Benihoppe strawberry. [ Result] MS was the optimal media for plantlet propagation. In the media containing 1.2 ing/L BA （with addition of 0.1 mg/L NAA）, fresh weight, dry weight and propagation coefficient of strawberry plantlets reached the maxi- mum, which were 2.259 g, 0. 221 g and 12.4, respectively. The optimal carbon source was 30 g/L sucrose, and the optimal light intensity was 1 600 lx. [ Conclu- sion] The best media for tissue culture and rapid propagation of Benihoppe strawberry was MS ＋ BA 1.2 mg/L ＋ NAA 0. 1 mg/L ＋ sucrose 30 g/L ＋ agar 8 g/L. This study provided scientific basis for large-scale production of Benihoppe strawberry.
基金Supported by"863"Program(2013AA102604-1)Natural Science Foundation of Hainan Province(20163124)+1 种基金Basal Research Fund for Central Public-interest Scientific Institute(ITBB140503)Earmarked Fund for China Agriculture Research System(CARS-20-2-5)
文摘Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings, and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation. In this study, the differences in the expression levels of 3 invertase genes, CWI, SAI and NI, between virus- free and ordinary sugarcane seedlings were analyzed. Compared with ordinary sugarcane plants, the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage, and especially, greatly up-regulated in immature interuedes at maturation stage of virus-free plants. The expression of SAI and NI were mainly up-regnlated in leaves and immature internedes of virus-free plants at maturation stage, which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants. It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period, and might facilitate the increase of plant biomass.
